Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,523,952 T→G 100% I573S (ATC→AGC)  ppiD → peptidyl‑prolyl cis‑trans isomerase D

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,523,9520T→G100.0% 859.4 224I573S (ATC→AGC) ppiDpeptidyl‑prolyl cis‑trans isomerase D
Reads supporting (aligned to +/- strand):  new base (122/102):  ref base (0/0):  total (122/102)

Only 100 of 229 total aligned reads displayed.

CGACAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGATCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCAA  >  NC_014034/2523854‑2524049
                                                                                                  |                                                                                                 
cGACAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGcc                                                                                                 <  2:3437096/101‑1 (MQ=255)
 gACAAGGCCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCa                                                                                                >  1:524863/1‑101 (MQ=255)
   cAAGGTCTTTGTCGTCACCGTGCGCGGGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGa                                                                                              <  1:180860/101‑1 (MQ=255)
   cAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGa                                                                                              >  1:2956918/1‑101 (MQ=255)
    aaGGTCTTTGTCGTCCCCCTGCGCGCGGGGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGAt                                                                                             <  2:2407890/101‑1 (MQ=255)
    aaGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTCGACACCCGGCTGAGCCAGAt                                                                                             <  2:2398703/101‑1 (MQ=255)
    aaGGTCTTTGTCGCCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGAt                                                                                             <  2:1590850/101‑1 (MQ=255)
    aaGGTCTTCGCCGTAGCCCTGCGCCCGGTGAAACCGGCCGATCCGGCCGATCCCGGGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGAt                                                                                             <  1:3724571/101‑1 (MQ=255)
      ggTCTTTGTCTTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGc                                                                                           <  1:3881879/101‑1 (MQ=255)
      ggTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGc                                                                                           >  1:4154002/1‑101 (MQ=255)
       gTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCt                                                                                          >  2:3693591/1‑101 (MQ=255)
       gTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCt                                                                                          >  1:3502758/1‑101 (MQ=255)
         cTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCg                                                                                        >  1:2336398/1‑101 (MQ=255)
          tttGTCGTCCCCGGCCGCGCGGTGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCgg                                                                                       <  2:2705240/101‑1 (MQ=255)
           ttGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGgc                                                                                      <  2:4023983/101‑1 (MQ=255)
           ttGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGgc                                                                                      <  2:1436476/101‑1 (MQ=255)
              tcgtcACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGcc                                                                                   >  2:4795181/1‑101 (MQ=255)
              tcatccccGTGCGCGCGGTGAAACCGCCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGcc                                                                                   <  2:2058800/95‑1 (MQ=255)
               cgtcACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCg                                                                                  >  1:98957/1‑101 (MQ=255)
               cgtcACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCg                                                                                  >  1:791214/1‑101 (MQ=255)
               cgtcACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCg                                                                                  >  1:2876500/1‑101 (MQ=255)
               cgtcACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCg                                                                                  >  2:2319177/1‑101 (MQ=255)
               cgtcACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCg                                                                                  >  2:625333/1‑101 (MQ=255)
                  cACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATc                                                                               >  1:261520/1‑101 (MQ=255)
                   cctgTGCGCGCGTTGAAACCGGCCGATCCGTCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCt                                                                              <  2:4094369/98‑1 (MQ=255)
                   cccGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCt                                                                              <  1:1368660/100‑1 (MQ=255)
                   aCCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCt                                                                              >  2:3531894/1‑101 (MQ=255)
                   aCCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCt                                                                              >  1:4073469/1‑101 (MQ=255)
                       tGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTggcg                                                                          >  1:40633/1‑101 (MQ=255)
                            gcgGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCtt                                                                     <  2:2731803/101‑1 (MQ=255)
                            gcgGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCtt                                                                     >  1:1064653/1‑101 (MQ=255)
                             cgGGGAGACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACTCCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTc                                                                    <  1:819726/101‑1 (MQ=255)
                              ggTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCt                                                                   <  2:326204/101‑1 (MQ=255)
                              ggTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCt                                                                   >  2:4221232/1‑101 (MQ=255)
                              ggTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCt                                                                   >  1:4283516/1‑101 (MQ=255)
                              ggTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCt                                                                   >  2:4142115/1‑101 (MQ=255)
                               gTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTa                                                                  <  1:2516987/101‑1 (MQ=255)
                               gTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTa                                                                  <  1:3260518/101‑1 (MQ=255)
                                tGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTAc                                                                 <  1:3815150/101‑1 (MQ=255)
                                tGAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTAc                                                                 <  1:1549949/101‑1 (MQ=255)
                                tGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTAc                                                                 >  1:3621355/1‑101 (MQ=255)
                                ggAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTAc                                                                 <  2:3502488/100‑1 (MQ=255)
                                ggAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTAc                                                                 <  1:2975580/100‑1 (MQ=255)
                                 gAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACg                                                                <  1:3204530/101‑1 (MQ=255)
                                 gAACCCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACg                                                                <  2:1723487/101‑1 (MQ=255)
                                 gAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACg                                                                <  2:1386410/101‑1 (MQ=255)
                                     ccggccgatccggccgatCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCg                                                            <  1:2492099/101‑1 (MQ=255)
                                      cggccgatccggccgatcCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGt                                                           <  1:1296876/101‑1 (MQ=255)
                                       ggccgatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTg                                                          >  2:2524451/1‑101 (MQ=255)
                                          cgatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGGGCCGATCTGGCGGCCTTCTACGCCCGTGCcg                                                       >  1:5006592/1‑101 (MQ=255)
                                          cgatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCcg                                                       >  1:3074613/1‑101 (MQ=255)
                                          cgatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCcg                                                       >  1:4898650/1‑101 (MQ=255)
                                          cgatccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCcg                                                       >  1:2383010/1‑101 (MQ=255)
                                            atccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTCCGCCCGTGctccc                                                     >  2:1574803/1‑97 (MQ=255)
                                              ccggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCa                                                   >  2:861922/1‑101 (MQ=255)
                                                ggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCagg                                                 >  1:556990/1‑101 (MQ=255)
                                                ggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCagg                                                 <  1:4704763/101‑1 (MQ=255)
                                                ggccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCagg                                                 >  1:2418462/1‑101 (MQ=255)
                                                 gccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggc                                                >  1:1399479/1‑101 (MQ=255)
                                                 gccgatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggc                                                >  1:2193947/1‑101 (MQ=255)
                                                    gatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATGTGGCGGCCTTCTACGCCCGTGCCGCGCaggccga                                             <  2:2902992/101‑1 (MQ=255)
                                                    gatccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccga                                             <  1:269955/101‑1 (MQ=255)
                                                     atccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgag                                            >  1:964786/1‑101 (MQ=255)
                                                     atccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgag                                            >  2:395492/1‑101 (MQ=255)
                                                     atccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgag                                            <  2:1784748/101‑1 (MQ=255)
                                                     atccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgag                                            <  2:3171758/101‑1 (MQ=255)
                                                     atccCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgag                                            >  2:3032228/1‑101 (MQ=255)
                                                     atcatgAGCTGGTGCAGCAGCGCAATACATTTGATACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgag                                            <  2:4872575/96‑1 (MQ=255)
                                                       cccGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCaggccgaggc                                          <  2:3681111/101‑1 (MQ=255)
                                                           aGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCggg                                      <  1:2442337/101‑1 (MQ=255)
                                                             cTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGAt                                    >  1:1679630/1‑101 (MQ=255)
                                                              tGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATc                                   <  1:3693869/101‑1 (MQ=255)
                                                               ggTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCa                                  >  2:2946907/1‑101 (MQ=255)
                                                                gTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCAc                                 >  1:2488977/1‑101 (MQ=255)
                                                                gTGCAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCAc                                 <  2:2354197/101‑1 (MQ=255)
                                                                   cAGCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCt                              >  2:2200036/1‑101 (MQ=255)
                                                                     gCTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCg                            <  2:1429366/101‑1 (MQ=255)
                                                                      cTGCGCAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGa                           <  2:4549554/101‑1 (MQ=255)
                                                                        gcgcAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACa                         <  2:1060192/101‑1 (MQ=255)
                                                                        gcgcAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACa                         >  2:1926264/1‑101 (MQ=255)
                                                                         cgcAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGCTCACCCTCGACAg                        >  1:4342214/1‑101 (MQ=255)
                                                                          gcAAGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAgc                       >  1:4588012/1‑101 (MQ=255)
                                                                            aaGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAgcgc                     <  2:440324/101‑1 (MQ=255)
                                                                             aGACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGcgcg                    <  1:3532164/101‑1 (MQ=255)
                                                                              gACCTTTGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCga                   >  2:1289558/1‑101 (MQ=255)
                                                                                  tttGACACCCGGCTGAGCCAGATGCTCGGCGCGGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCgatgat               <  1:5027334/101‑1 (MQ=255)
                                                                                  tttGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCgatgat               >  2:202260/1‑101 (MQ=255)
                                                                                   ttGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATc              <  2:4879560/101‑1 (MQ=255)
                                                                                   ttGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATc              >  1:3297856/1‑101 (MQ=255)
                                                                                   ttGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATc              <  1:1535666/101‑1 (MQ=255)
                                                                                    tGACACCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCg             >  1:4870689/1‑101 (MQ=255)
                                                                                        ccccGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGc         <  2:261486/100‑1 (MQ=255)
                                                                                        acCCGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGc         >  1:1982559/1‑101 (MQ=255)
                                                                                          ccGGCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCg       <  2:2694357/101‑1 (MQ=255)
                                                                                            ggCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTg     <  1:1595539/101‑1 (MQ=255)
                                                                                             gCTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGTAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGc    >  2:528441/1‑101 (MQ=255)
                                                                                              cTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCa   >  1:4120342/1‑101 (MQ=255)
                                                                                              cTGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCa   <  1:1862996/101‑1 (MQ=255)
                                                                                               tGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATGACCCTCGACAGCGCGATGGTCGCTGCCGTGCaa  >  1:4359966/1‑101 (MQ=255)
                                                                                               tGAGCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCaa  <  2:3914544/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CGACAAGGTCTTTGTCGTCACCGTGCGCGCGGTGAAACCGGCCGATCCGGCCGATCCCGAGCTGGTGCAGCTGCGCAAGACCTTTGACACCCGGCTGATCCAGATGCTCGGCGCCGATCTGGCGGCCTTCTACGCCCGTGCCGCGCAGGCCGAGGCCGGGATCACCCTCGACAGCGCGATGATCGCTGCCGTGCAA  >  NC_014034/2523854‑2524049

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG