Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 222,069 G→C 25.8% T196T (ACC→ACG RCAP_rcc00185 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034222,0690G→C25.8% 0.0 ‑0.7 ‑0.0556T196T (ACC→ACGRCAP_rcc00185hypothetical protein
Reads supporting (aligned to +/- strand):  new base (74/70):  ref base (226/186):  total (300/256)
Fisher's exact test strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.54e-01

Only 100 of 782 total aligned reads displayed.

GTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACA  >  NC_014034/221962‑222169
                                                                                                           |                                                                                                    
       ccgtgggatcggtcgggtccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGGCGGGTCCGTGGGAtcg                                                                                                      >  2:3970353/1‑101 (MQ=11)
       ccgtgggatcggtcgggtccgtcggatcggtcgtGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcg                                                                                                      <  2:441502/101‑1 (MQ=14)
        cgtgggatcggtcgggtccgtgggatcggtcggGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGGCCGTGGGAtcgg                                                                                                     >  1:3805788/1‑101 (MQ=11)
        cgtgggatcggtcgggtccgtgggatcggtcggGTCCGGGGGATCGGTCGGGTCCGTGGGGTCCGTGGGATCCGGGGGATCGGTCGGCTCCGTGGGAtcgg                                                                                                     >  1:1234687/1‑101 (MQ=11)
        cgtgggatcggtcgggtccgtcggatcggtcggGTCCGTGGGGTCGGTCGGGTCCGTCGGATCGGTGGGGTCCGTGGGATCGGTCGGGTGCGTGGGGtcgg                                                                                                     >  1:2583691/1‑101 (MQ=11)
        cgggggatcggtcgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcgg                                                                                                     <  1:4862407/98‑1 (MQ=11)
         gtgggttcggtcgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggt                                                                                                    <  2:3368208/101‑1 (MQ=11)
         gtggggtgggtcggacacgtgggatcggtcggCCCCGTGGGAACGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGAtcggt                                                                                                    <  2:2014376/101‑1 (MQ=11)
          tgggatcggtcgggtccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGGtcggtg                                                                                                   >  2:2933304/1‑100 (MQ=14)
          tgggatcggtcagatccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGCTCCGTGGGAgcggtc                                                                                                   >  2:1246283/1‑101 (MQ=11)
           gggatcggtcgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTGGGGTCGGTGGGGccggtgg                                                                                                  >  1:2276516/1‑99 (MQ=11)
           gggatcggtcgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGGCGGGTCCGTGGGAtcggtcg                                                                                                  >  2:2533318/1‑101 (MQ=11)
            ggggcggtcgggtccgggggatcggtcggAGCCGTGGTATCGGTCGGATCCGTAGGAACGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGAtcggtcgg                                                                                                 <  1:4314983/97‑1 (MQ=11)
            ggatcggtcgggtccgtgggatcggtcggGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGCtcggtcgg                                                                                                 >  1:748487/1‑101 (MQ=14)
            ggatcggtcgggtccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTGGGATCCGTGGGATCGGTGGGGTCGGTGGGGtcgggcgg                                                                                                 >  2:2173396/1‑101 (MQ=14)
            ggatcggtcgggtccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGGGGGAtcggtcgg                                                                                                 >  1:4586031/1‑101 (MQ=11)
            ggatcggtcgggtccgtcggatcggtgggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGGCGGGTCCGTGGGGtcgggcgg                                                                                                 >  2:3594447/1‑101 (MQ=11)
            ggatcggccgggtcggtgggatcggtgggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGAtcggtcgg                                                                                                 <  2:2108268/101‑1 (MQ=11)
            ggaggggtcgggtccgtgggggccgtcggATCCGTGGGAGCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcgg                                                                                                 <  1:1777129/96‑1 (MQ=11)
              ggcggtcggggccgtgggagcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGAtcggtcggat                                                                                               <  1:1948654/99‑3 (MQ=11)
              atcggtgggatccgtgggatcggccggGTCCGGGGGATCGGTCGGGTCCGTGGGACCGGTCAGGGCCGTGGTATCGGTCGGGGCCGTGGGTtgggtcggcc                                                                                               >  2:850949/1‑99 (MQ=2)
               tctgtcggggccgtcggatcggtgggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGgtcgggtc                                                                                              <  2:900145/98‑1 (MQ=11)
               tcggtcgggtccgtcggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtc                                                                                              <  1:3362093/101‑1 (MQ=11)
                cggtcgggtccgtcggatcggtgggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGGCCGTGGGATCGgtcggggcc                                                                                             >  1:3891967/1‑101 (MQ=11)
                 cggcggatccgtcggatccgtgggATCCGTGGGATCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGgtcgggtccg                                                                                            <  2:4798554/98‑1 (MQ=14)
                  gtcgggtccgtcggatcggtgggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgt                                                                                           >  1:608207/1‑101 (MQ=11)
                  gtcgggtccgtcggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtcggt                                                                                           >  2:3267742/1‑101 (MQ=11)
                   tcgggtccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCTTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGgccggggccgtg                                                                                          >  1:4531524/1‑100 (MQ=11)
                   tcgggtccgtcggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGGTCGgtcgggtccgtc                                                                                          >  1:900250/1‑101 (MQ=11)
                   gcggatccgtaggagcggtctgGTCCGTAGGATCGGTCGGGTCCGTCGGATCTGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtccgtc                                                                                          <  1:2172192/100‑1 (MQ=11)
                    cgggtccgtcggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtcg                                                                                         >  1:1491952/1‑101 (MQ=11)
                    cggatccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtccgtcg                                                                                         <  2:4954763/101‑1 (MQ=11)
                     ggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgaccg                                                                                        >  1:2636302/4‑97 (MQ=11)
                     ggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcgg                                                                                        >  1:1099110/4‑101 (MQ=255)
                      gatccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGAAGGATGGGTCGGGTCCGTAGGATCGgtcgggcccgtcgga                                                                                       >  1:4736861/3‑100 (MQ=17)
                      gatccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtccgtcggg                                                                                       >  1:672499/3‑101 (MQ=11)
                       atccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtccgtcgggt                                                                                      >  2:2363551/2‑101 (MQ=11)
                         cggtgggatcggtcggATCGGTTGGATCCGTCGGGTCCGTGGGATCGGTCGGGTCTGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtggggtcc                                                                                    <  2:4601842/99‑1 (MQ=11)
                           gtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTcgggtccgtcggatcggt                                                                                  >  2:1307959/1‑101 (MQ=11)
                           gtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGGCGGGTCCGTCGGATCCGTggggtccgtcggggccgt                                                                                  >  1:425821/4‑101 (MQ=11)
                            tggggtccggcggACCCGTGTGATCGGTAGGATCCGGGGGATCGGTCGGGTCCGTGGGTTCCGTCGGATCCGTGGGATCGGTcggatccgtgggatcggtc                                                                                 <  2:576010/101‑2 (MQ=11)
                            taggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGGCCGTg                                                                                 >  1:2092185/3‑101 (MQ=11)
                              gggtccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTggg                                                                               >  1:4931036/10‑101 (MQ=11)
                              gggtccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGTTCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTggg                                                                               >  2:2765094/7‑101 (MQ=11)
                              ggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGGCGGGTCCGTGGGATCGGGCGGGTCCGTCGGGTCCGTggg                                                                               >  2:3172465/1‑101 (MQ=17)
                              gctgccgtcgtATCCGTCGGATCGGTCGGCTCCGGCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTcgg                                                                               <  2:2669122/95‑1 (MQ=11)
                                gtccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGac                                                                             >  2:4042010/8‑100 (MQ=11)
                                atcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAt                                                                             <  1:3295346/101‑1 (MQ=11)
                                atcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTggggt                                                                             >  2:4669019/1‑99 (MQ=11)
                                  cggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcg                                                                           >  1:840959/1‑101 (MQ=11)
                                  cggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCAGTCGGCTCCGTGGGGTCCGTCGGGTCCGTgggctgg                                                                           >  1:1589630/1‑97 (MQ=14)
                                  ccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtcg                                                                           <  1:1020335/96‑1 (MQ=14)
                                   ggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGGCCGTCGGGTCCGTGGGAtcgg                                                                          >  2:3705207/1‑101 (MQ=14)
                                     tcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcggtc                                                                        <  1:2898142/101‑1 (MQ=11)
                                      gggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtcggtcg                                                                       >  1:993528/2‑101 (MQ=17)
                                      cggggccGGGGGATCGGTCGGGTTGGTCGGGGGGGTCGGATGCGTGGGTTCGGTCGGGGCGGTCGGATCCGTGGGGTCCGTCGGTTCCGTGGGAtcggtcg                                                                       <  1:4414502/96‑1 (MQ=2)
                                      cggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAtccgtgg                                                                       <  1:2461276/101‑3 (MQ=11)
                                      cggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAtccgtgg                                                                       >  2:1696599/1‑99 (MQ=11)
                                       ggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGCCGGAtccgtggg                                                                      <  1:2739270/101‑1 (MQ=11)
                                        gATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGa                                                                     >  2:1860607/1‑101 (MQ=17)
                                        gATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAtccctggga                                                                     >  1:4136061/1‑94 (MQ=11)
                                          tCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATc                                                                   <  2:2609418/101‑1 (MQ=11)
                                           cggtcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATcc                                                                  <  1:2392249/96‑1 (MQ=17)
                                           ccGTGGGATCGGTCGGGTCGGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTcc                                                                  <  1:3507798/101‑1 (MQ=14)
                                            ggtcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGACCGGTCGGAGCCg                                                                 >  2:1631666/5‑101 (MQ=17)
                                            cgggggATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGACCCGACCGATccc                                                                 <  2:111242/98‑2 (MQ=14)
                                            cGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCg                                                                 <  1:1282700/101‑1 (MQ=14)
                                              tGGGACCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGGTGCGTGGGGCCCGTCGGAGGGGTGGGGTCGGCGGGATcgttg                                                               >  2:4157416/1‑97 (MQ=2)
                                               aggATCGGTCGGGCCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGGCGGGTTCGtcg                                                              >  2:4217022/2‑99 (MQ=14)
                                                ggctcggtcggGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTggg                                                             <  2:490447/98‑1 (MQ=14)
                                                ggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTcgg                                                             >  1:4202411/1‑101 (MQ=14)
                                                ggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTcgg                                                             <  2:2999939/101‑1 (MQ=14)
                                                  aTCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAt                                                           <  2:3505279/101‑1 (MQ=255)
                                                      gtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCAGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcggt                                                       <  1:365227/101‑1 (MQ=14)
                                                         gggTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcggtcgg                                                    <  2:1440954/101‑1 (MQ=14)
                                                          ggTCCGTGGGATCGGTGGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCggg                                                   <  1:5046256/101‑1 (MQ=255)
                                                             ccGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTcc                                                <  1:3987975/101‑1 (MQ=255)
                                                             ccGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTcc                                                <  2:1039859/101‑1 (MQ=255)
                                                             ccGTGGGATCGGTCGGGCCCGTGGGATCGGTCGGATCCGTGGGCTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTcc                                                <  2:1382709/101‑1 (MQ=11)
                                                              cGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCg                                               >  1:3213100/1‑101 (MQ=255)
                                                              cGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCg                                               <  2:3887955/101‑1 (MQ=17)
                                                               gTGGGATCTGGCGGATCCGTGGGGTCGGTGGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGt                                              <  2:2448758/101‑1 (MQ=17)
                                                               gTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGt                                              >  1:1382116/1‑101 (MQ=255)
                                                               gTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGCTCGGTCGGGTCCGt                                              >  2:75728/1‑101 (MQ=17)
                                                               gTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGGCCGTGGGATCGGTCGCGTCCGTGGGACCGGCCGGGTCCgc                                              >  2:2084822/1‑100 (MQ=17)
                                                                 gggATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGCgg                                            >  2:4532456/1‑101 (MQ=255)
                                                                 gggATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGAACCGGGGGAGCGGTCgggggcgggg                                            >  2:4342439/1‑94 (MQ=18)
                                                                 cggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgg                                            >  2:1534303/2‑101 (MQ=17)
                                                                   gATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTagga                                          >  1:47946/1‑100 (MQ=17)
                                                                    aTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggat                                         >  1:3739933/1‑99 (MQ=18)
                                                                    aTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggat                                         <  1:4646883/101‑3 (MQ=14)
                                                                      cggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcg                                       >  1:1439575/1‑97 (MQ=14)
                                                                         tcggGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtggtg                                    >  1:3042187/1‑101 (MQ=255)
                                                                           gggtCCGTGGGATCGGTCGGGTCCGTAGGGTCGGTTTGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGCGACCGGTCGGGCCCACGGGGCTGGGggg                                  >  2:2839076/4‑101 (MQ=18)
                                                                           ggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcggtcgg                                  >  1:1664345/1‑92 (MQ=14)
                                                                                 gTGGGGTCGGTGGGTTCCGTCGGGTCGGTCGGGTCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcggt                            <  2:635268/101‑1 (MQ=17)
                                                                                  tGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGAGCCGTGGGATCGGTCGGGTCCGTGGGGCTGGGGGGAtcggtc                           >  2:677784/1‑101 (MQ=255)
                                                                                   gggATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcggtcg                          >  2:3796161/1‑101 (MQ=255)
                                                                                       tcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGAcc                      <  2:5030551/101‑1 (MQ=255)
                                                                                             gggTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTc                <  1:4058900/101‑1 (MQ=255)
                                                                                                           |                                                                                                    
GTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACA  >  NC_014034/221962‑222169

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG