Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,016 C→G 3.0% intergenic (+64/‑178) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0160C→G 3.0% 5.0 ‑1.8 ‑0.7167intergenic (+64/‑178)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (70/92):  total (74/93)
Fisher's exact test strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.88e-01

Only 100 of 182 total aligned reads displayed.

CGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGG.CG..CTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC  >  NC_014034/2493917‑2494115
                                                                                                       |                                                                                                         
cgccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAcc                                                                                                           >  1:1958933/1‑101 (MQ=21)
 gccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCg                                                                                                          <  1:269230/101‑1 (MQ=21)
 gccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCg                                                                                                          <  1:4489113/101‑1 (MQ=21)
  ccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCgg                                                                                                         <  1:3951055/101‑1 (MQ=21)
  ccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCgg                                                                                                         <  2:4804522/101‑1 (MQ=21)
    ggggcgggcgCGCGGCACGCCAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAt                                                                                                       <  2:4291956/99‑1 (MQ=18)
    gcggcgGGCGCGCGGCACGACAGGCCGAGGAA.GGGGGGG.GGGCCGGCCCCCCGCCCCCAACGGGACCCCCCCCCGGCGATTTCCACCAAAATAAAAACGGa                                                                                                        >  1:902978/1‑101 (MQ=255)
     cggcggGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATg                                                                                                      <  1:610261/101‑1 (MQ=21)
       gcgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGcc                                                                                                    <  1:1338150/101‑1 (MQ=21)
        cgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCt                                                                                                   <  2:3568878/101‑1 (MQ=21)
         gggcgCGCGGCACGACAGGCCGAGGGA.GGGGGGGACG..GTGCCCCCCGTCCGCAAGCGGCCTCTCACCCGGTTTTTGGCAGCGAGAGGGAACCTGAttgcct                                                                                                  >  2:162605/1‑96 (MQ=9)
          ggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGGCTCGCCCCCGGCTAATTGCAGCAAGATTAAAGCGGGTGGCttt                                                                                                 >  2:578350/1‑101 (MQ=12)
          ggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                 <  2:3009561/101‑1 (MQ=21)
          ggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                 <  2:1945107/101‑1 (MQ=21)
            cgcgcgGCACGACAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                               <  2:2737233/101‑1 (MQ=21)
                cagCACGACAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                           <  2:5014979/99‑1 (MQ=18)
                  gCACGACAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGc                                                                                         >  2:4969158/1‑101 (MQ=21)
                    ccGACAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                       <  1:276658/100‑1 (MQ=18)
                    aCGGCAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                       <  1:2325626/101‑1 (MQ=18)
                    aCGACAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                       <  1:3164033/101‑1 (MQ=21)
                    aCGACAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                       <  1:1085005/101‑1 (MQ=21)
                    aCGACAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                       >  1:2294179/1‑101 (MQ=21)
                     cGACAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                      <  1:389726/101‑1 (MQ=21)
                        cAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                   >  2:2443809/1‑101 (MQ=21)
                        cAGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                   <  1:577467/101‑1 (MQ=21)
                         aGGCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                                  <  1:1911015/101‑1 (MQ=21)
                         aGGCCGAGTAAGGGGGGGG.CC..CTTCCCCCCCTCCGCAAGGGGGCCCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                                  <  2:3696304/101‑1 (MQ=21)
                          ggCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                 <  1:739234/101‑1 (MQ=21)
                           gCCGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                                <  1:3600293/101‑1 (MQ=18)
                            ccGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                               <  1:168796/101‑1 (MQ=21)
                             cGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                              <  2:2942548/101‑1 (MQ=21)
                             cGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                              <  2:4482938/101‑1 (MQ=21)
                             cGAGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                              <  2:1492206/101‑1 (MQ=18)
                               aGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTTCCCGAAATATccca                                                                            >  1:2444096/1‑100 (MQ=18)
                               aGTAAGGGGGGGG.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                            <  1:4580936/101‑1 (MQ=18)
                                gtaagggggggg.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                           >  2:50858/6‑101 (MQ=255)
                                 taagggggggg.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCCAGATGAAACCGGGTGCCTTTCATCTTGCCCGAAATATTCCggg                                                                          >  2:1893391/5‑101 (MQ=18)
                                 taagggggggg.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                          >  1:3848389/5‑101 (MQ=255)
                                 taagggggggg.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCCAAAAATCCCgcg                                                                          >  2:3525894/5‑99 (MQ=18)
                                 taaggaggggg.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCCAGATGAAACCGGGTGCCTTTCATCTTGCCCGAAATATCCCggg                                                                          >  1:333415/7‑101 (MQ=17)
                                  aagggggggg.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                         <  1:2046447/98‑1 (MQ=255)
                                   agggggggg.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCCGCAAGATGAAACCCGATGCCTTTCATCTTGCCCGAACTATCCCggggg                                                                        >  2:1216163/3‑101 (MQ=18)
                                   agggggggg.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                        >  1:4942366/3‑101 (MQ=255)
                                    gggggggg.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                       >  2:2513676/2‑101 (MQ=255)
                                       ggggg.CG..CTGCCCCCCGTGCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATCAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                    <  1:5026068/101‑1 (MQ=18)
                                        gggg.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATTCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                   <  2:1670615/101‑1 (MQ=255)
                                         ggg.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCCGGCTATTTGCAGCAAGATGAAACCGGAAGCATTTCCTCTTTCCCGAAAAATCCCggggggggcat                                                                  >  1:1162576/1‑96 (MQ=17)
                                          gg.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                <  2:4312072/101‑1 (MQ=255)
                                          gg.CG..CTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                <  1:2366847/101‑1 (MQ=255)
                                           g.cg..cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGCAATATCCCGGGGGGTGAA.Tgg                                                               <  1:2876840/101‑1 (MQ=255)
                                           g.cg..cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tgg                                                               >  2:4718543/1‑101 (MQ=255)
                                             cg..cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                              <  1:4217268/101‑1 (MQ=255)
                                              g..cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                           >  1:944133/1‑101 (MQ=255)
                                                  tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                        >  1:2942809/1‑100 (MQ=255)
                                                   ccccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                       <  2:295632/100‑3 (MQ=255)
                                                    cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                     <  1:1753572/101‑5 (MQ=255)
                                                    cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                     <  1:3490442/101‑5 (MQ=255)
                                                    cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                     <  1:2236576/101‑5 (MQ=255)
                                                    cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                     <  2:3848104/101‑5 (MQ=255)
                                                    cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                     <  2:1958789/101‑5 (MQ=255)
                                                    cccccCGTCCGCAAGCGGCCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                      <  2:3354399/101‑4 (MQ=21)
                                                    cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                      <  1:779015/101‑4 (MQ=255)
                                                     ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                    >  2:1580692/1‑96 (MQ=255)
                                                          gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGCAATATCCCGGGGGGCGAA.TGGC..G.aagccagagg                                                >  2:4054418/2‑92 (MQ=18)
                                                          gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                <  2:4234184/100‑10 (MQ=255)
                                                                 aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCag                                          <  2:2294015/101‑2 (MQ=18)
                                                                 aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCag                                          <  2:1010559/101‑2 (MQ=18)
                                                                  gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagc                                         <  2:2245006/101‑3 (MQ=18)
                                                                   cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGATATATCCCGGGGGGCGAA.TGGC..GAAGCCAGAGGGGGGCagcg                                        >  2:5013465/1‑98 (MQ=17)
                                                                   cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCaggg                                        >  2:2108998/1‑98 (MQ=18)
                                                                   cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcg                                        >  2:234936/1‑98 (MQ=18)
                                                                    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGTGGGGGGAA.TGGC..GAAGCCAGAGGGGGGCagcgc                                       >  1:4296424/1‑97 (MQ=17)
                                                                    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcgc                                       >  1:4922635/1‑97 (MQ=17)
                                                                        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cc.ccc                                >  2:3924517/1‑101 (MQ=17)
                                                                              cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGccc                           <  2:1778835/101‑1 (MQ=17)
                                                                                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CC.CCCTGCCCcg                         >  1:414303/1‑101 (MQ=2)
                                                                                ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCcg                         <  1:1580824/101‑1 (MQ=17)
                                                                                ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCcg                         >  1:4557965/1‑101 (MQ=17)
                                                                                ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCcg                         <  1:4273383/101‑1 (MQ=17)
                                                                                 gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgc                        >  1:1106325/1‑100 (MQ=2)
                                                                                 gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAGCCAGAGGGGGGCAGCGG.CC.CCCTGCCCcgg                        >  2:3780334/1‑101 (MQ=17)
                                                                                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgcc                       <  2:2567939/101‑3 (MQ=2)
                                                                                  atatTTGCAGGAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.GGGC..GAAGCCAGAGGGGGGCCGGGC.CC.CCCTGCCCcgcc                       >  1:1654281/1‑99 (MQ=17)
                                                                                  atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCcggc                       >  1:1947906/1‑101 (MQ=17)
                                                                                   tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCcggcg                      >  1:2956470/1‑101 (MQ=17)
                                                                                   tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCcggcg                      >  2:4221845/1‑101 (MQ=18)
                                                                                    atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCcggcgg                     >  2:715411/1‑101 (MQ=18)
                                                                                     tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGt                    <  2:1530092/101‑1 (MQ=17)
                                                                                      ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTc                   <  1:1445728/101‑1 (MQ=17)
                                                                                      ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTc                   >  1:3754824/1‑101 (MQ=18)
                                                                                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTcc                  >  1:3037526/1‑101 (MQ=17)
                                                                                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTcc                  >  2:1798383/1‑101 (MQ=17)
                                                                                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGG.GAGCG.CCGCCCTGCCCCGGCGGTcc                  >  2:1902392/1‑101 (MQ=18)
                                                                                       tGCACNNAGATGCAACCGGATTCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTcc                  <  1:1976333/101‑1 (MQ=14)
                                                                                            caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCCGAGGGGGGC.AGCGCCC.CCCTCCCCCAGCCGTCCCTggc             >  2:887342/1‑101 (MQ=9)
                                                                                              aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCCCGCGGTCCGTggcgg           >  2:3144664/1‑101 (MQ=17)
                                                                                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCtt     <  2:2912032/101‑1 (MQ=17)
                                                                                                     aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg    <  2:3904594/101‑1 (MQ=17)
                                                                                                     aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg    >  2:3661741/1‑101 (MQ=18)
                                                                                                     aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg    <  1:4969488/101‑1 (MQ=17)
                                                                                                       |                                                                                                         
CGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGG.CG..CTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC  >  NC_014034/2493917‑2494115

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG