Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 222,108 G→C 13.8% P183P (CCC→CCG)  RCAP_rcc00185 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034222,1080G→C13.8% 97.6 ‑2.0 ‑1.3316P183P (CCC→CCG) RCAP_rcc00185hypothetical protein
Reads supporting (aligned to +/- strand):  new base (16/26):  ref base (137/127):  total (159/157)
Fisher's exact test strand distribution p-value = 1.34e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.22e-02

Only 100 of 396 total aligned reads displayed.

TGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGACCGGCCGCAGCCCAGCCCAGCG  >  NC_014034/222008‑222208
                                                                                                    |                                                                                                    
 cggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGGCCGTCGGGGCCGTGGGATCGGGCGGATCCGGgg                                                                                                     >  1:2696781/2‑101 (MQ=17)
 aggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTGGGGACCGTgg                                                                                                     >  2:2102809/2‑101 (MQ=14)
  gtgtcggtcggTTCCGGGTGATCGGTCGGATCCGTGGTATCGGTCGGGCCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTggg                                                                                                    <  2:319488/98‑1 (MQ=14)
  ggttcggtcggCTCCGTGGGATCTGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTggg                                                                                                    <  2:3529447/98‑1 (MQ=14)
  ggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTcgg                                                                                                    >  1:4202411/1‑101 (MQ=14)
   ggtccggggggTCCGGGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGa                                                                                                   <  1:1328632/93‑1 (MQ=18)
      cggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcg                                                                                                >  1:3836661/1‑101 (MQ=14)
      cggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGGCGGGTCCGTCGGAtcg                                                                                                >  1:2316991/1‑101 (MQ=14)
      ccggggggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcg                                                                                                <  1:3967990/96‑1 (MQ=255)
        gtcggGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggt                                                                                              <  1:2798854/101‑1 (MQ=17)
         tggggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGCGGGAtcggcc                                                                                             >  2:4053506/3‑99 (MQ=255)
           gggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcgggcgg                                                                                           >  2:3021479/1‑101 (MQ=255)
           gggTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcggtcgg                                                                                           <  2:1440954/101‑1 (MQ=14)
             gTCCGTTGGTGCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGt                                                                                         <  2:3257520/101‑1 (MQ=17)
             gTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGt                                                                                         <  1:4569042/101‑1 (MQ=255)
              tcggTGGGTTCGGCCGGATCCGGGGGAGCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTc                                                                                        <  1:3788518/98‑1 (MQ=17)
              tCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTc                                                                                        <  1:4221573/101‑1 (MQ=17)
               ccGTGGGATCGGTCGGGTCCGTGGGGTCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCAGGTcc                                                                                       >  2:628605/1‑101 (MQ=11)
               ccGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTcc                                                                                       >  1:4879052/1‑101 (MQ=255)
               ccGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTcc                                                                                       <  2:4233825/101‑1 (MQ=14)
                cGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGCCCGTAGGATCGGTCGGGTCCg                                                                                      >  2:2530639/1‑101 (MQ=11)
                 gtcggATCGGTCGGATCCGTGGGATCGGTCGGGGCCGTCGGATCCGTGGGGCCCGTCGGGTCGGGGGGATCGGTCGGGGCCGAGGGGTCGGGTGGGtgccg                                                                                     >  2:3774634/4‑97 (MQ=9)
                 gTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCgg                                                                                     >  2:1246292/1‑100 (MQ=255)
                 gTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGt                                                                                     <  1:1712233/101‑1 (MQ=255)
                 gTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGt                                                                                     >  1:1259457/1‑101 (MQ=1)
                 gTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGCTCGGTCGGGTCCGt                                                                                     >  2:75728/1‑101 (MQ=17)
                 gTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGt                                                                                     >  1:4791179/1‑101 (MQ=17)
                 gTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGCCGGGTCCGTGGGCTGGGTCGGGTCCGt                                                                                     >  2:1677027/1‑101 (MQ=17)
                  tGGGATCGGGCGGATCCGTGGGATCGGGCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGta                                                                                    <  1:3121764/101‑2 (MQ=17)
                   gggATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGGgg                                                                                   >  1:93717/1‑101 (MQ=255)
                   gggATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGGCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgg                                                                                   >  1:746176/1‑101 (MQ=255)
                   gggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAACGGTCGGGTCCGTGGGATCGGTCGGGTCCGtag                                                                                   <  1:1634497/101‑3 (MQ=17)
                   cggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgg                                                                                   >  2:1534303/2‑101 (MQ=17)
                   cggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgg                                                                                   >  2:4435467/2‑101 (MQ=17)
                    ggATCGGTCGGATCCGTGGGATCGGGCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTggg                                                                                  <  1:623079/101‑1 (MQ=17)
                     gATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgggg                                                                                 <  2:4887205/101‑1 (MQ=255)
                     gATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATGGGTCGGGTCCGTagga                                                                                 >  1:460757/1‑100 (MQ=17)
                     gATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTggga                                                                                 <  1:2631175/101‑2 (MQ=17)
                      aTCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGCCCGTGGGATCGGTCGGATCCGTGGGATCGGGCGGGTCCGGGGGGc                                                                                >  2:4548588/1‑101 (MQ=18)
                      aTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATAGGTCGGGTCCGTaggat                                                                                >  2:3836478/1‑99 (MQ=17)
                      aTCCGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggat                                                                                <  2:276367/101‑3 (MQ=14)
                        cggtcggGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtg                                                                              >  2:2947973/1‑101 (MQ=255)
                        cggtcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcg                                                                              <  1:4004815/101‑5 (MQ=14)
                         ggtcgtATCCTTAGGATCAGCAAGTTCCGGTGGATCGGGCCGATCCGTCGGGTCCGTGGAACCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcgg                                                                             <  2:837586/101‑6 (MQ=2)
                         ggtcggGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCCGGTCCGTGGGGCtgg                                                                             >  2:4199370/1‑101 (MQ=255)
                          gtcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGGTCGGTCGGGCCCGTgggatcggt                                                                            >  2:12768/1‑95 (MQ=17)
                            cggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcggtcg                                                                          >  1:2381946/1‑93 (MQ=14)
                             gggtCCGTGGGATCGGTCGGGTCCGTAGGGTCGGTTTGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGCGACCGGTCGGGCCCACGGGGCTGGGggg                                                                         >  2:2839076/4‑101 (MQ=18)
                             ggATCCGTGGGATCGGTGGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcggtcgg                                                                         <  2:26102/101‑10 (MQ=14)
                             ggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcggtcgg                                                                         >  1:4413193/1‑92 (MQ=14)
                             ggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcggtcgg                                                                         >  2:3318732/1‑92 (MQ=14)
                             ggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcggtcgg                                                                         >  1:2480382/1‑92 (MQ=14)
                             ggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCGGGGGGGTCGGGCGGGTCCATggggtcggttgc                                                                         >  2:1411640/1‑93 (MQ=14)
                               aTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTggggc                                                                       >  2:132272/1‑99 (MQ=14)
                                  cGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTTGGGTCCGTGGGATCGGTCGGGTCCGTGTGATCGGGCAGGTCCGTAGGACCGGTcggggccg                                                                    >  1:2001046/1‑96 (MQ=2)
                                    tGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcggtc                                                                  >  2:4945823/1‑101 (MQ=255)
                                     gggATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcggtcg                                                                 >  2:1023297/1‑101 (MQ=255)
                                      ggATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcggtcgg                                                                >  2:3573102/1‑101 (MQ=255)
                                      ggATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcggtcgg                                                                <  2:3927051/101‑1 (MQ=255)
                                       gATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGa                                                               >  2:1186658/1‑101 (MQ=255)
                                          cggtcggGGCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCg                                                            <  2:927204/101‑1 (MQ=255)
                                           ggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGc                                                           >  2:3878635/1‑101 (MQ=255)
                                           ggtcggGTCCGTAGGATCGGTCGGGTCCGTCCGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGc                                                           >  1:4500674/1‑101 (MQ=255)
                                            gtcggGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGAACGCt                                                          >  2:4358931/1‑101 (MQ=255)
                                               gggTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTc                                                       >  2:878957/1‑101 (MQ=255)
                                               gggTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTc                                                       <  1:4058900/101‑1 (MQ=255)
                                                gatCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTGGGGTCCGTGGGGGCGGTGGGGCCGGGGGGGCCGGGCGGCACGCTGGcg                                                      >  2:3458846/3‑101 (MQ=2)
                                                        ggATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTAGGACCGCTGTCgccgccgcc                                              >  1:357260/1‑101 (MQ=255)
                                                        ggATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCAGGTGGGATCGGTCGGACCGCTGTCgccgccgcc                                              >  1:4295853/1‑101 (MQ=255)
                                                           tcggtcggGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGa                                           >  1:1430111/1‑101 (MQ=255)
                                                              gtcgggtccggcgggtccGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAg                                        <  1:3314443/101‑1 (MQ=255)
                                                                 gggtccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGCCAGAcc                                     >  2:2475785/1‑101 (MQ=255)
                                                                 gggtccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGAcc                                     <  1:3461163/101‑1 (MQ=255)
                                                                  ggtccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGAccc                                    >  2:2182959/1‑101 (MQ=255)
                                                                   gtccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCa                                   <  1:523080/101‑1 (MQ=255)
                                                                       gtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAggcc                               >  1:1886069/1‑101 (MQ=255)
                                                                       gtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAggcc                               <  1:390075/101‑1 (MQ=255)
                                                                         cgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAggccgg                             >  2:2414225/1‑101 (MQ=255)
                                                                            gtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAggccggcaa                          <  2:2696596/101‑3 (MQ=255)
                                                                            gtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTAGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCa                          >  2:467208/1‑101 (MQ=255)
                                                                             tccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAg                         <  1:4695431/101‑1 (MQ=255)
                                                                               cgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGAc                       >  1:5030597/1‑101 (MQ=255)
                                                                                gtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGAcc                      >  2:547370/1‑101 (MQ=255)
                                                                                gtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGAcc                      <  2:504558/101‑1 (MQ=255)
                                                                                gtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGAcc                      >  1:3240606/1‑101 (MQ=255)
                                                                                gtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGAcc                      >  1:2184033/1‑101 (MQ=255)
                                                                                  gggATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGACCgg                    <  1:3678616/101‑1 (MQ=255)
                                                                                     gtcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGACCGGCCg                 <  1:2953399/100‑1 (MQ=255)
                                                                                      tcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGACCGGCCGc                <  2:1896214/101‑1 (MQ=255)
                                                                                         gtcggATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGACCGGCCGcagc             >  1:1034204/1‑101 (MQ=255)
                                                                                            ggATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGACCGGCCGcagccca          >  1:1197899/1‑101 (MQ=255)
                                                                                              aTCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGACCGGCCGcagcccagc        <  1:845973/101‑1 (MQ=255)
                                                                                               tCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGACCGGCCGcagcccagcc       <  2:40920/101‑1 (MQ=255)
                                                                                                ccGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGACCGGCCGCagcccagccc      <  2:4762164/101‑1 (MQ=255)
                                                                                                ccGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGACCGGCCGCagcccagccc      >  2:2011988/1‑101 (MQ=255)
                                                                                                  gggggATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGACCGGCCGCAGcccagcccag    <  2:4500354/99‑1 (MQ=255)
                                                                                                   tGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGACCGGCCGCAGCccagcccagc   <  1:2084970/101‑1 (MQ=255)
                                                                                                   tGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGACCGGCCGCAGCccagcccagc   <  2:1635701/101‑1 (MQ=255)
                                                                                                   tGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGACCGGCCGCAGCccagcccagc   <  1:1514186/101‑1 (MQ=255)
                                                                                                    gggATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGACCGGCCGCAGCCCAGCCCAGCg  >  2:688425/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
TGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGACCGGCCGCAGCCCAGCCCAGCG  >  NC_014034/222008‑222208

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG