Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,262,586 T→C 100% Q55R (CAG→CGG)  RCAP_rcc02105 ← IS3/IS911 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,262,5860T→C100.0% 729.7 202Q55R (CAG→CGG) RCAP_rcc02105IS3/IS911 family transposase
Reads supporting (aligned to +/- strand):  new base (120/82):  ref base (0/0):  total (120/82)

Only 100 of 231 total aligned reads displayed.

TTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCGTCAGCTGCTTGGCATCGGACAAATCCATCCCACCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCG  >  NC_014034/2262489‑2262682
                                                                                                 |                                                                                                
ttGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgccgct                                                                                               >  2:4830118/1‑101 (MQ=255)
ttGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgccgct                                                                                               >  2:2477654/1‑101 (MQ=255)
ttGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgccgct                                                                                               >  1:1621796/1‑101 (MQ=255)
ttGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgccgct                                                                                               <  1:390110/101‑1 (MQ=255)
 tGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCtt                                                                                              >  1:1025932/1‑101 (MQ=255)
 tGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCtt                                                                                              >  1:1156232/1‑101 (MQ=255)
 tGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCtt                                                                                              >  2:312754/1‑101 (MQ=255)
  gggCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTg                                                                                             <  2:4407360/101‑1 (MQ=255)
  gggCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTg                                                                                             <  1:8347/101‑1 (MQ=255)
    gCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGc                                                                                           >  2:549262/1‑101 (MQ=255)
     cTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCa                                                                                          >  2:414/1‑101 (MQ=255)
      ttGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCAt                                                                                         <  1:3021928/101‑1 (MQ=255)
       tGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATc                                                                                        >  1:1492078/1‑101 (MQ=255)
       tGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATc                                                                                        >  1:5000673/1‑101 (MQ=255)
       tGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATc                                                                                        >  2:1738699/1‑101 (MQ=255)
        gAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCg                                                                                       >  2:2316238/1‑101 (MQ=255)
         aaCCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCgg                                                                                      <  1:4757212/101‑1 (MQ=255)
         aaCCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCgg                                                                                      <  2:2279925/101‑1 (MQ=255)
           ccAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGAc                                                                                    >  1:4533531/1‑101 (MQ=255)
            cAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACa                                                                                   <  2:1913878/101‑1 (MQ=255)
             aaTGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTTTTCCTCAAACTGCTTCAGCCCCTTGGCATCGGCCaa                                                                                  >  2:524965/1‑101 (MQ=18)
               tGGCGCCACACTGGTTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAat                                                                                <  1:3744656/101‑1 (MQ=255)
               tGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAat                                                                                >  2:1602220/1‑101 (MQ=255)
                ggCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatc                                                                               >  1:3691260/1‑101 (MQ=255)
                ggCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatc                                                                               >  2:924274/1‑101 (MQ=255)
                    ccACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatccatc                                                                           <  2:1816724/101‑1 (MQ=255)
                    ccACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatccatc                                                                           <  1:957853/101‑1 (MQ=255)
                    ccACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatccatc                                                                           <  2:4391411/101‑1 (MQ=255)
                     cacaCTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatccatcc                                                                          <  2:3003615/101‑1 (MQ=255)
                      acacTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATccc                                                                         <  2:3429771/101‑1 (MQ=255)
                        acTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATcccgc                                                                       <  1:3873124/101‑3 (MQ=255)
                         cTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCccgcc                                                                      <  2:2187256/101‑1 (MQ=255)
                          tGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCccgcca                                                                     <  2:1029750/101‑1 (MQ=255)
                           ggCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCAt                                                                    >  1:2161371/1‑101 (MQ=255)
                            gCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATa                                                                   <  2:882647/101‑1 (MQ=255)
                             cTCGATCCAGCATCACATCCGCCACCAGGCGCTTCGGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATAc                                                                  <  1:2474570/101‑1 (MQ=21)
                              tggttCCCGCATCCCACCCGCCACCAGGCGCTTCTGCTTGGCTTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACt                                                                 <  1:2701379/97‑1 (MQ=18)
                              tCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACt                                                                 >  1:3489694/1‑101 (MQ=255)
                               cGATCCAGCATCCCATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACtt                                                                <  2:2287346/101‑1 (MQ=18)
                               cGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACtt                                                                >  2:2079250/1‑101 (MQ=255)
                                gacccAGCACCACACCCGCCCCCAGGCGCTTCAGCTTGGCGTTCTCTCCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAGATCCATCCCGCCATACTTg                                                               <  2:3448488/98‑1 (MQ=17)
                                gATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCTTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTg                                                               <  2:5014475/101‑1 (MQ=18)
                                 aTCCAGCATCACATCCGCCCCCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTgg                                                              <  2:4646481/101‑1 (MQ=18)
                                 aTCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTgg                                                              >  1:1815429/1‑101 (MQ=255)
                                 aTCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTgg                                                              >  2:1680049/1‑101 (MQ=255)
                                 aTCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTgg                                                              >  1:4646804/1‑101 (MQ=255)
                                  tCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGc                                                             <  1:75064/101‑1 (MQ=255)
                                  tCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGc                                                             >  1:3342167/1‑101 (MQ=255)
                                     aGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCtt                                                          <  2:3577320/101‑1 (MQ=255)
                                        aTCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAg                                                       >  2:2782583/1‑101 (MQ=255)
                                          cacaTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCt                                                     >  1:4017775/1‑101 (MQ=255)
                                            cacccGCCCCCAGGCGCTTCAGCTGGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCTTCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTg                                                   <  2:3719276/98‑1 (MQ=18)
                                             aTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGt                                                  <  1:3321567/101‑1 (MQ=255)
                                              tCCGCCACCAGGCGCTTAAGCTTGGCGTACTATTCCTCATACTGCTTAAGCCGCTTGGCATAGGACAAATATATCCCGACCTACTTGGCCTTCAGCTTATa                                                 >  2:824504/1‑101 (MQ=16)
                                               ccGCCCCCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAg                                                <  1:968078/101‑1 (MQ=18)
                                                cGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGa                                               <  1:1277773/101‑1 (MQ=255)
                                                   caccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAgg                                            >  1:635608/1‑101 (MQ=255)
                                                    accaGGAGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGt                                           <  1:823519/101‑1 (MQ=18)
                                                     cgaGGCCCTTCAGTTTGGCGTTCTCTTCCTAAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTc                                          <  2:2010567/99‑1 (MQ=18)
                                                     ccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTc                                          >  1:2575591/1‑101 (MQ=255)
                                                      caGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTcg                                         >  1:3651291/1‑101 (MQ=255)
                                                           gcTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCgggg                                    >  2:717091/1‑101 (MQ=255)
                                                            cTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGc                                   >  2:242852/1‑101 (MQ=255)
                                                             ttCGGCTTGGCGTTCTCTTCCTCAAACTGCTTCGGCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCt                                  <  1:2277039/101‑1 (MQ=21)
                                                             ttCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCt                                  >  1:154072/1‑101 (MQ=255)
                                                              tCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTg                                 >  1:2796370/1‑101 (MQ=255)
                                                              tCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGCCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTg                                 <  1:1680178/101‑1 (MQ=21)
                                                               cAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGa                                >  1:4843981/1‑101 (MQ=255)
                                                               cAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGa                                >  1:411408/1‑101 (MQ=255)
                                                                aGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCAGCCATACTTGGCCTTCAGCTTGTAGAAGCTCGTGGGGCTGAg                               >  2:3792688/1‑101 (MQ=21)
                                                                  cTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGcc                             >  2:4896273/1‑101 (MQ=255)
                                                                   ttGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGccc                            >  1:4930431/1‑101 (MQ=255)
                                                                    tGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCa                           >  1:2397467/1‑101 (MQ=25)
                                                                     ggCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCAt                          >  2:2842443/1‑101 (MQ=25)
                                                                     ggCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCAt                          >  1:3294303/1‑101 (MQ=25)
                                                                      gCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATg                         <  1:2696474/101‑1 (MQ=255)
                                                                       cGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGc                        >  2:3282236/1‑101 (MQ=255)
                                                                        gTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCt                       >  2:896509/1‑101 (MQ=255)
                                                                         ttCTCTTCCACAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCtt                      >  2:1265595/1‑101 (MQ=25)
                                                                          tctcTTCCTCAACCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTc                     <  1:896614/101‑1 (MQ=25)
                                                                           ctctTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTcc                    <  1:312807/101‑1 (MQ=255)
                                                                           ctctTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTcc                    >  1:2187779/1‑101 (MQ=255)
                                                                            tctTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCg                   <  1:2316402/101‑1 (MQ=255)
                                                                            tctTCCTCAAACTGCTTCAGCCGCTTGGCATCGAACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCg                   <  1:420/101‑1 (MQ=25)
                                                                             ctTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCgg                  >  2:2659806/1‑101 (MQ=37)
                                                                              ttCCTCAACCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGc                 <  2:1491948/101‑1 (MQ=25)
                                                                              ttCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGc                 >  1:2988424/1‑101 (MQ=255)
                                                                               tCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCa                >  1:5043586/1‑101 (MQ=255)
                                                                                ccTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCaa               >  1:618814/1‑101 (MQ=255)
                                                                                    aaaCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCt           >  1:1206247/1‑101 (MQ=255)
                                                                                    aaaCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCt           >  1:2425149/1‑101 (MQ=255)
                                                                                     accTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTc          <  1:4348949/99‑1 (MQ=255)
                                                                                        tGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGa       >  2:3920449/1‑101 (MQ=255)
                                                                                        tGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGa       <  1:248608/101‑1 (MQ=255)
                                                                                         gcttCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGaa      >  2:4836974/4‑101 (MQ=255)
                                                                                         gcttCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGaa      >  1:2144037/4‑101 (MQ=255)
                                                                                           ttCAGCCGCTTGGCATCGGCCAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGt    <  1:1460526/100‑1 (MQ=255)
                                                                                           ttCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGt    >  2:4503245/2‑101 (MQ=255)
                                                                                            tCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTc   <  2:4186041/101‑1 (MQ=255)
                                                                                             cAGCCGCTGGGCATCGGACAAACCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCg  <  2:635519/101‑1 (MQ=25)
                                                                                                 |                                                                                                
TTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCGTCAGCTGCTTGGCATCGGACAAATCCATCCCACCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCG  >  NC_014034/2262489‑2262682

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG