Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,712 C→G 14.8% Q93E (CAG→GAG)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,7120C→G14.8% 82.8 ‑1.3 ‑0.1236Q93E (CAG→GAG) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/16):  ref base (89/112):  total (108/128)
Fisher's exact test strand distribution p-value = 2.78e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.78e-01

Only 100 of 255 total aligned reads displayed.

GGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCTCCGGGCGCTCGGCTTTGAG  >  NC_014034/2155612‑2155811
                                                                                                    |                                                                                                   
ggCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCCGCCCGCGCGGCGccc                                                                                                     >  2:426900/1‑101 (MQ=255)
 gCGATCATCGGCGGGGCGGTGGCGGCTTATTTCGAGCTCGGTCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCa                                                                                                    <  1:929885/101‑1 (MQ=255)
  cGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAg                                                                                                   <  1:717314/101‑1 (MQ=255)
  cGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGACCAg                                                                                                   <  1:1025173/101‑1 (MQ=255)
    atcatcGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAggg                                                                                                 >  1:2137585/1‑100 (MQ=255)
    atcatcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGt                                                                                                 <  1:2472042/101‑1 (MQ=255)
    atcatcGGCGAGGCGGGGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGt                                                                                                 <  2:4348289/101‑1 (MQ=255)
       atcGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAggggct                                                                                              >  1:3616032/1‑97 (MQ=255)
       atcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGgc                                                                                              >  1:4018329/1‑101 (MQ=255)
       atcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGgc                                                                                              >  2:3553647/1‑101 (MQ=255)
        tcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGgcg                                                                                             <  1:1774073/101‑1 (MQ=255)
         cGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgctgc                                                                                            <  1:2276021/101‑5 (MQ=255)
         cGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCCCCCAGGTGgcgc                                                                                            <  1:2731364/101‑1 (MQ=255)
          ggCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgctgat                                                                                           <  1:2462217/101‑6 (MQ=255)
           gCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTg                                                                                          >  2:1281681/1‑101 (MQ=255)
           gCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTg                                                                                          <  2:4832633/101‑1 (MQ=255)
           gCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTg                                                                                          <  1:3069288/101‑1 (MQ=255)
           gCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTg                                                                                          <  2:347445/101‑1 (MQ=255)
             gAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAc                                                                                        <  2:4192679/101‑1 (MQ=255)
              aGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatg                                                                                       <  2:2517934/101‑3 (MQ=255)
              aGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAcc                                                                                       <  1:1173479/101‑1 (MQ=255)
                gCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgag                                                                                     <  1:4881131/101‑5 (MQ=255)
                gCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCg                                                                                     <  1:4213065/101‑1 (MQ=255)
                 gggTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgagg                                                                                    <  2:1071993/100‑6 (MQ=255)
                 cGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCgg                                                                                    >  1:3975877/1‑101 (MQ=255)
                  ggTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgagga                                                                                   >  2:194606/1‑95 (MQ=255)
                  ggTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgagga                                                                                   >  2:218375/1‑95 (MQ=255)
                  ggTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgagga                                                                                   >  1:1913140/1‑95 (MQ=255)
                  ggTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCggg                                                                                   >  1:19096/1‑101 (MQ=255)
                   gTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgaggat                                                                                  <  2:4687658/101‑8 (MQ=255)
                   gTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGt                                                                                  <  2:4518374/101‑1 (MQ=255)
                   gTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGt                                                                                  >  2:2957692/1‑101 (MQ=255)
                    tGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGCGGTGCTGCTGatgaggatc                                                                                 >  1:3755658/1‑93 (MQ=255)
                    tGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtc                                                                                 >  2:2623488/1‑101 (MQ=255)
                    tGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtc                                                                                 <  2:4018026/101‑1 (MQ=255)
                     ggcggcTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGCGCTGCTGatgaggatca                                                                                >  1:3948961/1‑92 (MQ=255)
                      gcggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtctt                                                                               <  1:2835809/101‑1 (MQ=255)
                       cggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtcttc                                                                              <  2:2807032/101‑1 (MQ=255)
                       cggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtcttc                                                                              >  2:4147954/1‑101 (MQ=255)
                        ggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCACGCGGCGCCCAGGTGGCGCTGACCCGGGtcttct                                                                             >  1:4339577/1‑101 (MQ=255)
                         gcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTcttctt                                                                            <  2:465664/101‑1 (MQ=255)
                          cTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCttt                                                                           >  1:1313679/1‑101 (MQ=255)
                          cTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCttt                                                                           <  2:3047418/101‑1 (MQ=255)
                          cTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCttt                                                                           <  1:2753838/101‑1 (MQ=255)
                           ttATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTc                                                                          <  1:4082054/101‑1 (MQ=255)
                           ttATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTc                                                                          <  1:2597946/101‑1 (MQ=255)
                            tagttCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTca                                                                         >  1:2883737/4‑101 (MQ=255)
                            tATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTca                                                                         >  1:1861864/1‑101 (MQ=255)
                             aTTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTcac                                                                        <  1:3887229/101‑1 (MQ=255)
                             aTTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTcac                                                                        >  1:4844634/1‑101 (MQ=255)
                              tttCGAGCTCGGCCGGACCGTGACCCCCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTcaca                                                                       <  2:3188020/101‑1 (MQ=255)
                                tCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCacaac                                                                     <  2:1794050/101‑1 (MQ=255)
                                tCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCacaac                                                                     >  1:3492287/1‑101 (MQ=255)
                                 cGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCacaaca                                                                    <  2:3300102/101‑1 (MQ=255)
                                  gAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCAcaacaa                                                                   >  2:1454750/1‑101 (MQ=255)
                                   aGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAg                                                                  >  2:3861029/1‑101 (MQ=255)
                                    gCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAgg                                                                 <  2:1104256/101‑1 (MQ=255)
                                      tCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAg                                                               <  1:4660566/101‑1 (MQ=255)
                                       cGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGt                                                              >  1:1100630/1‑101 (MQ=255)
                                          ccGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGt                                                           >  2:511140/1‑101 (MQ=255)
                                            ggACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTga                                                         <  2:4891037/101‑1 (MQ=255)
                                             gACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTgag                                                        <  2:2082245/101‑1 (MQ=255)
                                               ccGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGAt                                                      >  2:4998892/1‑101 (MQ=255)
                                                cctGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATg                                                     <  1:1610156/99‑1 (MQ=255)
                                                cGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATg                                                     <  1:1516325/101‑1 (MQ=255)
                                                  tGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgac                                                   <  1:4740964/101‑1 (MQ=255)
                                                  tGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgac                                                   <  1:2938204/101‑1 (MQ=255)
                                                   gACCGCCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgacg                                                  <  1:1186952/101‑1 (MQ=255)
                                                   gACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgacg                                                  <  1:945247/101‑1 (MQ=255)
                                                          ggCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACt                                           >  2:2333088/1‑101 (MQ=255)
                                                             gtcgtcGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGc                                        >  1:820929/1‑101 (MQ=255)
                                                             gtcgtcGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGc                                        >  2:4782623/1‑101 (MQ=255)
                                                               cgtcgCAACGCTCGCCTATCTCGGCCCGCGCGGCTCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCgg                                      >  1:980011/1‑101 (MQ=255)
                                                               cgtcgCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCgg                                      >  1:2104126/1‑101 (MQ=255)
                                                               cgtcgCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCgg                                      >  2:1417625/1‑101 (MQ=255)
                                                                  cgCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCggggc                                   >  1:2354782/1‑99 (MQ=255)
                                                                  cgCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGcc                                   <  1:1417752/101‑1 (MQ=255)
                                                                    cAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGcgg                                 >  1:3148888/1‑100 (MQ=255)
                                                                    cAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCcgc                                 >  2:1631002/1‑101 (MQ=255)
                                                                     aaCGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGcgcg                                >  2:927600/1‑101 (MQ=255)
                                                                     aaCGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCcgcc                                >  1:5045033/1‑100 (MQ=255)
                                                                      aCGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCgcgc                               <  2:3687731/101‑1 (MQ=255)
                                                                        gCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCCTGAGATGACGACAGACTGGCGGGCCGCGCAg                             <  1:4477196/101‑1 (MQ=255)
                                                                         cTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGc                            <  1:536175/101‑1 (MQ=255)
                                                                         cTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGc                            <  1:4948235/101‑1 (MQ=255)
                                                                           cGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGCgcgcgcccg                          >  1:3278543/1‑96 (MQ=255)
                                                                           cGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCCGCTg                          >  2:278559/1‑101 (MQ=255)
                                                                           cGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTg                          <  2:4933256/101‑1 (MQ=255)
                                                                           cGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTg                          <  1:3736726/101‑1 (MQ=255)
                                                                             ccTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGCGCGCAggcgcg                        >  1:752362/1‑96 (MQ=255)
                                                                                aTCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGCGCGCGGCCGCgggc                     >  1:2704559/1‑99 (MQ=255)
                                                                                aTCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGCGCGCCGCGGCgggc                     >  2:3911463/1‑99 (MQ=255)
                                                                                   tcGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCTCCg                  <  2:752272/101‑1 (MQ=255)
                                                                                        ccGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCTCCGGgcgc             <  2:2467749/101‑1 (MQ=255)
                                                                                        ccGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCCCCCAGCCACGGCACCCGgccc             >  1:1893200/1‑99 (MQ=255)
                                                                                               gcgcCCAGGTGGCGCTGGCCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCTCCGGGCGCTCGGCtt      <  1:1239616/101‑1 (MQ=255)
                                                                                                cgcCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCTCCGGGCGCTCGGCttt     <  2:667322/101‑1 (MQ=255)
                                                                                                   ccAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGCGCGCCGCTGGGGCCCCCGGGGCGCGGCGTTGAg  >  1:1056420/1‑101 (MQ=255)
                                                                                                   ccAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCTCCGGGCGCTCGGCTTTGAg  <  2:4590992/101‑1 (MQ=255)
                                                                                                   ccAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCCCGCCGCCGCGGCCCCGGGCGCCCGGCTTTggg  >  1:4510119/1‑99 (MQ=255)
                                                                                                    |                                                                                                   
GGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGGCTCCGGGCGCTCGGCTTTGAG  >  NC_014034/2155612‑2155811

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG