Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,162 G→C 5.4% V348V (GTG→GCC RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,1620G→C 5.4% 22.7 ‑0.9 ‑0.6240V348V (GTG→GTCRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/8):  ref base (122/105):  total (127/113)
Fisher's exact test strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.54e-01

Only 100 of 262 total aligned reads displayed.

AAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1397062‑1397261
                                                                                                     |                                                                                                    
 aaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTgc                                                                                                     <  1:3750697/101‑1 (MQ=255)
  aCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTgcg                                                                                                    <  2:4489531/101‑1 (MQ=255)
   cTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTgcgc                                                                                                   <  2:2068872/101‑1 (MQ=255)
    tGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGcc                                                                                                  <  1:859195/101‑1 (MQ=255)
     gCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCg                                                                                                 >  2:2061207/1‑101 (MQ=255)
          cTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCt                                                                                            >  2:3749298/1‑101 (MQ=255)
          cTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCCAAACGTCCAGCTCGCGCCACATGTCTTCAACC.GTGCGCCGTCGCt                                                                                            >  1:1384961/1‑101 (MQ=255)
           ttGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTc                                                                                           >  1:4250795/1‑101 (MQ=255)
           ttGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTc                                                                                           >  2:2121354/1‑101 (MQ=255)
             gAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACGTCTAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccc                                                                                         >  1:5000376/1‑101 (MQ=255)
             gAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccc                                                                                         >  1:209789/1‑101 (MQ=255)
              aGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccc                                                                                        <  2:230010/101‑1 (MQ=255)
              aGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccc                                                                                        >  2:2472346/1‑101 (MQ=255)
                tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCTCGCCACATGTCTTCGACC.CTGCTCCGTCGCTaccccc                                                                                      >  2:3350553/1‑101 (MQ=38)
                tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccccc                                                                                      <  1:3390378/101‑1 (MQ=255)
                tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccccc                                                                                      >  2:4272494/1‑101 (MQ=255)
                tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccccc                                                                                      <  2:1263458/101‑1 (MQ=255)
                tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGGCTTAGACC.GTGCGCCGTCGCTcccccc                                                                                      >  2:4561740/1‑101 (MQ=255)
                 gATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcg                                                                                     <  1:3244958/101‑1 (MQ=255)
                 gATGCGTCGCGAGCACATGTCGTCAATCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcg                                                                                     >  2:157543/1‑101 (MQ=38)
                  aTGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcgc                                                                                    >  2:3674276/1‑101 (MQ=255)
                    ggggCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCgg                                                                                  >  1:2818280/1‑101 (MQ=255)
                    ggggCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCgg                                                                                  >  1:4049677/1‑101 (MQ=255)
                    ggggCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCgg                                                                                  >  2:2008018/1‑101 (MQ=255)
                     gggCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCggg                                                                                 >  2:1729476/1‑101 (MQ=255)
                        cgcgAGCACATGTCTTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGc                                                                              >  1:4731374/1‑101 (MQ=255)
                        cgcgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGc                                                                              <  2:3871027/101‑1 (MQ=255)
                         gcgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCg                                                                             <  1:3896262/101‑1 (MQ=255)
                           gAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                                           >  1:3182386/1‑101 (MQ=255)
                           gAGCACATGTCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                                           <  1:1004999/101‑1 (MQ=255)
                           gAGCACATGTCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                                           <  2:375151/101‑1 (MQ=255)
                            aGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                                          <  1:439382/101‑1 (MQ=255)
                            aGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                                          <  1:1392007/101‑1 (MQ=255)
                            aGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                                          <  2:4690403/101‑1 (MQ=255)
                            aGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                                          <  1:1612848/101‑1 (MQ=255)
                              cccaTGTCTTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        <  2:4969115/99‑1 (MQ=255)
                               acaTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       <  1:1116298/101‑1 (MQ=255)
                                gctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  1:3621198/95‑1 (MQ=0)
                                gctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  2:1961055/95‑1 (MQ=0)
                                gctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      >  1:1007183/7‑101 (MQ=0)
                                caTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  1:583385/101‑1 (MQ=255)
                                caTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      >  1:3945927/1‑101 (MQ=255)
                                 ctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     <  2:2718213/96‑1 (MQ=2)
                                  tgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  2:2101507/97‑1 (MQ=2)
                                   gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   >  2:794495/1‑101 (MQ=255)
                                     cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                                 >  2:4412395/1‑101 (MQ=255)
                                       tcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtg                                                               >  1:3625825/1‑101 (MQ=255)
                                        cgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtgt                                                              >  1:2085525/1‑101 (MQ=255)
                                        cgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtgt                                                              <  1:2008162/101‑1 (MQ=255)
                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTc                                                             <  1:460787/101‑1 (MQ=255)
                                         gCTCCCCCCGCGGGGGCGGGGATCGAAACGTCCAGCTCTCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTc                                                             <  2:1822916/101‑1 (MQ=255)
                                          ccccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCt                                                            <  2:3627888/99‑1 (MQ=255)
                                           tCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTg                                                           >  1:2381713/1‑101 (MQ=255)
                                            ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGc                                                          >  2:1528964/1‑101 (MQ=255)
                                             cccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGcc                                                         <  1:2597987/101‑1 (MQ=255)
                                             cccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGcc                                                         <  1:1824189/101‑1 (MQ=255)
                                             cccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGcc                                                         <  2:2536589/101‑1 (MQ=255)
                                               tccgatctggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.c                                                      <  2:2973607/93‑1 (MQ=255)
                                                  gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgac                                                   <  2:1868013/101‑1 (MQ=255)
                                                  gcgGGGGCGTGGATCGAAACGTCCAGCTCCCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgac                                                   <  2:635821/101‑1 (MQ=255)
                                                   cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgacg                                                  >  2:5015531/1‑101 (MQ=255)
                                                   cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgacg                                                  <  2:3303889/101‑1 (MQ=255)
                                                     ggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGcc                                                <  1:2934379/101‑1 (MQ=255)
                                                       ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCt                                              <  1:4513779/101‑1 (MQ=255)
                                                       ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGTGTCTGCCa.cagatcgga                                              >  1:2973835/1‑93 (MQ=255)
                                                         cGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGc                                            >  1:2239324/1‑101 (MQ=255)
                                                          gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGcc                                           >  1:4090774/1‑100 (MQ=255)
                                                            ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCAcc                                         >  1:3494380/1‑101 (MQ=255)
                                                            ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCAcc                                         >  2:4822559/1‑101 (MQ=255)
                                                              aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAg                                       <  2:1626791/101‑1 (MQ=255)
                                                                cGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGAt                                     >  2:2934531/1‑101 (MQ=255)
                                                                   aaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcg                                  >  1:1659312/1‑101 (MQ=255)
                                                                   aaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcg                                  <  2:3055253/101‑1 (MQ=255)
                                                                     cGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcgcg                                >  1:3836747/1‑101 (MQ=255)
                                                                         cAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTCGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGt                            <  2:4365905/101‑1 (MQ=255)
                                                                          aGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTTCACCAGATCCGCGCCGTc                           <  2:1101458/101‑1 (MQ=255)
                                                                              cgcgCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTc                       <  1:157559/101‑1 (MQ=255)
                                                                               gccgcagaTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCttc                      <  2:1384835/94‑3 (MQ=255)
                                                                               ccgcCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTcc                      <  2:4199860/100‑1 (MQ=255)
                                                                                 gcCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTcccc                    <  2:4090943/101‑1 (MQ=255)
                                                                                 gcCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTcccc                    >  2:644921/1‑101 (MQ=255)
                                                                                  ccACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTccccc                   >  2:4118559/1‑101 (MQ=255)
                                                                                   cacaTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTcccccc                  <  2:1011627/101‑1 (MQ=255)
                                                                                      aTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGTCCCCCTGCACCAGATCCGCGCCGTCGCTCCCCCcgcg               >  2:4613510/1‑101 (MQ=255)
                                                                                       tGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCgg              <  2:4049374/101‑1 (MQ=255)
                                                                                       tGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACACGATCCGCCCCGTCGCTCCCCCCGCgg              >  2:3438044/1‑101 (MQ=255)
                                                                                        gTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggg             >  1:1898040/1‑101 (MQ=255)
                                                                                          cTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggggg           <  2:3802512/101‑1 (MQ=255)
                                                                                          cTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggggg           <  1:669094/101‑1 (MQ=255)
                                                                                           ttCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCT.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGc          <  1:2472520/101‑1 (MQ=255)
                                                                                           ttCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGc          <  1:2336532/101‑1 (MQ=255)
                                                                                            tCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCg         >  2:396830/1‑101 (MQ=255)
                                                                                              gACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTg       >  1:1256218/1‑101 (MQ=255)
                                                                                               aCC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTgg      <  2:2652133/101‑1 (MQ=255)
                                                                                                 c.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt    >  2:2838731/1‑101 (MQ=255)
                                                                                                 c.GTGCGCCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt    >  1:755149/1‑101 (MQ=255)
                                                                                                   gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc   <  2:3129769/101‑1 (MQ=255)
                                                                                                   gTGCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc   <  1:3430101/101‑1 (MQ=255)
                                                                                                   gTGCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc   <  1:1156593/101‑1 (MQ=255)
                                                                                                     gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCg  >  2:2421776/1‑101 (MQ=2)
                                                                                                     |                                                                                                    
AAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1397062‑1397261

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG