Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,669,016 T→C 100% K383E (AAG→GAG)  ilvI ← acetolactate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,669,0160T→C100.0% 918.4 240K383E (AAG→GAG) ilvIacetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (149/89):  ref base (0/0):  total (149/91)

Only 100 of 275 total aligned reads displayed.

GCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGCTGCGCCCCTTGGTCAGGGCCTTCAGCCGCTCCAGCGCATATTGCGGTTTGGTGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCCCACCAT  >  NC_014034/1668918‑1669113
                                                                                                  |                                                                                                 
gCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCa                                                                                                 <  1:4190338/101‑1 (MQ=255)
 cGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAg                                                                                                >  1:2417003/1‑101 (MQ=255)
  ggTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGc                                                                                               <  2:1136949/101‑1 (MQ=255)
  ggTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGc                                                                                               >  2:2547948/1‑101 (MQ=255)
    ttCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCg                                                                                             >  1:3986212/1‑101 (MQ=255)
    ttCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCg                                                                                             >  1:4115312/1‑101 (MQ=255)
     tCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCGGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGc                                                                                            >  2:2025761/1‑101 (MQ=255)
      cGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCt                                                                                           >  1:1283687/1‑101 (MQ=255)
       ggCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTc                                                                                          <  2:1189435/101‑1 (MQ=255)
       ggCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTc                                                                                          >  2:2490990/1‑101 (MQ=255)
        gccccTCGAAGCCCAGATATTGCGCCGCCCGCATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTcc                                                                                         <  1:2245817/98‑1 (MQ=255)
         cgcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCa                                                                                        <  2:2775285/101‑1 (MQ=255)
         cgcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCa                                                                                        <  2:4417527/101‑1 (MQ=255)
          gcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAg                                                                                       >  1:983033/1‑101 (MQ=255)
           ccTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgc                                                                                      <  1:2051862/101‑1 (MQ=255)
           ccTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgc                                                                                      >  2:3241505/1‑101 (MQ=255)
           ccTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgc                                                                                      >  2:2664655/1‑101 (MQ=255)
            cTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgcg                                                                                     >  2:214750/1‑101 (MQ=255)
            cTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgcg                                                                                     >  2:1865697/1‑101 (MQ=255)
             tCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgcgc                                                                                    >  1:2346959/1‑101 (MQ=255)
                aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCata                                                                                 >  1:3725967/1‑101 (MQ=255)
                aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCata                                                                                 >  2:3832159/1‑101 (MQ=255)
                aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCata                                                                                 >  1:460426/1‑101 (MQ=255)
                aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCata                                                                                 >  2:3018566/1‑101 (MQ=255)
                aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCata                                                                                 <  1:3241756/101‑1 (MQ=255)
                aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCata                                                                                 >  1:3393673/1‑101 (MQ=255)
                 aGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCatat                                                                                <  1:1162840/101‑1 (MQ=255)
                 aGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCatat                                                                                >  2:4936072/1‑101 (MQ=255)
                  gCCCAGATATTGCGCCGCCCACGTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATAtt                                                                               <  2:2353415/101‑1 (MQ=255)
                        atatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGtt                                                                         >  1:254018/1‑101 (MQ=255)
                         tatTGCGCCGCCCACATCTGATGCTGGCCGGCTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTACAGCCGCTCCAGCGCATATTGCGGttt                                                                        <  1:349588/101‑1 (MQ=255)
                         tatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGttt                                                                        <  2:1740017/101‑1 (MQ=255)
                         tatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGttt                                                                        <  2:3459743/101‑1 (MQ=255)
                          atTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTg                                                                       >  2:1160246/1‑101 (MQ=255)
                           ttGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTga                                                                      <  1:1124797/101‑2 (MQ=255)
                            tGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTgat                                                                     >  2:3300660/1‑99 (MQ=255)
                             gcgcCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATg                                                                    >  2:2753957/1‑101 (MQ=255)
                             gcgcCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATg                                                                    <  2:1790248/101‑1 (MQ=255)
                               gccgccCACATCTGATGCTGGCCGACTTCGGGGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGAt                                                                  >  1:5050962/1‑101 (MQ=255)
                                  gccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCt                                                               >  1:4591559/1‑101 (MQ=255)
                                    ccACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGt                                                             <  1:880765/101‑1 (MQ=255)
                                      ccaTCGGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCCCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTcc                                                           <  2:2282318/100‑1 (MQ=255)
                                      acaTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTcc                                                           <  1:72536/101‑1 (MQ=255)
                                       caTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCg                                                          >  1:1403753/1‑101 (MQ=255)
                                       caTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCg                                                          >  1:4963730/1‑101 (MQ=255)
                                        aTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGa                                                         <  1:2199079/101‑1 (MQ=255)
                                        aTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGa                                                         >  1:654404/1‑101 (MQ=255)
                                        aTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGa                                                         >  1:1640560/1‑101 (MQ=255)
                                        aTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGa                                                         <  2:3054753/101‑1 (MQ=255)
                                         tCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGCCCGAg                                                        >  1:2716249/1‑101 (MQ=255)
                                           tGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGcc                                                      >  2:4597922/1‑101 (MQ=255)
                                           tGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGcc                                                      >  2:809972/1‑101 (MQ=255)
                                            gATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTACAGCGCGTATTGCGGTTTGATGATGCTGTCCGGGCCg                                                     >  1:3024203/1‑101 (MQ=255)
                                              tGCGGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGgt                                                   <  2:5032548/101‑1 (MQ=255)
                                               gCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGgtg                                                  >  1:4452521/1‑101 (MQ=255)
                                                 tGGCCGACTTGGGGGGTGATGTAGCGGTTGCGCCCCTTGGGCGGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTa                                                <  1:3811133/101‑1 (MQ=255)
                                                  ggCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAg                                               >  2:2187312/1‑101 (MQ=255)
                                                     cGACTTTGGGGGTGATGTAGCGGTTGGCCCTCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCg                                            <  1:2013004/101‑1 (MQ=255)
                                                     cGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCg                                            >  1:4206735/1‑101 (MQ=255)
                                                       ccTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAg                                          <  1:3458672/100‑1 (MQ=255)
                                                           cGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAg                                      >  1:1107101/1‑101 (MQ=255)
                                                            ggtggtGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGc                                     >  1:1761119/1‑101 (MQ=255)
                                                            ggtggtGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGc                                     >  1:3045099/1‑101 (MQ=255)
                                                             gtggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCg                                    >  2:1594586/1‑101 (MQ=255)
                                                               ggtgATGTGGCGGTTGCGCCCCTTGGTCGGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGa                                  <  1:3582877/101‑1 (MQ=255)
                                                               ggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGa                                  >  1:4802972/1‑101 (MQ=255)
                                                                gtaaTGTGGGGGTTGGGCCCCTTGGCAAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGAGGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAt                                 <  1:4877528/98‑1 (MQ=255)
                                                                 tgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtc                                >  2:1083182/1‑101 (MQ=255)
                                                                  taTGTAGGGGTTCCGCCCCTTGGTCAGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGAGGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtct                               <  1:2563796/100‑1 (MQ=255)
                                                                  gaggTAGGGGTTGCCCCCTTTGGCCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtct                               <  2:4205389/98‑1 (MQ=255)
                                                                  gATGTAGGGGTTGCCCCCTTTGGTCGGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtct                               <  2:1334359/101‑1 (MQ=255)
                                                                  gATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtct                               >  1:2625291/1‑101 (MQ=255)
                                                                   ttGTGGCGGTTGCCCCCCTTGGTCAGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtctt                              <  1:4507663/100‑1 (MQ=255)
                                                                    tGTAGCGGTTGCGCCCCTTGGTCGGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttc                             <  1:2912543/101‑1 (MQ=255)
                                                                    tGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttc                             >  1:4848770/1‑101 (MQ=255)
                                                                     gTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttct                            >  1:1501072/1‑101 (MQ=255)
                                                                     gTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttct                            >  2:1477483/1‑101 (MQ=255)
                                                                     gTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttct                            >  2:1884633/1‑101 (MQ=255)
                                                                     gTAGCGGTTGCGCCCCTGGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttct                            <  2:4114995/101‑1 (MQ=255)
                                                                      ccgatcttGCGCCCCTTGGTCAGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATcttctt                           <  1:1371935/94‑1 (MQ=255)
                                                                       ggcgtgtccgcCCCTTGGTCGGGCCCCCCAGCCGCTCCAGCGCATATTGCGTTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCttcttc                          <  2:3711904/93‑1 (MQ=255)
                                                                       ggCGGTTGCCCCCCTTGGTCGGGGCCCCCAGCCTCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCttcttc                          <  2:4788955/100‑1 (MQ=255)
                                                                       aGCGGTTGCCCCCCTTGGTCAGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGCCCGAGCCGGTGTAGGCGAGGCAGCGGATCttcttc                          <  1:2759816/101‑1 (MQ=255)
                                                                        gcgttggcgcCCTTGGGTCGGGCCCTCCGGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTcc                         <  2:2646367/95‑1 (MQ=255)
                                                                           tttccgcCCTTTGTTCAGGGCCCCCAGCCGCTCCAGCGCATATTGGGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCAct                      <  2:4074128/97‑1 (MQ=255)
                                                                           gttGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGAGTAGGCGAGGCAGCGGATCTTCTTCCAct                      >  1:395128/3‑101 (MQ=255)
                                                                           ggtcccccccTTGGTCGGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCAct                      <  2:933516/95‑1 (MQ=255)
                                                                            ttGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActc                     >  1:463427/2‑101 (MQ=255)
                                                                             tGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcc                    >  1:696238/1‑101 (MQ=255)
                                                                              ggcccccTTGGCCGGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcct                   <  2:1403627/98‑1 (MQ=255)
                                                                              gcgcCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcct                   >  2:378315/1‑101 (MQ=255)
                                                                                gcCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCg                 >  2:4550681/1‑101 (MQ=255)
                                                                                cccccTGGGGCGGGGCCCCCAGCCGCTCCAGCGCATATTGGGGTTTGATGATGCTGTCGGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCg                 <  1:996216/100‑1 (MQ=255)
                                                                                   ccTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATc              >  1:3187777/1‑101 (MQ=255)
                                                                                     tgggTCGGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCtt            <  2:463362/99‑1 (MQ=255)
                                                                                      tGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTg           >  2:3597186/1‑101 (MQ=255)
                                                                                       ggTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGc          >  2:1658540/1‑101 (MQ=255)
                                                                                             ggCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCccacc    >  2:3914323/1‑101 (MQ=255)
                                                                                              gCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGGTCTTTCCccacca   >  1:4031365/1‑101 (MQ=255)
                                                                                               ccTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCCCACCAt  >  1:1913069/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGCTGCGCCCCTTGGTCAGGGCCTTCAGCCGCTCCAGCGCATATTGCGGTTTGGTGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCCCACCAT  >  NC_014034/1668918‑1669113

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG