Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,057,834 A→C 17.9% intergenic (‑600/‑302) RCAP_rcc01900 ← / → RCAP_rcc01901 hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,057,8340A→C17.9% 92.1 ‑0.2 ‑0.4184intergenic (‑600/‑302)RCAP_rcc01900/RCAP_rcc01901hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (18/15):  ref base (85/66):  total (103/81)
Fisher's exact test strand distribution p-value = 8.49e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.15e-01

Only 100 of 190 total aligned reads displayed.

CTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CCGCG.CG.C.AGC.G.TTCCGAACCGGCATCAATCTTGG.C.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057735‑2057913
                                                                                                        |                                                                                   
cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CG.C.CGc                                                                                   <  2:3537309/101‑1 (MQ=255)
cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CG.C.CGc                                                                                   >  1:1743170/1‑101 (MQ=255)
 tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CG.C.CGca                                                                                  >  2:2912967/1‑100 (MQ=255)
  gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CG.C.CGcaa                                                                                 >  2:2387183/1‑99 (MQ=255)
  gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CG.C.CGcaa                                                                                 >  1:2180755/1‑99 (MQ=255)
    ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CG.C.CGcaagc                                                                               >  2:2359337/1‑97 (MQ=255)
     tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CG.C.CGcaagcg                                                                              >  1:2305349/1‑96 (MQ=255)
     tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCCGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CG.C.CGcaagcg                                                                              >  2:3240113/1‑96 (MQ=255)
      aCCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CG.C.CGcaagcgt                                                                             >  1:1978745/1‑95 (MQ=255)
        cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CG.C.CGcaagcgttt                                                                           <  1:938795/101‑9 (MQ=255)
        cGCCCATTAACCCTGCTACGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CG.C.CGcaagcgttt                                                                           <  2:4537891/101‑9 (MQ=255)
         gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CG.C.CGcaagcgtttc                                                                          <  1:868832/101‑10 (MQ=255)
         gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CG.C.CGcaagcgtttc                                                                          <  1:3117434/101‑10 (MQ=255)
          cccATTAACCCTGCTTCGGCGCGCGGTCTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CG.C.CGcaagcgtttcc                                                                         <  2:77477/101‑11 (MQ=255)
          cccATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CG.C.CGcaagcgtttcc                                                                         >  2:4536369/1‑91 (MQ=255)
          cccATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CG.C.CGcaagcgtttcc                                                                         <  1:3675029/101‑11 (MQ=255)
          cccATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CG.C.CGcaagcgtttcc                                                                         <  1:1130168/101‑11 (MQ=255)
                aaCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCg                                                                      >  2:559884/1‑101 (MQ=255)
                aaCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCg                                                                      >  1:2723847/1‑101 (MQ=255)
                 aCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCgg                                                                     <  1:1103324/101‑1 (MQ=255)
                   ccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCa                                                                   >  2:1089473/1‑101 (MQ=255)
                    cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCAt                                                                  <  2:1498896/101‑1 (MQ=255)
                     tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATc                                                                 <  2:1276859/101‑1 (MQ=255)
                      gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCa                                                                >  1:4004313/1‑101 (MQ=255)
                      gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCa                                                                >  2:2096574/1‑101 (MQ=255)
                      gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCa                                                                <  2:4836371/101‑1 (MQ=255)
                      gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCa                                                                >  2:4712298/1‑101 (MQ=255)
                      gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCa                                                                >  2:3416095/1‑101 (MQ=255)
                      gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCa                                                                >  2:4043085/1‑101 (MQ=255)
                         tCGGCGCGCGGACTTCGGGGCGGCGGCTGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTc                                                             <  2:1069589/101‑1 (MQ=255)
                         tCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTc                                                             >  1:2329137/1‑101 (MQ=255)
                          cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCt                                                            <  2:2341171/101‑1 (MQ=255)
                          cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCt                                                            >  2:1877895/1‑101 (MQ=255)
                           ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCtt                                                           >  2:1233990/1‑101 (MQ=255)
                              gcgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGG.c                                                       <  1:2506478/101‑1 (MQ=255)
                                  ggACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGG.c.cccc                                                  <  2:51737/101‑1 (MQ=255)
                                  ggACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGG.c.cccc                                                  >  1:1888698/1‑101 (MQ=255)
                                   gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGG.c.ccccc                                                 <  1:1004193/101‑2 (MQ=255)
                                   gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGG.c.ccccc                                                 <  1:3613280/101‑2 (MQ=255)
                                     cTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGCGG.TTCCGAACCGGCATCATTCTTGG.CGCCccgc                                                <  1:4569932/101‑1 (MQ=255)
                                     cTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGG.c.cccccgc                                               <  1:2924385/101‑4 (MQ=255)
                                     cTTCGGGGCGGCGGCGGGGCCCTTGCGATGCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGG.CGCCccgc                                                >  2:2145241/1‑101 (MQ=255)
                                      ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGG.c.cccccgcc                                              >  2:1921167/1‑97 (MQ=255)
                                      ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGG.c.cccccgcc                                              <  2:2817483/101‑5 (MQ=255)
                                      ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGG.c.cccccgcc                                              <  1:3218845/101‑5 (MQ=255)
                                       tCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGG.c.cccccgccg                                             >  1:588749/1‑96 (MQ=255)
                                        cGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCC.AGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCgg                                             >  1:1256150/1‑101 (MQ=255)
                                        cGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCgg                                             >  1:1582550/1‑101 (MQ=255)
                                        cGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCgg                                             >  2:2225902/1‑101 (MQ=255)
                                        cGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCgg                                             >  2:372791/1‑101 (MQ=255)
                                          gggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCgggc                                           >  2:1970415/1‑100 (MQ=255)
                                           ggcggcggcGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGAc                                          >  1:4057807/1‑101 (MQ=255)
                                           ggcggcggcGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GCTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGAc                                          <  1:5003782/101‑1 (MQ=255)
                                            gcggcggcgGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACa                                         <  1:2387347/101‑1 (MQ=255)
                                               gcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATc                                      <  2:1173766/101‑1 (MQ=255)
                                                cggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCa                                     <  2:2535850/101‑1 (MQ=255)
                                                cggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCa                                     >  1:3570170/1‑101 (MQ=255)
                                                   cggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACgg                                  >  2:1855093/1‑101 (MQ=255)
                                                   cggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACgg                                  <  1:4864594/101‑1 (MQ=255)
                                                    ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGgc                                 >  2:4284863/1‑101 (MQ=255)
                                                        ctctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGgccgcc                             >  2:1022823/1‑101 (MQ=255)
                                                         tctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCt                            >  2:5003515/1‑101 (MQ=255)
                                                          ctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTc                           >  1:1292088/1‑101 (MQ=255)
                                                          ctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTc                           >  2:951152/1‑101 (MQ=255)
                                                          ctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTc                           >  1:3812158/1‑101 (MQ=255)
                                                           ttGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCa                          >  1:4844565/1‑101 (MQ=255)
                                                              cGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTg                       >  2:4329392/1‑101 (MQ=255)
                                                              cGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAACC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTg                       >  1:4218018/1‑101 (MQ=255)
                                                                aTCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGtt                     <  2:4026067/101‑1 (MQ=255)
                                                                  ccTGCGTTTCGGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTcc                   <  2:3959044/101‑1 (MQ=255)
                                                                  ccTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTcc                   <  1:1691237/101‑1 (MQ=255)
                                                                  ccTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTcc                   >  2:2572864/1‑101 (MQ=255)
                                                                  ccTGCATTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTcc                   <  2:4125183/101‑1 (MQ=255)
                                                                   cTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCg                  >  1:4793304/1‑101 (MQ=255)
                                                                     gCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcc                >  1:4921444/1‑101 (MQ=255)
                                                                     gCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcc                >  2:3115425/1‑101 (MQ=255)
                                                                     gCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcc                >  2:1693895/1‑101 (MQ=255)
                                                                     gCGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcc                >  2:1581085/1‑101 (MQ=255)
                                                                      cGTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCa               >  1:393009/1‑101 (MQ=255)
                                                                       gTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt              >  1:1066522/1‑101 (MQ=255)
                                                                       gTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt              >  2:4068372/1‑101 (MQ=255)
                                                                       gTTTCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt              >  2:4207468/1‑101 (MQ=255)
                                                                        tttCCGGATTGGCGACAA.TCCCGCGCCG.CAAGCTT.TTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             <  1:3115663/101‑1 (MQ=255)
                                                                         ttCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTg            >  2:4897028/1‑101 (MQ=255)
                                                                          tCCGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGc           >  2:1757056/1‑101 (MQ=255)
                                                                           ccGGATTGGCGACAACC.CCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCg          <  1:548916/101‑1 (MQ=255)
                                                                           ccGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCg          <  1:695505/101‑1 (MQ=255)
                                                                            cGGATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCCTTGTTCCGCCATTGCgc         >  1:3515438/1‑100 (MQ=255)
                                                                             ggATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  1:224227/1‑101 (MQ=255)
                                                                             ggATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  2:267249/1‑101 (MQ=255)
                                                                              gATTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc       <  1:4408685/101‑1 (MQ=255)
                                                                               aTTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      <  1:3324047/101‑1 (MQ=255)
                                                                               aTTGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      >  1:526629/1‑101 (MQ=255)
                                                                                ttGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     <  1:1616499/101‑1 (MQ=255)
                                                                                 tGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATGCCGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    >  1:4336451/1‑101 (MQ=255)
                                                                                 tGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    >  2:1553451/1‑101 (MQ=255)
                                                                                 tGGCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    >  2:1344710/1‑101 (MQ=255)
                                                                                  ggCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGNCGCCTCATTGTTCCGCCATTGCGATCACAg   >  1:764887/1‑101 (MQ=255)
                                                                                  ggCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAg   <  2:1978601/101‑1 (MQ=255)
                                                                                   gCGACAA.TCCCGCGCCG.CAAGC.GTTTCCGAACCGGCATCATTCTTGGCC.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGc  <  1:3761281/101‑1 (MQ=255)
                                                                                                        |                                                                                   
CTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CCGCG.CG.C.AGC.G.TTCCGAACCGGCATCAATCTTGG.C.CCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057735‑2057913

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG