Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,213 G→A 2.8% T365T (ACG→CCA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2130G→A 2.8% 7.3 ‑0.4 ‑0.1214T365T (ACG→ACA) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/2):  ref base (114/92):  total (120/94)
Fisher's exact test strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.41e-01

Only 100 of 239 total aligned reads displayed.

CGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCG  >  NC_014034/1397113‑1397313
                                                                                                     |                                                                                                    
cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgacg                                                                                                      <  2:3303889/101‑1 (MQ=255)
 gggggCGTGGATCGAAACGTACAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTATGCCA.CGACGc                                                                                                     >  2:454091/1‑101 (MQ=255)
  ggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGcc                                                                                                    <  1:2934379/101‑1 (MQ=255)
    ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCt                                                                                                  <  2:3031843/101‑1 (MQ=255)
    ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACGTGTCTGCCA.CGACGCCCt                                                                                                  >  2:3651674/1‑101 (MQ=255)
      cGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCC.CGACGCCCTGc                                                                                                >  2:3429837/1‑101 (MQ=255)
       gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCa                                                                                               <  2:641389/101‑1 (MQ=255)
        tGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGCCGCCCTGCAc                                                                                              >  2:4490740/1‑101 (MQ=255)
        tGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCAc                                                                                              <  2:2505472/101‑1 (MQ=255)
         ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCAcc                                                                                             >  2:1794322/1‑101 (MQ=255)
         ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCAcc                                                                                             >  2:4822559/1‑101 (MQ=255)
         ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCAcc                                                                                             >  1:3494380/1‑101 (MQ=255)
             ctaaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGAt                                                                                         <  2:137805/99‑1 (MQ=255)
             cGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGAt                                                                                         >  2:2934531/1‑101 (MQ=255)
              gAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCCGCACCAGATc                                                                                        >  2:3728104/1‑101 (MQ=255)
              gAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCCGCCA.CGACGCCCTGCACCAGATc                                                                                        <  1:4063160/101‑1 (MQ=255)
               aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATcc                                                                                       >  2:4166278/1‑101 (MQ=255)
                aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcg                                                                                      >  2:2336368/1‑101 (MQ=255)
                aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcg                                                                                      <  2:3055253/101‑1 (MQ=255)
                aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcg                                                                                      >  1:1659312/1‑101 (MQ=255)
                aaCGCCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCC.CGACGCCCTGCCCCAGATCcg                                                                                      >  2:1021273/1‑101 (MQ=255)
                  cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcgcg                                                                                    >  2:4287017/1‑101 (MQ=255)
                   gTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCgcgc                                                                                   >  1:4826760/1‑101 (MQ=255)
                     ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCg                                                                                 >  1:75612/1‑101 (MQ=255)
                     ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCg                                                                                 >  1:1237250/1‑101 (MQ=255)
                       aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTTCACCAGATCCGCGCCGTc                                                                               <  2:1101458/101‑1 (MQ=255)
                       aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTc                                                                               >  1:3940210/1‑101 (MQ=255)
                       aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTc                                                                               <  2:4090471/101‑1 (MQ=255)
                        gCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCg                                                                              >  1:3566868/1‑101 (MQ=255)
                         cTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGc                                                                             >  1:4535555/1‑101 (MQ=255)
                          tCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCt                                                                            >  1:2883658/1‑101 (MQ=255)
                           cgcgCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTc                                                                           <  1:157559/101‑1 (MQ=255)
                           cgcgCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTc                                                                           <  2:1924067/101‑1 (MQ=255)
                               ccACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTccccc                                                                       >  2:4118559/1‑101 (MQ=255)
                                 acaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCcg                                                                     <  2:4697571/101‑1 (MQ=255)
                                 acaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCcg                                                                     >  1:3799955/1‑101 (MQ=255)
                                     gTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggg                                                                 >  1:1898040/1‑101 (MQ=255)
                                       cTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggggg                                                               <  1:669094/101‑1 (MQ=255)
                                        ttCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCT.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGc                                                              <  1:2472520/101‑1 (MQ=255)
                                        ttCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGc                                                              <  1:2336532/101‑1 (MQ=255)
                                           gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                           <  1:4456894/101‑1 (MQ=255)
                                           gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                           >  1:1256218/1‑101 (MQ=255)
                                            cccGTGCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                          <  1:1763963/100‑1 (MQ=255)
                                               gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                       <  2:2948827/101‑1 (MQ=255)
                                               gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                       >  2:3795505/1‑101 (MQ=255)
                                               gTGCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                       <  1:3430101/101‑1 (MQ=255)
                                                 gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                      >  2:939820/1‑101 (MQ=2)
                                                   gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                   >  1:2593499/1‑101 (MQ=255)
                                                   gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGACCTGCACCAGATCCGCGCAGTCGCTCCCACCGCGGGGGCGTGGATCGaaa                                                   >  1:2361838/1‑101 (MQ=255)
                                                    ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                  >  1:3286482/1‑101 (MQ=255)
                                                    ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCACTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                  <  1:5015861/101‑1 (MQ=255)
                                                     cGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                 <  2:3977048/101‑1 (MQ=255)
                                                      gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                <  1:1529094/101‑1 (MQ=255)
                                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                               >  2:1185282/1‑101 (MQ=255)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcg                                              <  2:429819/101‑1 (MQ=255)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAAGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcg                                              >  1:2069617/1‑101 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                             <  2:2400162/101‑1 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCACATCCGCGCCGTCGCTCCCCCAGCGGGGGCGCGGATCGAAACACcgc                                             >  2:264573/1‑101 (MQ=255)
                                                          cTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                            >  1:1222463/1‑101 (MQ=255)
                                                          cTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                            <  2:4975076/101‑1 (MQ=255)
                                                            ccccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCa                                          >  2:2823568/1‑101 (MQ=255)
                                                            ccccccGCGGGGGCGTGGATCGAAACGGGTCGGAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAacgctgctg                                           >  1:2020983/1‑94 (MQ=2)
                                                             cccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAc                                         <  1:1021383/101‑1 (MQ=255)
                                                             cccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAc                                         <  1:2535822/101‑1 (MQ=255)
                                                              ccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcg                                        >  2:672461/1‑101 (MQ=255)
                                                                    gggggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaa                                  >  1:558282/1‑101 (MQ=255)
                                                                     ggggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAAc                                 >  2:840814/1‑101 (MQ=255)
                                                                      gggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                                <  1:2965446/101‑1 (MQ=255)
                                                                       ggCGTGGATCGAAACGTGTCTGTGA.CGACGCCCTGGACCAGATCCGCGGCGTCGCTCCCCCCGCGGGGGCTTGGGTCGAAACCCCGAGCACGCGCGCACgg                               >  2:1635777/1‑101 (MQ=25)
                                                                       ggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                               >  1:2194182/1‑101 (MQ=255)
                                                                        gCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGc                              <  2:2013285/101‑1 (MQ=255)
                                                                          gTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                            >  1:4734618/1‑101 (MQ=255)
                                                                          gTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                            >  2:2435153/1‑101 (MQ=255)
                                                                           tGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTc                           <  2:2113347/101‑1 (MQ=255)
                                                                            ggATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                          <  2:2865678/101‑1 (MQ=255)
                                                                             gATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAg                         >  1:4100097/1‑101 (MQ=255)
                                                                              aTCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGc                        <  2:2710872/101‑1 (MQ=255)
                                                                                cGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc                      >  1:820733/1‑101 (MQ=255)
                                                                                 gAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                     <  1:4287334/101‑1 (MQ=255)
                                                                                 gAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACACCGCGCACGCCCGAACGGCGTCAGcccc                     >  2:1448024/1‑101 (MQ=255)
                                                                                 gAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                     >  2:3831873/1‑101 (MQ=255)
                                                                                  aaaCGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcg                    <  2:4904136/101‑1 (MQ=255)
                                                                                  aaaCGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcg                    >  2:4770789/1‑101 (MQ=255)
                                                                                  aaaCGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcg                    >  1:3586324/1‑101 (MQ=255)
                                                                                   accGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcgc                   <  2:3083847/99‑1 (MQ=255)
                                                                                   aaCGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcgc                   >  2:724696/1‑101 (MQ=255)
                                                                                   aaCGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcgc                   >  1:1082150/1‑101 (MQ=255)
                                                                                    aCGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcgcg                  <  2:3239232/101‑1 (MQ=255)
                                                                                     cGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGa                 >  2:1574812/1‑101 (MQ=255)
                                                                                       tgtCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc               >  2:1209611/1‑101 (MQ=255)
                                                                                       tgtCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc               <  2:2759368/101‑1 (MQ=255)
                                                                                        gtCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGAACGCGCGAACGGCGTCAGCCCCGCGAACg              >  2:3897731/1‑101 (MQ=255)
                                                                                         tCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGt             >  1:2123916/1‑101 (MQ=255)
                                                                                          cTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTc            <  2:4349645/101‑1 (MQ=255)
                                                                                          cTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTc            >  1:1146969/1‑101 (MQ=255)
                                                                                          cTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCAGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACgcc            >  2:2576203/1‑99 (MQ=255)
                                                                                           tGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg           >  1:1445910/1‑101 (MQ=255)
                                                                                            gCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGGCGc          >  2:1543177/1‑101 (MQ=255)
                                                                                            gCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCACACGCGCGAACGGCGTCAGCCCCGCGAACGTCGc          >  1:4332480/1‑101 (MQ=255)
                                                                                                  gacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccc     <  2:3622524/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
CGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCG  >  NC_014034/1397113‑1397313

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG