Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,559,923 G→C 100% intergenic (‑199/+71) ispZ ← / ← ftsY intracellular septation protein A/cell division protein FtsY

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,559,9230G→C100.0% 455.5 127intergenic (‑199/+71)ispZ/ftsYintracellular septation protein A/cell division protein FtsY
Reads supporting (aligned to +/- strand):  new base (106/20):  ref base (0/1):  total (106/21)

Only 100 of 181 total aligned reads displayed.

TCGCCCGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGGGGGGGG.CAGCGCCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCG.ATCCCCACCAGCGCG  >  NC_014034/2559825‑2560021
                                                                                                      |                                                                                                     
tcgCCCGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAgcgg                                                                                                     >  1:1391572/1‑101 (MQ=255)
     cGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.c                                                                                               <  2:2096042/101‑1 (MQ=255)
     cGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.c                                                                                               <  2:3022931/101‑1 (MQ=255)
     cGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.c                                                                                               <  2:1148357/101‑1 (MQ=255)
       gTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAg                                                                                             <  1:2163321/101‑1 (MQ=255)
       gTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGCCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAg                                                                                             <  2:4900248/101‑1 (MQ=255)
        tGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAgc                                                                                            <  2:4819870/101‑1 (MQ=255)
        tGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAgc                                                                                            <  2:587656/101‑1 (MQ=255)
        tGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAgc                                                                                            <  2:2662511/101‑1 (MQ=255)
         gCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAgcg                                                                                           >  1:4211354/1‑101 (MQ=255)
           ggTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCGcc                                                                                         >  1:3094601/1‑101 (MQ=255)
           ggTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGGTGCGCG.TCAGCGC.AAGCGGGGGG.CAGAGcc                                                                                         >  2:2806276/1‑101 (MQ=255)
            gTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCGccc                                                                                        >  1:1540734/1‑101 (MQ=255)
             tttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCGcccc                                                                                       <  1:4803890/101‑1 (MQ=255)
              ttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCGccccc                                                                                      <  1:736028/101‑1 (MQ=255)
              ttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCGccccc                                                                                      >  1:1501799/1‑101 (MQ=255)
               tCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCGcccccc                                                                                     <  2:1501669/101‑1 (MQ=255)
                ccTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCGccccccg                                                                                    >  1:191933/1‑100 (MQ=255)
                ccTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCGccccccg                                                                                    >  1:1416933/1‑100 (MQ=255)
                 cTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCGccccccga                                                                                   >  2:4677606/1‑99 (MQ=255)
                   gACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCGccccccgaag                                                                                 >  1:5004939/1‑97 (MQ=255)
                    aCAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAgc                                                                              >  1:70303/1‑101 (MQ=255)
                    aCAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAgc                                                                              >  2:3605131/1‑101 (MQ=255)
                      aGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAgcgc                                                                            >  2:3076008/1‑101 (MQ=255)
                      aGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAgcgc                                                                            >  1:100287/1‑101 (MQ=255)
                      aGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAgcgc                                                                            >  1:3054635/1‑101 (MQ=255)
                         aTTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.C.GCCGCCCCCC.GAAACGCCTg                                                                         >  1:5014599/1‑101 (MQ=255)
                             tgtCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGgc                                                                     >  2:769773/1‑101 (MQ=255)
                              gtCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCGGCGC.AAGCGGGGGG.AAGCG.CCCCCC.GAAGCGCCTGAGgcg                                                                    >  1:825823/1‑101 (MQ=255)
                               tCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGgcgc                                                                   >  2:1999556/1‑101 (MQ=255)
                                ccTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAACG.CCCCCC.GAAGCGCCGGAGGcgcg                                                                  >  2:2412604/1‑101 (MQ=255)
                                   ccgcgCCGC.GCGGGCCCCTTTTTCCCTGGGGCAAAC.CGGGGGGGGGTGGCGCG..CCGCGG.AAGGGGGGGG.GCGCCCCCCCCC.GAAGCGCCTGAGGCgcggcg                                                               <  2:1918654/100‑1 (MQ=255)
                                    cgcgCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCGCCCCCCC..AAGCGCCTGCGGCGcggcgg                                                              >  1:1794232/1‑101 (MQ=255)
                                      cgccgcAGCGGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTGGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGc                                                            <  2:681952/101‑1 (MQ=39)
                                       gccgcAGCAGGCCCGCTTTT.CCTGGGGCGAAC.CGCGGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGC.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCt                                                           <  2:3876016/101‑1 (MQ=255)
                                           cagcagGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTgcc                                                       >  2:529727/1‑101 (MQ=255)
                                            agcagGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTgccc                                                      >  1:3231409/1‑101 (MQ=255)
                                             gcagGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTgcccg                                                     >  2:1552469/1‑101 (MQ=255)
                                             gcagGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGGTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAATCGCCTGATGCGCGGCGGGCTTgcccc                                                     >  2:1649783/1‑100 (MQ=38)
                                              cgggCCCGCTTTT.CTCGGGGAGAAA.CG.CGGGGGGGGGCGCGCGCCGCGCGAAGGGGGGGC.CCCCCCCCCCCG.GAAGCGCCTGAGGCGCGGAGGGCTTgccc                                                      <  1:5030806/99‑1 (MQ=255)
                                               agGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTgcccgcc                                                   >  1:281988/1‑101 (MQ=255)
                                               agGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTgcccgcc                                                   >  1:2346856/1‑101 (MQ=255)
                                               agGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTgcccgcc                                                   >  2:3364748/1‑101 (MQ=255)
                                                gcccgtttttt.CTTTGGGCAAAC.CGCGGGGGGGTGGCGGG.CCGGCG..AAGGGGGGGG.CCGCCCCCCCCG.GAAGCGCCTGAGGCGCGGCGGGCTTgcccgccc                                                  <  2:4601188/94‑1 (MQ=16)
                                                 gccgtcTTTT.CTTGGGGCGAAC.CGCGGGGGGGTGGCGCG.AAAGCGC.AGGGGGGGGG.CGGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCa                                                 <  1:1624269/96‑1 (MQ=21)
                                                  cccGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCaa                                                >  1:2203840/1‑101 (MQ=255)
                                                   ccGTTTTT.CCTTGGGCGAAC.CGCGGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAAc                                               <  1:1475666/101‑1 (MQ=25)
                                                     gtgttt.CCTTGGGCGAAC.CGCCGGGGGTTTGCGCG.TCAGCGA.AGGCGGGGGG.CACCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGa                                             <  2:1463443/98‑1 (MQ=25)
                                                     gCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGa                                             >  1:1289550/1‑101 (MQ=255)
                                                      cTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGGCTGCCCGCCCCACGaa                                            >  2:3515088/1‑101 (MQ=255)
                                                      cTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCCCCCCACCaa                                            >  2:1824379/1‑101 (MQ=37)
                                                        ttt.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACCaaag                                          >  1:2680281/1‑99 (MQ=37)
                                                         tt.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCGCGCCCCACGCAggg                                         >  1:1086905/1‑100 (MQ=255)
                                                         tt.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGgc                                         >  1:3623211/1‑101 (MQ=255)
                                                          tccggggggAGAAC.CGCGGGGGGGTGGGGGG.CCAGCGA.AGGGGGGGGG.CCGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGgc                                         <  2:4684183/95‑1 (MQ=16)
                                                          t.CCTGGGGCAAAACCGCCGGGGGGTGGCGCG.TGAGCGC.AGGGGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGgc                                         <  2:562188/101‑1 (MQ=21)
                                                            ccTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGgcgc                                       >  1:3756687/1‑101 (MQ=255)
                                                            ccTGGGGGGAAA.CGCGGGGGGGTTGCGCG.TCAGCGC.AGGGGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGgcgc                                       <  2:794336/101‑1 (MQ=25)
                                                             cTTGGGCGAAA.CGCCGGGGGGTTGTGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCCTGCCCGCCCAACCAAAGCGCa                                      >  1:1748331/1‑101 (MQ=255)
                                                             cTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCCACGAAGGCGCa                                      >  2:2839216/1‑101 (MQ=255)
                                                             cTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCa                                      >  1:2853944/1‑101 (MQ=255)
                                                              ttGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCaa                                     >  2:164674/1‑101 (MQ=255)
                                                               tGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAac                                    >  1:4013873/1‑101 (MQ=255)
                                                                gggCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAacc                                   >  1:2776926/1‑101 (MQ=255)
                                                                 ggCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCCACGAAGGCGCAacca                                  >  1:3292603/1‑101 (MQ=255)
                                                                   ggAAG.CGCGGGGGGGTGGCGCG.GCGGCGG.AAGGGGGGGC.CGGC.ACCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAaccacc                                <  2:3210001/100‑1 (MQ=18)
                                                                     aaa.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAaccaccct                              >  2:110556/1‑99 (MQ=255)
                                                                      aa.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTc                             >  2:2102239/1‑101 (MQ=255)
                                                                       a.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCa                            >  1:365492/1‑101 (MQ=255)
                                                                         ggggggggtgTGGCGCG.CCGGCG..AAGGGGGGGG.GCCCCCCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCATCGAAGGCGCAACCACCTTCAg                           <  1:2872338/97‑1 (MQ=18)
                                                                         cGCCGGGGGGTGGCGCG.TCAGGGC.ACGCGGCGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAg                           <  2:4196032/101‑1 (MQ=21)
                                                                             ggggggTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCCACGAAGGCGCAACCACCCTCCGTCgg                       >  1:3428207/1‑101 (MQ=25)
                                                                             ggggggTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCgg                       >  2:4582747/1‑101 (MQ=255)
                                                                                gggTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGGTTTCCCGCCCCACGAAGGGGCAACCCCCTTTCGTCGGCtt                    >  1:2631561/1‑101 (MQ=255)
                                                                                gggTTGCGCG.CCAGCGC.AAGCGGGGGG.CAGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCtt                    <  2:4122084/101‑1 (MQ=255)
                                                                                 gtgggcgcG.CGAGCGC.GGGGGGGGGC.CCGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTc                   <  2:365439/97‑1 (MQ=21)
                                                                                  gtggcgcG.TGAGCGC.AGGCGGGGGG.CGGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCg                  <  2:1518017/98‑1 (MQ=25)
                                                                                  gtggcgcG.CCAGCGC.AAGGGGGGGG.CAGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCg                  <  2:337211/98‑1 (MQ=255)
                                                                                  gTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTcc                  >  2:1037159/1‑100 (MQ=255)
                                                                                  gTTGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCg                  >  1:4870274/1‑101 (MQ=255)
                                                                                   ttGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCG.a                >  1:4274395/1‑101 (MQ=255)
                                                                                    tGCGCG.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCG.At               >  2:4395993/1‑101 (MQ=255)
                                                                                        gg.TCAGCGC.AAGGGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCG.ATcccc           <  2:4850662/100‑1 (MQ=255)
                                                                                        cgggccgggc.AAGGCGGGGG.CCCCCCCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCG.ATcc             <  2:3995331/93‑1 (MQ=25)
                                                                                        cg.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCG.ATcccc           >  2:3952195/1‑101 (MQ=255)
                                                                                         g.TCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCG.ATCCcca          >  2:236061/1‑101 (MQ=255)
                                                                                         cgtgcccgc.AGGCGGGGGG.CCGCG.CCCCGG.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCG.ATcccc           <  2:1391446/95‑1 (MQ=21)
                                                                                         cggcAGCGC..AGGGGGGGG.CCCCCCCCCCCG.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCG.ATcccc           <  2:4491082/98‑1 (MQ=25)
                                                                                           tCAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCCTTCCCGCCCCACCAAAGCGCGAGCACCCTTAGCCGCCTTGGAATccccc          >  1:4235571/1‑100 (MQ=12)
                                                                                           ggcgcgcggggggggggg.CCCCCCCCCCCCGGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCG.ATccc            <  2:4416104/91‑1 (MQ=21)
                                                                                           gccagggaaaGGGGGGGG.CGGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCG.ATCCcca          <  2:2346692/93‑1 (MQ=25)
                                                                                            cAGCGC.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCG.ATCCccacc        >  1:3922865/1‑101 (MQ=255)
                                                                                             cgcgc.GAGGGGGGGGCCCCCCCCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGCCGCAACCACCTTCAGTCGGCTTCG.ATCCccac         <  2:4233122/100‑1 (MQ=25)
                                                                                              cggcggggggggggg.GCGCCCCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCG.ATCCccacc        <  1:774751/94‑1 (MQ=25)
                                                                                              cgcgtgggggggggg.CACCCCCCCCAG.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCG.ATCCccacc        <  2:228677/94‑1 (MQ=25)
                                                                                               agcccaGGGGGGGG.CCCCCCCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCG.ATCCccacca       <  1:539393/96‑1 (MQ=37)
                                                                                                gc.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCG.ATCCCCACCAgcg    >  2:3071031/1‑101 (MQ=255)
                                                                                                gc.AAGCGGGGGG.CACCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAAACACCTTCAGTCCGNTTCG.ATTCCCACCAgcg    >  2:2139689/1‑101 (MQ=25)
                                                                                                 c.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCG.ATCCCCACCAgcgc   >  2:3089879/1‑101 (MQ=255)
                                                                                                 c.AAGCGGGGGG.CAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCG.ATCCCCACCAgcgc   >  2:2861921/1‑101 (MQ=255)
                                                                                                      |                                                                                                     
TCGCCCGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTT.CCTTGGGCGAAA.CGCCGGGGGGTTGCGCG.TCAGCGC.AAGGGGGGGG.CAGCGCCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCG.ATCCCCACCAGCGCG  >  NC_014034/2559825‑2560021

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG