Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,603,050	A→C	100%	E160A (GAG→GCG)	RCAP_rcc02427 →	M23 family peptidase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,603,050	0	A→C	100.0%	27.6	8	E160A (GAG→GCG)	RCAP_rcc02427	M23 family peptidase
Reads supporting (aligned to +/- strand):  new base (3/5):  ref base (0/0):  total (3/5)

GGGGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGAGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCGCCG  >  NC_014034/2602959‑2603142                                                                                            |                                                                                             ggggCGGCCGCCGCGGGCAGCGAGCGGATGGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCcgc                                                                                     <  2:1193560/101‑1 (MQ=255)       gccgccGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCCCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGcccc                                                                               <  2:1857832/101‑1 (MQ=255)         cgccgcGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCGGCCGGCGCCCGCGCCCCCCCCACCCACCCCCCCCCCGCGCCCCCCCCCGcccccc                                                                             >  2:146001/1‑100 (MQ=255)             gggggCGGCGAGCGGATCGACACCCCCCCGCGGGCGGGGCCCGCGATCGCCCGCGCCGCCGTCCCCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCAc                                                                         <  1:2847510/99‑1 (MQ=255)                    gcgagcgGATCGACATCCCCTCGCTGGGGGGGGCCGCGATCGACCGCCCCGCCGTCCCCCCGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGccc                                                                  <  1:3752195/101‑1 (MQ=255)                    gcgagcgGATCGACATCCCCCCGCGGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGccc                                                                  <  1:146017/101‑1 (MQ=255)                             tCGACATCCCCTCGCTGGCGGGGGCCGCGATCGCCCGCGCCGCCGTCCCCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCccgcc                                                         <  1:4832824/101‑1 (MQ=255)                                                        cgATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGaaa                              >  2:2329106/1‑101 (MQ=255)                                                                         gTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCa             >  1:4062956/1‑101 (MQ=255)                                                                                    gcccgccGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGCGgccg  >  1:2400670/1‑101 (MQ=255)                                                                                            |                                                                                             GGGGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGAGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCGCCG  >  NC_014034/2602959‑2603142 Base quality scores:  ATCG  < 3 ≤  ATCG  < 7 ≤  ATCG  < 16 ≤  ATCG  < 27 ≤  ATCG  < 39 ≤  ATCG