Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,463 C→G 31.5% L10V (CTC→GTC)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,4630C→G31.5% 243.8 ‑0.4 ‑0.2260L10V (CTC→GTC) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (41/41):  ref base (94/84):  total (135/125)
Fisher's exact test strand distribution p-value = 6.91e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.71e-01

Only 100 of 270 total aligned reads displayed.

TCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCG  >  NC_014034/2155364‑2155563
                                                                                                   |                                                                                                    
      tCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                                               >  2:3446505/1‑101 (MQ=255)
      tCTGATCCCCCGGGGCGCCGCCCCCGCCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                                               <  1:10003/101‑1 (MQ=255)
       cTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCACGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCGCGTGGAGCtcgcggat                                                                                              >  2:2297565/1‑96 (MQ=255)
         gccccccgggggCGCCGCCCCCGCCCCCCCCCGCGGGGCGAAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGc                                                                                            <  1:4595760/92‑1 (MQ=255)
         gATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGc                                                                                            >  1:3499027/1‑101 (MQ=255)
         gATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGc                                                                                            >  2:3071921/1‑101 (MQ=255)
            ccccGCGGCCCCCGCCCCCGTCCCCGCCCGCCGGGCGCCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCAt                                                                                         <  2:4328034/100‑1 (MQ=255)
                ccGGGCCCCGCCCCCGCCCCCGCCCGCGGGGCGCAAATCCGCGAAAGGGCAGGATCAAGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgg                                                                                     <  1:4314107/100‑1 (MQ=255)
                  gggCGCCGTCTCCGTCACCGCCCGCCGGGCGGCAAGCCCCGCAAGGCCAGGACCATGATCGAGGCACGGGGCCTCGTAGACCTCGTCGCTGCCATCTgggc                                                                                   >  1:1633462/1‑100 (MQ=255)
                  gggCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgggg                                                                                   >  2:3526183/1‑101 (MQ=255)
                   ggCGCCGCCCCCGCCCCCGGCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggc                                                                                  <  1:5036336/101‑1 (MQ=255)
                      gcCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcggg                                                                               >  1:1764260/1‑101 (MQ=255)
                         gtcaccgtcaccGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcgggggc                                                                            >  2:3490405/1‑99 (MQ=255)
                         gtcaccgtcaccGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGcc                                                                            >  1:3099941/1‑101 (MQ=255)
                         gtcaccgtcaccGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCACCTggggcggggcg                                                                            >  2:2471868/1‑100 (MQ=255)
                           caccgtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGt                                                                          >  2:2880378/1‑101 (MQ=255)
                            cccgcccccgcccgcCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCgcg                                                                         <  2:1508831/94‑3 (MQ=255)
                            accgtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTc                                                                         >  2:2189852/1‑101 (MQ=255)
                              cccccccccccgccGGGGGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcac                                                                       <  1:2016096/93‑1 (MQ=255)
                               gtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                                                      >  1:302868/1‑101 (MQ=255)
                                 ccccgcccgcCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcaccac                                                                    <  2:1684928/99‑1 (MQ=255)
                                  cccccccgcgGGGGGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACg                                                                   <  2:3818982/97‑1 (MQ=255)
                                     ccccgccGGGCGACAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACTATg                                                                <  2:5008132/100‑1 (MQ=255)
                                      cccgccGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATgg                                                               >  1:4632391/1‑101 (MQ=255)
                                       ccgcggGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggc                                                              <  1:2334144/101‑1 (MQ=255)
                                          ccgGGCGCCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggc                                                           <  2:3248492/101‑1 (MQ=255)
                                                 gcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                                    <  2:4516518/101‑1 (MQ=255)
                                                  aaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCg                                                   <  2:4128489/100‑1 (MQ=255)
                                                    aGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGcc                                                 <  1:558785/101‑1 (MQ=255)
                                                     gCCGCGAAAGGGCAGGATCATGAGCGATGCGAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCg                                                <  1:3239468/101‑1 (MQ=255)
                                                          gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                                           >  1:2411906/1‑101 (MQ=255)
                                                          gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATCCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                                           >  2:3012038/1‑101 (MQ=255)
                                                           aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                                          >  2:4185257/1‑101 (MQ=255)
                                                            aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCTTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCt                                         <  1:4351008/101‑1 (MQ=255)
                                                            aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCt                                         <  2:74944/101‑1 (MQ=255)
                                                            aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCt                                         >  1:74954/1‑101 (MQ=255)
                                                              ggagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGa                                       <  1:4822812/91‑1 (MQ=255)
                                                              ggagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGa                                       >  1:862716/11‑101 (MQ=255)
                                                                gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGGTGAtg                                     >  1:4279215/1‑101 (MQ=255)
                                                                gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg                                     >  1:1352962/1‑101 (MQ=255)
                                                                gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg                                     <  1:3425337/101‑1 (MQ=255)
                                                                gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg                                     >  1:1848175/1‑101 (MQ=255)
                                                                  aaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgtg                                   <  2:2806749/95‑1 (MQ=255)
                                                                  aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgtg                                   >  1:2760662/1‑101 (MQ=255)
                                                                   ggATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTgg                                  >  2:841768/1‑101 (MQ=255)
                                                                   ggATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTgg                                  >  1:2082393/1‑101 (MQ=255)
                                                                     aTCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCa                                >  2:4846322/1‑101 (MQ=255)
                                                                      tCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGGGGCGGCGCTCGCCGGGCGGTTGATGTGGCAt                               >  2:4723196/1‑101 (MQ=255)
                                                                       cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt                              <  2:3630038/101‑1 (MQ=255)
                                                                       aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt                              <  2:4301066/100‑1 (MQ=255)
                                                                       aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt                              <  2:3150435/100‑1 (MQ=255)
                                                                        aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc                             <  1:3458932/101‑1 (MQ=255)
                                                                        aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc                             >  1:3650219/1‑101 (MQ=255)
                                                                        aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc                             <  2:1890265/101‑1 (MQ=255)
                                                                        aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc                             >  1:2464534/1‑101 (MQ=255)
                                                                        aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc                             <  2:1172435/101‑1 (MQ=255)
                                                                         tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg                            >  1:1324401/1‑101 (MQ=255)
                                                                           accGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGAGCCTTTCgtc                          <  2:3929605/99‑3 (MQ=255)
                                                                            gCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAcc                         <  2:4249073/101‑1 (MQ=255)
                                                                            ccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAcc                         <  2:616270/100‑1 (MQ=255)
                                                                             cgagaccagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg                        <  2:3733000/91‑1 (MQ=255)
                                                                             cGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCg                        <  2:4564681/101‑1 (MQ=255)
                                                                             cGATGCAAGGGGCCTCGTAGAGCGCGGCGATGCCATCTGGGGCGGGGCCGTCACCACGCTGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCg                        <  2:2577201/101‑1 (MQ=255)
                                                                              gataccagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGa                       >  2:4805956/10‑101 (MQ=255)
                                                                              gataccagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGa                       <  1:4401897/92‑1 (MQ=255)
                                                                               ataccagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAg                      >  1:2688014/9‑101 (MQ=255)
                                                                               aTGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAg                      >  2:2041180/1‑101 (MQ=255)
                                                                                taccagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAgg                     >  2:4358537/8‑101 (MQ=255)
                                                                                taccagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAgg                     >  2:2690260/8‑101 (MQ=255)
                                                                                taccagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAgg                     >  1:3941633/8‑101 (MQ=255)
                                                                                 accagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGCCCGGGCGCCTGATGTGGCATTCGACCGAggg                    >  1:1391076/7‑100 (MQ=255)
                                                                                  ccagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTg                   <  1:4026223/96‑1 (MQ=255)
                                                                                  ccagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTg                   <  2:1091050/96‑1 (MQ=255)
                                                                                  cAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTg                   <  2:1435797/101‑1 (MQ=255)
                                                                                   cagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGc                  >  2:1813733/5‑101 (MQ=255)
                                                                                   cagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGc                  >  1:2278191/5‑101 (MQ=255)
                                                                                   aaGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGc                  >  2:4660243/1‑101 (MQ=255)
                                                                                   aaGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGc                  <  1:3246776/101‑1 (MQ=255)
                                                                                     ggggCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCgg                >  2:253168/1‑101 (MQ=255)
                                                                                     ggggCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCgg                >  1:3829645/1‑101 (MQ=255)
                                                                                     ggggCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCgg                >  2:557582/1‑101 (MQ=255)
                                                                                     ggggCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGGGCgg                >  2:3386435/1‑101 (MQ=255)
                                                                                     gaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCgg                <  1:4789499/99‑1 (MQ=255)
                                                                                     gaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCgg                <  1:3530322/99‑1 (MQ=255)
                                                                                       ggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgc              <  1:251614/101‑1 (MQ=255)
                                                                                       ggCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGgc              >  1:1099830/1‑101 (MQ=255)
                                                                                        gCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGgca             >  2:2769121/1‑100 (MQ=255)
                                                                                          cTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgcg           >  2:1321424/1‑101 (MQ=255)
                                                                                           tCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGgcgcgc          <  1:2819493/101‑1 (MQ=255)
                                                                                            cGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGCGCGGgcgcggc         >  2:2950223/1‑99 (MQ=255)
                                                                                            cGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCa         <  1:1933050/101‑1 (MQ=255)
                                                                                            cGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCa         <  1:4185574/101‑1 (MQ=255)
                                                                                             gTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCaa        >  1:149913/1‑101 (MQ=255)
                                                                                             gTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCaa        >  2:2349094/1‑101 (MQ=255)
                                                                                             gTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCaa        <  1:1165837/101‑1 (MQ=255)
                                                                                             gTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCaa        >  1:4891364/1‑101 (MQ=255)
                                                                                              tAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCCGGCGGCTGGTGTGGCATTCGACCGAGGGGCGGGCGCGCAAg       >  1:3729212/1‑101 (MQ=255)
                                                                                               agagCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAgc      >  1:4536325/1‑101 (MQ=255)
                                                                                                  gCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGcgcc   <  2:3582294/101‑1 (MQ=255)
                                                                                                   cTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGCCGGGCAAGccccg  >  1:3343893/1‑101 (MQ=255)
                                                                                                   |                                                                                                    
TCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCG  >  NC_014034/2155364‑2155563

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG