Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,445 G→A 18.2% A4T (GCA→ACC)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,4450G→A18.2% 85.1 ‑3.6 ‑0.0199A4T (GCA→ACA) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (10/26):  ref base (79/83):  total (90/109)
Fisher's exact test strand distribution p-value = 2.62e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.98e-01

Only 100 of 206 total aligned reads displayed.

GACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGT  >  NC_014034/2155345‑2155545
                                                                                                    |                                                                                                    
gcccgggCCGCGGTCCCCCCCGCCTTCGGAGGCCCGGGGGGGCCTTCGCCGTCGCCGCCCCCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATg                                                                                                      <  2:2997721/96‑1 (MQ=255)
gccccGGCCGCGGTCGCCCTCCCCTTCTGATGCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATg                                                                                                      <  1:2299804/99‑1 (MQ=255)
 ccccGGCCGCGGCCCCCCTCCCCTTCTGATGCCCTGGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGc                                                                                                     <  2:1907878/100‑1 (MQ=255)
  ccgggCCGCGGCCCCCCCCCCCTTCTAATCCCCTCGGGCCCCGTCCCCGCCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCa                                                                                                    <  2:1167602/98‑1 (MQ=255)
  cccGCCCGCGGTCCCCCTCACCTTCTGAGCCCCCCGGGCGCCGCCACCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCa                                                                                                    <  1:4946369/101‑1 (MQ=255)
       ccGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCACgggg                                                                                               >  1:2968358/1‑101 (MQ=255)
       ccGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgggg                                                                                               >  2:247246/1‑101 (MQ=255)
           ggccccccTCCCCTTCTGATGCCCGCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                           <  1:3115940/96‑1 (MQ=255)
           ggTCCCCCTCCCCTTCTGATGCCCTCGGGCGCCGCCACCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                           <  1:4504832/101‑1 (MQ=255)
            gTCGCCCTCCCCTTCTGATGCCCTCGGGCGCCGCCACCGCCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                          <  1:2762913/101‑1 (MQ=255)
            gTCGCCCTCCCCTTCTGATGCCCTCGGGCCCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                          <  2:3181617/101‑1 (MQ=255)
            gTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                          >  2:3952432/1‑101 (MQ=255)
             gcccccTCCCCTTCTGATCCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGt                                                                                         <  1:3064920/98‑1 (MQ=255)
                 ccTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                                     >  2:4411295/1‑101 (MQ=255)
                   tCACCTGTTGATGCCCCGGGCCGCCGTCCCCGTCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCt                                                                                   <  2:3093001/101‑1 (MQ=255)
                      ctttCTGATGCCCGGGGGCGCCGCCACCGTCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgt                                                                                <  2:806474/99‑1 (MQ=255)
                       cTTCGGATCCCCCGGGGCGCCGTCCCCGTCCCCCCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgtc                                                                               <  2:3328182/101‑1 (MQ=255)
                        ttcggacccccGCGGGCGCCGCCACCGTCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCTTAGAGCtcgtcg                                                                              <  1:2733105/93‑1 (MQ=255)
                         tCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                             >  2:1179761/1‑101 (MQ=255)
                         tCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                             >  2:4308350/1‑101 (MQ=255)
                         tCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                             >  2:3446505/1‑101 (MQ=255)
                         tCTGATCCCCCGGGGCGCCGCCCCCGCCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                             <  1:10003/101‑1 (MQ=255)
                          ctgacccccGCGGGCCCGGCCCCCGCCCCCGCCCGCGGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGAt                                                                            <  2:3645222/95‑1 (MQ=255)
                          cTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCACGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCGCGTGGAGCtcgcggat                                                                            >  2:2297565/1‑96 (MQ=255)
                           tGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATg                                                                           >  2:4556896/1‑101 (MQ=255)
                            gccccccgggggCGCCGCCCCCGCCCCCCCCCGCGGGGCGAAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGc                                                                          <  1:4595760/92‑1 (MQ=255)
                            gATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGc                                                                          >  1:1367330/1‑101 (MQ=255)
                            gATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGc                                                                          >  1:3499027/1‑101 (MQ=255)
                             gtGCCCTCGGGCGCCGTCCCCGCCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGcc                                                                         <  1:3997391/100‑1 (MQ=255)
                               gCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCAt                                                                       >  2:3924846/1‑101 (MQ=255)
                                   ccGGGCCCCGCCCCCGCCCCCGCCCGCGGGGCGCAAATCCGCGAAAGGGCAGGATCAAGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgg                                                                   <  1:4314107/100‑1 (MQ=255)
                                     gggCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgggg                                                                 >  2:652074/1‑101 (MQ=255)
                                     gggCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgggg                                                                 >  1:3191507/1‑101 (MQ=255)
                                      ggCGCCGCCCCCGCCCCCGGCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggc                                                                <  1:5036336/101‑1 (MQ=255)
                                       gcgcCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcg                                                               >  2:4350691/1‑101 (MQ=255)
                                           cgtcaccgtcacCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcggggc                                                           >  1:1794183/1‑101 (MQ=255)
                                              ccccgcccccgccggcGGGGCGGAAAGCCGCGAAAGGGCGGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGt                                                        <  2:1846845/93‑1 (MQ=255)
                                               cccgcccccccccgcgGGGGGAAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTc                                                       <  2:4685801/91‑1 (MQ=255)
                                               accgtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTc                                                       >  1:3587221/1‑101 (MQ=255)
                                               accgtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTc                                                       >  2:2189852/1‑101 (MQ=255)
                                                ccgcccccgcccgcGGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTca                                                      <  1:4234601/95‑1 (MQ=255)
                                                 cgtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcac                                                     >  1:373763/1‑101 (MQ=255)
                                                  gtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                                    >  2:2454668/1‑101 (MQ=255)
                                                  gcccccccccgccGCGTGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                                    <  1:1525600/94‑1 (MQ=255)
                                                    caccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcaccac                                                  >  2:4292937/1‑101 (MQ=255)
                                                     cccccccgcgGGGGGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACg                                                 <  2:3818982/97‑1 (MQ=255)
                                                        ccccgccGGGCGACAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACTATg                                              <  2:5008132/100‑1 (MQ=255)
                                                            gccgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcgg                                          <  2:3498757/101‑1 (MQ=255)
                                                             ccgGGCGCCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggc                                         <  2:3248492/101‑1 (MQ=255)
                                                              cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                        >  1:2528911/1‑101 (MQ=255)
                                                               gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                       >  2:2899127/1‑101 (MQ=255)
                                                               gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                       >  1:2236545/1‑101 (MQ=255)
                                                                    gcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                  >  1:3475983/1‑101 (MQ=255)
                                                                    aaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                  <  1:1932877/99‑1 (MQ=255)
                                                                     aaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCg                                 <  2:4128489/100‑1 (MQ=255)
                                                                      aaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGc                                >  1:3399302/1‑101 (MQ=255)
                                                                       aGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGcc                               <  1:558785/101‑1 (MQ=255)
                                                                            cgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcg                          >  2:3030400/1‑101 (MQ=255)
                                                                               aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCTTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCt                       <  1:4351008/101‑1 (MQ=255)
                                                                                  ggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAt                    >  1:2380904/1‑101 (MQ=255)
                                                                                   gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg                   <  1:3425337/101‑1 (MQ=255)
                                                                                   gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg                   >  1:1848175/1‑101 (MQ=255)
                                                                                   agaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtg                   >  1:1260311/9‑101 (MQ=255)
                                                                                    cAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgg                  >  2:715198/1‑100 (MQ=255)
                                                                                      ggATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTgg                <  2:4759520/101‑1 (MQ=255)
                                                                                      ggATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTgg                >  2:841768/1‑101 (MQ=255)
                                                                                      ggATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTgg                >  2:2336511/1‑101 (MQ=255)
                                                                                      aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTgg                >  2:2773070/6‑101 (MQ=255)
                                                                                       gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGCGGc               >  1:1404337/1‑101 (MQ=255)
                                                                                       aaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGc               >  1:1072106/5‑101 (MQ=255)
                                                                                       aaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGGGGc               >  1:4728314/5‑101 (MQ=255)
                                                                                        aTCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCa              >  2:4846322/1‑101 (MQ=255)
                                                                                         tCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAt             <  1:4528216/101‑1 (MQ=255)
                                                                                          cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt            <  1:2413376/101‑1 (MQ=255)
                                                                                          cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt            <  2:4457714/101‑1 (MQ=255)
                                                                                          cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt            <  2:1932518/101‑1 (MQ=255)
                                                                                          cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt            >  1:3875755/1‑101 (MQ=255)
                                                                                          cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt            <  2:3630038/101‑1 (MQ=255)
                                                                                          cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt            >  2:1390765/1‑101 (MQ=255)
                                                                                          aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt            <  2:3150435/100‑1 (MQ=255)
                                                                                          aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt            <  1:4616739/100‑1 (MQ=255)
                                                                                          aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGGGGCAtt            >  2:1635381/2‑101 (MQ=255)
                                                                                           aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGGTTTGGCATTc           >  2:2835596/1‑101 (MQ=255)
                                                                                           aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc           <  1:1683617/101‑1 (MQ=255)
                                                                                           aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc           <  1:2029270/101‑1 (MQ=255)
                                                                                           aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc           <  2:1001096/101‑1 (MQ=255)
                                                                                           aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc           >  1:2464534/1‑101 (MQ=255)
                                                                                           aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc           <  1:81443/101‑1 (MQ=255)
                                                                                           aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc           <  1:670948/101‑1 (MQ=255)
                                                                                            tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg          <  1:4846649/101‑1 (MQ=255)
                                                                                             gaccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGa         >  1:3643140/4‑101 (MQ=255)
                                                                                             gaccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGa         <  2:2712479/98‑1 (MQ=255)
                                                                                               gCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAcc       <  2:4249073/101‑1 (MQ=255)
                                                                                               gCGATGCAAGGGGCCTCGGAGAGGTCGTCGAGGCCATCGGGGGCGGGGCCGCCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAcc       <  1:1345252/101‑1 (MQ=255)
                                                                                               ccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAcc       <  2:2201768/100‑1 (MQ=255)
                                                                                               ccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAcc       <  2:616270/100‑1 (MQ=255)
                                                                                                cGATGCAAGGGGCCTCGTAGAGCGCGGCGATGCCATCTGGGGCGGGGCCGTCACCACGCTGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCg      <  2:2577201/101‑1 (MQ=255)
                                                                                                  aTGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAg    >  2:3538856/1‑101 (MQ=255)
                                                                                                  aTGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAg    >  2:2041180/1‑101 (MQ=255)
                                                                                                    gCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGt  <  2:1764130/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGT  >  NC_014034/2155345‑2155545

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG