Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,566,607 T→A 2.4% intergenic (+79/+115) purD → / ← sdaA phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,566,6070T→A 2.4% 5.0 0.0 ‑0.0205intergenic (+79/+115)purD/sdaAphosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  new base (2/3):  ref base (100/100):  total (102/103)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.98e-01

Only 100 of 231 total aligned reads displayed.

CGGCTGGCGCGCGCTCTGAGACGAAAC.GGGGG.CGCTGCCCCC.CTCCTG..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGG  >  NC_014034/2566510‑2566707
                                                                                                      |                                                                                                     
cGGCTGGCGCGCGCTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCt                                                                                                       <  1:535576/101‑1 (MQ=21)
 ggCTGGCGCGCGCTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtt                                                                                                      <  2:337048/101‑1 (MQ=21)
 ggCTGGCGCGCGCTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtt                                                                                                      <  1:2939631/101‑1 (MQ=21)
 ggCTGGCGCGCGCTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtt                                                                                                      >  2:4666595/1‑101 (MQ=21)
   cTGGCGCGCGCTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtttt                                                                                                    <  2:174030/101‑1 (MQ=21)
     ggCGCGCGCTCTGAAACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTcc                                                                                                  <  1:3651329/101‑1 (MQ=21)
       cgcgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                                                                                <  1:226240/101‑1 (MQ=21)
        gcgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                                                                                               <  1:3285597/101‑1 (MQ=21)
         cgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                                                                                              <  2:192013/101‑1 (MQ=21)
         cgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                                                                                              <  2:2870611/101‑1 (MQ=21)
         cgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                                                                                              <  2:3302022/101‑1 (MQ=21)
          gcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCa                                                                                             <  2:426189/101‑1 (MQ=21)
           cgcTCTGAGACGAAAC.GGGGGTCGCTTCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCAt                                                                                            <  1:1616734/101‑1 (MQ=25)
            gcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                                                                                           <  1:3191357/101‑1 (MQ=21)
            gcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                                                                                           <  1:2154159/101‑1 (MQ=21)
            gcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                                                                                           <  1:2565977/101‑1 (MQ=21)
             ctctGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTTATCt                                                                                          >  1:2119601/1‑101 (MQ=21)
             ctctGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCt                                                                                          >  2:4678419/1‑101 (MQ=21)
             ctctGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCAGGCAAGATAAAGAAGGTCTTTTCCTTTCATCt                                                                                          <  2:1093383/101‑1 (MQ=21)
              tctGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt                                                                                         >  1:3091542/1‑101 (MQ=21)
              tctGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTCTCCTTTCATCtt                                                                                         <  1:2728105/101‑1 (MQ=21)
               ctGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                                                                                        <  2:4898629/101‑1 (MQ=21)
                tGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGc                                                                                       <  1:4249700/101‑1 (MQ=21)
                tGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGc                                                                                       <  2:1073410/101‑1 (MQ=21)
                  agaCGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGccc                                                                                     <  1:1748694/101‑1 (MQ=21)
                   gaCGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCg                                                                                    <  2:3407080/101‑1 (MQ=21)
                    aCGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.a                                                                                  <  2:539174/101‑1 (MQ=21)
                    aCGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.a                                                                                  <  2:4727329/101‑1 (MQ=21)
                     cGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.aa                                                                                 >  1:3357414/1‑101 (MQ=21)
                     cGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGACATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.aa                                                                                 >  2:613597/1‑101 (MQ=21)
                      gaagcgggggg.CGCAGCCCCC.CTCTTGGCCATCGGCCAAGTCAGCCACAGGGATATATCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.aaa                                                                                <  2:4183595/95‑1 (MQ=255)
                      gAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.aaa                                                                                >  2:229319/1‑101 (MQ=21)
                       aaacgggggg.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAat                                                                               >  1:3827214/6‑101 (MQ=21)
                       aaacgggggg.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAat                                                                               <  1:3944356/96‑1 (MQ=21)
                       aaacgggggg.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGAAATTCCGGCCAAAAAAAGAAAGGACTTTTCTTTTCTTCTTCCCCG.aaaa                                                                               >  2:4359411/6‑100 (MQ=2)
                       aaacgggggg.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCCGGATATTTCGGGCAAGATAAAGAAGGGCTTTTCCTTTCATCTTGCCCC.AAat                                                                               >  1:2033105/6‑101 (MQ=21)
                        ggcaggggg.CGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGC.AAATATCCCgg                                                                        >  1:4977195/5‑101 (MQ=0)
                        aacgggggg.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTATTTTCCTTTCATCTTGCCCG.AAata                                                                              >  1:557794/5‑101 (MQ=21)
                         acgggggg.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCTCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAatat                                                                             <  2:3198269/98‑1 (MQ=25)
                         acgggggg.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAatat                                                                             <  1:2975659/98‑1 (MQ=25)
                          cgggggg.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATc                                                                            >  2:3038455/3‑101 (MQ=25)
                          cgggggg.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATc                                                                            <  2:237318/99‑1 (MQ=25)
                            ggggg.CGCTGCCCCCTCTTGGC..CTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGC.AAATATCCCgggggg                                                                    <  2:1478495/101‑2 (MQ=2)
                             gggg.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCg                                                                         <  2:2456950/101‑1 (MQ=25)
                             gggg.CCCTGCCCCC.TTTTGGC.CTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGC.AAATATCCCgggggga                                                                   <  2:359618/101‑3 (MQ=2)
                               gg.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCggg                                                                       >  2:2674843/1‑101 (MQ=25)
                                g.cgcTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCgggg                                                                      >  1:1121439/1‑101 (MQ=37)
                                      gCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGGCCG.AAATATCCCGGGGGAGcc                                                                 >  2:4973558/1‑101 (MQ=25)
                                      gCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGcc                                                                 <  2:4036376/101‑1 (MQ=37)
                                           ccctcttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCt                                                             <  1:2645069/91‑1 (MQ=255)
                                            cctcctggccTTCGCCCAATTCCCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCtt                                                            <  1:3930720/92‑1 (MQ=37)
                                               cttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTgcg                                                         >  1:3232992/7‑101 (MQ=255)
                                               cttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGCAGCCGGCTTccg                                                         >  2:2502701/7‑101 (MQ=37)
                                                 tggccTTCGGCCAATTCCCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTgcggg                                                       <  2:4504973/97‑1 (MQ=255)
                                                 tggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCCTgcggg                                                       >  2:240400/5‑101 (MQ=255)
                                                 tggccTTCGGCCAATTCACCCCCCGGGATAATTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AGATATCCCGGGGGAGCCCGCTTgcggg                                                       >  2:1612481/5‑101 (MQ=255)
                                                     cTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGcgggcggg                                                   >  1:3400121/1‑101 (MQ=255)
                                                     cTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGcgggcggg                                                   >  2:4518154/1‑101 (MQ=255)
                                                     cTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGcgggcggg                                                   <  1:4678742/101‑1 (MQ=255)
                                                       tCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCggggg                                                 >  1:2669553/1‑101 (MQ=255)
                                                        cGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGc                                                <  1:4162774/101‑1 (MQ=255)
                                                         ggCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCa                                               >  2:1382929/1‑101 (MQ=255)
                                                          ggcAAATCCCCCCCCGGGGTTTTTTCGGGAAAAAAAAAAAGGGTTTTTTCTTTCCTTTTTCCCGAAAATTCCCCGGGGGAGCCCGCTTGCGGGCGGGGGCa                                               <  1:1020567/99‑1 (MQ=255)
                                                              aTTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcc                                          >  1:1842656/1‑101 (MQ=255)
                                                                tccccccccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcccc                                        <  1:967887/98‑1 (MQ=255)
                                                                   ccccccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGa                                     <  2:1654836/101‑1 (MQ=255)
                                                                         gggATATTTCGGTCAATATAAAGAAGGTCTTTTCCTTTCTTCTTGCCCA.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCATCGCCCCCGAAGCGcc                               <  1:1987380/101‑1 (MQ=255)
                                                                         gggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATTTTGCCCG.AAATATCCCGGGGAAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGcc                               >  1:857155/1‑101 (MQ=255)
                                                                         gggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGcc                               >  1:3596420/1‑101 (MQ=255)
                                                                          ggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTTCGGGCGGGGGCAGGGCCCCCCAAGGGccc                              >  2:3462992/1‑101 (MQ=38)
                                                                           gATATTTGGGGCAAGATAAGGAAGGTTTTTTCTTTTCATCTTGCCGG.AAATACCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCg                             <  2:22593/101‑1 (MQ=255)
                                                                           gATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCg                             <  2:2749651/101‑1 (MQ=255)
                                                                            atatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCgc                            >  2:733843/1‑100 (MQ=255)
                                                                            atatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGa                            >  1:4393800/1‑101 (MQ=255)
                                                                            atatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGa                            >  2:3544099/1‑101 (MQ=255)
                                                                            atatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCCCCCGa                            >  2:1511156/1‑101 (MQ=255)
                                                                             tatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAc                           >  1:149204/1‑101 (MQ=255)
                                                                             tatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAc                           >  2:4480908/1‑101 (MQ=255)
                                                                              atTTCGTGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcg                          <  1:812310/101‑1 (MQ=255)
                                                                                   gggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcgg                     >  1:698429/1‑101 (MQ=255)
                                                                                   gggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcgg                     >  2:3133509/1‑101 (MQ=255)
                                                                                   gggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcgg                     >  1:466027/1‑101 (MQ=255)
                                                                                    ggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggc                    <  1:3030695/101‑1 (MQ=255)
                                                                                     gCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCCTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggcg                   >  1:4711090/1‑101 (MQ=255)
                                                                                      cAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGcggcgg                  >  1:1903281/1‑101 (MQ=255)
                                                                                      cAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGcggcgg                  >  1:2312313/1‑101 (MQ=255)
                                                                                       aaGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCggg                 >  2:88232/1‑101 (MQ=255)
                                                                                       aaGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCggg                 >  2:4406318/1‑101 (MQ=255)
                                                                                        aGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGc                >  1:650738/1‑101 (MQ=255)
                                                                                        aGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGc                >  2:3967174/1‑101 (MQ=255)
                                                                                         gATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCt               >  2:2084809/1‑101 (MQ=255)
                                                                                         gATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCt               >  2:2167219/1‑101 (MQ=255)
                                                                                          aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt              >  2:3760075/1‑101 (MQ=255)
                                                                                          aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt              >  2:47594/1‑101 (MQ=255)
                                                                                          aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt              >  2:484452/1‑101 (MQ=255)
                                                                                            aaaGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc            >  2:3912354/1‑101 (MQ=255)
                                                                                             aagaagGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcc           >  1:2834749/1‑101 (MQ=255)
                                                                                             aagaagGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcc           >  1:2454976/1‑101 (MQ=255)
                                                                                                     cTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAag   >  2:3847062/1‑101 (MQ=255)
                                                                                                      ttttCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCAGACGCGCGGCGGGCTTGCCCGCCCAagg  >  2:2041820/1‑101 (MQ=255)
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CGGCTGGCGCGCGCTCTGAGACGAAAC.GGGGG.CGCTGCCCCC.CTCCTG..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGG  >  NC_014034/2566510‑2566707

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG