Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,462,450 G→C 100% M103M (GTG→CTG)  RCAP_rcc02286 → divalent ion symporter family

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,462,4500G→C100.0% 754.4 201M103M (GTG→CTG) RCAP_rcc02286divalent ion symporter family
Reads supporting (aligned to +/- strand):  new base (80/121):  ref base (0/0):  total (80/121)

Only 100 of 217 total aligned reads displayed.

TGACGCGGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGGTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCGGGCTG  >  NC_014034/2462352‑2462545
                                                                                                  |                                                                                               
tGACGCGGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTg                                                                                               <  1:3910544/101‑1 (MQ=255)
 gACGCGGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGc                                                                                              <  2:3925781/101‑1 (MQ=255)
 gACGCGGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGGGGTCGGCGGGCTGc                                                                                              >  1:2303098/1‑101 (MQ=255)
  aCGCGGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCt                                                                                             <  2:4397836/101‑1 (MQ=255)
  aCGCGGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCt                                                                                             >  1:410431/1‑101 (MQ=255)
    gcgGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCttt                                                                                           >  2:1581841/1‑101 (MQ=255)
    gcgGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCttt                                                                                           <  1:3707574/101‑1 (MQ=255)
     cgGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTc                                                                                          <  1:843365/101‑1 (MQ=255)
     cgGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTc                                                                                          <  1:1780261/101‑1 (MQ=255)
     cgGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGGGGTCGGCGGGCTGCtttt                                                                                          >  1:4309828/1‑100 (MQ=255)
      ggACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCg                                                                                         <  1:669317/101‑1 (MQ=255)
      ggACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCAAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCg                                                                                         >  1:107894/1‑101 (MQ=255)
       gACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGa                                                                                        <  2:3530689/101‑1 (MQ=255)
           ggggCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGt                                                                                    <  2:3646452/101‑1 (MQ=255)
            gggCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGtg                                                                                   >  2:7858/1‑101 (MQ=255)
               cGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGtgatg                                                                                >  1:3205499/1‑101 (MQ=255)
                gATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGtgatga                                                                               >  2:2043303/1‑101 (MQ=255)
                   cAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGaaaa                                                                            <  1:5008182/101‑3 (MQ=255)
                     gcgcgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAAt                                                                          <  2:4309511/101‑1 (MQ=255)
                     gcgcgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAAt                                                                          <  2:3621875/101‑1 (MQ=255)
                      cggccTGGGGCGGCTTCTGTCGACCCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATg                                                                         <  2:4236883/97‑1 (MQ=255)
                      cgcgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATg                                                                         <  1:3647403/101‑1 (MQ=255)
                      cgcgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATg                                                                         <  2:820757/101‑1 (MQ=255)
                        cgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCGGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTc                                                                       <  1:2929010/101‑1 (MQ=255)
                        cgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTc                                                                       <  1:4120826/101‑1 (MQ=255)
                        cgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTc                                                                       >  2:1538412/1‑101 (MQ=255)
                        cgcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTc                                                                       <  2:3248645/101‑1 (MQ=255)
                         gcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCa                                                                      >  2:1004705/1‑101 (MQ=255)
                         gcTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGGGATGAACAATGTCa                                                                      >  1:195607/1‑101 (MQ=255)
                          cTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCAc                                                                     <  1:3384292/101‑1 (MQ=255)
                           tGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCAcc                                                                    <  2:3702226/101‑1 (MQ=255)
                            ggCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCTCGGCTGGGGGTGGTCGGCGGGCTGCTTTTGGGCGGGATGAACAATGTCACcg                                                                   >  1:1321898/1‑101 (MQ=255)
                            ggCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACcg                                                                   >  2:3327989/1‑101 (MQ=255)
                            ggCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACcg                                                                   <  1:2511903/101‑1 (MQ=255)
                            ggCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACcg                                                                   >  2:4621375/1‑101 (MQ=255)
                             gcgcGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACtgc                                                                  <  1:2879385/101‑1 (MQ=255)
                              cgcgGCTTCTGTCGCACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACcgcg                                                                 <  1:2109238/101‑1 (MQ=255)
                               gcgGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCgcgc                                                                >  2:1116335/1‑101 (MQ=255)
                                cgGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCt                                                               <  2:2308134/101‑1 (MQ=255)
                                cgGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCt                                                               <  1:545323/101‑1 (MQ=255)
                                   cTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGgc                                                            <  2:1728913/101‑1 (MQ=255)
                                     tCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGgcgc                                                          >  1:17099/1‑101 (MQ=255)
                                      cTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCt                                                         >  1:4958085/1‑101 (MQ=255)
                                      cTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCt                                                         >  1:4942782/1‑101 (MQ=255)
                                       tGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCCCCGCGCTGGCGCtt                                                        <  1:2245188/101‑1 (MQ=255)
                                        gTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCttt                                                       <  1:4346813/101‑1 (MQ=255)
                                        gTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCttt                                                       >  2:4202115/1‑101 (MQ=255)
                                         tCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCtttt                                                      <  2:4090217/101‑1 (MQ=255)
                                         tCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCtttt                                                      <  1:2043447/101‑1 (MQ=255)
                                         tCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCtttt                                                      <  2:4551072/101‑1 (MQ=255)
                                         tCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCtttt                                                      >  2:3809436/1‑101 (MQ=255)
                                         tCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCtttt                                                      <  1:3762227/101‑1 (MQ=255)
                                          cGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTg                                                     <  1:4532054/101‑1 (MQ=255)
                                          cGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTg                                                     >  2:2853210/1‑101 (MQ=255)
                                           gaccccGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGa                                                    <  2:4500741/98‑1 (MQ=255)
                                           gAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGa                                                    >  1:4436386/1‑101 (MQ=255)
                                           gAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGa                                                    >  2:2084009/1‑101 (MQ=255)
                                            aaCCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGAt                                                   <  1:859376/101‑1 (MQ=255)
                                             aCCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGGTg                                                  <  1:1004815/101‑1 (MQ=255)
                                             aCCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATg                                                  >  1:4291579/1‑101 (MQ=255)
                                              cccGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGc                                                 <  2:587729/101‑1 (MQ=255)
                                                cGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGccc                                               <  2:1266517/101‑1 (MQ=255)
                                                cGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGccc                                               >  2:656250/1‑101 (MQ=255)
                                                     cTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGa                                          <  1:2618274/101‑1 (MQ=255)
                                                       gCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACa                                        <  1:1876214/101‑1 (MQ=255)
                                                        ccGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACAt                                       <  2:3859459/101‑1 (MQ=255)
                                                         cGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATc                                      <  1:3088113/101‑1 (MQ=255)
                                                         cGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATc                                      <  1:1538542/101‑1 (MQ=255)
                                                         cGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATCAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATc                                      <  2:2581173/101‑1 (MQ=255)
                                                          ggTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCg                                     <  1:7866/101‑1 (MQ=255)
                                                            tCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGcc                                   <  1:81893/101‑1 (MQ=255)
                                                              aGCCTGCATGTGGCGGGGCTGGGGGGGGTCGGCGGGCTGCTTTCGGGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGCCATCGCCAc                                 >  1:3323671/1‑101 (MQ=255)
                                                              aGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCAc                                 <  1:3371587/101‑1 (MQ=255)
                                                              aGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCAc                                 <  2:1022349/101‑1 (MQ=255)
                                                               gCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACg                                <  1:1373654/101‑1 (MQ=255)
                                                                ccTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACgg                               >  1:1587077/1‑101 (MQ=255)
                                                                   gCATGTGGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGgcgc                            <  1:2825531/101‑1 (MQ=255)
                                                                       gtgGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCaa                        <  2:3946079/101‑1 (MQ=255)
                                                                       gtgGCGCGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCaa                        >  1:2386169/1‑101 (MQ=255)
                                                                       ggggCGCGGCTGGGGGTGGCCGGGGGGCTTCTTTCGAGCGTGATGAAAAATTTCACCGCCCTGGCCTTTTTGATCCCCGTCGACATCCCCACGgccccaaa                        <  2:2506515/99‑7 (MQ=255)
                                                                          gcgcGGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGc                     <  2:4942452/101‑1 (MQ=255)
                                                                            gcgGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGccc                   <  2:1324897/101‑1 (MQ=255)
                                                                            gcgGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGccc                   <  1:1581973/101‑1 (MQ=255)
                                                                            gcgGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGccc                   <  2:1944469/101‑1 (MQ=255)
                                                                             cgGCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCg                  <  1:2741518/101‑1 (MQ=255)
                                                                              ggCTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCgg                 <  1:4994937/101‑1 (MQ=255)
                                                                                cTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGTCCCGgcg               <  2:3074250/101‑1 (MQ=255)
                                                                                cTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGgcg               >  1:1095674/1‑101 (MQ=255)
                                                                                cTGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGgcg               >  2:899562/1‑101 (MQ=255)
                                                                                 tGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGgcgc              >  1:4654260/1‑101 (MQ=255)
                                                                                 tGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGgcgc              >  2:3771516/1‑101 (MQ=255)
                                                                                 tGGGGGTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGgcgc              >  2:3423070/1‑101 (MQ=255)
                                                                                  gggggTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGNGCGCAAgnnnnnnnnnn             >  2:1437953/1‑91 (MQ=255)
                                                                                   ggggTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGgcgcgc            >  2:3990155/1‑101 (MQ=255)
                                                                                    gggTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGcgcgcg           <  2:1862178/101‑1 (MQ=255)
                                                                                    gggTGGTCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGcgcgcg           <  2:3021374/101‑1 (MQ=255)
                                                                                       tggtCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGcc        <  1:4823270/101‑1 (MQ=255)
                                                                                       tggtCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGcc        <  1:1847548/101‑1 (MQ=255)
                                                                                         gtCGGCGGGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCgg      >  1:979954/1‑101 (MQ=255)
                                                                                             gcggGCTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCGGGCTg  <  1:1237315/101‑1 (MQ=255)
                                                                                                  |                                                                                               
TGACGCGGACCGGGGCGATCAGCGCGCTGGCGCGGCTTCTGTCGAACCCGGCGCTGCCGGTCAGCCTGCATGTGGCGCGGCTGGGGGTGGTCGGCGGGGTGCTTTCGAGCGTGATGAACAATGTCACCGCGCTGGCGCTTTTGATGCCCGTCGACATCGCCACGGCGCGCAAGGCCCGGCGCGCGGCCGGGCTG  >  NC_014034/2462352‑2462545

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG