Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,731,802 G→C 100% P509A (CCC→GCC)  RCAP_rcc02540 ← diguanylate cyclase/phosphodiesterase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,731,8020G→C100.0% 699.3 185P509A (CCC→GCC) RCAP_rcc02540diguanylate cyclase/phosphodiesterase
Reads supporting (aligned to +/- strand):  new base (121/64):  ref base (0/0):  total (121/64)

Only 100 of 193 total aligned reads displayed.

CAGATCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCGGGGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCATGGCTGGC  >  NC_014034/2731705‑2731895
                                                                                                 |                                                                                             
cAGATCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcg                                                                                            >  1:4192631/1‑101 (MQ=255)
   aTCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcgcgc                                                                                         >  1:2988019/1‑101 (MQ=255)
   aTCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcgcgc                                                                                         <  1:2367401/101‑1 (MQ=255)
     cACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcgcgcgc                                                                                       >  2:2738540/1‑101 (MQ=255)
         cGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAg                                                                                   >  2:3133423/1‑101 (MQ=255)
          ggTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGc                                                                                  >  2:3302714/1‑101 (MQ=255)
           gTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGACGCGCGCGCGCGCAGCt                                                                                 >  2:4542448/1‑101 (MQ=255)
            tCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTg                                                                                <  1:4102622/101‑1 (MQ=255)
               cccAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTgcgc                                                                             >  2:3647891/1‑101 (MQ=255)
               cccAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTgcgc                                                                             >  1:3448060/1‑101 (MQ=255)
                 gctcTGCGGGCCGAAACGGTCTTTTATCTGCGGGAACTGGTCGATCGCCAGGAAGACCTGCTCCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCg                                                                           <  1:718827/98‑1 (MQ=255)
                   gCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCggg                                                                         >  1:4987022/1‑101 (MQ=255)
                   gCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCggg                                                                         >  1:1727537/1‑101 (MQ=255)
                   gCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCggg                                                                         >  1:781802/1‑101 (MQ=255)
                      gCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCg                                                                      >  1:4597251/1‑101 (MQ=255)
                        gggCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGAc                                                                    >  1:3068005/1‑101 (MQ=255)
                         ggCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGAcc                                                                   <  2:939401/101‑1 (MQ=255)
                          gCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCt                                                                  <  2:4757342/101‑1 (MQ=255)
                          gCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCt                                                                  <  2:227570/101‑1 (MQ=255)
                             gAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTggg                                                               >  2:3825023/1‑101 (MQ=255)
                              aaaCGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGgc                                                              >  2:3207230/1‑101 (MQ=255)
                              aaaCGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGgc                                                              <  2:2145364/101‑1 (MQ=255)
                              aaaCGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGgc                                                              >  2:2014252/1‑101 (MQ=255)
                               aggggTCCTTGCTCTGCGGGAACGGGGCGAGCGACAGGAAGACCTGCTGAAAGAGATCGCCCGC..CGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGcgcg                                                           <  1:4024377/98‑1 (MQ=255)
                                  ggTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCgg                                                          >  1:2752162/1‑101 (MQ=255)
                                  ggTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCgg                                                          >  1:3822382/1‑101 (MQ=255)
                                   gTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGa                                                         >  2:1087148/1‑101 (MQ=255)
                                    tCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGaa                                                        <  2:1761532/101‑1 (MQ=255)
                                       ttGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGa                                                     >  1:3920589/1‑101 (MQ=255)
                                       ttGATCTGCGGGAACTGGTCGATCGACAGGAAGACCTGCTGAAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGa                                                     <  1:5032865/101‑1 (MQ=255)
                                        ttaTCTGCTGGACCGGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGAt                                                    <  2:766488/99‑1 (MQ=255)
                                        tGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGAt                                                    >  1:3428299/1‑101 (MQ=255)
                                         gATCTGCTGGAACGGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGAtt                                                   <  2:1387699/101‑1 (MQ=255)
                                          aTCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTc                                                  <  2:2911919/101‑1 (MQ=255)
                                          aTCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTc                                                  <  2:3067767/101‑1 (MQ=255)
                                          aTCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTc                                                  <  1:3495587/101‑1 (MQ=255)
                                           tCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTcg                                                 <  2:1297803/101‑1 (MQ=255)
                                            ctgctgGAGCTGGTTGATCGACAGGAAGACCGTCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTcgc                                                <  1:2330456/101‑1 (MQ=255)
                                            ctgctgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTcgc                                                >  2:349407/1‑101 (MQ=255)
                                            ctgctgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTcgc                                                >  2:4391252/1‑101 (MQ=255)
                                              gctgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGa                                              >  2:3390864/1‑101 (MQ=255)
                                              gctgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGa                                              >  2:4020583/1‑101 (MQ=255)
                                                tgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGAtt                                            >  1:1664255/1‑101 (MQ=255)
                                                 ggAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGGAGGCGCGGCGCGCCGCCGTGGGCGGGGAAGGACTCGCGAGTg                                           >  2:909506/1‑101 (MQ=255)
                                                 ggAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTg                                           >  2:1979856/1‑101 (MQ=255)
                                                 ggAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTg                                           >  2:2607569/1‑101 (MQ=255)
                                                  gAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTgg                                          <  1:2670471/101‑1 (MQ=255)
                                                     cTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCa                                       >  1:2033946/1‑101 (MQ=255)
                                                     cTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCa                                       >  1:2927431/1‑101 (MQ=255)
                                                      tGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCCCCCTGGGCGCGGAAGGATTCGCGATTGGGCac                                      >  1:2347289/1‑100 (MQ=255)
                                                       ggTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGc                                     <  1:869632/101‑1 (MQ=255)
                                                        gTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGcc                                    <  2:1316785/101‑1 (MQ=255)
                                                         tcgatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGccc                                   >  1:2580501/1‑101 (MQ=255)
                                                         tcgatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGccc                                   >  1:2646081/1‑101 (MQ=255)
                                                         tcgatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGccc                                   <  1:4745302/101‑1 (MQ=255)
                                                          cgatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCg                                  >  1:3299681/1‑101 (MQ=255)
                                                          cgatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCg                                  <  1:1151929/101‑1 (MQ=255)
                                                           gatcgaCAGGAAAACCTGCTGCAAGAGATCGCCCGCCGCGGGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGTATTCGCGATTGAGCATGCCgg                                 >  2:4212924/1‑101 (MQ=255)
                                                            atcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGt                                >  1:752215/1‑101 (MQ=255)
                                                            atcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGt                                >  1:2458243/1‑101 (MQ=255)
                                                             tcgaCAGGAAGACCTGCTGCAGGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTAGCGATTGGGCAGCCCGGTc                               >  1:3724028/1‑101 (MQ=255)
                                                             tcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTc                               <  2:4596929/101‑1 (MQ=255)
                                                              cgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCa                              >  1:1950981/1‑101 (MQ=255)
                                                                 cAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGGTTCGCGATTGGGGAGCCCGGTCAgcg                           >  1:2286774/1‑101 (MQ=255)
                                                                 cAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAgcg                           >  1:4589108/1‑101 (MQ=255)
                                                                 cAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAgcg                           >  2:4724974/1‑101 (MQ=255)
                                                                 cAGAAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGTCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAgcg                           <  1:604076/101‑1 (MQ=255)
                                                                  aGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAgcgc                          <  1:2585163/101‑1 (MQ=255)
                                                                  aGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAgcgc                          >  2:4981016/1‑101 (MQ=255)
                                                                  aGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAgcgc                          >  1:4982052/1‑101 (MQ=255)
                                                                  aGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAgcgc                          >  2:2923773/1‑101 (MQ=255)
                                                                    gAAGCCCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCATCTGCTCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCTcat                        <  2:5002235/101‑1 (MQ=255)
                                                                    gAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGcat                        >  1:795160/1‑101 (MQ=255)
                                                                    gAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGcat                        >  1:4903427/1‑101 (MQ=255)
                                                                     aacacgtgctgcAAGAGCGCTCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCCACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGcatc                       <  2:2370247/95‑1 (MQ=255)
                                                                     aaGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGcatc                       >  1:4205216/1‑101 (MQ=255)
                                                                     aaGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGcatc                       <  2:3689120/101‑1 (MQ=255)
                                                                      aGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGcatca                      >  1:4213900/1‑101 (MQ=255)
                                                                          ctgctgCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCg                  <  1:3385143/101‑1 (MQ=255)
                                                                          ctgctgCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCg                  >  2:2806911/1‑101 (MQ=255)
                                                                            gctgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTc                >  1:102864/1‑101 (MQ=255)
                                                                            cttccgatctaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTc                <  1:4237189/91‑1 (MQ=255)
                                                                             ctgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTcg               >  2:4097364/1‑101 (MQ=255)
                                                                             ctgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTcg               >  1:3264612/1‑101 (MQ=255)
                                                                               gcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTcgcg             >  1:555930/1‑101 (MQ=255)
                                                                               gcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTcgcg             >  2:1756360/1‑101 (MQ=255)
                                                                               gcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTcgcg             >  1:4700245/1‑101 (MQ=255)
                                                                               gcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGGcgcg             >  2:409285/1‑101 (MQ=255)
                                                                                 aaGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGc           >  1:154397/1‑101 (MQ=255)
                                                                                  agagATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCa          >  1:914835/1‑101 (MQ=255)
                                                                                  agagATCGCCCGCCGCGCGCGCGCGCAGCAGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCa          >  2:3916579/1‑101 (MQ=255)
                                                                                    agaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtg        <  1:2098490/101‑1 (MQ=255)
                                                                                    agaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtg        >  1:1018306/1‑101 (MQ=255)
                                                                                      aTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTcagatcggaag      >  2:4236872/1‑91 (MQ=255)
                                                                                      aTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggc      <  2:173309/101‑1 (MQ=255)
                                                                                        cGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggctg    >  1:1231737/1‑101 (MQ=255)
                                                                                         gCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATNCGTcgcgnnnnnnnnnn   >  2:1497708/1‑91 (MQ=255)
                                                                                         gCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggctgg   >  1:1271156/1‑101 (MQ=255)
                                                                                         gCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggctgg   >  2:624659/1‑101 (MQ=255)
                                                                                          cccGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggctggc  <  1:2014396/101‑1 (MQ=255)
                                                                                                 |                                                                                             
CAGATCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCGGGGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCATGGCTGGC  >  NC_014034/2731705‑2731895

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG