Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,123,416 T→C 100% Q252Q (CAA→CAG wza ← polysaccharide export protein Wza

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,123,4160T→C100.0% 616.8 162Q252Q (CAA→CAGwzapolysaccharide export protein Wza
Reads supporting (aligned to +/- strand):  new base (88/74):  ref base (0/0):  total (88/74)

Only 100 of 168 total aligned reads displayed.

CTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAAGGGCTTGA.C.ATTTGGGCCAACAAATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCT  >  NC_014034/2123326‑2123507
                                                                                            |                                                                                           
tttGCCCTCGGCAGGACCGCCGGCGGGGTGGTTCGCCTGGGTCGGGGTGATGTTTTCCAGCGGCAGATGGATGGTCG.GGGGCTTGA.C.GTTCTGGcccagca                                                                                  <  2:2624266/100‑6 (MQ=255)
    tCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGTGCTTGA.CGATCTGGCCCAGCAGATa                                                                               <  2:4384425/101‑1 (MQ=255)
    tCCTCGGCCAGACCGCCGACGCGGGGGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATa                                                                               >  2:2036247/1‑101 (MQ=255)
     ccTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAg                                                                              <  2:3874116/101‑1 (MQ=255)
      cTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAgg                                                                             >  2:854200/1‑101 (MQ=255)
       tCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGc                                                                            >  2:4344391/1‑101 (MQ=255)
        cGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcc                                                                           <  2:412507/101‑1 (MQ=255)
          gCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCGAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGGTCTGGCCCAGCAGATAGGcctc                                                                         >  2:4721562/1‑101 (MQ=255)
          gCCAGACCGCCAACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcctc                                                                         >  1:2647290/1‑101 (MQ=255)
           ccAGACCGCCGACGCGGGTGATCGCCTTGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcctcc                                                                        <  2:707654/101‑1 (MQ=255)
            cAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcctcct                                                                       >  1:4923592/1‑101 (MQ=255)
            cAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcctcct                                                                       <  1:4849442/101‑1 (MQ=255)
             aGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTg                                                                      >  1:1774298/1‑101 (MQ=255)
             aGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTg                                                                      >  1:2772391/1‑101 (MQ=255)
             aGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTg                                                                      >  1:2717134/1‑101 (MQ=255)
              gACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGc                                                                     <  1:4949642/101‑1 (MQ=255)
               aCCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCa                                                                    >  2:1633953/1‑101 (MQ=255)
                ccgccgACGCGGGTGATAGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCAc                                                                   <  2:4544360/101‑1 (MQ=255)
                 cgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCAcc                                                                  <  2:3297563/101‑1 (MQ=255)
                 cgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCAcc                                                                  <  2:1500109/101‑1 (MQ=255)
                 cgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCAcc                                                                  <  2:1469530/101‑1 (MQ=255)
                   ccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGt                                                                <  2:4601235/101‑1 (MQ=255)
                    cgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGT.GGAGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTc                                                               <  1:4734585/101‑1 (MQ=255)
                      aCGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTg                                                             >  2:1248220/1‑101 (MQ=255)
                        gcgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgcc                                                           >  2:4093460/1‑101 (MQ=255)
                         cgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgccg                                                          >  1:3552557/1‑101 (MQ=255)
                         cgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgccg                                                          >  1:5041236/1‑101 (MQ=255)
                         cgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgccg                                                          <  1:3979757/101‑1 (MQ=255)
                           ggTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgccgcc                                                        <  2:2184579/101‑1 (MQ=255)
                             tGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCg                                                      >  1:3721795/1‑101 (MQ=255)
                              gATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGGCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCgc                                                     >  2:2359013/1‑100 (MQ=255)
                               aTCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGAc                                                    <  1:854302/101‑1 (MQ=255)
                                tCGCCTGGGTCAGGGTGATGTTTTCCCGCGTCCGATCGATCGTCG.AGGGCTTGACC.ATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACg                                                   <  2:4938301/101‑1 (MQ=255)
                                 cGCCTGTGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGc                                                  <  2:4707615/101‑1 (MQ=255)
                                  gCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCt                                                 <  1:1806270/101‑1 (MQ=255)
                                   ccTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTg                                                <  2:1415824/101‑1 (MQ=255)
                                   ccTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTg                                                <  1:4104651/101‑1 (MQ=255)
                                   ccTGGGTCAGGGTGATGTTTTCCAGCCTCAGATCGATCGTCG.AGGGTTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTg                                                <  1:2002470/101‑1 (MQ=255)
                                     tGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgac                                              <  1:2975893/101‑1 (MQ=255)
                                      gggTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacg                                             >  1:4600612/1‑101 (MQ=255)
                                      gggTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacg                                             <  2:375549/101‑1 (MQ=255)
                                        gTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacgac                                           >  1:17862/1‑101 (MQ=255)
                                        gTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacgac                                           <  2:2997807/101‑1 (MQ=255)
                                         tCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACa                                          >  2:3424485/1‑101 (MQ=255)
                                         tCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACa                                          <  2:2726881/101‑1 (MQ=255)
                                          cAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACAt                                         >  1:1004959/1‑101 (MQ=255)
                                           aGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATc                                        <  1:2067261/101‑1 (MQ=255)
                                           aGGGGGATGTTTTCCAGCGTCAGATCGATCGGCG.AGGGCTTGA.CGATCTGGCCCAGGAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATc                                        <  2:1508979/101‑1 (MQ=255)
                                              gTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGcc                                     >  2:4456571/1‑101 (MQ=255)
                                              gTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGcc                                     <  2:4270695/101‑1 (MQ=255)
                                               tGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCg                                    <  1:791443/101‑1 (MQ=255)
                                               tGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCg                                    >  2:4055845/1‑101 (MQ=255)
                                                gATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGt                                   >  1:4933073/1‑101 (MQ=255)
                                                gATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGt                                   >  1:4465034/1‑101 (MQ=255)
                                                 aTGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtt                                  <  1:511175/101‑1 (MQ=255)
                                                  tGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGttt                                 <  1:1486933/101‑1 (MQ=255)
                                                  tGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGttt                                 <  1:2164775/101‑1 (MQ=255)
                                                   gTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtttt                                >  1:2910063/1‑101 (MQ=255)
                                                   gTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtttt                                <  2:2498708/101‑1 (MQ=255)
                                                   gTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtttt                                >  1:1046799/1‑101 (MQ=255)
                                                   gTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtttt                                <  2:3119564/101‑1 (MQ=255)
                                                    ttttCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTg                               <  1:240652/101‑1 (MQ=255)
                                                    ttttCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTg                               >  1:4480180/1‑101 (MQ=255)
                                                    ttttCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTg                               >  1:1419724/1‑101 (MQ=255)
                                                     tttCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCCCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgc                              <  1:2036391/101‑1 (MQ=255)
                                                      ttCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgca                             >  1:2810996/1‑101 (MQ=255)
                                                      ttCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgca                             >  1:3711152/1‑101 (MQ=255)
                                                      ttCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgca                             >  1:3909821/1‑101 (MQ=255)
                                                       tcaaGCGTCAGATCGATCGTAG.AGGGCTTGA.CGATCTGGACCAGCAGATAGGCCTCCTGCACCGACTGCCGACCGACGCCGACGACATAGACGTTTTgcag                            >  2:800310/4‑101 (MQ=255)
                                                       tCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcag                            <  2:2675791/101‑1 (MQ=255)
                                                       tCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcag                            >  2:1121721/1‑101 (MQ=255)
                                                        ccAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcagc                           <  2:1064479/101‑1 (MQ=255)
                                                         cAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcagca                          >  1:2992891/1‑101 (MQ=255)
                                                          aGCGTCAGATCGATCGTCG.ACGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCAc                         >  2:338633/1‑101 (MQ=255)
                                                             gTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCgg                      >  1:2492129/1‑101 (MQ=255)
                                                               cAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgt                    >  2:1840555/1‑101 (MQ=255)
                                                                aGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGGCATCGCCGTTTTGCAGCACCGGgtt                   <  1:3168816/101‑1 (MQ=255)
                                                                aGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgtt                   >  2:2759389/1‑101 (MQ=255)
                                                                aGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgtt                   >  2:1520084/1‑101 (MQ=255)
                                                                 gatcgatcGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttg                  >  2:2644098/1‑101 (MQ=255)
                                                                 gatcgatcGTCG.AGGGCTTAA.CGATCTGGCCCAGCAGGTAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttg                  >  1:3339590/1‑101 (MQ=255)
                                                                   tcgatcgTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttgtt                <  1:4740717/101‑1 (MQ=255)
                                                                     gatcgTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg              >  1:4113446/1‑101 (MQ=255)
                                                                        cgtcg.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgcgc           >  1:3086504/1‑101 (MQ=255)
                                                                        cgtcg.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgcgc           <  1:1327270/101‑1 (MQ=255)
                                                                           tcgaGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCg         <  2:4932743/98‑1 (MQ=255)
                                                                            cgaGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGa        >  1:3834023/3‑101 (MQ=255)
                                                                            cgaGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGa        >  1:23263/3‑101 (MQ=255)
                                                                            cgaGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGa        >  2:2582512/3‑101 (MQ=255)
                                                                            cgaGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGa        >  2:3344658/3‑101 (MQ=255)
                                                                            cgaGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGa        >  2:4765287/3‑101 (MQ=255)
                                                                            cgaGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGa        <  2:4860013/99‑1 (MQ=255)
                                                                             gaGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGAt       <  2:968088/100‑1 (MQ=255)
                                                                             gaGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGAt       >  2:994159/2‑101 (MQ=255)
                                                                             gaGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGAt       >  2:2847918/2‑101 (MQ=255)
                                                                             gaGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCGCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGAt       <  1:4301938/100‑1 (MQ=255)
                                                                              aGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGGGCCGATg      >  1:3840837/1‑101 (MQ=255)
                                                                              aGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATg      >  1:2652926/1‑101 (MQ=255)
                                                                                  cTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCt  >  1:3359911/1‑101 (MQ=255)
                                                                                  cTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCt  <  2:2911710/101‑1 (MQ=255)
                                                                                            |                                                                                           
CTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAAGGGCTTGA.C.ATTTGGGCCAACAAATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCT  >  NC_014034/2123326‑2123507

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG