Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,123,427 A→G 100% M249M (TTG→CTG)  wza ← polysaccharide export protein Wza

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,123,4270A→G100.0% 768.2 200M249M (TTG→CTG) wzapolysaccharide export protein Wza
Reads supporting (aligned to +/- strand):  new base (104/96):  ref base (0/0):  total (104/96)

Only 100 of 206 total aligned reads displayed.

TCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAAGGGCTTGA.C.ATTTGGGCCAACAAATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGAAAGGC  >  NC_014034/2123330‑2123520
                                                                                                   |                                                                                             
tCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGTGCTTGA.CGATCTGGCCCAGCAGATa                                                                                            <  2:4384425/101‑1 (MQ=255)
 gcTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAg                                                                                           <  1:807091/100‑1 (MQ=255)
    cGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcc                                                                                        >  2:4091925/1‑101 (MQ=255)
     ggCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGCTAGGcct                                                                                       >  1:2766243/1‑101 (MQ=255)
      gCCAGACCGCCAACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcctc                                                                                      >  1:2647290/1‑101 (MQ=255)
       ccAGACCGCCGACGCGGGTGATCGCCTTGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGcctcc                                                                                     <  2:707654/101‑1 (MQ=255)
         aGACCGCCGTCGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGACCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTg                                                                                   <  2:4798324/101‑1 (MQ=255)
         aGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTg                                                                                   <  1:1298790/101‑1 (MQ=255)
         aGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTg                                                                                   >  1:2717134/1‑101 (MQ=255)
         aGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGAAGATAGGCCTCCTg                                                                                   >  2:4877735/1‑101 (MQ=255)
          gACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGc                                                                                  <  1:4949642/101‑1 (MQ=255)
           aCCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCa                                                                                 >  2:888840/1‑101 (MQ=255)
           aCCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCa                                                                                 >  1:2010604/1‑101 (MQ=255)
            ccgccgACGCGGGTGATAGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCAc                                                                                <  2:4544360/101‑1 (MQ=255)
             cgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCAcc                                                                               <  2:1500109/101‑1 (MQ=255)
             cgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCAcc                                                                               >  1:2911936/1‑101 (MQ=255)
              gccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCg                                                                              >  2:391004/1‑101 (MQ=255)
                cgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGT.GGAGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTc                                                                            <  1:4734585/101‑1 (MQ=255)
                  aCGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTg                                                                          <  2:2520282/101‑1 (MQ=255)
                      gggTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgccgc                                                                      >  2:2829071/1‑101 (MQ=255)
                        gTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGccc                                                                    >  1:4417640/1‑101 (MQ=255)
                         tGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCg                                                                   <  2:2666665/101‑1 (MQ=255)
                          gATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGa                                                                  >  2:1736801/1‑101 (MQ=255)
                          gATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGGCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCgc                                                                  >  2:2359013/1‑100 (MQ=255)
                           aTCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGAc                                                                 >  1:4565767/1‑101 (MQ=255)
                            tCGCCTGGGTCAGGGTGATGTTTTCCCGCGTCCGATCGATCGTCG.AGGGCTTGACC.ATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACg                                                                <  2:4938301/101‑1 (MQ=255)
                            tCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACg                                                                >  1:3639817/1‑101 (MQ=255)
                             cGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGc                                                               >  2:4301621/1‑101 (MQ=255)
                              gCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGCCGCt                                                              >  2:985012/1‑101 (MQ=255)
                              gCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCt                                                              <  1:1806270/101‑1 (MQ=255)
                               ccTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTg                                                             <  2:1415824/101‑1 (MQ=255)
                               ccTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTg                                                             <  1:4104651/101‑1 (MQ=255)
                                 tGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgac                                                           <  1:5033094/101‑1 (MQ=255)
                                  gggTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacg                                                          <  2:375549/101‑1 (MQ=255)
                                   ggTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacga                                                         >  2:4708517/1‑101 (MQ=255)
                                    gTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacgac                                                        <  1:4869629/101‑1 (MQ=255)
                                      cAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACAt                                                      >  1:1004959/1‑101 (MQ=255)
                                       aGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATc                                                     <  1:2067261/101‑1 (MQ=255)
                                       aGGGGGATGTTTTCCAGCGTCAGATCGATCGGCG.AGGGCTTGA.CGATCTGGCCCAGGAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATc                                                     <  2:1508979/101‑1 (MQ=255)
                                        gggTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCg                                                    >  1:4125782/1‑101 (MQ=255)
                                        gggTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCg                                                    <  2:3408186/101‑1 (MQ=255)
                                          gTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGcc                                                  <  2:1624779/101‑1 (MQ=255)
                                          gTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGcc                                                  <  2:4228681/101‑1 (MQ=255)
                                            gATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGt                                                >  1:4933073/1‑101 (MQ=255)
                                            gATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGt                                                >  1:4465034/1‑101 (MQ=255)
                                             aTGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtt                                               <  1:511175/101‑1 (MQ=255)
                                              tGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGttt                                              <  2:2680191/101‑1 (MQ=255)
                                              tGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGttt                                              <  1:1486933/101‑1 (MQ=255)
                                              tGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGttt                                              <  1:2164775/101‑1 (MQ=255)
                                                ttttCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTg                                            <  1:240652/101‑1 (MQ=255)
                                                 tttCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgc                                           >  1:876376/1‑101 (MQ=255)
                                                 tttCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGAAGCTGACGACATCGCCGGTTTgc                                           >  2:4670398/1‑101 (MQ=255)
                                                  ttCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgca                                          >  1:2810996/1‑101 (MQ=255)
                                                   tcaaGCGTCAGATCGATCGTAG.AGGGCTTGA.CGATCTGGACCAGCAGATAGGCCTCCTGCACCGACTGCCGACCGACGCCGACGACATAGACGTTTTgcag                                         >  2:800310/4‑101 (MQ=255)
                                                   tCCAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcag                                         >  2:1121721/1‑101 (MQ=255)
                                                     cAGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcagca                                       >  1:2992891/1‑101 (MQ=255)
                                                      aGCGTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCAc                                      <  1:3956540/101‑1 (MQ=255)
                                                         gTCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCgg                                   >  1:2492129/1‑101 (MQ=255)
                                                          tCAGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCggg                                  >  2:4462896/1‑101 (MQ=255)
                                                            aGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGGCATCGCCGTTTTGCAGCACCGGgtt                                <  1:3168816/101‑1 (MQ=255)
                                                            aGATCGATCGTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgtt                                >  2:2759389/1‑101 (MQ=255)
                                                             gatcgatcGTCG.AGGGCTTAA.CGATCTGGCCCAGCAGGTAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttg                               >  1:3339590/1‑101 (MQ=255)
                                                                 gatcgTCG.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg                           >  2:205040/1‑101 (MQ=255)
                                                                    cgtcg.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgcgc                        <  1:1327270/101‑1 (MQ=255)
                                                                    cgtcg.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgcgc                        >  1:3086504/1‑101 (MQ=255)
                                                                    cgtcg.AGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgcgc                        >  2:3344284/1‑101 (MQ=255)
                                                                       tcgaGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTNNNNCNg                      >  1:714466/4‑101 (MQ=255)
                                                                       tcgaGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCg                      >  2:3253608/4‑101 (MQ=255)
                                                                       tcgaGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCg                      >  2:2870642/4‑101 (MQ=255)
                                                                        cgaGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGa                     >  1:3834023/3‑101 (MQ=255)
                                                                        cgaGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGa                     >  2:2582512/3‑101 (MQ=255)
                                                                         gaGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGAt                    >  1:1786052/2‑101 (MQ=255)
                                                                         gaGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCGCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGAt                    <  1:4301938/100‑1 (MQ=255)
                                                                          aGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGGGCCGATg                   >  1:3840837/1‑101 (MQ=255)
                                                                          aGGGCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATg                   >  1:1368684/1‑101 (MQ=255)
                                                                            ggCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGcc                 >  1:3733070/1‑101 (MQ=255)
                                                                             gCTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCTCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCTCCGATGccc                <  2:3104163/101‑1 (MQ=255)
                                                                              cTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCt               >  1:3359911/1‑101 (MQ=255)
                                                                              cTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCt               <  2:2911710/101‑1 (MQ=255)
                                                                              cTTGA.CGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCt               <  1:4759502/101‑1 (MQ=255)
                                                                                ttgacgatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTg              <  2:3856121/92‑1 (MQ=255)
                                                                                ttgacgatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTg              <  1:4251462/92‑1 (MQ=255)
                                                                                 tgacgatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCGGCACCGGGTTGTTGGCGCCGATGCCCTGt             <  2:1307869/93‑1 (MQ=255)
                                                                                 tgacgatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGt             <  1:1904833/93‑1 (MQ=255)
                                                                                  gacgatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGtt            <  1:2845220/94‑1 (MQ=255)
                                                                                   acgatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTc           <  2:2010460/95‑1 (MQ=255)
                                                                                    cgatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCTTGTTcc          >  1:1696952/6‑101 (MQ=255)
                                                                                    cgatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTcc          <  2:1240095/96‑1 (MQ=255)
                                                                                      atctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGCCGCTGACGACATCGCCGTTTTGCAGCACCCGGTTGTTGGCGCCGATGCCCTGTTCCAg        >  2:4747773/4‑101 (MQ=255)
                                                                                      atctGGCCCAGCAGATAGGCCTCCTGCACAGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAg        >  2:302368/4‑101 (MQ=255)
                                                                                        ctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGaa      <  2:4389138/100‑1 (MQ=255)
                                                                                        ctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGaa      >  2:3660426/2‑101 (MQ=255)
                                                                                        ctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGaa      <  1:4646997/100‑1 (MQ=255)
                                                                                        ctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGaa      >  2:1478539/2‑101 (MQ=255)
                                                                                        ctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGaa      <  1:3716758/100‑1 (MQ=255)
                                                                                         tGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGaaa     >  1:2211951/1‑101 (MQ=255)
                                                                                         tGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGaaa     <  2:2322983/101‑1 (MQ=255)
                                                                                         tGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGaaa     >  1:1184837/1‑101 (MQ=255)
                                                                                          ggcccAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCTCCGGGTTGTTGGCGCCGATGCCCTGTTCCAGAAAg    <  2:3380476/98‑1 (MQ=255)
                                                                                           gcccAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGAAAgg   <  1:3424749/99‑1 (MQ=255)
                                                                                                   |                                                                                             
TCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAAGGGCTTGA.C.ATTTGGGCCAACAAATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGAAAGGC  >  NC_014034/2123330‑2123520

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG