Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 257,090 C→G 100% intergenic (+24/‑175) secA → / → RCAP_rcc00221 preprotein translocase subunit SecA/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034257,0900C→G100.0% 685.6 187intergenic (+24/‑175)secA/RCAP_rcc00221preprotein translocase subunit SecA/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (91/95):  ref base (1/0):  total (92/95)

Only 100 of 206 total aligned reads displayed.

GAGCCCTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GCCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCG  >  NC_014034/256992‑257186
                                                                                                   |                                                                                                 
gAGCCCTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCg                                                                                                 >  2:3262826/1‑101 (MQ=255)
         cgcAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGt                                                                                       <  2:3186669/101‑1 (MQ=255)
          gcAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTg                                                                                      <  2:1867988/101‑1 (MQ=255)
            aaTGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.cgggggga                                                                                    >  1:3189996/1‑97 (MQ=255)
             aTGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGGGGGGC.GGGGGGAAc                                                                                   >  2:4011190/1‑101 (MQ=255)
             aTGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGGGGGCC.gggggggac                                                                                   >  2:4815757/1‑96 (MQ=255)
             aTGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGGGGGCC.gggggggac                                                                                   >  1:4145550/1‑96 (MQ=255)
              tGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAAcc                                                                                  <  1:1353234/101‑1 (MQ=255)
               gACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAAccc                                                                                 <  2:2371144/101‑1 (MQ=255)
                  ccctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGGC.GGGGGGGACCCggg                                                                              >  1:2723145/1‑101 (MQ=255)
                  ccctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCCGGGGGGGAACCCgg                                                                               >  1:3732990/1‑101 (MQ=255)
                   cctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGAAACCGGgc                                                                             >  2:2155703/1‑101 (MQ=255)
                   cctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCCGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGgc                                                                             <  1:2859560/101‑1 (MQ=255)
                    ctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGgcc                                                                            <  1:1170826/101‑1 (MQ=255)
                    ctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGGC.GGGGGGGACCCCGgcc                                                                            >  1:1033075/1‑101 (MQ=255)
                     tgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTGC.GGGGGGGAGGCGGgccc                                                                           >  1:2380922/1‑100 (MQ=255)
                      gcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGGGCG.GGGGGGAACCCGGgcgc                                                                           >  2:2219632/1‑99 (MQ=255)
                      gcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGGAACCGGgccgc                                                                          >  1:572672/1‑101 (MQ=255)
                       cccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGgccgcc                                                                         <  1:1964265/101‑1 (MQ=255)
                         cctggGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGt                                                                       <  1:3148103/101‑1 (MQ=255)
                          ctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGGACCCCGGgccccggt                                                                      >  1:4622465/1‑99 (MQ=255)
                            tcggtcagggCGAGAAGTTCAAGCCATGCCTCGGCCGTCTGGCTTTAAACATAGGCGCTGAACTATGGC.GTCGGTCC.GGGGTGAACCCGGGCCGCCGtttt                                                                    <  2:4977680/94‑1 (MQ=255)
                            gcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGtttt                                                                    <  2:4175153/101‑1 (MQ=255)
                              ggCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTc                                                                  <  2:1052935/101‑1 (MQ=255)
                              ggCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGGACCCCGGGCGCCGgttttt                                                                  >  2:1825768/1‑100 (MQ=255)
                              ggCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.CGGGGGAAACCCGGGCGCCGGTTTTc                                                                  >  2:352546/1‑101 (MQ=255)
                               gCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCa                                                                 <  1:1383237/101‑1 (MQ=255)
                               gCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGGACCCGGGCCGCCGgtttttc                                                                 >  1:4524651/1‑99 (MQ=255)
                                cTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCAt                                                                <  1:4228012/101‑1 (MQ=255)
                                 tCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGGACCCCGGCCCCCGgtttttctt                                                               >  1:2198736/1‑97 (MQ=255)
                                   gggCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGAAACCCGGCCGCCGGTTTTCCtttt                                                             >  1:2831394/1‑99 (MQ=255)
                                     gCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATcc                                                           <  1:1925300/101‑1 (MQ=255)
                                     gCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATcc                                                           <  1:919962/101‑1 (MQ=255)
                                      cGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCt                                                          <  2:2564787/101‑1 (MQ=255)
                                      cGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGGACCCGGGCCGCCGTTTTTTCTTATCCt                                                          >  1:3092318/1‑101 (MQ=255)
                                       gagaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGAAACCCGGGCGCCCGTTTTCTTTATCCtt                                                         >  2:2531650/1‑100 (MQ=255)
                                        agaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCGTGt                                                        <  1:2219787/101‑1 (MQ=255)
                                        agaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGGACCCGGGGCGCCGGTTTTTCTTATCCTGt                                                        >  1:83052/1‑101 (MQ=255)
                                        agaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.CGGGGGAACCCGGGCCGCCGTTTTTCATTTTCCTGt                                                        >  2:3013803/1‑101 (MQ=255)
                                         gaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGAACCCGGGCCGCCGTTTTTCATTATCCTGtt                                                       <  1:1758010/101‑1 (MQ=255)
                                         gaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGGACCCCGGGCCCCGTTTTTTCTTATccctgt                                                       >  2:1594645/1‑97 (MQ=255)
                                         gaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGAACCCGGGCCGCCGTTTTTTCTTTTCCTTtt                                                       >  1:3694517/1‑101 (MQ=255)
                                          aaGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGAAACCGGGCCGCCGTTTTTCATTATCCTGTTg                                                      >  2:3400202/1‑101 (MQ=255)
                                          aaGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGAAACCCGGCCGCCGTTTTTCATTATCCTGTTg                                                      >  1:4914605/1‑101 (MQ=255)
                                            gTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGATCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGAt                                                    <  2:2908416/101‑1 (MQ=255)
                                             ttCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGAACCCCGGGCGCCGTTTTTCATTATCCTGTTGATc                                                   >  1:4430803/1‑101 (MQ=255)
                                              tCAAGCCCTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCt                                                  <  1:1698414/101‑1 (MQ=255)
                                              tCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGGACCCGGGCCGCCCGTTTTCATTATCCTGTTGATCt                                                  >  2:3713711/1‑101 (MQ=255)
                                               cAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGACCCCGGGCCTCCGTTTTTCATTATCCTGTTGATCTg                                                 <  1:1057105/101‑1 (MQ=255)
                                                aaGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGAACCCGGGGCCCCGGTTTTCATTATCCTGTTGATCTgg                                                >  2:3941784/1‑101 (MQ=255)
                                                  gCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGAACCCGGGGCGCCGGTTTTTCTTATCCTGTTGATCTGGat                                              >  2:4507456/1‑101 (MQ=255)
                                                    ccTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGatat                                            <  1:1825901/100‑1 (MQ=255)
                                                    aCTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGatat                                            <  2:4589465/101‑1 (MQ=255)
                                                    aCTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGGAACCGGGCCGCCGGTTTTTATTATTCTTTTTATTTGGgtat                                            >  1:4700558/1‑101 (MQ=255)
                                                    aCTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGAACCCCGGCCGCCGGTTTTTATTATCCTCTTGATCTGGgtat                                            >  2:1027356/1‑101 (MQ=255)
                                                     cTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATg                                           <  1:4891518/101‑1 (MQ=255)
                                                     cTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATg                                           <  1:26595/101‑1 (MQ=255)
                                                      tGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGGACCCCGGCCGCCGTTTTTCATTTTCCTGTTGATCTGGGTATGt                                          >  1:662919/1‑101 (MQ=255)
                                                      tGCCACGGCCGTCGGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGt                                          <  2:1412492/101‑1 (MQ=255)
                                                       gCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGGACCCGGGCCGCCGCTTTTCATTATCCTGTTGATCTGGATATGtt                                         >  1:2486313/1‑101 (MQ=255)
                                                       gCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGAACCCGGGCCGCCGGTTTTTATTATCCTGTTGATCTGGGTATGtt                                         >  2:3276311/1‑101 (MQ=255)
                                                         cACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGtttt                                       <  1:3464295/101‑1 (MQ=255)
                                                          aCGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGttttt                                      <  2:4622143/101‑1 (MQ=255)
                                                           cGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTtg                                     <  1:3263077/101‑1 (MQ=255)
                                                           cGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTtg                                     <  2:2722944/101‑1 (MQ=255)
                                                             gCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTtgtg                                   <  2:1305479/101‑1 (MQ=255)
                                                             gCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTtttg                                   >  1:4757084/1‑101 (MQ=255)
                                                                gTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCG.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGaa                                <  1:3402952/101‑1 (MQ=255)
                                                                gTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGaa                                <  2:5021500/101‑1 (MQ=255)
                                                                gTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGAACCCGGGCCGCCGGTTTTCATTATCCTGTTGATCTGGATATGTTTTTTTGGaa                                >  2:3628492/1‑101 (MQ=255)
                                                                 tCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGaaa                               <  2:4993641/101‑1 (MQ=255)
                                                                  cTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAAt                              <  1:3832362/101‑1 (MQ=255)
                                                                  cTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTTTGGAAAt                              >  2:2643173/1‑101 (MQ=255)
                                                                  cTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGAAACCGGGCCGCCGGTTTTCATTTTCCTGTTGATCTTGATATGTTTTTTTGGAAAt                              >  1:4935186/1‑101 (MQ=255)
                                                                     gCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTc                           <  1:321499/101‑1 (MQ=255)
                                                                     gCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGAACCCCGGCCGCCGTTTTTCATTATCCTGTTGATCTTGATATGTTTTTTTGGAAAATTc                           >  2:4135515/1‑101 (MQ=255)
                                                                     gCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.CGGGGGAACCCGGGGCGGCGGTTTTCATTATCCCGTTGCTCTGGATATGTTTTTTTGGGAATTTc                           >  2:1229748/1‑101 (MQ=255)
                                                                     gCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGAACCCCGGCCGCCGTTTTTTATTATCCTGTTGATCTGGATATGTTTTTTTGGAAATTTc                           >  1:4101660/1‑101 (MQ=255)
                                                                       cTGAAACAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGTGAACCCCGGCCGCCGTTTTTCATTATTCCGTTGATCTGGATATGTTTTTTTGGAAATTTtcg                         >  2:3790247/1‑101 (MQ=255)
                                                                        tGAAACAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTccgg                        <  1:4967026/101‑1 (MQ=255)
                                                                         gAAACAAAGGCGCAGAAATATGGG.GGCGGTCC.GGGGGGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTccggc                       <  2:3161013/101‑1 (MQ=255)
                                                                          aaaCAATGGTGGAGAAATATGGG.GGCGCTCC.GCGTTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTccggcc                      <  2:3190287/101‑1 (MQ=255)
                                                                          aaaCAAAGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTccggcc                      <  1:1680213/101‑1 (MQ=255)
                                                                          aaaCAAAGGCGCTGAAATATGGC.GGCGGGCC.GGGGGGAAACCCGGCCGCCGGTTTTCATTATCCTGTTTATCTGGATATGTTTTTGTGGAAATTTccggcc                      >  2:1778108/1‑101 (MQ=255)
                                                                          aaaCAAAGGCGCTGAAATATGGC.GGCGGCCC.GGGGGGAACCCGCCCGGCCGTTTTTTTTTATTTTGTTTATTTGGATATGTTTTTTTTGATATTTccggcc                      >  1:2092212/1‑101 (MQ=255)
                                                                              aaaGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGc                  <  1:2067762/101‑1 (MQ=255)
                                                                              aaaGGCGCTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGc                  <  1:894687/101‑1 (MQ=255)
                                                                              aaaGGCGCTGAAATATGGC.GGCGGTCC.GGGGGGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGc                  >  2:2822671/1‑101 (MQ=255)
                                                                                aGGCGCTGAAATATGGC.GGCGGTCC.GGGTTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGa                <  2:4350835/101‑1 (MQ=255)
                                                                                 ggCGCGGAATTATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGaa               <  2:1337952/101‑1 (MQ=255)
                                                                                  gcggttaaaTATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAg              <  2:930625/95‑1 (MQ=255)
                                                                                    gcTAAAATAGGGG.GGCAGTCC.GGGGTGACCCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGt            <  1:3790532/101‑1 (MQ=255)
                                                                                     cTGAAATATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGtt           <  1:2822884/101‑1 (MQ=255)
                                                                                        aaaTATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTcc        <  2:1386740/101‑1 (MQ=255)
                                                                                        aaaTATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTcc        <  1:4816084/101‑1 (MQ=255)
                                                                                        aaaTATGGC.GGCGGTCC.GGGGGGAACCCGGGCCGCCGTTTTTTATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCCGCCGGGCGAAAGTTTcc        >  2:4727564/1‑101 (MQ=255)
                                                                                         aaTATGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCg       <  1:3850237/101‑1 (MQ=255)
                                                                                           tatGGC.GGCGGTCC.GGGGGGAACCCGGGCCGCCGTTTTTCATTTTTCTGTTGATCTGGGTATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGtt     >  1:79564/1‑101 (MQ=255)
                                                                                            atGGC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGGTTCCGttt    >  1:3652166/1‑101 (MQ=255)
                                                                                              ggC.GGCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCg  >  2:3423485/1‑101 (MQ=255)
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GAGCCCTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GCCGGTCC.GGGGTGAACCCGGGCCGCCGTTTTTCATTATCCTGTTGATCTGGATATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCG  >  NC_014034/256992‑257186

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG