Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,347 G→C 6.6% intergenic (‑143/‑162) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3470G→C 6.6% 27.0 0.0 ‑0.6226intergenic (‑143/‑162)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (8/7):  ref base (117/93):  total (125/101)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.32e-01

Only 100 of 254 total aligned reads displayed.

AGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTG.CCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAT  >  NC_014034/2249248‑2249446
                                                                                                             |                                                                                                        
aGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCg                                                                                                          <  2:1443510/101‑1 (MQ=255)
 gCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGa                                                                                                         >  1:1296416/1‑101 (MQ=255)
 gCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGa                                                                                                         <  2:4576435/101‑1 (MQ=255)
   ttGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGaaa                                                                                                       >  2:1220639/1‑101 (MQ=255)
    tGGGCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGG.TTTCATCTTGCCGaaaa                                                                                                      >  2:4819187/1‑101 (MQ=255)
     gggCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCATGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAat                                                                                                     <  1:4425801/101‑1 (MQ=255)
      ggCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAata                                                                                                    <  1:2177755/101‑1 (MQ=255)
       gCACCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAatat                                                                                                   <  1:4195545/101‑1 (MQ=255)
         aCCCGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATcc                                                                                                 <  1:431164/101‑1 (MQ=255)
          cccGGGGCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATccc                                                                                                <  1:3188297/101‑1 (MQ=255)
          cactcggCAAGAAGCGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATacc                                                                                                >  2:3212936/6‑101 (MQ=37)
              gggCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCgggg                                                                                            >  2:1044724/1‑101 (MQ=255)
                gCAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCgggggg                                                                                          >  2:58101/1‑101 (MQ=255)
                 cAAGAAGGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGt                                                                                         <  2:4790983/101‑1 (MQ=255)
                  gagaagGGGGCTGCGC.CCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGTGTg                                                                                        <  2:1110866/100‑1 (MQ=38)
                  aagaagGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGG.TTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                                        >  1:3824074/1‑101 (MQ=255)
                  aagaagGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                                        <  1:4613386/101‑1 (MQ=255)
                  aagaagGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                                        >  1:308160/1‑101 (MQ=255)
                  aagaagGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATAATTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                                        >  2:4368196/1‑101 (MQ=255)
                    gaagGGGGCT.CTGCCCC.CGTCCCGTGCCTGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCTAAAATATCCCGGGGGGTGaa                                                                                      <  1:4724646/101‑1 (MQ=37)
                    gaagGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGTAAATATCCCGGGGGGTGaa                                                                                      >  1:329222/1‑101 (MQ=255)
                     aagGGGGCT.CTGCCCC.CGTGCCGTGCGGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                                    <  2:1997524/101‑1 (MQ=37)
                     aagGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                                    <  2:3508779/101‑1 (MQ=255)
                     aagGCGGCT.CGGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                                    <  2:673696/101‑1 (MQ=38)
                      agGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                                   <  1:4281775/101‑1 (MQ=255)
                      agGGGGCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                                   >  1:25854/1‑101 (MQ=255)
                       gggggCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tgg                                                                                  <  2:3118500/101‑1 (MQ=255)
                         gggCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcg                                                                                <  2:4028024/101‑1 (MQ=255)
                          ggCT.CTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGCGAA.TGgcgc                                                                               >  1:1234036/1‑101 (MQ=255)
                            ct.ctGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..g                                                                           <  1:1751733/101‑1 (MQ=255)
                              tctgcccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gc                                                                          <  2:2262889/96‑1 (MQ=255)
                               ctgcccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                                         <  2:4878389/97‑1 (MQ=255)
                               ctgcccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..Gcc                                                                         >  1:3458951/5‑101 (MQ=255)
                               ctgcccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..Gcc                                                                         >  1:3524313/5‑101 (MQ=255)
                               ctG.CCCTCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                                         <  2:2212381/101‑1 (MQ=255)
                                 gcccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                       <  1:1942502/99‑1 (MQ=255)
                                 gcccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                       >  1:3994163/3‑101 (MQ=255)
                                 gcccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                       <  2:4133381/99‑1 (MQ=255)
                                 gcccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGAAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATACCGGGGGGGGAA.GGGCGCA..GCCag                                                                       >  1:2491384/3‑101 (MQ=38)
                                       cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                                                  >  1:2776911/1‑101 (MQ=255)
                                       cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                                                  >  1:2274477/1‑101 (MQ=255)
                                        gtcccgtGCCGGGACT.CCCCCGGGTTATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                                 >  2:3670845/1‑101 (MQ=255)
                                        gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                                 >  1:3213276/1‑101 (MQ=255)
                                         tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                                <  2:4118106/101‑1 (MQ=255)
                                          cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                               <  1:2111314/101‑1 (MQ=255)
                                          cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                               <  1:4980737/101‑1 (MQ=255)
                                           ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                                              <  2:3319237/101‑1 (MQ=255)
                                           ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GACAGAGGGGGGCa                                                              <  1:2728576/101‑1 (MQ=255)
                                           ccgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAAC.CGGA..TGCATTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggg                                                                  >  1:4872835/9‑101 (MQ=21)
                                            cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                             >  1:396248/1‑101 (MQ=255)
                                            cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                             <  1:3904232/101‑1 (MQ=255)
                                            cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCC.TTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAggggg                                                                 >  2:2381758/8‑101 (MQ=18)
                                             gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                                            >  1:4454983/1‑101 (MQ=255)
                                              caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCC.TTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGc                                                               <  2:902873/96‑1 (MQ=18)
                                               gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgcg.c                                                         >  2:2661997/1‑101 (MQ=255)
                                               gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.GGGCGCA..GCCAGAGGGGGGCAgag.c                                                         >  2:1737242/1‑101 (MQ=255)
                                                 gcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCC.TTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAgc                                                            <  1:2459501/99‑1 (MQ=18)
                                                 cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc                                                       >  1:1052733/1‑101 (MQ=255)
                                                   ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCC.TTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAgcg.c                                                         <  2:3846146/101‑1 (MQ=17)
                                                     ccT.CCCCAAGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc.ccct                                                  <  2:3823789/100‑3 (MQ=255)
                                                     aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc.ccct                                                  >  1:2140057/1‑99 (MQ=255)
                                                       t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc.cccttc                                                <  2:1645830/101‑5 (MQ=255)
                                                         cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc.cccttcg                                               <  1:2215211/101‑6 (MQ=255)
                                                         cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc.cccttcg                                               <  1:2330065/101‑6 (MQ=255)
                                                          ccccGGGATATTTGCGGCAAGGTGAA...GGGGCATGA.TTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCC.CCtg                                                  >  1:2863925/1‑100 (MQ=2)
                                                          ccccGGGATATTTGCGGCAAGATGAA...GGGGCATGA.TTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCC.CCtg                                                  >  1:3435718/1‑100 (MQ=0)
                                                          ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTcgc                                               <  2:4298079/101‑1 (MQ=255)
                                                              gggATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCgcgggc                                           <  1:654632/101‑1 (MQ=255)
                                                               ggATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCgcggggg                                          >  1:3003233/1‑99 (MQ=255)
                                                                 atatTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGggcggc                                        >  2:4464893/1‑101 (MQ=255)
                                                                 atatTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGAAGCGCCCC.CCTTCCGGggcggc                                        >  1:1142867/1‑101 (MQ=25)
                                                                 atatTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGCGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGggcggc                                        >  1:74012/1‑101 (MQ=255)
                                                                  tatTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGcc                                       <  2:2757136/101‑1 (MQ=255)
                                                                   atTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCcg                                      >  2:1375132/1‑101 (MQ=255)
                                                                     ttGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGCGGGGGGCAGCGCCCC.CCTTAGCGGGCGGCcgcg                                    >  1:4345644/1‑101 (MQ=37)
                                                                     ttGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCcgcg                                    >  2:535086/1‑101 (MQ=255)
                                                                      tGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGa                                   <  1:3864265/101‑1 (MQ=255)
                                                                        aggtaggaggAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGAcg                                 <  2:1582857/92‑1 (MQ=25)
                                                                         ggCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGAcgc                                <  2:3458686/101‑1 (MQ=255)
                                                                          gCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGAcgcg                               <  2:4658103/101‑1 (MQ=255)
                                                                           cAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCACCTTCGCGGGCGGCCGCGAcgcgc                              >  2:1280495/1‑101 (MQ=255)
                                                                               aTGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGcgccg                          <  2:2697914/101‑1 (MQ=255)
                                                                                 gAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCa                        >  2:3637792/1‑101 (MQ=255)
                                                                                 gAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCa                        >  2:2560414/1‑101 (MQ=255)
                                                                                 gAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCa                        >  2:515019/1‑101 (MQ=255)
                                                                                  aa...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCGGCaa                       >  1:2565231/1‑101 (MQ=255)
                                                                                   a...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                      <  1:2855191/101‑1 (MQ=255)
                                                                                   a...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                      >  2:1840746/1‑101 (MQ=255)
                                                                                   a...GGGG..GGC.TTTCATCTTGCCAAAAATGTCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                      <  2:396183/101‑1 (MQ=37)
                                                                                       gggg..ggC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                     <  1:4806444/101‑1 (MQ=255)
                                                                                       gggg..ggC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                     <  1:4133575/101‑1 (MQ=255)
                                                                                                 tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg              >  1:2365250/1‑101 (MQ=255)
                                                                                                   tCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcc            >  1:2075832/1‑101 (MQ=255)
                                                                                                   tCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcc            >  2:5009555/1‑101 (MQ=255)
                                                                                                   tCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcc            <  2:2483346/101‑1 (MQ=255)
                                                                                                        ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt       >  2:1786414/1‑101 (MQ=255)
                                                                                                           ccGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc    <  2:2778809/101‑1 (MQ=255)
                                                                                                            ggAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa   <  2:1013479/100‑1 (MQ=255)
                                                                                                             gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt  >  1:1794654/1‑101 (MQ=255)
                                                                                                             gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGGCGTGCCCCTTTGTCAt  >  2:1197923/1‑101 (MQ=255)
                                                                                                             |                                                                                                        
AGCTTGGGCACCCGGGGCAAGAAGGGGGCT.CTG.CCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGC.TTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAT  >  NC_014034/2249248‑2249446

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG