Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,965,271	C→G	100%	intergenic (+39/+53)	bdhA → / ← RCAP_rcc01818	3‑hydroxybutyrate dehydrogenase/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,965,271	0	C→G	100.0%	36.3	13	intergenic (+39/+53)	bdhA/RCAP_rcc01818	3‑hydroxybutyrate dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/11):  ref base (0/0):  total (2/11)

GGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GCGGG..CT.CCG.CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTCT  >  NC_014034/1965175‑1965367                                                                                                    |                                                                                                     ggcggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GCgggg                                                                                                  >  1:1154506/1‑100 (MQ=255)    ggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGgggggcc                                                                                               >  1:2286917/1‑97 (MQ=255)                            tggggtggggggcggcGGGCGGGCGCGGGGAGAGTCGCCGCCCGGGGGCACCCCCGCGCCCCCCCG.CC..GGGGG..CT.CCGCCCCCGGACCCCCGAGGTATTTc                                                                    <  2:1356168/91‑1 (MQ=0)                              cTGTGGATGGCGGCTGGACGGCGCTGGGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCGCCC..GGGGGC.CC.CCC.CCCCGGCCCCCCGCGG.ATTTCAg                                                                  >  2:3390175/1‑101 (MQ=16)                                      ggcggcTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CCCGGGGGG..GC.CCC.CCCCGCCCCCCCCCGGCGTTTCAGGCAAggt                                                           >  2:4771822/1‑99 (MQ=12)                                               aCGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CCGGGGGGG..CC.CCC.CCCCCGACCCCCCCGGGGTTTCAGGCAAGAGGAAAAAggg                                                  >  2:4826728/1‑101 (MQ=16)                                                         gagagaTTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGG..CTCCCC.CCCCCGCCCCCCCCGGGGTTTCTGGGAAGATGAGAAGGGGGGGCCCAggcg                                       >  2:3000679/1‑101 (MQ=2)                                                              aTTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGGCCC.CCC.CCCCCCCCCCCCCGGGGGTTTAAGGCAAAAGAAAAAAGGGGGGCGCAGGGGGccc                                   >  2:1419160/1‑100 (MQ=0)                                                                       cGGGGCAAGCCCGAGGCGACCCG.CCG.GGGGG..CC.CCC.CCCCCGGCCCCCCAGGGATTTCAGGGCAGAGGAAAAAGGGGGGGCCCGGCGGCCCTCTTcgcccc                         >  1:655753/1‑99 (MQ=12)                                                                               gCCCGGGCCGCCCCG.CC..GGGGG..CT.CCGCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCg                 <  1:3794863/101‑1 (MQ=25)                                                                                  gggggccccccG.CC..GGGGG..CT.CCGCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGgtg              <  2:2050063/93‑1 (MQ=25)                                                                                  gctctccccccG.CC..GGGGG..CT.CCGCCCCCGGTCCCCCGAGGTATTTCAGTCAAGATGAAAGAGGGTCGCTCAGGCGGCCTTTTTCGCCGCCTTGCCCGgtg              <  2:4937263/93‑1 (MQ=21)                                                                                  gcacaacccccG.CC..GGGGG..CT.CCGCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGgtg              <  1:1709446/93‑1 (MQ=25)                                                                                     ccccccccG.CC..GGGGG..CT.CCGCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGtct           <  1:4711328/96‑1 (MQ=25)                                                                                      cccccccG.CC..GGGGG..CT.CCGCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGtctc          <  2:3226265/97‑1 (MQ=25)                                                                                      cccccccG.CC..GGGGG..CT.CCGCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGtctc          <  2:2913735/97‑1 (MQ=25)                                                                                        cccccG.CC..GGGGG..CT.CCGCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCgg        <  1:3272171/99‑1 (MQ=255)                                                                                         ccccG.CC..GGGGG..CT.CCGCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCggg       <  2:154660/100‑1 (MQ=255)                                                                                         ccccG.CC..GGGGG..CT.CCGCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCggg       <  1:720428/100‑1 (MQ=255)                                                                                         ccccG.CC..GGGGG..CT.CCGCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCggg       <  1:2170517/100‑1 (MQ=255)                                                                                          cccG.CC..GGGGG..CT.CCGCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGc      <  2:1731684/101‑1 (MQ=255)                                                                                          cccG.CC..GGGGG..CT.CCGCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGc      <  1:4772145/101‑1 (MQ=255)                                                                                            cg.cc..ggGGG..CT.CCGCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGctt    <  1:4440118/101‑1 (MQ=255)                                                                                             g.cc..ggGGG..CT.CCGCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGcttc   <  2:4473384/101‑1 (MQ=255)                                                                                               cc..ggGGG..CT.CCGCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGcttct  <  1:1602031/101‑1 (MQ=255)                                                                                                    |                                                                                                     GGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GCGGG..CT.CCG.CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTTCT  >  NC_014034/1965175‑1965367 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG