Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,481,028	C→G	100%	G333A (GGG→GCG)	RCAP_rcc02306 ←	LysM domain‑containing protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,481,028	0	C→G	100.0%	202.2	54	G333A (GGG→GCG)	RCAP_rcc02306	LysM domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (49/5):  ref base (0/0):  total (49/5)

TCTCGGCAACCGCGGCGGCGGCGGGCGGCGGCGGCGGGGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCCCATCAAGGCGCATCGCATGCGGCCCGGGCGTCTCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCA  >  NC_014034/2480933‑2481123                                                                                                |                                                                                                tctcGGCAACCGCGGCGGCGGCGGGCGGCGGCGGCGGGGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCa                                                                                            <  1:2950141/101‑1 (MQ=255)             cggcggcggcgggcggcggcggcgggGGGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCCCCCGGCCCGCGGCCCCGAGCGCATCAAGGcgcagcgca                                                                                >  2:4292499/1‑101 (MQ=255)                  gcggcgggcggcggcggcgggGCGGCGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCgg                                                                           <  1:4292816/101‑1 (MQ=255)                        ggcggcggcggcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCggg                                                                     >  2:3764589/1‑101 (MQ=255)                          cggcggcggcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCg                                                                   >  2:373659/1‑101 (MQ=255)                           ggcggcggcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGt                                                                  >  1:3275999/1‑101 (MQ=255)                              ggcggcgggGCGACGCCCCCGAAGCCCCGCTCCGCGCGGGGCAGCACCCGGCCCGCGGCATCCAGCGCATCAAGGCGCAGCGCACGCGGCCCGGGCGtcgc                                                               >  2:4354777/1‑99 (MQ=255)                                  gcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCcgcg                                                           >  2:4011579/1‑101 (MQ=255)                                     gggCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTTGCCCCGGGc                                                        >  2:3318854/1‑101 (MQ=255)                                       gCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGGCGCCGCGGGcgc                                                      >  1:911873/1‑99 (MQ=255)                                             cccTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGccg                                                >  2:4974251/1‑99 (MQ=255)                                             cccTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAg                                                >  1:4547746/1‑101 (MQ=255)                                               cTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCg                                              >  1:100696/1‑101 (MQ=255)                                                 cGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCggg                                            >  2:4560020/1‑99 (MQ=255)                                                 cGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCgcg                                            >  1:2425368/1‑99 (MQ=255)                                                 cGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTg                                            >  1:1957145/1‑101 (MQ=255)                                                  gAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGa                                           >  1:2470752/1‑101 (MQ=255)                                                  gAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGa                                           >  1:2016635/1‑101 (MQ=255)                                                  gAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGa                                           >  2:590136/1‑101 (MQ=255)                                                  gAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGa                                           >  1:4284056/1‑101 (MQ=255)                                                  gAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGa                                           <  2:4547426/101‑1 (MQ=255)                                                     gCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAAc                                        >  1:2290659/1‑101 (MQ=255)                                                      cccTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAAcc                                       >  1:1626336/1‑101 (MQ=255)                                                       ccTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAAccc                                      >  2:1766496/1‑100 (MQ=255)                                                        cTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAAccgc                                     >  1:4822242/1‑101 (MQ=255)                                                          gCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAAccgccg                                   >  2:2892094/1‑101 (MQ=255)                                                           cTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGt                                  >  2:3261446/1‑101 (MQ=255)                                                             cGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTcc                                >  1:1053627/1‑101 (MQ=255)                                                             cGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTcc                                >  2:2567965/1‑101 (MQ=255)                                                             cGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTcc                                >  1:2632233/1‑101 (MQ=255)                                                              ggCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTccc                               >  2:3768308/1‑101 (MQ=255)                                                                 gcgGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCcccgc                            >  2:4728919/1‑101 (MQ=255)                                                                  cgGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCcccgcc                           >  2:850816/1‑101 (MQ=255)                                                                  cgGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCcccgcc                           >  2:616629/1‑101 (MQ=255)                                                                   gggCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCcccgccc                          >  2:1257221/1‑101 (MQ=255)                                                                   gggCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCcccgccc                          >  2:3471255/1‑101 (MQ=255)                                                                   gggCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCcccgccc                          >  1:2489459/1‑101 (MQ=255)                                                                    ggCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCcccgcccg                         <  2:4821915/101‑1 (MQ=255)                                                                      ccAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCcgcccgcc                       >  2:4688022/1‑101 (MQ=255)                                                                      ccAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGAACGGCATGCGGCCCGGCCGTCGCCCCGGGCAGGCGCGCCCCGAAACCCCCGTGcccgccccgc                       >  2:1512982/1‑98 (MQ=255)                                                                        aGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTg                     >  2:4073104/1‑101 (MQ=255)                                                                         gcccccGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCCCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGc                    <  2:2489285/98‑1 (MQ=255)                                                                         gCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGc                    >  1:4799692/1‑101 (MQ=255)                                                                         gCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGc                    >  2:1193183/1‑101 (MQ=255)                                                                           aCCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGGCCCCGCCCGCCTGCAc                  >  2:39312/1‑101 (MQ=255)                                                                             ccGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCa                >  1:1710459/1‑101 (MQ=255)                                                                              cGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGGGAAACCGCCGTCCCCGCCCGCCTGCACCac               >  2:1733007/1‑100 (MQ=255)                                                                                gCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAAcc             >  2:2603560/1‑101 (MQ=255)                                                                                  ccGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAAcccc           <  1:4560341/101‑1 (MQ=255)                                                                                     cgacATCGAGCGCATCAGGGCGGAGCCGAGGCGGCCGGGGCCGCGCCGGGGGCAGCCGCAGCGTGAACCCGCCGTCCCCGCCCGCCTGCACCAACCCCAcc        <  1:3319116/98‑1 (MQ=255)                                                                                      ggCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCt       >  2:1518009/1‑101 (MQ=255)                                                                                       gCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTc      >  2:2677362/1‑101 (MQ=255)                                                                                       gCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTc      >  1:660324/1‑101 (MQ=255)                                                                                       gCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTc      >  1:1574588/1‑101 (MQ=255)                                                                                          tCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGc   >  1:3236288/1‑101 (MQ=255)                                                                                           cGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCa  >  2:4111720/1‑101 (MQ=255)                                                                                           cGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCa  >  1:3336864/1‑101 (MQ=255)                                                                                           cGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCa  >  2:5011929/1‑101 (MQ=255)                                                                                           cGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCa  >  2:911556/1‑101 (MQ=255)                                                                                           cGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCCCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCGTGCACCAACCCCACCTCGGcc  >  1:2078266/1‑100 (MQ=255)                                                                                                |                                                                                                TCTCGGCAACCGCGGCGGCGGCGGGCGGCGGCGGCGGGGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCCCATCAAGGCGCATCGCATGCGGCCCGGGCGTCTCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCA  >  NC_014034/2480933‑2481123 Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG