Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,560 C→G 12.2% A481G (GCG→GGT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5600C→G12.2% 60.1 ‑2.3 ‑1.5264A481G (GCG→GGG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (10/17):  ref base (104/87):  total (135/129)
Fisher's exact test strand distribution p-value = 1.02e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.18e-02

Only 100 of 313 total aligned reads displayed.

GAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1397461‑1397660
                                                                                                       |                                                                                                      
 aaaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCgcgc                                                                                                      >  2:1253233/1‑101 (MQ=255)
     acCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.c                                                                                                <  2:1397660/101‑1 (MQ=255)
              accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccc                                                                                       >  1:1000175/1‑101 (MQ=255)
              accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccc                                                                                       >  2:4454991/1‑101 (MQ=255)
                   gCACCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                                  <  2:786732/101‑1 (MQ=255)
                   gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                                  >  1:498381/1‑101 (MQ=255)
                   gCAACCACCCTGGACCTCCGGTCGCCCCCCCCGCGGAGGCGTGAGACGAAACACCCCGACCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                                  <  2:2139174/101‑1 (MQ=255)
                     aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgg                                                                                <  2:3609791/101‑1 (MQ=255)
                      aCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCggg                                                                               >  2:323002/1‑101 (MQ=255)
                        cGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCCCcgggg                                                                             >  1:14617/1‑101 (MQ=255)
                         gCCTTGCACCCCCTGTCGCTCCCCCCGCGGGGGAGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                            <  1:4346265/101‑1 (MQ=38)
                          cccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCg                                                                           >  2:2224874/1‑101 (MQ=255)
                           ccTGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGt                                                                          <  2:3310703/101‑1 (MQ=255)
                             tGCCCCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                                        <  2:4029035/101‑1 (MQ=255)
                             ggaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTCGA.C.C..CAGTTCA.CGGCGGGCT.CT.CGTCGCCCCCCCCCCGGGGGCGTGGa                                                                       >  1:635273/11‑101 (MQ=0)
                               aacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  1:3547305/93‑1 (MQ=2)
                               aacaggatgTCGCACCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGGCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  1:3367032/93‑1 (MQ=0)
                                accaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     >  1:384980/1‑101 (MQ=255)
                                accaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     >  1:2384175/1‑101 (MQ=255)
                                accaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.A..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  1:591651/101‑1 (MQ=255)
                                 ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                    <  2:59962/101‑1 (MQ=255)
                                 ccaccGGTCCCCCCCCCCGCGGGGGCGTGGATCGAACCAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                    <  2:1556717/101‑1 (MQ=255)
                                   gagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGCTCGa                                                                   >  2:3681088/5‑101 (MQ=0)
                                   ctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                  >  2:4694280/5‑101 (MQ=2)
                                   accGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                  >  1:4663576/1‑101 (MQ=255)
                                    ccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                 <  1:2138012/101‑1 (MQ=255)
                                    agcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                  <  1:1274871/98‑1 (MQ=2)
                                    agcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                  <  1:2485652/98‑1 (MQ=2)
                                     cGGTCGCTCCCCCGGGGGGGGCGTGGATCGAAACAA.CTGACCCGGGAA.C..GGCCTGTGCGCTG.GAC.GT.GTTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                               <  2:1815864/101‑2 (MQ=2)
                                      ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                               >  2:4316758/1‑101 (MQ=255)
                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGCGGGGGTCGAAAcggg                                                             >  1:1830242/1‑98 (MQ=0)
                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                              >  2:722121/1‑101 (MQ=255)
                                        tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcgggg                                                            >  1:2073811/1‑97 (MQ=2)
                                        tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                             >  1:1524598/1‑101 (MQ=2)
                                        tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                             <  1:3137738/101‑1 (MQ=2)
                                        tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                             >  1:1712485/1‑101 (MQ=255)
                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtc                                                           <  2:2562468/101‑6 (MQ=2)
                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtc                                                           <  1:2544229/101‑6 (MQ=2)
                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                            <  2:1633058/101‑2 (MQ=2)
                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                             <  1:4277967/101‑3 (MQ=2)
                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                                            <  2:3122271/101‑1 (MQ=255)
                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                                          <  2:4884037/101‑7 (MQ=2)
                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                            <  1:2779696/101‑4 (MQ=2)
                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                            <  1:709721/101‑4 (MQ=2)
                                          cccccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                            <  2:2311084/98‑4 (MQ=0)
                                            tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                         >  2:2479082/1‑97 (MQ=2)
                                            tCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                                          >  2:3078866/1‑96 (MQ=0)
                                            cccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCG.A.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaa                                                        <  2:4572235/100‑9 (MQ=2)
                                             ccccccGCGGGGGCGTGGATCGAAACTTGATGACCA.GA.T.C..CGCAACCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcctatgagg                                                        <  1:4244618/101‑9 (MQ=0)
                                             ccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatga                                                        >  1:4566272/1‑96 (MQ=2)
                                             ccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAG.C.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                                         >  2:828414/1‑95 (MQ=0)
                                             ccccccGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGCCGTGGATCGAAACATCCGCgg                                                        <  1:2871834/101‑1 (MQ=255)
                                               ccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGT.CGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatc                                                      >  2:4341196/1‑94 (MQ=2)
                                               ccccGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                                      <  2:3490093/101‑1 (MQ=255)
                                               ccccGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                                      <  1:1411011/101‑1 (MQ=255)
                                                cccGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                                     >  1:4443782/1‑101 (MQ=255)
                                                 cggcgGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCg                                                    <  1:1218610/99‑1 (MQ=255)
                                                 ccGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCg                                                    >  1:4919977/1‑101 (MQ=255)
                                                  cgcgGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GC.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCgg                                                   <  2:4365862/101‑1 (MQ=255)
                                                    cgGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                                 >  2:3915795/1‑101 (MQ=255)
                                                     gggggCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                                <  1:1091230/101‑1 (MQ=255)
                                                     gggggCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCCGAGCGGCgg                                                >  2:100815/1‑100 (MQ=255)
                                                      ggggCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                               <  1:405106/101‑1 (MQ=255)
                                                      ggggCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAACGGCGTg                                               >  1:2171610/1‑101 (MQ=255)
                                                       gggCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                                              >  1:1054237/1‑101 (MQ=255)
                                                        ggCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                             >  2:1076117/1‑101 (MQ=255)
                                                            tGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                         >  2:1904895/1‑101 (MQ=255)
                                                            tGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.AGCCCCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                         >  1:4157176/1‑101 (MQ=255)
                                                            tGGATCAAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                         <  1:829236/101‑1 (MQ=255)
                                                              gATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                       >  1:4855283/1‑101 (MQ=255)
                                                              gATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                       >  1:3286043/1‑101 (MQ=255)
                                                              gATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                       <  1:3221149/101‑1 (MQ=255)
                                                               agcGAAACAACCCGCTCCGGG.C.C..CGCCTCAGCGCCGCGCT.GC.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                      <  2:5000046/99‑1 (MQ=37)
                                                               aTCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                      >  1:2632627/1‑101 (MQ=255)
                                                                  gAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                                   >  2:3189930/1‑101 (MQ=255)
                                                                  gAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                                   >  2:1494845/1‑101 (MQ=255)
                                                                  gAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                                   <  1:1614023/101‑1 (MQ=255)
                                                                   aaccaccccGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                                  <  2:380915/95‑1 (MQ=255)
                                                                   aaaCAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                                  >  2:559564/1‑101 (MQ=255)
                                                                      caacCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.TGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc                               <  2:2937511/101‑1 (MQ=255)
                                                                             aTCAGGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                        <  1:1957493/101‑1 (MQ=255)
                                                                               cATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc                      >  1:3684807/1‑101 (MQ=255)
                                                                                aTGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACGTCCTCGGATCGGCCTGAGCCGCCCCACCCCTCgggctcggcc                     >  1:348363/1‑93 (MQ=25)
                                                                                aTGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc                     >  1:1867324/1‑101 (MQ=255)
                                                                                aTGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc                     >  1:3922569/1‑101 (MQ=255)
                                                                                aTGC.C.C..CGCCTCACCGCCGCCCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc                     <  2:4233367/101‑1 (MQ=255)
                                                                                  gg.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccccc                   >  2:1094282/1‑101 (MQ=255)
                                                                                   g.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc                  <  2:3371051/101‑1 (MQ=255)
                                                                                             ctgcgcgcCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg            <  2:2362878/97‑1 (MQ=255)
                                                                                                aGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCg         >  2:3096725/1‑101 (MQ=255)
                                                                                                  cgccgcGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTg       >  1:4195747/1‑101 (MQ=255)
                                                                                                   gccgcGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTgg      <  1:3102141/101‑1 (MQ=255)
                                                                                                    ccgcGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGa     <  2:2877416/101‑1 (MQ=255)
                                                                                                     cgcgCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGAt    <  1:4386526/101‑1 (MQ=255)
                                                                                                     cgcgCG.GA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGAt    <  1:224480/101‑1 (MQ=255)
                                                                                                      gcgcT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATc   <  1:3997690/101‑1 (MQ=255)
                                                                                                      gcgcT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATc   <  1:3056722/101‑1 (MQ=255)
                                                                                                      gcgcT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATc   <  1:2427696/101‑1 (MQ=255)
                                                                                                       cgcT.GA.CGTCGCTCCCCCGGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCg  <  2:1775403/101‑1 (MQ=255)
                                                                                                       cgcT.GA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCg  <  1:3747813/101‑1 (MQ=255)
                                                                                                       |                                                                                                      
GAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGG.C.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1397461‑1397660

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG