Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,018 G→C 3.0% intergenic (+66/‑176) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0180G→C 3.0% 5.0 ‑1.7 ‑0.8164intergenic (+66/‑176)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (71/88):  total (75/89)
Fisher's exact test strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.77e-01

Only 100 of 181 total aligned reads displayed.

CCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGG.CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.C.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGG  >  NC_014034/2493919‑2494117
                                                                                                     |                                                                                                          
ccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCgg                                                                                                            <  1:3951055/101‑1 (MQ=21)
ccgcGGCGGGCGCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCgg                                                                                                            <  2:4804522/101‑1 (MQ=21)
  ggggcgggcgCGCGGCACGCCAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAt                                                                                                          <  2:4291956/99‑1 (MQ=18)
    ggcgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTTCAGCAAGATGAAACCGGATGc                                                                                                        >  1:5001242/1‑101 (MQ=18)
    ggcgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGc                                                                                                        <  1:4543829/101‑1 (MQ=21)
     gcgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCCGCAAGATGAAACCGGATGcc                                                                                                       >  1:2245161/1‑101 (MQ=18)
     gcgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGcc                                                                                                       <  1:1338150/101‑1 (MQ=21)
       gggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                     <  1:4471302/101‑1 (MQ=21)
       gggcgCGCGGCACGACAGGCCGAGGGA.GGGGGGGACGGTGCCCCCCGTCCGCAAGCGGCCTCTCACCCGGTTTTTGGCAGCGAGAGGGAACCTGAttgcct                                                                                                     >  2:162605/1‑96 (MQ=9)
        ggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGGCTCGCCCCCGGCTAATTGCAGCAAGATTAAAGCGGGTGGCttt                                                                                                    >  2:578350/1‑101 (MQ=12)
        ggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                    <  2:3009561/101‑1 (MQ=21)
        ggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                    <  2:1945107/101‑1 (MQ=21)
         gcgcgcGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                   <  2:151780/101‑1 (MQ=21)
         gcgcgcGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                   <  1:2397304/101‑1 (MQ=21)
          cgcgcgGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                  <  1:937133/101‑1 (MQ=21)
              cagCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                              <  2:5014979/99‑1 (MQ=18)
                gCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGc                                                                                            >  2:4969158/1‑101 (MQ=21)
                  ccGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                          <  1:276658/100‑1 (MQ=18)
                  aCGGCAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                          <  1:2325626/101‑1 (MQ=18)
                  aCGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                          <  1:4140015/101‑1 (MQ=21)
                  aCGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                          <  1:3164033/101‑1 (MQ=21)
                  aCGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                          <  1:739906/101‑1 (MQ=21)
                  aCGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                          <  1:1085005/101‑1 (MQ=21)
                   cggcAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                         <  2:4052052/98‑1 (MQ=18)
                   cGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                         <  1:389726/101‑1 (MQ=21)
                      cAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                      <  1:577467/101‑1 (MQ=21)
                       aGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                                     <  1:1911015/101‑1 (MQ=21)
                       aGGCCGAGTAAGGGGGGGG.CCCTTCCCCCCCTCCGCAAGGGGGCCCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                                     <  2:3696304/101‑1 (MQ=21)
                         gCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                                   <  1:3600293/101‑1 (MQ=18)
                         gCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                                   <  2:1716759/101‑1 (MQ=21)
                          ccGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                                  <  1:168796/101‑1 (MQ=21)
                           cGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                 <  2:3591959/101‑1 (MQ=21)
                           cGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                 <  2:4482938/101‑1 (MQ=21)
                           cGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                 <  2:2103101/101‑1 (MQ=18)
                            taGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                                <  2:2443922/100‑1 (MQ=18)
                             aGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                               <  1:4580936/101‑1 (MQ=18)
                              gtaagggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                              >  2:50858/6‑101 (MQ=255)
                               taagggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCCAGATGAAACCGGGTGCCTTTCATCTTGCCCGAAATATTCCggg                                                                             >  2:1893391/5‑101 (MQ=18)
                               taagggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                             >  1:3848389/5‑101 (MQ=255)
                               taagggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCCAAAAATCCCgcg                                                                             >  2:3525894/5‑99 (MQ=18)
                               taaggaggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCCAGATGAAACCGGGTGCCTTTCATCTTGCCCGAAATATCCCggg                                                                             >  1:333415/7‑101 (MQ=17)
                                 agggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCCGCAAGATGAAACCCGATGCCTTTCATCTTGCCCGAACTATCCCggggg                                                                           >  2:1216163/3‑101 (MQ=18)
                                     ggggg.CGCTGCCCCCCGTGCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATCAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                       <  1:5026068/101‑1 (MQ=18)
                                       ggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                    <  1:231201/101‑1 (MQ=255)
                                       ggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCCGGCTATTTGCAGCAAGATGAAACCGGAAGCATTTCCTCTTTCCCGAAAAATCCCggggggggcat                                                                     >  1:1162576/1‑96 (MQ=17)
                                         g.cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGCAATATCCCGGGGGGTGAA.Tgg                                                                  <  1:2876840/101‑1 (MQ=255)
                                         g.cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tgg                                                                  >  2:4718543/1‑101 (MQ=255)
                                           cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                 <  1:4217268/101‑1 (MQ=255)
                                              tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                           >  1:2942809/1‑100 (MQ=255)
                                               gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                          >  1:4935839/1‑99 (MQ=255)
                                               ccccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                          <  2:295632/100‑3 (MQ=255)
                                                cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                        <  2:3848104/101‑5 (MQ=255)
                                                cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                        <  1:3490442/101‑5 (MQ=255)
                                                cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                        <  1:1753572/101‑5 (MQ=255)
                                                cccccCGTCCGCAAGCGGCCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                         <  2:3354399/101‑4 (MQ=21)
                                                 ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                       >  2:1580692/1‑96 (MQ=255)
                                                   cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAAAATCCCGGGGGGTGAA.TGGC..G.aagccag                                                      >  1:2081755/5‑95 (MQ=21)
                                                      gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGCAATATCCCGGGGGGCGAA.TGGC..G.aagccagagg                                                   >  2:4054418/2‑92 (MQ=18)
                                                      gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                   <  2:4234184/100‑10 (MQ=255)
                                                             aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCag                                             >  2:2861193/1‑100 (MQ=18)
                                                             aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCag                                             <  2:2294015/101‑2 (MQ=18)
                                                              gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagc                                            <  2:2245006/101‑3 (MQ=18)
                                                               cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGATATATCCCGGGGGGCGAA.TGGC..GAAGCCAGAGGGGGGCagcg                                           >  2:5013465/1‑98 (MQ=17)
                                                               cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcg                                           >  2:234936/1‑98 (MQ=18)
                                                                ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGTGGGGGGAA.TGGC..GAAGCCAGAGGGGGGCagcgc                                          >  1:4296424/1‑97 (MQ=17)
                                                                ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcgc                                          >  1:4959002/1‑97 (MQ=17)
                                                                 gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.c.c                                      >  1:1954739/1‑101 (MQ=17)
                                                                   cTCCCCCCGGGATATTTGCAGCAAGTTGAAACCGGATGCCCTTCATCTTGCCCGAAATATCCCGGGGGGTGAG.TCGC..GAAGCCACAGGGGGGC.AGCG.c.c.cc                                   >  2:1792817/1‑101 (MQ=17)
                                                                          cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCCTCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGccc                             >  1:1996975/1‑101 (MQ=17)
                                                                            ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCcg                           <  1:4273383/101‑1 (MQ=17)
                                                                            ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCcg                           <  1:1580824/101‑1 (MQ=17)
                                                                             gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.C.C.CCCTGCCCcgc                          >  1:1106325/1‑100 (MQ=2)
                                                                             gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAGCCAGAGGGGGGCAGCGG.C.C.CCCTGCCCcgg                          >  2:3780334/1‑101 (MQ=17)
                                                                              atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.C.C.CCCTGCCCcgcc                         <  2:2567939/101‑3 (MQ=2)
                                                                              atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.C.C.CCCTGCCCcgcc                         >  1:831521/1‑99 (MQ=2)
                                                                              atatTTGCAGGAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.GGGC..GAAGCCAGAGGGGGGCCGGGC.C.C.CCCTGCCCcgcc                         >  1:1654281/1‑99 (MQ=17)
                                                                              atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCcgcg                         >  1:2509077/1‑99 (MQ=17)
                                                                              atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCcggc                         >  1:1947906/1‑101 (MQ=17)
                                                                               tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCcggcg                        >  2:4221845/1‑101 (MQ=18)
                                                                               tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCcggcg                        >  1:2956470/1‑101 (MQ=17)
                                                                                atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCcggcgg                       >  2:715411/1‑101 (MQ=18)
                                                                                 tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGt                      <  2:1530092/101‑1 (MQ=17)
                                                                                  ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTc                     >  1:3754824/1‑101 (MQ=18)
                                                                                  ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTc                     <  1:1445728/101‑1 (MQ=17)
                                                                                   tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTcc                    >  1:3037526/1‑101 (MQ=17)
                                                                                   tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTcc                    >  2:1798383/1‑101 (MQ=17)
                                                                                   tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGG.GAGCG.C.CGCCCTGCCCCGGCGGTcc                    >  2:1902392/1‑101 (MQ=18)
                                                                                    gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCg                   <  1:67356/101‑1 (MQ=17)
                                                                                     cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCcggcggggcgt                  >  2:4463382/1‑96 (MQ=17)
                                                                                        caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCCGAGGGGGGC.AGCGCC.C.CCCTCCCCCAGCCGTCCCTggc               >  2:887342/1‑101 (MQ=9)
                                                                                        caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTggc               <  2:256841/101‑1 (MQ=17)
                                                                                          aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCCGCGGTCCGTggcgg             >  2:3144664/1‑101 (MQ=17)
                                                                                           gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTggcggc            >  1:991197/1‑101 (MQ=17)
                                                                                               aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCt        >  2:29184/1‑101 (MQ=17)
                                                                                                 aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg      <  2:3904594/101‑1 (MQ=17)
                                                                                                 aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg      >  2:3661741/1‑101 (MQ=18)
                                                                                                  ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa     >  2:2153091/1‑101 (MQ=18)
                                                                                                   cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc    >  1:4841375/1‑101 (MQ=17)
                                                                                                    ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCC.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg   <  2:3017367/101‑1 (MQ=17)
                                                                                                     gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGAGAA.TGGC..GAAGCCAGAGGGGGGC.AGCA.CAC.CCCTGCCCCGGCGGTCCGTGGCGGGGCCCTGAcgg  >  1:836473/1‑101 (MQ=12)
                                                                                                     |                                                                                                          
CCGCGGCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGG.CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.C.C.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGG  >  NC_014034/2493919‑2494117

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG