Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,446,527 C→T 4.8% A142A (GCC→GCT RCAP_rcc01343 → iron siderophore/cobalamin ABC transporter permease

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,446,5270C→T 4.8% 17.2 0.0 ‑0.4186A142A (GCC→GCTRCAP_rcc01343iron siderophore/cobalamin ABC transporter permease
Reads supporting (aligned to +/- strand):  new base (4/5):  ref base (88/89):  total (92/94)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.93e-01

Only 100 of 238 total aligned reads displayed.

TCTCGGCGGGGGCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCTTTTCAACGCGCTGA  >  NC_014034/1446427‑1446627
                                                                                                    |                                                                                                    
 ctcGGCGGGGGCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTTTCCATGGGGGCTTTCGCGGGcgccttcgccg                                                                                                     <  1:4479721/101‑1 (MQ=255)
 ctcGGCGGGGGCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGcgccttcgccg                                                                                                     >  1:4694308/1‑101 (MQ=255)
   cggcggGGGCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGGGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGcgccttcgccgcc                                                                                                   >  2:3806932/1‑101 (MQ=255)
    ggcggGGGCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGcgccttcgccgcct                                                                                                  >  2:247036/1‑101 (MQ=255)
     gcggGGGCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGcgccttcgccgcctt                                                                                                 <  2:3406977/101‑1 (MQ=255)
      cggGGGCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGcgacttcgccgccttc                                                                                                <  1:3447995/101‑1 (MQ=255)
        ggggCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGcgccttcgccgccttcgc                                                                                              >  1:750821/1‑101 (MQ=255)
        ggggCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGcgccttcgccgccttcgc                                                                                              <  2:148319/101‑1 (MQ=255)
        ggggCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGcgccttcgccgccttcgc                                                                                              <  2:229289/101‑1 (MQ=255)
        ggggCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGcgccttcgccgccttcgc                                                                                              >  2:1903831/1‑101 (MQ=255)
          ggCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCgcgc                                                                                            <  2:2617316/101‑1 (MQ=255)
           gCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCt                                                                                           <  1:1668840/101‑1 (MQ=255)
              tCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCtggt                                                                                        >  2:3922975/1‑101 (MQ=255)
               cGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCtggtg                                                                                       >  2:1998721/1‑101 (MQ=255)
               cGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCtggtg                                                                                       <  1:1818334/101‑1 (MQ=255)
                gACCGGCGCGGTGGCGGTCATCGTGCTGGGCAGCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCtgttgg                                                                                      <  2:4277372/101‑1 (MQ=255)
                 aCCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGTCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGgc                                                                                     <  2:346701/101‑1 (MQ=255)
                     gcgcGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCtt                                                                                 <  2:2831111/101‑1 (MQ=255)
                       gcgGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCTCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCtttt                                                                               <  1:1792468/101‑1 (MQ=255)
                       gcgGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCtttt                                                                               >  2:695969/1‑101 (MQ=255)
                        cgGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTg                                                                              >  2:320656/1‑101 (MQ=255)
                         ggTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGt                                                                             <  1:4885511/101‑1 (MQ=255)
                         ggTGGCGGTCATCGTGCTGGGCATAGGCGCGGGCGAGCTGTCGCTGTTCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCTCTTTTGt                                                                             <  2:3693051/101‑1 (MQ=14)
                           gggCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCg                                                                           <  2:2092176/100‑1 (MQ=255)
                             gCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGaa                                                                         <  2:4943720/101‑1 (MQ=255)
                             gCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGaa                                                                         >  2:4556602/1‑101 (MQ=255)
                              cGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAAc                                                                        >  1:3304116/1‑101 (MQ=255)
                              cGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAAc                                                                        <  1:2494564/101‑1 (MQ=255)
                               ggTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACg                                                                       >  2:3291268/1‑101 (MQ=255)
                               ggTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACg                                                                       >  1:4254384/1‑101 (MQ=255)
                                gTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACgg                                                                      >  2:6541/1‑101 (MQ=255)
                                gTCATCGTGCTGGGAATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACgg                                                                      >  1:820863/1‑101 (MQ=255)
                                 tCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGgc                                                                     >  1:1526079/1‑101 (MQ=255)
                                 tCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGgc                                                                     >  2:2998880/1‑101 (MQ=255)
                                  cATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGgcg                                                                    <  2:1742286/101‑1 (MQ=255)
                                  cATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGgcg                                                                    >  2:1280235/1‑101 (MQ=255)
                                  cATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGgcg                                                                    >  2:3083469/1‑101 (MQ=255)
                                     cGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGa                                                                 >  1:4168777/1‑101 (MQ=255)
                                     cGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGa                                                                 >  2:3890580/1‑101 (MQ=255)
                                      gTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGAc                                                                >  2:1138225/1‑101 (MQ=255)
                                      gTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGAc                                                                >  1:402483/1‑101 (MQ=255)
                                      gTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGAc                                                                <  1:4783930/101‑1 (MQ=255)
                                      gTGCTGGGCATCGGCGCGGGAGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGAc                                                                >  1:430523/1‑101 (MQ=255)
                                       tGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGAcc                                                               <  1:5041869/101‑1 (MQ=255)
                                       tGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGAcc                                                               >  2:3137756/1‑101 (MQ=255)
                                        gCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCa                                                              <  2:4012138/101‑1 (MQ=255)
                                           gggCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCg                                                           <  1:558752/101‑1 (MQ=255)
                                           gggCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCg                                                           >  2:4929202/1‑101 (MQ=255)
                                             gCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCggg                                                         >  1:2245819/1‑101 (MQ=255)
                                                tCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCg                                                      <  2:901388/101‑1 (MQ=255)
                                                tCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCg                                                      <  1:2401107/101‑1 (MQ=255)
                                                  ggCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGaa                                                    <  1:3660470/101‑1 (MQ=255)
                                                   gcgcGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAAc                                                   <  2:3493665/101‑1 (MQ=40)
                                                     gcgggcggGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACca                                                 >  1:3727745/1‑101 (MQ=39)
                                                      cgggcgggCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGGCTTCGCTGCCTTCGCGTTTTTCGGGCTTTTGTCGCACTGTGCGACCAGCGGGCCGAAccgc                                                >  2:5018229/1‑99 (MQ=11)
                                                      cgggcgggCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACcac                                                >  1:1106536/1‑101 (MQ=38)
                                                      cgggcgggCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACcac                                                <  1:1595702/101‑1 (MQ=38)
                                                       gggcgggcTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACcaca                                               >  2:2252212/1‑101 (MQ=38)
                                                         gcgggcTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCTGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACg                                             <  2:4825625/101‑1 (MQ=14)
                                                         gcgggcTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACg                                             >  1:3488612/1‑101 (MQ=38)
                                                         gcgggcTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACg                                             <  2:2386851/101‑1 (MQ=38)
                                                             gctgtcgctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATcc                                         <  1:293716/101‑1 (MQ=38)
                                                              ctgtcgctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCt                                        <  1:3249228/101‑1 (MQ=38)
                                                               tgtcgctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTc                                       <  2:3734928/101‑1 (MQ=38)
                                                               tgtcgctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTc                                       >  2:747869/1‑101 (MQ=38)
                                                               tgtcgctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTc                                       >  1:3190810/1‑101 (MQ=38)
                                                                gtcgctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCTGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTcg                                      <  1:4143843/101‑1 (MQ=14)
                                                                 tcgctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCTGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTcgc                                     >  2:4374302/1‑101 (MQ=14)
                                                                 tcgctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTcgc                                     <  1:2999108/101‑1 (MQ=38)
                                                                   gctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCTGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCgg                                   >  2:1023400/1‑101 (MQ=14)
                                                                   gctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACAAGATcggaagag                                   >  1:3602830/1‑94 (MQ=255)
                                                                    ctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCggg                                  >  1:497606/1‑101 (MQ=37)
                                                                     tgtcCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCgggg                                 >  1:877612/1‑101 (MQ=35)
                                                                        ccATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTc                              <  2:3320185/101‑1 (MQ=32)
                                                                         cATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTcg                             <  2:3514977/101‑1 (MQ=32)
                                                                         cATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTcg                             >  2:1395733/1‑101 (MQ=32)
                                                                          aTGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTcgc                            <  2:4871907/101‑1 (MQ=32)
                                                                            gggggCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTcgccg                          >  2:4995518/1‑101 (MQ=33)
                                                                            gggggCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTcgccg                          <  1:2690398/101‑1 (MQ=32)
                                                                            gggggCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTcgccg                          <  1:4921896/101‑1 (MQ=32)
                                                                              gggCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCgccgcc                        >  1:3962973/1‑101 (MQ=33)
                                                                                gCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTc                      <  2:3375454/101‑1 (MQ=32)
                                                                                gCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTc                      <  1:3936998/101‑1 (MQ=32)
                                                                                  tttCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGc                    <  2:2922194/101‑1 (MQ=32)
                                                                                  tttCGCGGGCGCCTTCGCCGCCATCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGACCCTCGCGGGGGTCGCCTCCTCGc                    <  2:820761/101‑1 (MQ=255)
                                                                                   ttCGGGGGCGCCGTCGCCGCCTTCGCGCTGGTGGCGCTTTGGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCa                   <  1:1069434/101‑1 (MQ=255)
                                                                                   ttCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCa                   <  1:598251/101‑1 (MQ=32)
                                                                                    tCGCGGGCGCCTGCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAg                  >  1:2741029/1‑101 (MQ=18)
                                                                                     cgcgGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGc                 <  2:2558802/101‑1 (MQ=32)
                                                                                       cgGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGGCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCtt               <  1:1964779/101‑1 (MQ=17)
                                                                                        gggCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCttt              <  1:696058/101‑1 (MQ=32)
                                                                                        gggCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCttt              >  2:4973151/1‑101 (MQ=32)
                                                                                          gcgcCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCTTTTc            >  1:1661409/1‑101 (MQ=32)
                                                                                          gcgcCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCTTTTc            <  1:1280356/101‑1 (MQ=32)
                                                                                           cgccttcgccgccttcgcGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCTTTTCa           <  2:4622681/101‑1 (MQ=32)
                                                                                              cttcgccgccttcgcGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCTTTTCAAcg        >  1:3099971/1‑101 (MQ=32)
                                                                                              cttcgccgccttcgcGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCTTTTCAAcg        >  1:1379199/1‑101 (MQ=32)
                                                                                               ttcgccgccttcgcGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCTTTTCAAcgc       >  2:478035/1‑101 (MQ=32)
                                                                                                 cgccgccttcgcGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCTTTTCAACgcgc     >  2:1971892/1‑101 (MQ=32)
                                                                                                   ccgccttcgcGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCTTTTCAACGCGCTg   >  2:2057699/1‑101 (MQ=31)
                                                                                                    |                                                                                                    
TCTCGGCGGGGGCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCTTTTCAACGCGCTGA  >  NC_014034/1446427‑1446627

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG