Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,467,061 G→C 100% V29L (GTC→CTC)  RCAP_rcc02292 → signal transduction histidine kinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,467,0610G→C100.0% 761.6 204V29L (GTC→CTC) RCAP_rcc02292signal transduction histidine kinase
Reads supporting (aligned to +/- strand):  new base (102/102):  ref base (0/0):  total (102/102)

Only 100 of 206 total aligned reads displayed.

GGGCCGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGGTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAGGT  >  NC_014034/2466963‑2467158
                                                                                                  |                                                                                                 
gggCCGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTc                                                                                                 <  1:3019961/101‑1 (MQ=255)
 ggCCGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCa                                                                                                <  1:4144371/101‑1 (MQ=255)
   ccGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGc                                                                                              <  1:4547828/101‑1 (MQ=255)
    cGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCt                                                                                             >  2:4524964/1‑101 (MQ=255)
     gCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTa                                                                                            >  2:2790708/1‑101 (MQ=255)
      cAGGCGGCATTATTCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTAt                                                                                           <  1:3381364/101‑1 (MQ=255)
      cAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTAt                                                                                           <  2:2550933/101‑1 (MQ=255)
      cAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTAt                                                                                           <  1:1924073/101‑1 (MQ=255)
       aGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATg                                                                                          >  1:2108485/1‑101 (MQ=255)
         gcggcATGATCCGGTGGCGACGCGGCAGGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATgcg                                                                                        <  1:4673040/101‑1 (MQ=255)
          cggcATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATgcgc                                                                                       >  1:4120282/1‑101 (MQ=255)
            gcATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAg                                                                                     <  1:3232971/101‑1 (MQ=255)
             cATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGa                                                                                    >  2:2970311/1‑101 (MQ=255)
               ttaTCCGGTGGGGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGAcc                                                                                  <  1:4985491/99‑1 (MQ=255)
               tGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGAcc                                                                                  <  2:2134405/101‑1 (MQ=255)
                gATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACTGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGAccc                                                                                 <  2:3947118/101‑1 (MQ=255)
                gATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGAccc                                                                                 >  2:2797435/1‑101 (MQ=255)
                  tCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTg                                                                               <  2:2144006/101‑1 (MQ=255)
                  tCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTg                                                                               <  1:1881945/101‑1 (MQ=255)
                  tCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTg                                                                               >  1:3912992/1‑101 (MQ=255)
                    cGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGc                                                                             >  2:1287478/1‑101 (MQ=255)
                     ggTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGcc                                                                            <  2:4079310/101‑1 (MQ=255)
                      gTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGccc                                                                           >  2:3780689/1‑100 (MQ=255)
                      gTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCa                                                                           >  2:4671275/1‑101 (MQ=255)
                       tGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCAc                                                                          >  1:184527/1‑101 (MQ=255)
                        ggCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCAcc                                                                         <  1:4704427/101‑1 (MQ=255)
                          cGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGa                                                                       >  2:4960678/1‑101 (MQ=255)
                          cGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGa                                                                       >  1:1768496/1‑101 (MQ=255)
                          cGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGa                                                                       >  1:2927795/1‑101 (MQ=255)
                           gACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGaa                                                                      >  2:3716626/1‑101 (MQ=255)
                           gACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGaa                                                                      >  1:1714171/1‑101 (MQ=255)
                             cgcgGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACg                                                                    <  1:933469/101‑1 (MQ=255)
                             cgcgGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACg                                                                    <  1:3479986/101‑1 (MQ=255)
                                 gCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGgcc                                                                >  1:258162/1‑101 (MQ=255)
                                 gCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGgcc                                                                <  1:3423196/101‑1 (MQ=255)
                                   aCGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGgccgc                                                              <  2:2206950/101‑1 (MQ=255)
                                   aCGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATACGCAGACCCTGGCCACCGAACGGgccgc                                                              >  2:4877780/1‑101 (MQ=255)
                                     gggCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGccgccg                                                            <  1:4799411/101‑1 (MQ=255)
                                      ggCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGa                                                           >  1:2995014/1‑101 (MQ=255)
                                      ggCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGa                                                           <  1:4897762/101‑1 (MQ=255)
                                       gCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGaa                                                          <  1:3775576/101‑1 (MQ=255)
                                       gCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGaa                                                          <  2:4729311/101‑1 (MQ=255)
                                        gggTCGGGGGCTGCGGCTGACGGTGATCATGGGTGCGGTGCTGGCGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGaaa                                                         <  1:364577/100‑1 (MQ=255)
                                        cGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGaaa                                                         >  2:3586601/1‑101 (MQ=255)
                                        cGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGaaa                                                         <  2:289189/101‑1 (MQ=255)
                                         ggTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGaaaa                                                        >  1:1008077/1‑101 (MQ=255)
                                         ggTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGaaaa                                                        <  1:2311339/101‑1 (MQ=255)
                                           tCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAAcc                                                      >  2:1554104/1‑101 (MQ=255)
                                           tCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAAcc                                                      <  1:3850847/101‑1 (MQ=255)
                                            cTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCg                                                     >  2:3005233/1‑101 (MQ=255)
                                             tGGGGCTGCGGCTGACGGTGATCATGNNGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGt                                                    <  1:3591003/101‑1 (MQ=255)
                                             tGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGt                                                    >  1:1531737/1‑101 (MQ=255)
                                              ggggCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTc                                                   <  2:1858815/101‑1 (MQ=255)
                                               gggCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCt                                                  >  1:3681959/1‑101 (MQ=255)
                                               gggCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCt                                                  <  1:1945446/101‑1 (MQ=255)
                                                  cTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTgcg                                               <  1:2504083/101‑1 (MQ=255)
                                                  cTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTgcg                                               <  2:4145024/101‑1 (MQ=255)
                                                    gCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTgcgcg                                             >  2:1927162/1‑101 (MQ=255)
                                                    gCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTgcgcg                                             >  1:5038316/1‑101 (MQ=255)
                                                    gCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTgcgcg                                             <  2:4849861/101‑1 (MQ=255)
                                                     cGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTgcgcgc                                            <  1:2969905/101‑1 (MQ=255)
                                                     cGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTgcgcgc                                            >  1:1621687/1‑101 (MQ=255)
                                                      ggCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGcgcgcg                                           >  2:3425641/1‑101 (MQ=255)
                                                      ggCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGcgcgcg                                           >  2:3502882/1‑101 (MQ=255)
                                                       gCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCgcgg                                          <  2:1717722/101‑1 (MQ=255)
                                                       gCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCgcgg                                          >  2:603506/1‑101 (MQ=255)
                                                            cGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATc                                     >  2:3521093/1‑101 (MQ=255)
                                                             ggTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATcc                                    >  2:4587507/1‑101 (MQ=255)
                                                             ggTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATcc                                    >  1:656876/1‑101 (MQ=255)
                                                              gTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCt                                   <  2:726397/101‑1 (MQ=255)
                                                               tGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTg                                  >  1:2842683/1‑101 (MQ=255)
                                                               tGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTg                                  <  2:1693454/101‑1 (MQ=255)
                                                                gATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTgg                                 >  2:1148256/1‑101 (MQ=255)
                                                                  tCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGc                               <  2:3897511/101‑1 (MQ=255)
                                                                   cATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCg                              >  1:3486523/1‑101 (MQ=255)
                                                                   cATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCg                              >  1:4660138/1‑101 (MQ=255)
                                                                   cATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATACGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCg                              <  1:4878107/101‑1 (MQ=255)
                                                                    aTGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCga                             >  1:819901/1‑101 (MQ=255)
                                                                        gggCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACg                         >  2:202283/1‑101 (MQ=255)
                                                                           cGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTg                      <  1:4473370/101‑1 (MQ=255)
                                                                           cGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGAAGCTg                      <  1:1100944/101‑1 (MQ=255)
                                                                            ggTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGa                     >  1:1153306/1‑101 (MQ=255)
                                                                            ggTCCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGa                     <  2:3681681/101‑1 (MQ=255)
                                                                               gCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATgg                  >  2:3081526/1‑101 (MQ=255)
                                                                               gCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATgg                  >  2:2708874/1‑101 (MQ=255)
                                                                                 tGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCtcgacgcc                <  2:2857904/101‑8 (MQ=255)
                                                                                 tGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgcc                >  1:3206916/1‑101 (MQ=255)
                                                                                 ggACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgcc                <  1:1287599/100‑1 (MQ=255)
                                                                                    cgccgcTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCCGATGgccgcc             <  2:4329190/101‑1 (MQ=255)
                                                                                     gccgcTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGccgccg            <  2:2379536/101‑1 (MQ=255)
                                                                                      ccgcTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCcgccgc           >  2:525290/1‑101 (MQ=255)
                                                                                         cTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCcgccgcgcc        <  1:3972463/101‑1 (MQ=255)
                                                                                            gCCGTGCTCAGCTATGCGCAGACCCTGGCAACCGAACGGCCCGCCGAAAACCGGCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCa     <  2:860141/101‑1 (MQ=255)
                                                                                             ccGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTTGGCGAGACGCTGCTGGCCGCCGCGCCGCAg    >  2:4841716/1‑101 (MQ=255)
                                                                                             ccGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAg    >  2:3995989/1‑101 (MQ=255)
                                                                                              cGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAgg   >  1:1504759/1‑101 (MQ=255)
                                                                                              cGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAgg   >  1:3213224/1‑101 (MQ=255)
                                                                                               gTGCTCAGCTATGCGCAGCCCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAGGt  <  2:3209499/101‑1 (MQ=255)
                                                                                               gTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAGGt  >  1:4771929/1‑101 (MQ=255)
                                                                                               gTGCTCAGCTAAGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAGGt  >  1:2426658/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GGGCCGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGGTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAGGT  >  NC_014034/2466963‑2467158

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG