Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,539 T→C 13.3% I474T (ATC→GCC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5390T→C13.3% 69.2 ‑0.8 ‑0.7310I474T (ATC→ACC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (13/17):  ref base (122/112):  total (156/154)
Fisher's exact test strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.21e-01

Only 100 of 329 total aligned reads displayed.

TCCCCCCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTC  >  NC_014034/1397439‑1397639
                                                                                                    |                                                                                                          
tCCCCCCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGAt                                                                                                            >  1:3614242/1‑101 (MQ=255)
 ccccccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATc                                                                                                           >  2:538367/1‑101 (MQ=255)
 ccccccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATc                                                                                                           <  1:1980732/101‑1 (MQ=255)
   ccccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCAt                                                                                                         >  1:4968038/1‑101 (MQ=255)
   ccccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCAt                                                                                                         <  1:1597565/101‑1 (MQ=255)
    cccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATg                                                                                                        <  2:2869756/101‑1 (MQ=255)
         gggggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..Cg                                                                                               >  1:1552364/1‑101 (MQ=255)
         gggggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACAACCCGATCATGG.C.C..Cg                                                                                               >  1:1654780/1‑101 (MQ=255)
           gggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGcc                                                                                             <  1:934735/101‑1 (MQ=255)
             gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTc                                                                                           <  2:713565/101‑1 (MQ=255)
             gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTc                                                                                           <  2:4066655/101‑1 (MQ=255)
              cGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCa                                                                                          >  1:4827998/1‑101 (MQ=255)
               gTGGATCGAAACACCGTCATCACCACGCACCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAg                                                                                         <  2:1082396/101‑1 (MQ=255)
               gTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAg                                                                                         <  2:3016047/101‑1 (MQ=255)
                 ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAgcg                                                                                       >  2:3144670/1‑101 (MQ=255)
                    tCGAAACACCGTCATCACCACGCAACCGCCCTGCTCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGcgccg                                                                                    <  1:4332770/101‑1 (MQ=255)
                        aaCACCGTCATCACCGCGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCt                                                                                <  2:5046505/101‑1 (MQ=255)
                         acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCta                                                                               >  1:1481194/1‑100 (MQ=255)
                         acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.g                                                                              <  1:592471/101‑1 (MQ=255)
                         acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.g                                                                              >  2:3511361/1‑101 (MQ=255)
                              gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTc                                                                        <  2:1067109/101‑1 (MQ=255)
                                 atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCCCt                                                                     >  1:1037019/1‑101 (MQ=255)
                                    accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccc                                                                  >  1:1000175/1‑101 (MQ=255)
                                      cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccccc                                                                <  2:1692184/101‑1 (MQ=255)
                                       acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccccc                                                               <  1:2079551/101‑1 (MQ=255)
                                         gCACCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                             <  2:786732/101‑1 (MQ=255)
                                         gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                             <  1:116220/101‑1 (MQ=255)
                                         gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                             >  1:1849373/1‑101 (MQ=255)
                                         gCAACCACCCTGGACCTCCGGTCGCCCCCCCCGCGGAGGCGTGAGACGAAACACCCCGACCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                             <  2:2139174/101‑1 (MQ=255)
                                            aCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCTTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCggg                                                          >  1:4354573/1‑101 (MQ=255)
                                            aCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCggg                                                          >  2:323002/1‑101 (MQ=255)
                                             ccGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgggg                                                         >  2:4000569/1‑101 (MQ=255)
                                               gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                       <  2:2543480/101‑1 (MQ=255)
                                                 ccTGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGt                                                     <  2:3310703/101‑1 (MQ=255)
                                                   tGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                   <  2:441107/101‑1 (MQ=255)
                                                   ctcaccacCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                   <  2:466051/99‑1 (MQ=255)
                                                    tgccgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                  <  1:4013275/92‑1 (MQ=2)
                                                    gCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                  >  2:2564711/1‑101 (MQ=255)
                                                     aacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                <  1:3547305/93‑1 (MQ=2)
                                                      accaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                >  2:636996/1‑101 (MQ=255)
                                                       ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                               <  2:1222346/101‑1 (MQ=255)
                                                       ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                               <  2:59962/101‑1 (MQ=255)
                                                        caccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                              <  2:3088297/101‑1 (MQ=255)
                                                        aggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             <  1:4619179/96‑1 (MQ=2)
                                                         gagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                              >  1:4539078/5‑101 (MQ=2)
                                                         cccGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAACCCGCTCCTGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             <  1:5039333/100‑1 (MQ=255)
                                                         accGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             >  2:3196296/1‑101 (MQ=255)
                                                          tgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                            <  1:4694603/98‑1 (MQ=2)
                                                          tgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                            <  1:3873980/98‑1 (MQ=2)
                                                          gatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGCGTGGATCGAAAc                                           >  1:4187055/4‑101 (MQ=2)
                                                           tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GA.T.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAc                                           >  1:1390546/2‑101 (MQ=2)
                                                           cGGTCGCTCCCCCGGGGGGGGCGTGGATCGAAACAA.CTGACCCGGGAA.C..GGCCTGTGCGCTG.GAC.GT.GTTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                          <  2:1815864/101‑2 (MQ=2)
                                                            ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                          >  1:2937739/1‑101 (MQ=255)
                                                            cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                           >  1:3283097/2‑101 (MQ=2)
                                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                         >  1:2201262/1‑101 (MQ=2)
                                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                        <  1:3137738/101‑1 (MQ=2)
                                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtc                                      <  1:2544229/101‑6 (MQ=2)
                                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGCGGGGGTCGAAAcggggc                                      >  1:3827155/1‑96 (MQ=0)
                                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                       >  1:3490358/1‑101 (MQ=255)
                                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                     <  2:1481213/101‑7 (MQ=2)
                                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                       <  1:2779696/101‑4 (MQ=2)
                                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                       >  1:4126686/1‑98 (MQ=2)
                                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                       <  1:709721/101‑4 (MQ=2)
                                                                  tCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgcg                                    >  1:1272581/1‑101 (MQ=255)
                                                                  tCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgcg                                    <  1:1421333/101‑1 (MQ=255)
                                                                  cccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaa                                   <  1:2141492/100‑9 (MQ=2)
                                                                  cccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaa                                   <  2:4572235/100‑9 (MQ=2)
                                                                   ccccccGCGGGGGCGTGGATCGAAACTTGATGACCA.GA.T.C..CGCAACCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcctatgagg                                   <  1:4244618/101‑9 (MQ=0)
                                                                   ccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaag                                  <  2:4315229/101‑10 (MQ=2)
                                                                   ccccccGCGGGGGCGTGGATCGAAACACCCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                   <  2:1381206/101‑1 (MQ=255)
                                                                   ccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatga                                   >  2:1611318/1‑96 (MQ=2)
                                                                   ccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                    >  2:828414/1‑95 (MQ=0)
                                                                   ccccccGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                   >  2:2427522/1‑101 (MQ=255)
                                                                   ccccccGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCCGCgg                                   >  2:4934411/1‑101 (MQ=255)
                                                                    cccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                  <  2:4330042/101‑7 (MQ=2)
                                                                     ccccGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAATCCGCGGAt                                 >  2:222497/1‑101 (MQ=255)
                                                                       ccGGGGGGGGGTGGATCGAAACAACCCGGTCTTGT.C.C..CGCTTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCg                               <  2:1545429/101‑1 (MQ=37)
                                                                       ccGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCg                               >  1:4919977/1‑101 (MQ=255)
                                                                         gcaggggCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGc                             >  2:4785026/4‑101 (MQ=255)
                                                                          cgGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                            >  2:3915795/1‑101 (MQ=255)
                                                                              ggCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                        >  1:1775533/1‑101 (MQ=255)
                                                                               gCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcc                       <  1:4386739/101‑1 (MQ=255)
                                                                                cGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                      >  1:3356792/1‑101 (MQ=255)
                                                                                  tGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                    >  2:1904895/1‑101 (MQ=255)
                                                                                  tGGATCAAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                    <  1:829236/101‑1 (MQ=255)
                                                                                    gATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                  <  1:3221149/101‑1 (MQ=255)
                                                                                     aTCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                 >  1:2632627/1‑101 (MQ=255)
                                                                                      tCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCTCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                <  2:3115722/101‑1 (MQ=255)
                                                                                      tCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                <  2:3610050/101‑1 (MQ=255)
                                                                                       agcatagttccGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccgc               >  2:4341198/10‑101 (MQ=255)
                                                                                        gAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc              >  1:2877629/1‑101 (MQ=255)
                                                                                         aaccaccccGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc             <  2:380915/95‑1 (MQ=255)
                                                                                         aaaCAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGTCGTGACCcgccccgccc             >  1:2490926/1‑101 (MQ=255)
                                                                                         aaaCAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc             >  2:2347835/1‑101 (MQ=255)
                                                                                         aaaCAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc             <  1:2457202/101‑1 (MQ=255)
                                                                                          aacaacCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCa            <  1:1087479/101‑1 (MQ=255)
                                                                                           acaacCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt           >  2:339404/1‑101 (MQ=255)
                                                                                            caacCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.TGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc          <  2:2937511/101‑1 (MQ=255)
                                                                                              acCCGATCGTGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCgg        <  2:916995/101‑1 (MQ=255)
                                                                                                    tCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTc  >  1:2363042/1‑101 (MQ=255)
                                                                                                    |                                                                                                          
TCCCCCCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTC  >  NC_014034/1397439‑1397639

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG