Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,794,302 G→C 90.9% A398A (GCG→GGC)  exaA2 → quinoprotein ethanol dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,794,3020G→C90.9% 43.9 ‑3.0 0.0209A398A (GCG→GCC) exaA2quinoprotein ethanol dehydrogenase
Reads supporting (aligned to +/- strand):  new base (98/92):  ref base (5/14):  total (103/106)
Fisher's exact test strand distribution p-value = 5.23e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

AGAACATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GCGGCCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGGCTCTTCTAT  >  NC_014034/1794208‑1794395
                                                                                               |                                                                                             
agaaCATTACCTGAGCCAAGGGCATCTATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGACCCCGCGCCCCCGATGCCGCGCCCGAA.CCCGGCAAg                                                                                         >  2:1040932/1‑101 (MQ=255)
 gaaCATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAgg                                                                                        <  2:4599405/101‑1 (MQ=255)
  aaCATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAggg                                                                                       >  1:3161105/1‑101 (MQ=255)
   aCATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGc                                                                                      <  1:333915/101‑1 (MQ=255)
    cATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGcc                                                                                     <  2:328824/101‑1 (MQ=255)
    cATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGcc                                                                                     <  2:3842322/101‑1 (MQ=255)
      tCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCcag                                                                                   <  2:4058921/101‑1 (MQ=255)
      tCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCcag                                                                                   >  1:771344/1‑101 (MQ=255)
      tCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCcag                                                                                   >  1:3269094/1‑101 (MQ=255)
       cACCTGGGCCAAGGGCATCGATGAAGCCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCcagc                                                                                  <  2:2619742/101‑1 (MQ=255)
         ccTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCcagcag                                                                                >  1:4149379/1‑101 (MQ=255)
          cTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGa                                                                               >  1:1635621/1‑101 (MQ=255)
           tGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGAt                                                                              >  1:4687284/1‑101 (MQ=255)
            gggCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATc                                                                             >  2:1298066/1‑101 (MQ=255)
              gCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCtt                                                                           >  1:3691833/1‑101 (MQ=255)
                cAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTg                                                                         <  2:1012507/101‑1 (MQ=255)
                 aaGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGc                                                                        >  2:1372090/1‑101 (MQ=255)
                 aaGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGc                                                                        >  1:2565291/1‑101 (MQ=255)
                  aGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGcc                                                                       <  2:2896165/101‑1 (MQ=255)
                    ggCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGt                                                                     >  1:1961357/1‑101 (MQ=255)
                    ggCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGt                                                                     <  1:3552593/101‑1 (MQ=255)
                     gCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTg                                                                    <  2:2567599/101‑1 (MQ=255)
                     gCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTg                                                                    <  1:704565/101‑1 (MQ=255)
                          gATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTc                                                               <  2:529555/101‑1 (MQ=255)
                          gATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTc                                                               >  1:4838131/1‑101 (MQ=255)
                          gATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTc                                                               >  1:89504/1‑101 (MQ=255)
                           aTGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCg                                                              >  2:2359701/1‑101 (MQ=255)
                              aaaCCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTc                                                           >  2:2084737/1‑101 (MQ=255)
                               aaCCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTcc                                                          <  1:25200/101‑1 (MQ=255)
                                aCCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGTTGCCCCCGATGCCGCGGCCGAA.GGCGGCAACGGCCAGCAGATCTTTGCCCTCCCCTCGTTCCt                                                         >  1:3612034/1‑101 (MQ=255)
                                 ccGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTg                                                        >  2:2515212/1‑101 (MQ=255)
                                   ggCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTggg                                                      >  1:4326822/1‑101 (MQ=255)
                                   ggCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGCTCCTggg                                                      >  2:616501/1‑101 (MQ=255)
                                    gCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggc                                                     <  2:641028/101‑1 (MQ=255)
                                     ccGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggcg                                                    >  1:935648/1‑101 (MQ=255)
                                       gTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGCA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggcggc                                                  >  1:1177106/1‑101 (MQ=255)
                                       gTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggcggc                                                  <  2:3641167/101‑1 (MQ=255)
                                        tCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCa                                                 <  1:3871287/101‑1 (MQ=255)
                                        tCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCa                                                 <  1:1361556/101‑1 (MQ=255)
                                         ccGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCaa                                                >  2:4235042/1‑101 (MQ=255)
                                          cGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAg                                               <  1:1854715/101‑1 (MQ=255)
                                           gATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGa                                              <  2:1608645/101‑1 (MQ=255)
                                           gATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGa                                              >  1:2167741/1‑101 (MQ=255)
                                            aTCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGaa                                             <  1:3418304/101‑1 (MQ=255)
                                             tCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAAc                                            >  2:1550051/1‑101 (MQ=255)
                                              cTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACt                                           >  2:2114373/1‑101 (MQ=255)
                                              cTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACt                                           >  1:2385307/1‑101 (MQ=255)
                                               tACAGCGAGGAGACCCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTg                                          <  2:1956692/101‑1 (MQ=255)
                                                aCAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTgg                                         <  2:581750/101‑1 (MQ=255)
                                                aCAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTgg                                         >  1:45198/1‑101 (MQ=255)
                                                 cAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGa                                        <  1:1395169/101‑1 (MQ=255)
                                                     gaggagAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGcc                                    >  2:1682385/1‑101 (MQ=255)
                                                      aggagAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCg                                   >  1:2752914/1‑101 (MQ=255)
                                                        gagaACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGAt                                 >  2:731454/1‑101 (MQ=255)
                                                         agaACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATg                                >  2:4253861/1‑101 (MQ=255)
                                                         agaACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATg                                >  1:4958523/1‑101 (MQ=255)
                                                          gaACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATgg                               >  2:4823464/1‑101 (MQ=255)
                                                          gaACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATgg                               <  1:616580/101‑1 (MQ=255)
                                                           aaCCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGc                              >  2:3582558/1‑101 (MQ=255)
                                                            aCCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGcc                             <  1:4077746/101‑1 (MQ=255)
                                                             ccGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCt                            <  2:4349340/101‑1 (MQ=255)
                                                             ccGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCt                            <  2:3321569/101‑1 (MQ=255)
                                                             ccGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCAGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCt                            <  2:2208977/101‑1 (MQ=255)
                                                              cGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTa                           <  1:2048788/101‑1 (MQ=255)
                                                               gCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTAc                          >  2:1459491/1‑101 (MQ=255)
                                                               gCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTAc                          >  2:1820000/1‑101 (MQ=255)
                                                                 cccGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAg                        <  1:4018549/101‑1 (MQ=255)
                                                                 cccGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAg                        <  1:533847/101‑1 (MQ=255)
                                                                 cccGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCATCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAg                        >  2:5030549/1‑101 (MQ=38)
                                                                   cGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGcc                      <  1:4987734/101‑1 (MQ=255)
                                                                   cGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGcc                      <  2:175536/101‑1 (MQ=255)
                                                                   cGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGcc                      <  2:4200866/101‑1 (MQ=255)
                                                                   cGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGcc                      >  1:3480957/1‑101 (MQ=255)
                                                                   cGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGcc                      <  2:1430874/101‑1 (MQ=255)
                                                                    ggTGCCCCCGATGCGGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCa                     <  1:4801731/101‑1 (MQ=255)
                                                                     gTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAg                    <  2:4008572/101‑1 (MQ=255)
                                                                     gTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAg                    >  2:3269781/1‑101 (MQ=255)
                                                                      tGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGa                   <  1:4904376/101‑1 (MQ=255)
                                                                       gCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGAc                  >  2:2938602/1‑101 (MQ=255)
                                                                        cccccGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGAcc                 <  2:4875705/101‑1 (MQ=255)
                                                                         ccccGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCa                <  1:4855392/101‑1 (MQ=255)
                                                                          cccGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCTTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCAc               <  2:4372616/101‑1 (MQ=255)
                                                                          cccGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCAc               <  2:1278797/101‑1 (MQ=255)
                                                                           ccGATGCCGCGGCCGAA.GGCGGCAAGTGCCAGCAGATCTTTGCCGCGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACg              <  1:4243873/101‑1 (MQ=38)
                                                                            cGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACgg             >  2:3049467/1‑101 (MQ=255)
                                                                            cGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACgg             <  2:2776390/101‑1 (MQ=255)
                                                                            cGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACgg             <  1:2550711/101‑1 (MQ=255)
                                                                             gATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACggg            >  1:2733521/1‑101 (MQ=255)
                                                                             gATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACggg            >  2:4033125/1‑101 (MQ=255)
                                                                             gATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACggg            <  2:1884015/101‑1 (MQ=255)
                                                                              aTGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACgggc           >  2:3155263/1‑100 (MQ=38)
                                                                              aTGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACgggc           >  1:1221421/1‑100 (MQ=255)
                                                                                gCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCct         >  1:503956/1‑101 (MQ=37)
                                                                                gCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCct         <  2:4326505/101‑1 (MQ=255)
                                                                                gCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCct         <  2:4502582/101‑1 (MQ=255)
                                                                                gCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCGTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCct         <  1:4372974/101‑1 (MQ=255)
                                                                                    cgGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCtcttc     <  1:45640/101‑1 (MQ=38)
                                                                                     ggCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCtcttct    >  1:4431658/1‑101 (MQ=255)
                                                                                     ggCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCtcttct    <  1:1298187/101‑1 (MQ=38)
                                                                                       ccGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCTCTTCTAt  <  2:242065/101‑1 (MQ=38)
                                                                                               |                                                                                             
AGAACATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GCGGCCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGGCTCTTCTAT  >  NC_014034/1794208‑1794395

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG