Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,668 A→G 2.2% Q517R (CAG→CGT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,6680A→G 2.2% 2.4 ‑1.0 ‑0.1225Q517R (CAG→CGG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (111/109):  total (112/113)
Fisher's exact test strand distribution p-value = 3.69e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.72e-01

Only 100 of 241 total aligned reads displayed.

TCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTC  >  NC_014034/1397568‑1397768
                                                                                                    |                                                                                                      
 cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACag                                                                                                       >  1:815834/1‑101 (MQ=255)
 cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACag                                                                                                       <  2:1600097/101‑1 (MQ=255)
 cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACag                                                                                                       >  2:2720073/1‑101 (MQ=255)
    tCCCCGCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGt                                                                                                    <  2:4737254/101‑1 (MQ=255)
      cccccCCGGGGGCGTGGAGCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTc                                                                                                  <  1:637085/101‑1 (MQ=255)
       ccccGCGGGGGCGTGGACCGAACCATCGGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTc.c                                                                                                <  2:1879505/101‑1 (MQ=37)
        cccGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.Cg                                                                                               <  2:3842903/101‑1 (MQ=255)
          cgcgGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGt                                                                                             <  2:4693619/101‑1 (MQ=255)
          cgcgGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGt                                                                                             <  2:3285781/101‑1 (MQ=255)
           gcgGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTc                                                                                            >  1:212996/1‑101 (MQ=255)
            cgGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCt                                                                                           >  2:4103809/1‑101 (MQ=255)
             gggggCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCtt                                                                                          >  1:4663298/1‑101 (MQ=255)
             gggggCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCtt                                                                                          <  1:1076230/101‑1 (MQ=255)
                 gCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgttc                                                                                      <  1:3486189/101‑1 (MQ=255)
                  ggTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgttct                                                                                     <  2:3438945/100‑1 (MQ=255)
                  cGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgttct                                                                                     >  1:3412172/1‑101 (MQ=255)
                  cGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgttct                                                                                     <  2:3570487/101‑1 (MQ=255)
                   gTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgttctg                                                                                    >  2:2866080/1‑101 (MQ=255)
                     ggATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgttctgtt                                                                                  >  1:4471175/1‑101 (MQ=255)
                      gATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgttctgttc                                                                                 >  2:4595442/1‑101 (MQ=255)
                      gATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTtgttctgttc                                                                                 <  2:4827671/101‑1 (MQ=255)
                           ttccgatctcgGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTa                                                                           <  2:202051/92‑1 (MQ=38)
                           aaaCATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTa                                                                           <  1:222531/101‑1 (MQ=255)
                           aaaCATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTa                                                                           >  2:2584909/1‑101 (MQ=255)
                            accctCCGCGGCTCGGCGGGGCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTaa                                                                          <  1:77229/97‑1 (MQ=37)
                            aaCATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTNTTGTTCTGTNN.Cgnnnn                                                                          >  2:1850527/1‑97 (MQ=255)
                             aCATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAAc                                                                         <  1:1494975/101‑1 (MQ=255)
                             aCATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAAc                                                                         <  1:4265640/101‑1 (MQ=255)
                             aCATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAAc                                                                         <  2:1166895/101‑1 (MQ=255)
                             aCATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAAc                                                                         <  2:539793/101‑1 (MQ=255)
                             aCATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAAc                                                                         <  1:3150430/101‑1 (MQ=255)
                             aCATCCGCGGATCGGCGCGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAAc                                                                         <  2:4546609/101‑1 (MQ=255)
                              cATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACa                                                                        >  1:3894691/1‑101 (MQ=255)
                                  cgcgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTc                                                                    <  1:1229291/101‑1 (MQ=255)
                                    cgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGc                                                                  <  2:4998902/101‑1 (MQ=255)
                                     ggATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCt                                                                 >  2:3978047/1‑101 (MQ=255)
                                       aTCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTcc                                                               >  2:3455468/1‑101 (MQ=255)
                                          ggCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTccccc                                                            <  2:3143598/101‑1 (MQ=255)
                                          ggCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTccccc                                                            >  2:2597590/1‑101 (MQ=255)
                                              tGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCcgcg                                                        >  1:1977587/1‑101 (MQ=255)
                                               gACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCgg                                                       >  1:3598992/1‑101 (MQ=255)
                                                  ccgccccgccCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCggggg                                                    <  1:2050508/101‑3 (MQ=255)
                                                   cgccccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGgggc                                                   >  1:1778951/1‑101 (MQ=255)
                                                    gccccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGggcg                                                  >  2:1739325/1‑101 (MQ=255)
                                                    gccccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGggcg                                                  >  1:3430979/1‑101 (MQ=255)
                                                     ccccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGt                                                 >  2:2221204/1‑101 (MQ=255)
                                                      cccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTTTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTg                                                >  1:2290058/1‑101 (MQ=255)
                                                      cccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGNGCGTg                                                >  1:1000238/1‑101 (MQ=255)
                                                       ccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTgg                                               >  2:108605/1‑101 (MQ=255)
                                                       ccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTgg                                               <  1:4050089/101‑1 (MQ=255)
                                                          cccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  1:2173160/101‑1 (MQ=255)
                                                           ccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                           >  1:2648977/1‑101 (MQ=255)
                                                           ccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  2:1992227/101‑1 (MQ=255)
                                                             aTCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         <  2:1231209/101‑1 (MQ=255)
                                                              tCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        >  2:1693434/1‑101 (MQ=255)
                                                                ggTCGCTCCCCCCGTGGGGGCGTGGATAGAAACTACATAGTTC.CGCTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                      <  2:2490752/101‑1 (MQ=37)
                                                                ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                      >  2:3664076/1‑101 (MQ=255)
                                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACtt                                     >  2:1329215/1‑101 (MQ=255)
                                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGt                                   <  1:335001/101‑1 (MQ=255)
                                                                     cTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CAGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCt                                 <  2:4157297/101‑1 (MQ=255)
                                                                      tCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTg                                >  2:3116734/1‑101 (MQ=255)
                                                                       ccccccGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGa                               <  1:3953712/101‑1 (MQ=255)
                                                                       ccccccGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGa                               >  1:4400558/1‑101 (MQ=255)
                                                                          cccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGGTTGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCtatggc                            >  1:1338110/1‑96 (MQ=0)
                                                                           ccGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGAcgcg                           <  1:505148/101‑1 (MQ=255)
                                                                           ccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGGTTGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCtatggcg                           <  1:2924162/101‑7 (MQ=0)
                                                                            cgcgGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACgcgc                          <  1:2781863/101‑1 (MQ=255)
                                                                            cgcgGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACgcgc                          >  1:1421842/1‑101 (MQ=255)
                                                                             gcgGGGGCGTGGATCGAAACGGCGGAGATCTCGGTCTGGGTGCGCTCGCGGTTGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCtatggcgga                         <  1:3673701/101‑9 (MQ=0)
                                                                               ngnngNNTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcn                       <  1:1850660/100‑2 (MQ=255)
                                                                               gggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcc                       >  1:22345/1‑100 (MQ=255)
                                                                               gggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                       >  2:4335759/1‑101 (MQ=255)
                                                                               gggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCACCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                       >  1:4106228/1‑101 (MQ=255)
                                                                                ggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcat                      <  2:4967708/101‑1 (MQ=255)
                                                                                ggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcat                      <  1:4595764/101‑1 (MQ=255)
                                                                                ggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcat                      <  2:2002437/101‑1 (MQ=255)
                                                                                 ggnCTTGGATCGAAACTNCAGAGTTC.CGGTCTTGTTCTGTTC.CGGTACCAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                     <  2:1000128/101‑1 (MQ=255)
                                                                                 gggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                     <  2:2381454/101‑1 (MQ=255)
                                                                                     gTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCa                 <  1:1739455/101‑1 (MQ=255)
                                                                                     gTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCa                 >  2:1029228/1‑101 (MQ=255)
                                                                                      tGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCAc                <  2:4662975/101‑1 (MQ=255)
                                                                                      tGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCAc                >  2:664010/1‑101 (MQ=255)
                                                                                       ggATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCGCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg               >  1:2573573/1‑101 (MQ=255)
                                                                                       ggATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg               >  2:4348069/1‑101 (MQ=255)
                                                                                       ggATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg               >  2:4769965/1‑101 (MQ=255)
                                                                                       ggATCGAAACGACAGAGTTC.CGGTCTTGTTCTGTGC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg               <  2:4264287/101‑1 (MQ=255)
                                                                                        gATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGt              <  2:2892965/101‑1 (MQ=255)
                                                                                         aTCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTc             <  2:729019/101‑1 (MQ=255)
                                                                                            gAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAg          <  2:1656463/101‑1 (MQ=255)
                                                                                            gAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAg          <  1:587567/101‑1 (MQ=255)
                                                                                             aaaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa         >  1:3565070/1‑101 (MQ=255)
                                                                                             aaaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa         >  2:2483789/1‑101 (MQ=255)
                                                                                             aaaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa         >  1:4893761/1‑101 (MQ=255)
                                                                                             aaaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa         >  1:1169007/1‑101 (MQ=255)
                                                                                             aaaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa         >  1:846906/1‑101 (MQ=255)
                                                                                                cTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTc      <  2:2522704/101‑1 (MQ=255)
                                                                                                 tACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCa     >  2:149178/1‑101 (MQ=255)
                                                                                                   cAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGt   >  2:1398491/1‑101 (MQ=255)
                                                                                                   cAGAGTTC.CGGTCTTGGTCTGCTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGt   <  1:2486692/101‑1 (MQ=255)
                                                                                                    agagTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTc  <  1:1099327/101‑1 (MQ=255)
                                                                                                    |                                                                                                      
TCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTC  >  NC_014034/1397568‑1397768

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG