Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 222,075 G→A 23.1% D194D (GAC→GAT RCAP_rcc00185 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034222,0750G→A23.1% 249.4 ‑0.7 0.0419D194D (GAC→GATRCAP_rcc00185hypothetical protein
Reads supporting (aligned to +/- strand):  new base (59/38):  ref base (181/140):  total (240/179)
Fisher's exact test strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 702 total aligned reads displayed.

TGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCA  >  NC_014034/221972‑222175
                                                                                                       |                                                                                                    
     tcggtcgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcggatc                                                                                                    <  2:4952511/101‑1 (MQ=11)
      cggtcgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcggatcc                                                                                                   >  1:2427144/1‑101 (MQ=11)
      cggtcgggtccgtcggatcggtgggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGGCCGTGGGATCGgtcggggcc                                                                                                   >  1:3891967/1‑101 (MQ=11)
      cggtcggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtcc                                                                                                   <  1:2618574/101‑1 (MQ=255)
       ggtcggctccctgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGCTCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccg                                                                                                  <  2:2679493/101‑1 (MQ=11)
        gtcgggtccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGGCGGGTCGGGGGGATCGATCGGGTCCGTGGGGTCGgccgggtcggt                                                                                                 >  2:2193405/1‑101 (MQ=11)
        gtcgggtccgtcggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtcggt                                                                                                 >  2:3267742/1‑101 (MQ=11)
         tcgggtccgtcggatcggtgggATCCGTGGGATCGGTCGGGTCAGTGGGATCGGTCGGGCCCGTCGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtg                                                                                                <  2:2981797/101‑2 (MQ=11)
           gggtccgtcggatcggtgggATCCGTGGGATGGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtggg                                                                                              <  1:4667223/101‑1 (MQ=11)
           ggatccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGGCGGGTCCGTGGGGGCGGTCGGGTCCGTGGGATCGgtcggctccgtggg                                                                                              >  2:1069516/4‑101 (MQ=11)
           ggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcgg                                                                                              >  1:1099110/4‑101 (MQ=255)
           ggatccgtgggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGgtcgggtccgtcgg                                                                                              >  2:674740/4‑101 (MQ=11)
            gatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcgga                                                                                             <  1:1518134/99‑2 (MQ=255)
            gatccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtccgtcggg                                                                                             >  1:672499/3‑101 (MQ=11)
               ccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtggggtcc                                                                                          <  1:4597365/101‑1 (MQ=11)
                cgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCGGTCGGGTCCCTGGGGTCGgtcggggccgtggggaagt                                                                                         >  2:4759017/1‑97 (MQ=11)
                 gtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTcgggtccgtcggatcggt                                                                                        >  2:4311654/1‑101 (MQ=11)
                  taggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTg                                                                                       <  1:2714492/99‑1 (MQ=14)
                   gggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTcgggtccgtcggatcggtcg                                                                                      >  2:5016845/1‑99 (MQ=11)
                   gggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTgg                                                                                      >  1:755246/1‑101 (MQ=11)
                   cgggtccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGCCCGGTCGGGTCCGCGGGGCCCGTgg                                                                                      >  2:2456872/11‑101 (MQ=2)
                   cgggtccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTgg                                                                                      >  1:4887532/11‑101 (MQ=11)
                    gggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTcgg                                                                                     <  1:2948822/95‑1 (MQ=11)
                    ggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTcgg                                                                                     <  1:121769/101‑1 (MQ=11)
                    ggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTggg                                                                                     <  2:1835309/101‑1 (MQ=11)
                    ggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGGCGGGTCCGTGGGATCGGGCGGGTCCGTCGGGTCCGTggg                                                                                     >  2:3172465/1‑101 (MQ=17)
                    ggatcgggcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTcgg                                                                                     <  2:3426194/101‑1 (MQ=11)
                      gtgcgtggcgtCCGTCGGGTCGGTGGGGTACGTGGGATCTGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAt                                                                                   <  1:4157733/91‑1 (MQ=14)
                      gtcgggcggGGCCGTGGGGTCGGTGGGGTCCGGGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAt                                                                                   <  1:2118961/100‑1 (MQ=11)
                      atcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTcgggt                                                                                   >  1:1441081/1‑99 (MQ=11)
                       tcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtc                                                                                  <  1:3304250/101‑1 (MQ=11)
                        cggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcg                                                                                 >  2:4598099/1‑101 (MQ=11)
                        cggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcg                                                                                 <  1:47992/101‑1 (MQ=14)
                        ccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtcg                                                                                 <  1:1020335/96‑1 (MQ=14)
                        ccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAtcc                                                                                 <  2:89956/99‑2 (MQ=11)
                         ggtgggtgcggTGGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtcgg                                                                                <  1:932358/91‑1 (MQ=17)
                         ggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcgg                                                                                >  2:2055254/1‑101 (MQ=11)
                         ggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGGCCGTCGGGTCCGTGGGAtcgg                                                                                >  2:3705207/1‑101 (MQ=14)
                         cgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGGTCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGCCCGTCGGAtccg                                                                                >  1:1599936/2‑99 (MQ=11)
                         cgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAtccg                                                                                <  2:849232/100‑3 (MQ=11)
                          gtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGAGCGGTCGGGTTCGCCGGGTCCGTGGGAtcggt                                                                               >  1:1670605/4‑101 (MQ=14)
                           tgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGCCCGTGGGAtcggtc                                                                              >  2:3967698/3‑101 (MQ=14)
                            gggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtcggtcg                                                                             >  1:4400442/2‑101 (MQ=17)
                            cggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAtccgtgg                                                                             <  2:3803088/101‑3 (MQ=11)
                             gggtcggTGGGGTCCATCGGGTCCGTGGGATCGGTCGGCTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtcggtcgg                                                                            <  2:2767931/95‑1 (MQ=17)
                              ggtCCTTGGGATCTGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcggtcggg                                                                           <  2:4034581/99‑2 (MQ=11)
                               gtCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtcgggt                                                                          <  2:139350/100‑3 (MQ=14)
                               aTCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGAt                                                                          <  2:4147944/101‑1 (MQ=11)
                                  ggtcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGACCGGTCGGAGCCg                                                                       >  2:1631666/5‑101 (MQ=17)
                                  cGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCg                                                                       <  1:1282700/101‑1 (MQ=14)
                                    taggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGtc                                                                     >  1:3803373/3‑100 (MQ=14)
                                    tGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGACCCGTCGGATCCGTGGGGCCCGTGGGAtcggtcgggcccttg                                                                     >  2:4491453/1‑94 (MQ=11)
                                        ggccgtggggTCCGGGGGATCGGGCGGGGCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTTGGGTCCGTGGGATCGGTCGGATCCGTGGGAt                                                                 <  1:4477416/94‑1 (MQ=18)
                                         tcggtcggGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGCCCTGGGGACCGGTCGGGTCCGTGGGAtc                                                                >  2:421017/1‑101 (MQ=11)
                                          cggtcggGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcg                                                               <  1:3970252/101‑1 (MQ=17)
                                           ggtcggGGCTGTGGGATCGGTCGTATCCGTGGGATCGGTGGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcgg                                                              <  1:2193560/101‑1 (MQ=17)
                                            gtcggGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggt                                                             >  2:1934282/1‑101 (MQ=17)
                                            gtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCAGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcggt                                                             <  1:365227/101‑1 (MQ=14)
                                             tggggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggtc                                                            <  1:3524548/99‑1 (MQ=255)
                                              ggggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggtcg                                                           >  1:871908/2‑101 (MQ=255)
                                               gggTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcggtcgg                                                          <  2:1440954/101‑1 (MQ=14)
                                                ggTCCGTGGGATCGGTGGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCggg                                                         <  1:5046256/101‑1 (MQ=255)
                                                ggTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCggg                                                         >  1:3391734/1‑101 (MQ=17)
                                                ggTCCGGGGTATCGGTCGGGTCCGTTGGAGCGGCCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCggg                                                         <  2:125068/101‑1 (MQ=18)
                                                 gTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGGCCGTCGGGTCGGTCgggg                                                        >  2:3532677/1‑100 (MQ=14)
                                                   ccGTGGGATCGGTCGGGTCCGTGGGGTCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCAGGTcc                                                      >  2:628605/1‑101 (MQ=11)
                                                    cGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCg                                                     >  2:4382459/1‑101 (MQ=11)
                                                    cGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGCCCGTAGGATCGGTCGGGTCCg                                                     >  2:2530639/1‑101 (MQ=11)
                                                    cGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCg                                                     <  2:3887955/101‑1 (MQ=17)
                                                     gTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGt                                                    >  1:1382116/1‑101 (MQ=255)
                                                     gTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGCTCGGTCGGGTCCGt                                                    >  2:75728/1‑101 (MQ=17)
                                                     gTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGCCGGGTCCGTGGGCTGGGTCGGGTCCGt                                                    >  2:1677027/1‑101 (MQ=17)
                                                      tGGGATCGGGCGGATCCGTGGGATCGGGCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGta                                                   <  1:3121764/101‑2 (MQ=17)
                                                       gggATCGGTCGGATCCTGGGGATCGGTCGGCTCCGTGGGATCGGTCGGATCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGtag                                                  <  2:2107487/101‑3 (MQ=17)
                                                       gggATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCGGAGGGGTGGGTgg                                                  >  2:1318567/1‑101 (MQ=14)
                                                       gggAGCGGTCGGGTCCGTGGGAGCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgg                                                  <  2:2381782/101‑1 (MQ=255)
                                                       cggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgg                                                  >  2:1534303/2‑101 (MQ=17)
                                                        gggtctgtcggATCCGTGGGGTCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTggg                                                 <  1:2487071/95‑1 (MQ=14)
                                                        gggtcggtcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTggg                                                 <  1:4217339/98‑1 (MQ=14)
                                                         gATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgggg                                                <  2:4887205/101‑1 (MQ=255)
                                                          aTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggat                                               >  1:3739933/1‑99 (MQ=18)
                                                            gggtcggATGCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcg                                             <  1:2791326/100‑5 (MQ=11)
                                                            cggtcggGTGCGTGTGGTCGGTCGGGGCCGTTGGATCGGTCGGGTCTGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtg                                             <  2:552134/101‑1 (MQ=17)
                                                             ggtcggGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtgg                                            >  2:2613159/1‑101 (MQ=255)
                                                             ggtcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCGGGGGGAGCGGTGGGGTCCGTCGGGTCGGTGGGGTTCGGgggatacg                                            >  2:4666900/1‑96 (MQ=9)
                                                              gtcggGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtggt                                           >  2:2930897/1‑101 (MQ=255)
                                                               tgggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcggtc                                          <  1:604803/99‑8 (MQ=11)
                                                                 gggtCCGTGGGATCGGTCGGGTCCGTAGGGTCGGTTTGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGCGACCGGTCGGGCCCACGGGGCTGGGggg                                        >  2:2839076/4‑101 (MQ=18)
                                                                      cGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTTGGGTCCGTGGGATCGGTCGGGTCCGTGTGATCGGGCAGGTCCGTAGGACCGGTcggggccg                                   >  1:2001046/1‑96 (MQ=2)
                                                                         gggATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcggtcg                                >  2:1023297/1‑101 (MQ=255)
                                                                          ggctcggttggCGCCGAAGGAGCGGGGGGCTCCGTCGGGGCCGTGGGACCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcggtcgg                               <  1:4554014/98‑1 (MQ=18)
                                                                           gATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGa                              >  2:1186658/1‑101 (MQ=255)
                                                                                   gggTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTc                      >  1:4762487/1‑101 (MQ=255)
                                                                                    ggTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcg                     >  1:499560/1‑101 (MQ=255)
                                                                                            ggATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCgccgccgcc             <  1:3260824/101‑1 (MQ=255)
                                                                                            ggATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCAGGTGGGATCGGTCGGACCGCTGTCgccgccgcc             >  1:4295853/1‑101 (MQ=255)
                                                                                              aTCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCgg           >  1:4104958/1‑101 (MQ=255)
                                                                                                 ggtcggGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACa        >  1:2358980/1‑101 (MQ=255)
                                                                                                   gcgggtccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGa      <  1:4042313/100‑1 (MQ=255)
                                                                                                     gggtccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGAcc    >  1:1860103/1‑101 (MQ=255)
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TGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCA  >  NC_014034/221972‑222175

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG