Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,232,209 T→C 100% T767A (ACC→GCC)  RCAP_rcc02068 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,232,2090T→C100.0% 849.3 228T767A (ACC→GCC) RCAP_rcc02068ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (118/110):  ref base (0/0):  total (118/110)

Only 100 of 232 total aligned reads displayed.

CCGATCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGTGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAGCGCCGC  >  NC_014034/2232112‑2232305
                                                                                                 |                                                                                                
  gctCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCt                                                                                             <  2:2457826/99‑1 (MQ=255)
  gATCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCt                                                                                             <  1:4959248/101‑1 (MQ=255)
  gATCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCt                                                                                             >  2:255716/1‑101 (MQ=255)
    tCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTgg                                                                                           >  1:3569830/1‑101 (MQ=255)
      tGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGt                                                                                         >  2:4623958/1‑101 (MQ=255)
       gggCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTc                                                                                        >  2:3093574/1‑101 (MQ=255)
       gggCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTc                                                                                        >  1:3263348/1‑101 (MQ=255)
        ggCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCg                                                                                       <  2:4434350/101‑1 (MQ=255)
        ggCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCg                                                                                       <  2:1791962/101‑1 (MQ=255)
        ggCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGAGCGGCGATCTGGGTCg                                                                                       >  2:628212/1‑101 (MQ=255)
            ttccgatctgTCGCCCCCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCt                                                                                   <  1:1066317/92‑1 (MQ=38)
            gTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCt                                                                                   >  1:272869/1‑101 (MQ=255)
             tCGACAGCGTCGCCCCCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTc                                                                                  <  1:556460/101‑1 (MQ=255)
             tCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTc                                                                                  <  1:4107233/101‑1 (MQ=255)
             tCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTc                                                                                  <  2:2318275/101‑1 (MQ=255)
             gcGACAGCGTCGCCCCCTGCGTGGCGGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTc                                                                                  <  2:3149607/100‑1 (MQ=255)
              cGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGCTCTGGGTCGAGCTCa                                                                                 >  1:4400384/1‑101 (MQ=255)
              cGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCa                                                                                 <  1:1519279/101‑1 (MQ=255)
              cGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCa                                                                                 <  2:3221132/101‑1 (MQ=255)
              cGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCa                                                                                 >  2:4362858/1‑101 (MQ=255)
              cGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCa                                                                                 <  1:255750/101‑1 (MQ=255)
               gACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAg                                                                                >  2:4267977/1‑101 (MQ=255)
               gACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAg                                                                                >  2:3972760/1‑101 (MQ=255)
                aCAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGc                                                                               >  2:725007/1‑101 (MQ=255)
                  aGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGccc                                                                             >  1:1215646/1‑101 (MQ=255)
                   gCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGcccc                                                                            <  2:3949447/101‑1 (MQ=255)
                   gCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGcccc                                                                            >  1:1175745/1‑101 (MQ=255)
                     gTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCtagatcggaa                                                                          >  2:1066204/1‑92 (MQ=38)
                     gTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCtt                                                                          <  2:4851020/101‑1 (MQ=255)
                      tCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTg                                                                         >  1:3831418/1‑101 (MQ=255)
                      tCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTg                                                                         <  2:504757/101‑1 (MQ=255)
                      tCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGACGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGACGCGCTCAGCCCCTTg                                                                         >  2:287356/1‑101 (MQ=38)
                       cGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGCGCTCAGCCCCTTGa                                                                        >  1:2323400/1‑101 (MQ=255)
                        gCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGAt                                                                       >  1:2670859/1‑101 (MQ=255)
                        gCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGAt                                                                       >  1:1927051/1‑101 (MQ=255)
                        gCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGAt                                                                       >  1:2780223/1‑101 (MQ=255)
                              tGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGc                                                                 >  2:3636319/1‑101 (MQ=255)
                               gCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGcc                                                                <  2:2748893/101‑1 (MQ=255)
                               gCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGcc                                                                >  1:613913/1‑101 (MQ=255)
                               gCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGCCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGcc                                                                <  1:4296230/101‑1 (MQ=255)
                                cGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCg                                                               >  1:3175889/1‑101 (MQ=255)
                                 gTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGt                                                              >  2:4524959/1‑101 (MQ=255)
                                  gggCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTc                                                             <  1:1373622/100‑1 (MQ=255)
                                   ggCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCg                                                            <  2:174878/101‑1 (MQ=255)
                                      cGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCgaga                                                         <  2:1638582/101‑1 (MQ=255)
                                      cGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCgaga                                                         >  1:4208924/1‑101 (MQ=255)
                                              tCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCgcggcg                                                 <  1:3706267/101‑1 (MQ=255)
                                                  cgccTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAAt                                             <  1:2028914/101‑1 (MQ=255)
                                                  cgccTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAAt                                             >  1:197921/1‑101 (MQ=255)
                                                  cgccTGCGCCGTGGTCAGGGCGTCGAGCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAAt                                             <  2:1189767/101‑1 (MQ=255)
                                                   gccTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATc                                            >  2:4966830/1‑101 (MQ=255)
                                                    ccTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATcc                                           <  1:84989/101‑1 (MQ=255)
                                                      tGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATccgc                                         <  1:1000894/101‑1 (MQ=255)
                                                          ccGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTc                                     <  2:3572960/101‑1 (MQ=255)
                                                          ccGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTc                                     <  1:4063409/101‑1 (MQ=255)
                                                           cGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCt                                    <  2:2558621/101‑1 (MQ=255)
                                                           cGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCt                                    <  1:171883/101‑1 (MQ=255)
                                                            gTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGATAGCGCGGCGAAATCAGCCGTCtc                                   >  2:2512710/1‑100 (MQ=255)
                                                            gTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTg                                   >  1:561649/1‑101 (MQ=255)
                                                              ggTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCa                                 >  2:1566570/1‑101 (MQ=255)
                                                              ggTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCa                                 >  2:280746/1‑101 (MQ=255)
                                                              ggTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCa                                 <  1:2474111/101‑1 (MQ=255)
                                                                tCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCTAAATCGGCCGTCTGCAgc                               >  2:1528449/1‑101 (MQ=255)
                                                                tCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAgc                               >  1:3739475/1‑101 (MQ=255)
                                                                  aGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAgcgc                             >  1:3043923/1‑101 (MQ=255)
                                                                    ggCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCgg                           <  2:4663330/101‑1 (MQ=255)
                                                                    ggCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCgg                           <  2:1368791/101‑1 (MQ=255)
                                                                     gCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGga                          <  2:4712136/101‑1 (MQ=255)
                                                                      cGTCGATCTGCCCCGTGCTCATCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGgag                         >  1:2877691/1‑101 (MQ=255)
                                                                      cGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCCGCAGCGCGgag                         <  1:4333947/101‑1 (MQ=255)
                                                                       gTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGgaga                        >  2:2242495/1‑101 (MQ=255)
                                                                        tCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGAt                       <  1:3976344/101‑1 (MQ=255)
                                                                         cGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATc                      <  1:4669945/101‑1 (MQ=255)
                                                                         cGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATc                      >  1:767386/1‑101 (MQ=255)
                                                                         cGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATc                      <  2:3841712/101‑1 (MQ=255)
                                                                         cGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGCAGATc                      >  2:830173/1‑101 (MQ=255)
                                                                          gATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCt                     <  1:3368665/101‑1 (MQ=255)
                                                                          gATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCt                     >  1:3898544/1‑101 (MQ=255)
                                                                          gATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCt                     <  2:1252773/101‑1 (MQ=255)
                                                                           aTCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTg                    >  2:2041076/1‑101 (MQ=255)
                                                                           aTCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTg                    <  2:1994526/101‑1 (MQ=255)
                                                                             cTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTgcg                  <  1:1447723/101‑1 (MQ=255)
                                                                             cTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTgcg                  >  2:3260564/1‑101 (MQ=255)
                                                                              tGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTgcgc                 >  2:3333908/1‑101 (MQ=255)
                                                                               gCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGcc                >  2:2597463/1‑101 (MQ=255)
                                                                                 cccGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGt              <  1:988004/101‑1 (MQ=255)
                                                                                  ccGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGAGCGGAGATCTGGGCCGTg             >  2:3342376/1‑101 (MQ=255)
                                                                                    gTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCt           <  2:3732688/101‑1 (MQ=255)
                                                                                    gTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCt           <  1:3080685/101‑1 (MQ=255)
                                                                                    gTGCTCAGCGCGGCGATCTGGGTAGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCt           >  2:269838/1‑101 (MQ=255)
                                                                                     tGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTg          >  1:2589544/1‑101 (MQ=255)
                                                                                     tGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTg          <  2:1926914/101‑1 (MQ=255)
                                                                                      gCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGa         <  1:4537568/101‑1 (MQ=255)
                                                                                      gCTCAGCGCGGCGATCTGGGTCGAGCGCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGa         <  2:4188612/101‑1 (MQ=255)
                                                                                        tCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAgc       <  1:4859783/101‑1 (MQ=255)
                                                                                        tCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAgc       >  1:216715/1‑101 (MQ=255)
                                                                                         cAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAgcg      <  2:2089304/101‑1 (MQ=255)
                                                                                          aGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAgagc     >  2:1463678/1‑101 (MQ=255)
                                                                                           gcgcGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAGcgcc    <  2:4100580/101‑1 (MQ=255)
                                                                                             gcgGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAGcgccgc  <  2:2189882/101‑1 (MQ=255)
                                                                                                 |                                                                                                
CCGATCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGTGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAGCGCCGC  >  NC_014034/2232112‑2232305

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG