Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,786,541 C→T 4.6% A225A (GCC→GCT RCAP_rcc01648 → ABC transporter permease

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,786,5410C→T 4.6% 17.0 0.0 ‑0.0219A225A (GCC→GCTRCAP_rcc01648ABC transporter permease
Reads supporting (aligned to +/- strand):  new base (5/5):  ref base (100/109):  total (105/114)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.39e-01

Only 100 of 235 total aligned reads displayed.

TGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCT.G.GCCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATCC  >  NC_014034/1786444‑1786641
                                                                                                   |                                                                                                    
tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGGCTtt                                                                                                    <  2:1845906/101‑1 (MQ=255)
tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGGCTtt                                                                                                    >  1:3797989/1‑101 (MQ=255)
tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGGCTtt                                                                                                    <  1:436619/101‑1 (MQ=255)
 gATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGGCTttc                                                                                                   <  2:2836708/101‑2 (MQ=255)
 gATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGGCTttc                                                                                                   <  2:3639314/101‑2 (MQ=255)
 gATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGGCTttc                                                                                                   <  2:3334522/101‑2 (MQ=255)
  aTGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCg                                                                                                 <  2:4830590/101‑1 (MQ=255)
   tGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGt                                                                                                <  1:369935/101‑1 (MQ=255)
   tGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGt                                                                                                >  2:369882/1‑101 (MQ=255)
   tGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGt                                                                                                <  1:176705/101‑1 (MQ=255)
    gCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTa                                                                                               <  1:2713517/101‑1 (MQ=255)
    gCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTa                                                                                               <  1:1313669/101‑1 (MQ=255)
       gCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATcc                                                                                            >  2:4456478/1‑101 (MQ=255)
        cATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCa                                                                                           >  2:3141339/1‑101 (MQ=255)
         aTCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCTAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCAt                                                                                          <  2:1641175/101‑1 (MQ=255)
         aTCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCAt                                                                                          <  2:3174414/101‑1 (MQ=255)
         aTCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCAt                                                                                          >  2:5003705/1‑101 (MQ=255)
         aTCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCAt                                                                                          >  1:4087972/1‑101 (MQ=255)
         aTCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCAt                                                                                          >  2:738966/1‑101 (MQ=255)
           cTGATCGCCGCGGGGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCt                                                                                        <  2:2294276/101‑1 (MQ=255)
           cTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCt                                                                                        <  2:1233707/101‑1 (MQ=255)
            tGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTg                                                                                       >  2:3107189/1‑101 (MQ=255)
            tGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTg                                                                                       <  1:4717661/101‑1 (MQ=255)
             gATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGa                                                                                      >  1:2015276/1‑101 (MQ=255)
             gATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGa                                                                                      >  2:4793990/1‑101 (MQ=255)
             gATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGa                                                                                      >  1:2533221/1‑101 (MQ=255)
             gATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGa                                                                                      <  2:4068038/101‑1 (MQ=255)
              aTCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGaa                                                                                     <  1:1003901/101‑1 (MQ=255)
                cgccgcGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAAtt                                                                                   <  1:411300/101‑1 (MQ=255)
                cgccgcGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAAtt                                                                                   <  2:1279095/101‑1 (MQ=255)
                  ccgcGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTc                                                                                 <  1:3953767/101‑1 (MQ=255)
                   cgcggcggggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTTGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTcc                                                                                <  1:2857546/101‑1 (MQ=255)
                   cgcggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGTTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTcc                                                                                >  2:2484475/1‑101 (MQ=255)
                   cgcggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTcc                                                                                <  2:2605587/101‑1 (MQ=255)
                   cgcggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTcc                                                                                <  1:97518/101‑1 (MQ=255)
                     cggggcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAg                                                                              <  1:3895681/101‑1 (MQ=255)
                     cggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAg                                                                              <  1:2303899/101‑1 (MQ=255)
                      ggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGa                                                                             >  1:1055865/1‑101 (MQ=255)
                        cgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATg                                                                           >  1:3313336/1‑101 (MQ=255)
                        cgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATg                                                                           <  1:3049631/101‑1 (MQ=255)
                         gcgggcggGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGt                                                                          >  2:1540122/1‑101 (MQ=255)
                           gggcggggCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTc                                                                        <  1:4608310/101‑1 (MQ=255)
                            ggcgggggTTTGGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                       <  2:2351348/101‑1 (MQ=255)
                            ggcggggcTTTCGCTGATCTGGAAGGTTGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                       <  1:1651440/101‑1 (MQ=255)
                            ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                       >  2:4594607/1‑101 (MQ=255)
                            ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                       <  1:2158096/101‑1 (MQ=255)
                              gggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                     <  2:1983440/95‑1 (MQ=255)
                                ggactttCGATGATCTGGAAGGTGGTGCTGGTGAGCAAATTTCT.GCGCCGCTCGAACGCGATCGG.CTTTCGTTTCCATCTGAATTTCCAGATGTTCCACAt                                                                   <  2:561922/97‑1 (MQ=255)
                                 ggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAtt                                                                  <  2:2051430/98‑1 (MQ=255)
                                 ggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATNTCCAGATGTTCGACAtt                                                                  >  2:1598670/4‑101 (MQ=255)
                                  gctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTg                                                                 >  1:3668835/3‑101 (MQ=255)
                                   ctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGc                                                                >  1:1778805/2‑101 (MQ=255)
                                   ctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGc                                                                >  1:1952965/2‑101 (MQ=255)
                                    tttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGcc                                                               >  1:949614/1‑101 (MQ=255)
                                    tttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGcc                                                               <  1:447094/101‑1 (MQ=255)
                                      tCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGa                                                             >  2:4671590/1‑101 (MQ=255)
                                      cttccgATCT.GAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAg                                                            <  2:1673418/96‑1 (MQ=255)
                                        gCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAgg                                                           >  1:4987096/1‑101 (MQ=255)
                                                ggAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGcc                                                   <  1:5024105/101‑1 (MQ=255)
                                                 gAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAgagatcggaag                                                  >  1:1673547/1‑91 (MQ=255)
                                                 gAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCt                                                  <  2:4898790/101‑1 (MQ=255)
                                                  aaGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTg                                                 <  2:3733531/101‑1 (MQ=255)
                                                   aagTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTgg                                                <  1:2484649/99‑1 (MQ=255)
                                                       cGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcgg                                            >  2:1059326/1‑101 (MQ=255)
                                                        gTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                           >  1:226365/1‑101 (MQ=255)
                                                        gTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                           <  2:148989/101‑1 (MQ=255)
                                                        gTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                           >  2:1272604/1‑101 (MQ=255)
                                                           cTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggcggc                                        >  2:3956887/1‑101 (MQ=255)
                                                               tggtCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTc                                    <  1:31464/101‑1 (MQ=255)
                                                               tggtCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTc                                    <  2:3324941/101‑1 (MQ=255)
                                                                 gtCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGt                                  >  2:3547741/1‑101 (MQ=255)
                                                                   cGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCt                                >  2:4528610/1‑101 (MQ=255)
                                                                    gAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTg                               >  1:2130711/1‑101 (MQ=255)
                                                                      aTTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCg                             >  1:1678616/1‑101 (MQ=255)
                                                                        ttctgg.gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTg                           >  2:2799744/6‑101 (MQ=255)
                                                                         tctgg.gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGa                          >  2:9245/5‑101 (MQ=255)
                                                                         tctgg.gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGa                          >  2:2340809/5‑101 (MQ=255)
                                                                         tctgg.gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGa                          <  1:821553/97‑1 (MQ=255)
                                                                           tgg.gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                        <  2:3878235/99‑1 (MQ=255)
                                                                            gg.gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGc                       >  2:3428567/2‑101 (MQ=255)
                                                                            gg.gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGc                       >  2:2715854/2‑101 (MQ=255)
                                                                                 cGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGc                   >  2:2109112/1‑101 (MQ=255)
                                                                                    tCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGt                <  1:4538025/101‑1 (MQ=255)
                                                                                     cGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTc               >  1:1546048/1‑101 (MQ=255)
                                                                                     cGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTc               <  1:1598802/101‑1 (MQ=255)
                                                                                     cGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTc               <  2:1768062/101‑1 (MQ=255)
                                                                                     cGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTc               <  2:4286199/101‑1 (MQ=255)
                                                                                     cGAACGGGATCGG.CTTTCGCATCCATCTGATTTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTc               <  1:331574/101‑1 (MQ=255)
                                                                                      gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg              >  2:2192574/1‑101 (MQ=255)
                                                                                      gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg              >  1:4224315/1‑101 (MQ=255)
                                                                                      gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg              >  1:654137/1‑101 (MQ=255)
                                                                                          gggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATg          <  2:3046315/101‑1 (MQ=255)
                                                                                           ggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATgg         >  2:3806781/1‑101 (MQ=255)
                                                                                            gATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGc        <  1:4782559/101‑1 (MQ=255)
                                                                                             aTCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCt       <  1:3451962/101‑1 (MQ=255)
                                                                                             aTCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCt       <  2:1092679/101‑1 (MQ=255)
                                                                                                cggcTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGa     <  2:2533040/98‑1 (MQ=255)
                                                                                                  gcTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATc   >  2:4380968/2‑101 (MQ=255)
                                                                                                  gcTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATc   <  1:265736/100‑1 (MQ=255)
                                                                                                   cTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATcc  >  1:1093919/1‑101 (MQ=255)
                                                                                                   |                                                                                                    
TGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCT.G.GCCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATCC  >  NC_014034/1786444‑1786641

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG