Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,566,613 T→C 2.4% intergenic (+85/+109) purD → / ← sdaA phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,566,6130T→C 2.4% 3.2 ‑0.4 ‑0.0207intergenic (+85/+109)purD/sdaAphosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  new base (2/3):  ref base (112/90):  total (114/93)
Fisher's exact test strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.54e-01

Only 100 of 231 total aligned reads displayed.

GCGCGCGCTCTGAGACGAAAC.GGGGG.CGCTGCCCCC.CTCCTG..CTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCG  >  NC_014034/2566516‑2566713
                                                                                                        |                                                                                                      
gcgcgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCt                                                                                                        <  2:2861298/101‑1 (MQ=21)
gcgcgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GGCTTTTCCt                                                                                                        >  1:4505293/1‑101 (MQ=21)
 cgcgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCtt                                                                                                       <  1:2093234/101‑1 (MQ=21)
  gcgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCttt                                                                                                      <  1:3285597/101‑1 (MQ=21)
  gcgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCttt                                                                                                      <  2:4016807/101‑1 (MQ=21)
  gcgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCttt                                                                                                      <  1:1700501/101‑1 (MQ=21)
  gcgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCttt                                                                                                      <  1:4426888/101‑1 (MQ=21)
   cgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTc                                                                                                     <  2:406436/101‑1 (MQ=21)
   cgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTc                                                                                                     <  2:3302022/101‑1 (MQ=21)
   cgcgcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTc                                                                                                     <  2:1918172/101‑1 (MQ=21)
     cgcTCTGAGACGAAAC.GGGGGTCGCTTCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCAt                                                                                                   <  1:1616734/101‑1 (MQ=25)
      gcTCTGAGACGAACCGGGGGG.CGCTGCCCCC.TTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATc                                                                                                  <  1:2652893/101‑1 (MQ=21)
      gcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATc                                                                                                  <  2:2891000/101‑1 (MQ=21)
      gcTCTGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATc                                                                                                  <  1:3191357/101‑1 (MQ=21)
       ctctGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.t                                                                                                >  2:2387314/1‑101 (MQ=21)
       ctctGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.t                                                                                                <  2:3411039/101‑1 (MQ=21)
       ctctGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.AAATTTCGGGGCAGAAAAAAAAG.GGCTTTTTCttttcttt                                                                                                 >  1:899201/1‑96 (MQ=9)
        tctGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.tt                                                                                               >  1:3091542/1‑101 (MQ=21)
         ctGAGGCGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCTCCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTg                                                                                              <  1:2566418/101‑1 (MQ=21)
         ctGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTg                                                                                              <  2:4898629/101‑1 (MQ=21)
         ctGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTATCCTTTCATC.TTg                                                                                              <  2:1974418/101‑1 (MQ=21)
          tGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGc                                                                                             >  2:3223909/1‑101 (MQ=21)
          tGAGACGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.AAATCTCGGGCAAGATAAAGAAG.GTCGTTTCCTTTCATC.TTGc                                                                                             >  1:4849067/1‑101 (MQ=21)
           gagaCGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGcc                                                                                            >  1:4110983/1‑101 (MQ=21)
           gagaCGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCCGG.AGATTTTGGGGCAGATAAAAAAG.GGGTTTTTCTTTTATT.ttttc                                                                                            >  1:3572030/1‑98 (MQ=2)
             gaCGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCg                                                                                          <  1:1894315/101‑1 (MQ=21)
             gaCGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCg                                                                                          <  1:514012/101‑1 (MQ=21)
              aCGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.a                                                                                        <  2:539174/101‑1 (MQ=21)
              aCGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.a                                                                                        <  2:4727329/101‑1 (MQ=21)
               cGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.aa                                                                                       >  1:3357414/1‑101 (MQ=21)
               cGAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ACATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.aa                                                                                       >  2:613597/1‑101 (MQ=21)
                gAAACGGGGGG.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.aaa                                                                                      >  2:229319/1‑101 (MQ=21)
                 aaacgggggg.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAat                                                                                     <  2:107585/96‑1 (MQ=21)
                 aaacgggggg.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.AAATTCCGGCCAAAAAAAGAAAG.GACTTTTCTTTTCTTC.TTCCCCG.aaaa                                                                                     >  2:4359411/6‑100 (MQ=2)
                  aacgggggg.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTATTTTCCTTTCATC.TTGCCCG.AAata                                                                                    >  1:557794/5‑101 (MQ=21)
                   acgggggg.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAatat                                                                                   <  1:2975659/98‑1 (MQ=25)
                    cgggggg.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGTAAATTTGGGGC.AGATAAAAAAGAGCTTTTTCTTTTCTTT.TTGCCCC.aaaatc                                                                                   >  1:226992/3‑98 (MQ=0)
                    cgggggg.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATc                                                                                  <  2:1023372/99‑1 (MQ=25)
                    cgggggg.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATc                                                                                  <  2:237318/99‑1 (MQ=25)
                    cgggggg.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTACTTTCATC.TTGCCCG.AAATATc                                                                                  >  2:1100723/3‑101 (MQ=21)
                      ggggg.CGCTGCCCCCTCTTGGC..CTTTGGCCAATTCA.CCCCTGGG.ATATTTCGGGCAAGAT...GAA..ATCATGCCCCTTCATC.TTGCCGC.AAATATCCCgggggg                                                                          <  2:1478495/101‑2 (MQ=2)
                      ggggg.CGCTGCCCCCTCTTGGC..CTTTGGCCAATTCA.CCCCTGGG.ATATTTCGGGCAAGAT...GAA..ATCATGCCCCTTCATC.TTGCCGC.AAATATCCCgggggg                                                                          <  2:4231871/101‑2 (MQ=0)
                      ggggg.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATccc                                                                                <  1:2137791/101‑1 (MQ=25)
                      ggggg.CGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATccc                                                                                <  2:2453882/101‑1 (MQ=25)
                            cgcTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCggggg                                                                           <  2:4869814/101‑1 (MQ=37)
                                      cctcctggccTTCGCCCAATTCCCCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCtt                                                                  <  1:3930720/92‑1 (MQ=37)
                                         cttggccTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTgcg                                                               >  1:3232992/7‑101 (MQ=255)
                                         cttggccTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTgcg                                                               >  2:2497216/7‑101 (MQ=255)
                                           tggccTTCGGCCAATTCACCCCCCGGG.ATAATTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AGATATCCCGGGGGAGCCCGCTTgcggg                                                             >  2:1612481/5‑101 (MQ=255)
                                            ggccTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTTCCCG.AAATATCCCGGGGGAGCCCGCTTgcgggc                                                            >  1:2724557/4‑101 (MQ=255)
                                              ccTTCGGCCAATTCACCCCCCGGG.ATAATTTGGGCAAGATAAAAAAA.GGGTTTTTCTTTTATCTTTTCCCC.AAAATCCCCGGGGGGGCCCCCTGgcggggg                                                           >  1:4235308/2‑99 (MQ=12)
                                               cTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGcgggcggg                                                         <  1:4678742/101‑1 (MQ=255)
                                               cTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGcgggcggg                                                         >  2:4518154/1‑101 (MQ=255)
                                               cTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGcgggcggg                                                         >  1:3400121/1‑101 (MQ=255)
                                                tgcGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCgggg                                                        <  2:37821/99‑1 (MQ=255)
                                                  cGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGc                                                      >  1:2183399/1‑101 (MQ=255)
                                                   ggCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCa                                                     >  1:2006828/1‑101 (MQ=255)
                                                    ggcAAATCCCCCCCCGGG.GTTTTTTCGGGAAAAAAAAAAAG.GGTTTTTTCTTTCCTT.TTTCCCGAAAATTCCCCGGGGGAGCCCGCTTGCGGGCGGGGGCa                                                     <  1:1020567/99‑1 (MQ=255)
                                                        aTTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GGCTTTTTCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcc                                                >  2:2525011/1‑101 (MQ=255)
                                                          tCACCCCCCGGC.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCAGC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcccc                                              <  2:3964271/101‑1 (MQ=255)
                                                             ccccccGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGa                                           <  2:1654836/101‑1 (MQ=255)
                                                                   ggg.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATT.TTGCCCG.AAATATCCCGGGGAAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGcc                                     >  1:857155/1‑101 (MQ=255)
                                                                   ggg.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGcc                                     >  1:2764816/1‑101 (MQ=255)
                                                                    gg.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGccc                                    >  2:3162466/1‑101 (MQ=255)
                                                                       atatTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCgc                                  >  2:733843/1‑100 (MQ=255)
                                                                       atatTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGa                                  >  1:2907800/1‑101 (MQ=255)
                                                                       atatTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGa                                  >  2:3544099/1‑101 (MQ=255)
                                                                       atatTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCCCCCGa                                  >  2:1511156/1‑101 (MQ=255)
                                                                        tatTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAc                                 >  1:149204/1‑101 (MQ=255)
                                                                        tatTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAc                                 >  2:2717608/1‑101 (MQ=255)
                                                                         atTTCGTGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcg                                <  1:812310/101‑1 (MQ=255)
                                                                         atTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcg                                >  2:3639854/1‑101 (MQ=255)
                                                                           ttCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcg                              <  1:195066/101‑1 (MQ=255)
                                                                           ttCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcg                              >  1:3613787/1‑101 (MQ=255)
                                                                             cGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcgcg                            >  1:2528990/1‑101 (MQ=255)
                                                                              gggCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcgg                           >  1:698429/1‑101 (MQ=255)
                                                                               ggCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggc                          <  1:3030695/101‑1 (MQ=255)
                                                                                  aaGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCggg                       >  2:88232/1‑101 (MQ=255)
                                                                                  aaGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCggg                       >  2:4406318/1‑101 (MQ=255)
                                                                                   aGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGc                      >  1:650738/1‑101 (MQ=255)
                                                                                    gATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCt                     >  2:2084809/1‑101 (MQ=255)
                                                                                    gATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCt                     >  2:2167219/1‑101 (MQ=255)
                                                                                     aTAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt                    >  2:47594/1‑101 (MQ=255)
                                                                                      tAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATACCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTg                   <  2:3496729/101‑1 (MQ=255)
                                                                                       aaaGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc                  >  2:4333584/1‑101 (MQ=255)
                                                                                       aaaGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc                  >  1:2442162/1‑101 (MQ=255)
                                                                                        aagaag.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcc                 >  2:2240128/1‑101 (MQ=255)
                                                                                        aagaag.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcc                 >  2:4710155/1‑101 (MQ=255)
                                                                                            ag.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgcc             <  2:3057205/101‑1 (MQ=255)
                                                                                                 cTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGGAGCGCCCCCGAAGCGCCCCACGCCCCGCGGGGTTTCCCCCCCAag         >  2:4505076/1‑101 (MQ=37)
                                                                                                 cTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAag         >  2:3847062/1‑101 (MQ=255)
                                                                                                  ttttCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagg        >  2:4493705/1‑101 (MQ=255)
                                                                                                   tttCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagga       >  1:4260663/1‑101 (MQ=255)
                                                                                                    ttCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggag      >  1:1832277/1‑101 (MQ=255)
                                                                                                    ttCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggag      >  2:1395306/1‑101 (MQ=255)
                                                                                                     tCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggagg     >  1:3976060/1‑101 (MQ=255)
                                                                                                     tCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggagg     <  1:4710478/101‑1 (MQ=255)
                                                                                                      ccTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAggg    >  1:1137312/1‑101 (MQ=255)
                                                                                                        tttCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgcg  >  1:3808134/1‑101 (MQ=255)
                                                                                                        tttCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCCCCCAAGGAGGgcg  >  1:3831648/1‑101 (MQ=255)
                                                                                                        |                                                                                                      
GCGCGCGCTCTGAGACGAAAC.GGGGG.CGCTGCCCCC.CTCCTG..CTTCGGCCAATTCACCCCCCGGG.ATATTTCGGGCAAGATAAAGAAG.GTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCG  >  NC_014034/2566516‑2566713

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG