Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,549 C→G 7.8% R477R (CGC→GGG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5490C→G 7.8% 42.7 ‑0.2 ‑0.0300R477R (CGC→CGG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (12/10):  ref base (133/123):  total (152/148)
Fisher's exact test strand distribution p-value = 8.29e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.90e-01

Only 100 of 323 total aligned reads displayed.

GGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGG  >  NC_014034/1397449‑1397649
                                                                                                       |                                                                                                    
ggggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGc                                                                                                      >  2:4628928/1‑101 (MQ=255)
 gggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGcc                                                                                                     <  2:4325798/101‑1 (MQ=255)
 gggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGcc                                                                                                     <  2:1066119/101‑1 (MQ=255)
  ggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCt                                                                                                    <  1:1399604/101‑1 (MQ=255)
  ggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCt                                                                                                    <  1:4727618/101‑1 (MQ=255)
   gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTc                                                                                                   <  2:1407067/101‑1 (MQ=255)
   gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTc                                                                                                   <  2:713565/101‑1 (MQ=255)
   gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTc                                                                                                   >  1:1556847/1‑101 (MQ=255)
   gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTc                                                                                                   >  2:4023649/1‑101 (MQ=255)
    cGTGGATCGAAACCCCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCa                                                                                                  <  1:1436321/101‑1 (MQ=255)
    cGTGGATCGAAACCCCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCa                                                                                                  <  2:3115818/101‑1 (MQ=255)
     gTGGATCGAAACACCGTCATCACCACGCACCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAg                                                                                                 <  2:1082396/101‑1 (MQ=255)
     gTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAg                                                                                                 >  1:3748822/1‑101 (MQ=255)
       ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAgcg                                                                                               <  2:2544071/101‑1 (MQ=255)
       ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGACCATGGC.C..CGCCTCAgcc                                                                                               >  2:3286589/1‑100 (MQ=255)
          tCGAAACACCGTCATCACCACGCAACCGCCCTGCTCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGcgccg                                                                                            <  1:4332770/101‑1 (MQ=255)
           cGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAgcggccg                                                                                           >  2:2889924/1‑97 (MQ=255)
            gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCcgcg                                                                                          <  1:4576817/101‑1 (MQ=255)
             aaaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCgcgc                                                                                         >  2:4386209/1‑101 (MQ=255)
              aaCACCGTCATCACCGCGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCt                                                                                        <  2:5046505/101‑1 (MQ=255)
               acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCta                                                                                       >  1:1481194/1‑100 (MQ=255)
               acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTg                                                                                       >  2:3511361/1‑101 (MQ=255)
               acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTg                                                                                       <  2:1581781/101‑1 (MQ=255)
                      catcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGc                                                                                <  2:3183185/101‑1 (MQ=255)
                       atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCt                                                                               >  1:671960/1‑101 (MQ=255)
                       atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCCCt                                                                               >  1:1037019/1‑101 (MQ=255)
                       atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCACt                                                                               >  2:4257180/1‑101 (MQ=255)
                        tcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTc                                                                              >  1:3570757/1‑101 (MQ=255)
                         caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTcc                                                                             >  1:201059/1‑101 (MQ=255)
                          accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTccc                                                                            >  1:1000175/1‑101 (MQ=255)
                               gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgc                                                                       <  1:116220/101‑1 (MQ=255)
                               gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgc                                                                       >  1:1849373/1‑101 (MQ=255)
                               gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgc                                                                       >  1:498381/1‑101 (MQ=255)
                                  aCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCTTCAGCGCCGCGCTGACGTCGCTCCCCCCGCggg                                                                    >  1:4354573/1‑101 (MQ=255)
                                    cGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCggggg                                                                  <  2:488465/101‑1 (MQ=255)
                                     gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGc                                                                 >  2:625108/1‑101 (MQ=255)
                                       ccTGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGt                                                               <  2:3310703/101‑1 (MQ=255)
                                        cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTg                                                              >  1:517973/1‑101 (MQ=255)
                                           aacaggatgTCGCACCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGGCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  1:3367032/93‑1 (MQ=0)
                                            atcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                           <  2:306359/94‑1 (MQ=0)
                                            accaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                          >  1:2384175/1‑101 (MQ=255)
                                            accaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                          >  1:384980/1‑101 (MQ=255)
                                             cgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         >  1:3940410/7‑101 (MQ=2)
                                             ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         <  1:4023952/101‑1 (MQ=255)
                                              gctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGCTACCGCGTCGCTCCCCCCGCGGGGGCGGGGATCGa                                                        >  2:2011300/6‑101 (MQ=2)
                                              cgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         <  2:82037/96‑1 (MQ=0)
                                              caccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                        <  2:4728789/101‑1 (MQ=255)
                                              caccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCAGGGC.C..CGCCTCAGCGCCGGGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                        >  2:223573/1‑101 (MQ=255)
                                               accGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       >  1:4663576/1‑101 (MQ=255)
                                                ccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      <  1:2138012/101‑1 (MQ=255)
                                                 cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGCGGCGTGGATCGAAAc                                                     <  1:4260519/101‑1 (MQ=255)
                                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                    >  1:2937739/1‑101 (MQ=255)
                                                  cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGGAAc                                                     >  2:1797442/2‑101 (MQ=2)
                                                  cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                     <  2:2942388/100‑1 (MQ=2)
                                                  cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                     >  1:3283097/2‑101 (MQ=2)
                                                   gTCGCTCCCCCCGGGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACAt                                                   >  1:2483848/1‑101 (MQ=2)
                                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                   >  1:26221/1‑101 (MQ=2)
                                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                   <  2:1176851/101‑2 (MQ=2)
                                                    tCGCCCCCCCCGCGGGGGCGTGGACCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                  <  2:2349862/101‑1 (MQ=2)
                                                    gcGCTCCCCCCGCTGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                  <  2:2507836/100‑1 (MQ=255)
                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                                 >  2:3090468/1‑101 (MQ=255)
                                                      tccccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                 <  1:872245/98‑4 (MQ=0)
                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                               <  1:4200446/101‑7 (MQ=2)
                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATcga                                                >  2:4504788/1‑99 (MQ=2)
                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                 <  2:895147/101‑4 (MQ=2)
                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGTTCGAAACATCcg                                                <  1:2731161/101‑1 (MQ=255)
                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                >  1:1948376/1‑101 (MQ=255)
                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgc                                               >  2:2672079/1‑101 (MQ=255)
                                                        tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                              <  2:1904474/101‑5 (MQ=2)
                                                        tCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgcg                                              >  1:1272581/1‑101 (MQ=255)
                                                         ccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaag                                            <  2:559775/101‑10 (MQ=2)
                                                         ccccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGCCGTGGATCGAAACATCCGCgg                                             <  1:2871834/101‑1 (MQ=255)
                                                          cccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaaga                                           <  1:1663391/101‑11 (MQ=0)
                                                           ccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                           <  2:2203987/101‑1 (MQ=255)
                                                            cccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                          >  1:4443782/1‑101 (MQ=255)
                                                             ccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCg                                         >  1:4919977/1‑101 (MQ=255)
                                                                cgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                      >  2:3915795/1‑101 (MQ=255)
                                                                  ggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAACGGCGTg                                    >  1:2171610/1‑101 (MQ=255)
                                                                   gggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                                   >  1:2522878/1‑101 (MQ=255)
                                                                    ggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                  >  1:1775533/1‑101 (MQ=255)
                                                                    ggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                  >  2:1533238/1‑101 (MQ=255)
                                                                        tGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGAAGCCCCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                              >  1:4157176/1‑101 (MQ=255)
                                                                         ggATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                             >  1:721314/1‑101 (MQ=255)
                                                                         ggATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGGGAgccgcc                             >  2:164977/1‑101 (MQ=255)
                                                                          gATCGAAACAACCCGATCATGGA.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTCCcccgccc                            >  2:77219/1‑101 (MQ=255)
                                                                           aTCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                           <  1:313681/101‑1 (MQ=255)
                                                                            tCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                          <  2:3610050/101‑1 (MQ=255)
                                                                             agcatagttccGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccgc                         >  2:4341198/10‑101 (MQ=255)
                                                                               aaccaccccGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                       <  2:380915/95‑1 (MQ=255)
                                                                                aacaacCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCa                      <  1:1087479/101‑1 (MQ=255)
                                                                                   aacCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGGGGCCCGCCCCGCCCATCg                   >  2:3202410/1‑101 (MQ=255)
                                                                                      ccGACCAGGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc                <  1:3511631/101‑1 (MQ=255)
                                                                                          tCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTc            <  1:3190181/101‑1 (MQ=255)
                                                                                           cagggC.C..CGCCTCAACGCCGCGCTGAGGCCGCTTCCCCCGCGGGGGCGGGGATCCAAACATCCGCGGATCGGCGGGCGCCGCCCCGCCCGTCCGGCGCttc           >  2:4142152/4‑99 (MQ=255)
                                                                                           cATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc           <  1:4873604/101‑1 (MQ=255)
                                                                                            agggC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGTATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc          <  1:2214594/99‑1 (MQ=255)
                                                                                            aTGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACGTCCTCGGATCGGCCTGAGCCGCCCCACCCCTCgggctcggcc          >  1:348363/1‑93 (MQ=25)
                                                                                              ggC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGCCGCTccccc        >  2:1001806/1‑101 (MQ=255)
                                                                                               gC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc       <  2:3371051/101‑1 (MQ=255)
                                                                                                  c..cGCCTCAGCGCCGCGCTGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgc     <  2:1272460/101‑1 (MQ=255)
                                                                                                       |                                                                                                    
GGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGG  >  NC_014034/1397449‑1397649

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG