Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,553 A→C 9.4% S479R (AGC→CAC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5530A→C 9.4% 59.0 ‑2.2 ‑0.1288S479R (AGC→CGC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (10/17):  ref base (140/121):  total (150/138)
Fisher's exact test strand distribution p-value = 1.10e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.06e-01

Only 100 of 312 total aligned reads displayed.

CGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCG  >  NC_014034/1397453‑1397653
                                                                                                        |                                                                                                      
 gTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAg                                                                                                       <  2:3016047/101‑1 (MQ=255)
     aTCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGcgcc                                                                                                   <  2:713310/101‑1 (MQ=255)
     aTCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGcgcc                                                                                                   >  2:2733580/1‑101 (MQ=255)
     aTCGAAACACCGTCATCACCACGCAACCGACGTGCACCACCGGGCGCTCCCCCCGCGGGCGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGcgcc                                                                                                   <  2:844586/101‑1 (MQ=38)
         aaaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCgcgc                                                                                               >  2:4386209/1‑101 (MQ=255)
         aaaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCgcgc                                                                                               >  1:1600229/1‑101 (MQ=255)
          aaCACCGTCATCACCGCGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCt                                                                                              <  2:5046505/101‑1 (MQ=255)
           acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.g                                                                                            >  2:3511361/1‑101 (MQ=255)
           acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.g                                                                                            <  2:2933300/101‑1 (MQ=255)
             acCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.c                                                                                         >  1:2893178/1‑101 (MQ=255)
                gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTGTTCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTc                                                                                      >  1:3494110/1‑101 (MQ=37)
                  catcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGc                                                                                    <  2:3183185/101‑1 (MQ=255)
                   atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCt                                                                                   <  2:394977/101‑1 (MQ=255)
                   atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCACt                                                                                   >  2:4257180/1‑101 (MQ=255)
                      accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccc                                                                                >  2:3220898/1‑101 (MQ=255)
                      accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccc                                                                                >  1:1000175/1‑101 (MQ=255)
                       ccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccc                                                                               >  2:3326312/1‑101 (MQ=255)
                        cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccccc                                                                              <  2:1692184/101‑1 (MQ=255)
                           gCAACCACCCTGGACCTCCGGTCGCCCCCCCCGCGGAGGCGTGAGACGAAACACCCCGACCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                           <  2:2139174/101‑1 (MQ=255)
                           ccAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                           <  2:109327/100‑1 (MQ=255)
                             aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCCCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgg                                                                         >  1:3122509/1‑101 (MQ=255)
                              aCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCggg                                                                        >  1:1521298/1‑101 (MQ=255)
                                cGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCggggg                                                                      <  2:488465/101‑1 (MQ=255)
                                 gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                     >  2:625108/1‑101 (MQ=255)
                                  cccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCg                                                                    >  2:2224874/1‑101 (MQ=255)
                                   ccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGt                                                                   >  1:1449391/1‑101 (MQ=255)
                                   ccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGGCGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGt                                                                   <  1:4336114/101‑1 (MQ=255)
                                    cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                  >  1:517973/1‑101 (MQ=255)
                                    cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                  <  2:3298291/101‑1 (MQ=255)
                                     ggaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTCGA.C.C..CAGTTCA.CGGCGGGCT.CT.CGTCGCCCCCCCCCCGGGGGCGTGGa                                                                >  1:635273/11‑101 (MQ=0)
                                      tgccgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                                <  1:4013275/92‑1 (MQ=2)
                                         gcgctggTCGCTCCACCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GA.T.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             <  1:4358757/96‑1 (MQ=0)
                                         ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             <  2:1222346/101‑1 (MQ=255)
                                          cgagcgTCTCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             <  1:4184528/96‑1 (MQ=0)
                                          cgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             <  2:82037/96‑1 (MQ=0)
                                          caccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                            <  2:3088297/101‑1 (MQ=255)
                                          caccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                            <  2:4728789/101‑1 (MQ=255)
                                          aggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           <  1:4619179/96‑1 (MQ=2)
                                           gagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                            >  1:4539078/5‑101 (MQ=2)
                                            tgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                          <  1:3873980/98‑1 (MQ=2)
                                            tgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                          <  1:4694603/98‑1 (MQ=2)
                                            ccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                          <  1:2138012/101‑1 (MQ=255)
                                            agcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           <  1:1274871/98‑1 (MQ=2)
                                             cGGTCGCTCCCCCGGGGGGGGCGTGGATCGAAACAA.CTGACCCGGGAA.C..GGCCTGTGCGCTG.GAC.GT.GTTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                        <  2:1815864/101‑2 (MQ=2)
                                              cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                         >  1:3283097/2‑101 (MQ=2)
                                               gTCGCTCCCCCCGGGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACAt                                                       >  1:2483848/1‑101 (MQ=2)
                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGCGGGGGTCGAAAcggg                                                      >  1:1830242/1‑98 (MQ=0)
                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                       >  1:26221/1‑101 (MQ=2)
                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                       >  2:3101903/1‑101 (MQ=255)
                                               gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                       <  1:2403980/101‑1 (MQ=255)
                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcgggg                                                     >  1:2073811/1‑97 (MQ=2)
                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                      >  1:1712485/1‑101 (MQ=255)
                                                gcGCTCCCCCCGCTGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                      <  2:2507836/100‑1 (MQ=255)
                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtc                                                    <  2:2562468/101‑6 (MQ=2)
                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                      <  1:4277967/101‑3 (MQ=2)
                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                                     <  2:3122271/101‑1 (MQ=255)
                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                                     >  1:3557851/1‑101 (MQ=255)
                                                  tccccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                     <  1:872245/98‑4 (MQ=0)
                                                  gCTCGCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcga                                                    >  1:4887783/1‑99 (MQ=2)
                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                                   <  2:1481213/101‑7 (MQ=2)
                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                     <  1:709721/101‑4 (MQ=2)
                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGTTCGAAACATCcg                                                    <  1:2731161/101‑1 (MQ=255)
                                                  cccccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                     <  2:2311084/98‑4 (MQ=0)
                                                    cccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                                   <  1:2815117/100‑6 (MQ=0)
                                                     ccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaag                                                <  2:559775/101‑10 (MQ=2)
                                                     ccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaag                                                <  1:4959908/101‑10 (MQ=2)
                                                     ccccccGCGGGGGCGTGGATCGAAACACCCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                                 <  2:1381206/101‑1 (MQ=255)
                                                     ccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatga                                                 >  2:1611318/1‑96 (MQ=2)
                                                      cccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                                <  2:4330042/101‑7 (MQ=2)
                                                       ccccGGGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                               <  2:3823277/101‑1 (MQ=255)
                                                       ccccGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                               <  1:1411011/101‑1 (MQ=255)
                                                        cccGCGGGGGCGTGGGTCGAAACAACCCGATCATGG.C.C..CGCCCCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                              >  1:380968/1‑101 (MQ=255)
                                                         cggcgGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCg                                             <  1:1218610/99‑1 (MQ=255)
                                                         ccGGGGGGGGGTGGATCGAAACAACCCGGTCTTGT.C.C..CGCTTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCg                                             <  2:1545429/101‑1 (MQ=37)
                                                             gggggCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                         <  1:1091230/101‑1 (MQ=255)
                                                             gggggCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCCGAGCGGCgg                                         >  2:100815/1‑100 (MQ=255)
                                                              ggggCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAACGGCGTg                                        >  1:2171610/1‑101 (MQ=255)
                                                               gggCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                                       >  1:1054237/1‑101 (MQ=255)
                                                               gggCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                                       >  1:2522878/1‑101 (MQ=255)
                                                                ggCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                      >  2:1076117/1‑101 (MQ=255)
                                                                 gCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcc                                     >  1:4209884/1‑101 (MQ=255)
                                                                  cGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                    >  1:3356792/1‑101 (MQ=255)
                                                                    tGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                  >  2:1904895/1‑101 (MQ=255)
                                                                      gATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                >  1:4855283/1‑101 (MQ=255)
                                                                      gATCGAAACAACCCGATCATGG.A.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTCCcccgccc                                >  2:77219/1‑101 (MQ=255)
                                                                        tCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                              <  2:3610050/101‑1 (MQ=255)
                                                                         agcatagttccGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccgc                             >  2:4341198/10‑101 (MQ=255)
                                                                             acaccCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt                         <  1:405277/101‑1 (MQ=255)
                                                                             acaacCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt                         >  2:339404/1‑101 (MQ=255)
                                                                                  ccGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc                    <  2:2384011/101‑1 (MQ=255)
                                                                                    gATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCCTCGGGCGc                  >  2:4573690/1‑101 (MQ=255)
                                                                                     aTCAGGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                 <  1:1957493/101‑1 (MQ=255)
                                                                                      tCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTc                <  1:3190181/101‑1 (MQ=255)
                                                                                       cttGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGTGGTGGATAGAAACATCCGCGGATCGGGGTGACCCGCCCCGCCCATCGGTCGCTcc               <  1:3383903/99‑1 (MQ=38)
                                                                                       caggg.C.C..CGCCTCAACGCCGCGCT.GA.GGCCGCTTCCCCCGCGGGGGCGGGGATCCAAACATCCGCGGATCGGCGGGCGCCGCCCCGCCCGTCCGGCGCttc               >  2:4142152/4‑99 (MQ=255)
                                                                                       cATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc               >  1:3684807/1‑101 (MQ=255)
                                                                                        aggg.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGTATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc              <  1:2214594/99‑1 (MQ=255)
                                                                                        aTGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc              >  1:1867324/1‑101 (MQ=255)
                                                                                                     cTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg     >  2:4469771/1‑101 (MQ=255)
                                                                                                      ccAGCGCCGCTGT.GA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCggggg    <  2:3719039/100‑1 (MQ=255)
                                                                                                        |                                                                                                      
CGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCG  >  NC_014034/1397453‑1397653

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG