Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,334 C→A 2.1% intergenic (‑130/‑175) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3340C→A 2.1% 3.3 ‑1.0 ‑1.0241intergenic (‑130/‑175)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (122/113):  total (127/114)
Fisher's exact test strand distribution p-value = 3.73e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.04e-01

Only 100 of 266 total aligned reads displayed.

GCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTG.CCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGT  >  NC_014034/2249235‑2249433
                                                                                                            |                                                                                                         
 cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.t                                                                                                         <  2:1330604/101‑1 (MQ=255)
 cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.t                                                                                                         <  1:1417376/101‑1 (MQ=255)
   caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.ttt                                                                                                       <  1:2547202/101‑1 (MQ=255)
   caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.ttt                                                                                                       >  2:1873936/1‑101 (MQ=255)
   caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.ttt                                                                                                       <  1:3219229/101‑1 (MQ=255)
    aGATACCGCAGCTTGGGCACCCGGGGCAAGNAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTc                                                                                                      <  1:975538/101‑1 (MQ=255)
    aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTc                                                                                                      >  2:1270258/1‑101 (MQ=255)
    aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTc                                                                                                      <  1:3049482/101‑1 (MQ=255)
     gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCa                                                                                                     <  2:613945/101‑1 (MQ=255)
      aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCAt                                                                                                    <  1:3172710/101‑1 (MQ=255)
       tcccGCAGCTTGGGCCCCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATc                                                                                                   <  2:399346/99‑1 (MQ=255)
        aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCt                                                                                                  >  1:2949720/1‑101 (MQ=255)
        aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.CTTCGTCt                                                                                                  >  1:1260017/1‑101 (MQ=38)
         ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCtt                                                                                                 <  1:1092739/101‑1 (MQ=255)
          cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTg                                                                                                >  1:4236661/1‑101 (MQ=255)
          cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTg                                                                                                <  2:3337026/101‑1 (MQ=255)
           gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGc                                                                                               >  1:3509049/1‑101 (MQ=255)
           gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGc                                                                                               <  1:3890979/101‑1 (MQ=255)
             aGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCg                                                                                             <  1:1832923/101‑1 (MQ=255)
             aGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCg                                                                                             <  1:660105/101‑1 (MQ=255)
              ggttGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGa                                                                                            >  1:3356129/3‑101 (MQ=255)
               cTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGaa                                                                                           <  2:2643868/101‑1 (MQ=255)
                 tGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGaaaa                                                                                         <  2:4579861/101‑1 (MQ=255)
                  gggCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAat                                                                                        <  2:322504/101‑1 (MQ=255)
                  gggCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAat                                                                                        >  1:3198249/1‑101 (MQ=255)
                   ggCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAata                                                                                       <  1:2177755/101‑1 (MQ=255)
                    gCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAatat                                                                                      <  1:4195545/101‑1 (MQ=255)
                      aCCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATcc                                                                                    <  2:157007/101‑1 (MQ=255)
                       cccGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATccc                                                                                   >  2:3515121/1‑101 (MQ=255)
                         cGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCgg                                                                                 >  1:1218484/1‑101 (MQ=255)
                             gCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCgggggg                                                                             <  1:2842509/101‑1 (MQ=255)
                             gCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCgggggg                                                                             >  2:58101/1‑101 (MQ=255)
                              cAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGt                                                                            <  2:4790983/101‑1 (MQ=255)
                               gagaagGGGGCTGCGC.CCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGTGTg                                                                           <  2:1110866/100‑1 (MQ=38)
                               aagaagGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGG.TTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                           >  1:3824074/1‑101 (MQ=255)
                               aagaagGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                           >  2:365312/1‑101 (MQ=255)
                               aagaagGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                           >  1:308160/1‑101 (MQ=255)
                               aagaagGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                           >  1:2483520/1‑101 (MQ=255)
                                 gaagGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGTAAATATCCCGGGGGGTGaa                                                                         >  1:329222/1‑101 (MQ=255)
                                    gggggCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tgg                                                                     <  2:3118500/101‑1 (MQ=255)
                                     ggggCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                                    <  2:2296008/101‑1 (MQ=255)
                                       ggCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                                                  >  1:2439396/1‑101 (MQ=255)
                                         ct.ctGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..g                                                              <  1:1751733/101‑1 (MQ=255)
                                         ct.ctGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..g                                                              <  1:4295982/101‑1 (MQ=255)
                                            ctG.CCCTCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                            <  2:2212381/101‑1 (MQ=255)
                                              gcccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                          <  2:4133381/99‑1 (MQ=255)
                                                ccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCagag                                                        >  1:817140/1‑101 (MQ=255)
                                                    cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                                     >  1:2274477/1‑101 (MQ=255)
                                                     gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGAGAA.TGGCGCA..GCCAGAggggg                                                    >  2:1403680/1‑101 (MQ=255)
                                                      tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                   >  2:4829050/1‑101 (MQ=255)
                                                      tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                   <  1:392326/101‑1 (MQ=255)
                                                       cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                  <  1:2111314/101‑1 (MQ=255)
                                                       cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                  <  1:4980737/101‑1 (MQ=255)
                                                        ccgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAAC.CGGA..TGCATTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggg                                                     >  1:4872835/9‑101 (MQ=21)
                                                         cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                >  1:396248/1‑101 (MQ=255)
                                                         cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                <  1:675225/101‑1 (MQ=255)
                                                         cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                <  2:4307761/101‑1 (MQ=255)
                                                         cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCC.TTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAggggg                                                    >  2:1685360/8‑101 (MQ=21)
                                                          gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                               >  1:4454983/1‑101 (MQ=255)
                                                          gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                               >  1:2566735/1‑101 (MQ=255)
                                                           tGCCTGGACT.CCCCCGGGATATTTGAGGCAAGATCAG...GGGG..GGT.TTTCATCTTTCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgcg                                              <  1:2240441/101‑1 (MQ=37)
                                                              cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc                                          <  1:2591059/101‑1 (MQ=255)
                                                              cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc                                          >  1:1052733/1‑101 (MQ=255)
                                                               cggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCC.TTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAgcg                                              <  1:276066/100‑1 (MQ=18)
                                                                ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGTCAGCG.ccccc                                        <  1:4368513/101‑1 (MQ=255)
                                                                 gACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccc                                       <  1:4752675/101‑2 (MQ=255)
                                                                  aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccct                                      >  1:485248/1‑99 (MQ=255)
                                                                   cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccctt                                     <  1:4041099/101‑4 (MQ=255)
                                                                    t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttc                                    <  2:1645830/101‑5 (MQ=255)
                                                                    t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttc                                    >  1:4658426/1‑97 (MQ=255)
                                                                      cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttcg                                   <  1:2330065/101‑6 (MQ=255)
                                                                       ccccGGGATATTTGCGGCAAGGTGAA...GGGGCATGA.TTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCC.tg                                     >  1:2863925/1‑100 (MQ=2)
                                                                        cccGGGATATTTGCGGCAAGATGAA...GGGGCATGA.TTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCC.tgc                                    <  2:1204802/101‑3 (MQ=0)
                                                                           gggATATTTGCGGCAAGATGAA...GGGGCATGA.TTGCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCC.tgcccc                                 <  1:4326169/101‑6 (MQ=0)
                                                                             gATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCgcgggcgg                            >  1:4569746/1‑101 (MQ=255)
                                                                              atatTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGggcggc                           >  2:4464893/1‑101 (MQ=255)
                                                                              atatTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTAAA.TGGCGCA..GACAGAGGGGGGCAGCGCCCCCC.TTCGCGggcggc                           >  2:901926/1‑101 (MQ=37)
                                                                                atTTGCGGCAAGATGAA...GGGGCATGA.TTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCC.tgccccgccgg                            >  2:4122224/1‑91 (MQ=2)
                                                                                 tttGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCcgc                        <  2:4880746/101‑1 (MQ=255)
                                                                                  ttGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCcgcg                       >  2:1926736/1‑101 (MQ=255)
                                                                                  ttGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCcgcg                       >  2:535086/1‑101 (MQ=255)
                                                                                  ttGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCcgcg                       >  2:508930/1‑101 (MQ=255)
                                                                                    gAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGAc                     >  2:4379902/1‑101 (MQ=255)
                                                                                    gAGGAAAGATGAA...GGGG..GGC.TTTCATTTTGCCGAAAATTTCGGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGAc                     <  2:1789784/101‑1 (MQ=37)
                                                                                      ggCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGAcgc                   <  2:3458686/101‑1 (MQ=255)
                                                                                         aaGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGAcgcgcg                >  2:3936860/1‑101 (MQ=255)
                                                                                           gATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCCATTCGCGGGCGGCCGCGACGCGcgcc              >  2:225834/1‑101 (MQ=255)
                                                                                            aTGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGcgccg             <  2:2697914/101‑1 (MQ=255)
                                                                                             tGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGcgcagc            >  2:2760495/1‑101 (MQ=255)
                                                                                              gAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCa           >  1:3437886/1‑101 (MQ=255)
                                                                                              gAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCa           >  2:2560414/1‑101 (MQ=255)
                                                                                              gAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCa           <  2:4682148/101‑1 (MQ=255)
                                                                                               aa...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCaa          >  2:1052694/1‑101 (MQ=255)
                                                                                               aa...GGGC..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCaa          >  2:3693541/1‑101 (MQ=255)
                                                                                                a...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAAc         >  2:4074801/1‑101 (MQ=255)
                                                                                                a...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAAc         <  2:2403842/101‑1 (MQ=255)
                                                                                                    gggg..ggC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACa        <  1:365365/101‑1 (MQ=255)
                                                                                                    gggg..ggC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACa        <  1:2124760/101‑1 (MQ=255)
                                                                                                     ggg..ggC.TTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAg       >  1:2062827/1‑101 (MQ=255)
                                                                                                            c.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGt  <  2:3826913/101‑1 (MQ=255)
                                                                                                            |                                                                                                         
GCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTG.CCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGT  >  NC_014034/2249235‑2249433

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG