Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,186,002	G→C	100%	intergenic (+275/‑230)	mtlK → / → RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,186,002	0	G→C	100.0%	103.4	33	intergenic (+275/‑230)	mtlK/RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (14/19):  ref base (0/0):  total (14/19)

ACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..CCC...GC.CGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACC  >  NC_014034/2185920‑2186099                                                                                            |                                                                                                  aCGCCGACCCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAcccc                                                                                  <  2:4712730/101‑1 (MQ=2)      gAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC...GG.CGGGGCGCCGCCGGG.G.AAGGCCGAGGCGGAAAAcccccccgc                                                                             >  1:638168/1‑96 (MQ=2)         ccccccccTTCCACCTTGGCCAAAAGACCCCGGGGGCCGGGGGCCGTA..GC.CCC...TCGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCac                                                                          <  2:4195431/97‑1 (MQ=2)                 tttCACCTGGGCTCAAATACCCCGGGGGGCCGGGGGCGGA..GCCCCC...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACcgaccgac                                                                  <  1:1354669/101‑1 (MQ=9)                     cccTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGc                                                              <  2:2483931/100‑1 (MQ=9)                     cccTTGGCTCAAATACCCGGGGGGCCGGGGGGCGGACCC..CCC...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGc                                                              <  2:4581361/100‑1 (MQ=9)                      ccTTGGCTCAAATACCCCGGGGGTCCGGGGGGGGACCC..CCC...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCg                                                             <  2:2305330/101‑1 (MQ=9)                        ttGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGAc                                                           <  1:2471680/101‑1 (MQ=14)                                   atCCCGGGGGTCCGGGGGCGGA..GCCCCC...GG.CGGGGCGCCCCCGG..CCAAGCCCCAGGCGAAAAACCCCAGCGCCCCCCCCCCACCTGCGACGGCCCGGcgcg                                                >  2:4058630/1‑101 (MQ=2)                                     cccGGGGGGCCGGGGGCGGA..G..CCCCCGGG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCgcgc                                               <  1:737388/101‑1 (MQ=12)                                        gggggTCCGGGGGCGGA..GCCCCC...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCaaaa                                           <  1:1619847/101‑1 (MQ=18)                                                     ggcggacccccc...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcagg                                <  2:631719/92‑1 (MQ=21)                                                       cggagccccc...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggca                              <  1:2714778/94‑1 (MQ=21)                                                                                cctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAcc           >  1:77378/10‑101 (MQ=255)                                                                                cctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAcc           <  2:1261239/92‑1 (MQ=255)                                                                                cctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAcc           >  1:2619064/10‑101 (MQ=255)                                                                                 ctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAccc          >  1:4896283/9‑101 (MQ=255)                                                                                 ctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAccc          >  1:1442725/9‑101 (MQ=255)                                                                                   cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCtt        >  1:2288401/7‑101 (MQ=255)                                                                                    gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCTTTGACCCTTc       >  2:229589/6‑101 (MQ=255)                                                                                    gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc       <  2:2825219/96‑1 (MQ=255)                                                                                    gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc       <  1:3881146/96‑1 (MQ=255)                                                                                    gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc       >  2:4496888/6‑101 (MQ=255)                                                                                     ggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCg      <  1:1728182/97‑1 (MQ=255)                                                                                     ggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACGTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCCTCg      >  2:867851/5‑101 (MQ=255)                                                                                      gcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCgg     <  2:1935390/98‑1 (MQ=255)                                                                                      gcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCgg     >  2:3629111/4‑101 (MQ=255)                                                                                      gcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCgg     <  1:4960547/98‑1 (MQ=255)                                                                                       caaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGa    >  1:1134741/3‑101 (MQ=255)                                                                                        aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc   >  2:1056730/2‑101 (MQ=255)                                                                                        aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc   >  1:3985588/2‑101 (MQ=255)                                                                                        aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc   <  1:2421863/100‑1 (MQ=255)                                                                                         aaGCCCGAGGCGAAACCCCCCAGCGCCACCGCCCGGCTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGGcc  <  2:2176489/101‑1 (MQ=255)                                                                                         aaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAcc  >  1:3329145/1‑101 (MQ=255)                                                                                         aaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAcc  <  1:3292523/101‑1 (MQ=255)                                                                                         aaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAcc  >  1:3277916/1‑101 (MQ=255)                                                                                            |                                                                                                  ACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..CCC...GC.CGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACC  >  NC_014034/2185920‑2186099 Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG