Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,740,181 G→T 10.1% G453C (GGC→TGC)  RCAP_rcc02544 → ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,740,1810G→T10.1% 3.6 ‑2.8 ‑0.047G453C (GGC→TGC) RCAP_rcc02544ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (22/20):  total (27/20)
Fisher's exact test strand distribution p-value = 6.27e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.66e-01

Only 100 of 134 total aligned reads displayed.

GCTCGATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCTGATCGTC  >  NC_014034/2740081‑2740281
                                                                                                      |                                                                                                     
gcTCGATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.g                                                                                                       <  2:1657077/101‑1 (MQ=255)
gcTCGATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.g                                                                                                       <  1:4280813/101‑1 (MQ=255)
  tCGATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.TGc                                                                                                     >  1:1725173/1‑101 (MQ=255)
  tCGATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGc                                                                                                     >  1:2411657/1‑101 (MQ=255)
  ccGATGCCCGTCTTGGCCGGGTGCTCCTGGTCGAGACCACCCCCGAGGCGGCTCTGGCGGCGGCGCCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGc                                                                                                     <  2:1620281/100‑1 (MQ=255)
   cGATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGcc                                                                                                    >  1:4682783/1‑101 (MQ=255)
    gATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCcg                                                                                                   >  2:1292572/1‑101 (MQ=255)
       gCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGGGACCGACTGGGGCC.GGATCAACGCCCCGCCT.TGGCgcgc                                                                                                >  1:3453557/1‑101 (MQ=255)
        cccGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCCGCgcc                                                                                               <  2:315734/101‑1 (MQ=255)
        cccGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCCGCgcc                                                                                               <  1:3151962/101‑1 (MQ=255)
        cccGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCCGCgcc                                                                                               <  1:913097/101‑1 (MQ=255)
         ccGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.TGGCgcgcgc                                                                                              >  2:1239782/1‑99 (MQ=255)
         ccGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCCGCgcca                                                                                              <  1:1097600/101‑1 (MQ=255)
            tCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGGCGCgcccgcc                                                                                           >  1:2086597/1‑101 (MQ=255)
            tCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCCGCgccagcc                                                                                           <  2:1375158/101‑1 (MQ=255)
            gcTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCCGCgccagcc                                                                                           <  2:1041029/100‑1 (MQ=255)
              ttGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.TGTCGCccccgccca                                                                                         >  2:4623106/1‑99 (MQ=255)
              ttGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCCGCGccagccag                                                                                         <  1:4246020/101‑1 (MQ=255)
               tGGCCGGGTGCTCTCGGGCGAGATCGCCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCCCCGACCGGCTT.GGCCGCGCcagccagc                                                                                        <  2:55464/101‑1 (MQ=255)
                ggCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGGGACCGACTGGGACC.GGATCACCGACCGGCTT.GCGCCCGCccgcccgcc                                                                                       >  1:4203944/1‑100 (MQ=255)
                ggCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.TGCCCCgcccccccccc                                                                                       >  1:1035312/1‑93 (MQ=255)
                   cGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCCGCCCCCGCCCGc.gccc                                                                                   >  2:2146672/1‑100 (MQ=255)
                    nggTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.TGTCGCGCCCCCCCNc.gncnc                                                                                  >  1:439786/2‑101 (MQ=255)
                    gggTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.TGGCGCGCCCGCCCGc.gccgc                                                                                  >  1:46008/1‑101 (MQ=255)
                    gggTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCCGCGCCAGCCAGc.gccgc                                                                                  <  2:2670941/101‑1 (MQ=255)
                     ggTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCCGCGCCAGCCAGC.gccgcc                                                                                 <  2:2738720/101‑1 (MQ=255)
                     ggTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCCGCGCCAGCCAGC.gccgcc                                                                                 <  1:3280019/101‑1 (MQ=255)
                       tGCTCGCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCCGCGCCAGCCAGC.GCCGCCAc                                                                               <  2:3056343/101‑1 (MQ=255)
                        gCTCTGGGGCGAGATCCCCCCCGAGGCGGCTCGGGCGGCGGCGGCGCCCGACTGGGACC.GTATCCCCGCCCGGCTT.GGCCGCGCCCGCCAGC.GCCGCCACt                                                                              <  2:332936/101‑1 (MQ=255)
                        gCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGAGCACCGACCGGCTT.TGGCGCCGCCGCCGGC.gcccccccc                                                                              >  1:2188886/1‑98 (MQ=255)
                          cccggggCGAGACCCCCCCGGAGGCGGCTCGGGCGGCGGCGGCGACCGACTGGGACC.GGATCCCCGCCCGGCTT.GGCCGCCCCAGCCAGC.GCCGCCACTAc                                                                            <  2:3699780/97‑1 (MQ=255)
                             cGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.TGGCGCGCCCGGCAGC.gccccccccccccc                                                                         >  1:4938463/1‑93 (MQ=255)
                             cGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.TGGCGCGCCCGCCCGC.GCCCCCCCCAcccc                                                                         >  2:556604/1‑99 (MQ=255)
                              gggCGAGATCCCCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGACCCCCGCCCGGCTT.GCCCGCGCCAGCCAGC.GCCGCCACTACcgcg                                                                        <  1:1858506/101‑1 (MQ=255)
                              gggCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.TGCCGCGCCCGCCCCC.cccgccccccccccc                                                                        >  1:897059/1‑92 (MQ=255)
                              gggCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCCGCGCCAGCCAGC.GCCCCCACTACcgcg                                                                        >  1:735298/1‑101 (MQ=255)
                                gCGAGATCGCCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCCCCGCCCGGCTT.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAg                                                                      <  1:3083512/101‑1 (MQ=255)
                                 cGAGACCCCCCCGGGGGCGGCTCGGGCGGCGGGGGGGCCCGACGGGGCCG.GGATCCCCGCCCGTCTT.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAgg                                                                     <  2:3425905/101‑1 (MQ=255)
                                  gagaTCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.TGGCGCGCCCCCCCGC.GCCCCCCCCACCCCGatcg                                                                    >  1:2774947/1‑98 (MQ=255)
                                  gagaTCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.TGCCGCGCCCGCCAGC.GCCGCCCCTACCCCGCggg                                                                    >  2:1949550/1‑100 (MQ=255)
                                   agagcccccccGGGTGGGCTCGGGGGGCGGCGGGACCCGAGGGGAACC.GGAGAACCGGCGGGCTG.GCCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCg                                                                   <  1:5002763/95‑1 (MQ=255)
                                   agaccccccccGGGGCGGCTCGGGCGGCGGCGGCGACCGACGGGGACC.GGACCCCCGCCCGGCTG.GGCCGCCCCAGCCAGC.GCCGCCACTACCGCGAGGCg                                                                   <  1:1340955/95‑1 (MQ=255)
                                     aTCCCCCCCGGGGCGGCTCGGGCGGGGGGGGCGCCCGACTGGGCCC.GGATCCCCGCCCGGCTT.GCCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGAt                                                                 <  2:2511669/101‑1 (MQ=255)
                                      cccccccgGGGGCGGCTCTGGCGGCGGGGGCGACCGCCTGGGCCC.GGATCCCCGCCCGGCTG.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATg                                                                <  2:480225/98‑1 (MQ=255)
                                        cccccgGGGGCGGCTGGGGCGGGGGGGGCGACCAGCGGGGACG.GGACCCCCGCCGGGCTG.GGCCGCGCCACCCAGC.GCCGCCACTACCGCGAGGCGATggg                                                              <  1:4048540/100‑1 (MQ=255)
                                        ccccccGGGGCGCCTCGGGCGGCGGCGGCGCCCGACGGGGACC.GGATCCCCGCCCGGCTT.GCCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATggg                                                              <  2:3874197/100‑1 (MQ=255)
                                         ccccgGGGGCGGCTCGGGGGGCGGGGGCGACCGACGGGGCCC.GGACCCCCGCCCGGCTG.GGCCGCCCCAGCCAGC.GCCGCCACTACCGCGAGGCGATgggg                                                             <  1:3667928/101‑1 (MQ=255)
                                          ccgcGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCCGCGCCCGCCAGC.GCCCCCACCACCGCCAGGCGGTGGGGc                                                            >  1:2884317/4‑101 (MQ=255)
                                            ccGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCCGCGCCCGCCAGC.GCCGCCCCCACCGCGAGGCGATGGGGCTg                                                          >  1:264312/1‑101 (MQ=255)
                                             cggggcggcTCGGGGGGGGGCGGCGACCGACTGGGCCC.GGATCCCCGCCCGGCTT.GCCCGCCCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTgg                                                         <  2:4068870/98‑1 (MQ=255)
                                             cGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGGTGGGGGTgg                                                         >  2:1262071/1‑101 (MQ=255)
                                               aGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.TGCCGCGCCCAGCAGC.GCCGCCGCCACCCCCGGGCGGTgggggggggg                                                       >  2:927162/1‑95 (MQ=255)
                                                ggcggcTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.TTCCGCGCCCGCCAGC.GCCCCCCCCACCCCGGGGGGGGGGGGCGGGGgg                                                      >  2:1531699/1‑101 (MQ=255)
                                                ggcggcTCGGGGGGCGGGGGCGACCGACGGGGACC.GGACCACCCACGGGTTG.GGCCGCCCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAgg                                                      <  1:826258/101‑1 (MQ=255)
                                                 gggcctcgGGGGGCGGCGGCGCCCG.CCGGGACCGGGATCCCCGCCCGGCTT.GGCCGCCCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAggc                                                     <  1:1262192/97‑1 (MQ=255)
                                                       ctGGGGGGGGCGGCGCCCGCGGGGGCCC.GGACCCCCCACCGGCTG.GGCCGCGCCGGCCGGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGcc                                               <  1:4772492/101‑1 (MQ=255)
                                                       ctGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.TGTCGCGCCCCCCAGC.GCCGCCCCCCCCCCCAGGCGAGGGGGGGGGGGGCGgggcg                                               >  2:1604086/1‑100 (MQ=255)
                                                       ctGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCCGCGCCAGCCAGC.GCCGCCCCTACCGCGAGGCGATGGGGCTGGAGGGGGCGcc                                               >  1:3251705/1‑101 (MQ=255)
                                                       cgggcggcggcggcGCCCGACGGGGCCC.GGATCACCGCCCGGCTG.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGcc                                               <  2:3006116/99‑1 (MQ=255)
                                                        tGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCCGCGCCAGCCAGC.GCCCCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCa                                              >  1:2502594/1‑101 (MQ=255)
                                                         ggcggcggcggcGACCGACTGGGACC.GGATCACCGACCGGCTT.TTGCGCGCCAGCCCGCGGCCGCC.CCCCCCCCCGGCGATGGGGCGGGAggcgggggcct                                             >  2:280281/1‑95 (MQ=255)
                                                         ggcggcggcggcGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCACCGCCACCCAGC.GCCGCCTCTACCGCGAGACGATGGGGCGGGGGGGCGCGCCag                                             >  2:2863025/1‑100 (MQ=255)
                                                          ggggcggcggcgCCCGACTGGGCCC.GGATCCCCGCCCGGCTG.GCCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATg                                            <  1:3708808/99‑1 (MQ=255)
                                                          gcgggggcggcgACCGACGGGGCCC.GGACCCCCGCCCGGCTG.GCCCGCCCCAGCCAGC.TCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATg                                            <  2:2576094/101‑1 (MQ=255)
                                                            ggcggcggcgCCCGACTGGGCCC.GGACCACCGCCCGGCTG.GGCCGCCCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAg                                          <  1:1280872/101‑1 (MQ=255)
                                                              ggggggcgACGGACGGGGACC.GGACCCCCGCCCGGCTG.GGCCGCCCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGcc                                        <  2:4405481/97‑1 (MQ=255)
                                                                gcggcgACCGGGTGGGACC.GGACCCCGGACCGGCTT.GCCCGCGCCGGCCGGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCtg                                      <  2:639549/101‑1 (MQ=255)
                                                                 gggcgCCCGCCGGGGCCC.GGACCCCCGCCGGGCTG.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCtgt                                     <  1:1381588/100‑1 (MQ=255)
                                                                  ggcgCCCGCCTGGGCCC.GGACCCCCGACGGGTTG.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCtgtg                                    <  1:2514913/101‑1 (MQ=255)
                                                                  ggcgACCGACTGGGACC.GGATCACCGACCGGCTT.GGCCGCGCCCGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCACGAGcccggg                                    >  1:502424/1‑97 (MQ=255)
                                                                   gcaaccggcGGGGAGC.GGACCCCGGCCGGGCTG.GCCCGCCCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTgg                                   <  2:472738/98‑1 (MQ=255)
                                                                    cgcccgacGGGCCCC.GGACCCCCGCCCGGCTG.GCCCGCCCCACCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGgc                                  <  1:4541173/98‑1 (MQ=255)
                                                                    cgaccgacTGGGACC.GGATCACCGACCGGCTT.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTggg                                  >  1:2752456/1‑100 (MQ=255)
                                                                     gaccgacGGGGACC.GGACCCCCGCCCGGCTG.GCCCGCCCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGgcg                                 <  1:172581/101‑1 (MQ=255)
                                                                         gacTGGGACC.GGATCACCGACCGGCTT.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGc                             >  1:4413059/1‑101 (MQ=255)
                                                                         ctgtGGGCCG.GGACCCCCGCCCGGCTT.GGCCGCCCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGc                             <  1:3308926/98‑1 (MQ=255)
                                                                           cTGGGACC.GGATCACCGACCGGCTT.TGCCGCGCCAGGCAGC.GCCGCCCCCACCGCGAGGCGCTGGGGCTGGGGGCGGGGCCATGAGCCCGTGGCGCGGcgg                           >  2:3308664/1‑99 (MQ=255)
                                                                           cTGGGACC.GGATCACCGACCGGCTT.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGCGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTCGGGCGCgggct                           >  1:1592828/1‑98 (MQ=255)
                                                                            tGGGACC.GGATCACCGACCGGCTT.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGGAtt                          >  2:1937518/1‑101 (MQ=255)
                                                                            ggccccc.GGACCCCCGCCCGGCTG.GCCCGCCCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCAtt                          <  2:972636/96‑1 (MQ=255)
                                                                             gggACC.GGATCACCGACCGGCTT.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTc                         >  1:2285644/1‑101 (MQ=255)
                                                                              ggccc.GGATCCCCGCCGGGTTG.GGCCGCCCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCa                        <  1:2891505/98‑1 (MQ=255)
                                                                              ggACC.GGATCACCGACCGGCTT.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCa                        >  2:4136473/1‑101 (MQ=255)
                                                                                    ggATCACCGACCGGCTT.GGCCGCCCCCGGCAGG.CCCCCCCCTACCGCGCGGCGATGGGGGCGGGGGCGGGGGCCTGAGCCGGTGGCGCGGccgtccagggg                   >  2:1640344/1‑91 (MQ=255)
                                                                                    cggaccccggccgGGTTGGGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAgggg                    <  2:218730/95‑1 (MQ=255)
                                                                                     gATCACCGACCGGCTT.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGGGGGGGGGCCCAGAGCCCGTGGCGCGGGCTTCAgggggg                  >  2:3498716/1‑99 (MQ=255)
                                                                                      aTCACCGACCGGCTT.TGGCGCGCCCGCCAGC.GCCGCCACCACCCCGAGGCGGTGGGGCTGGGGGCGGCGCCCTGAGGCTGTGGCGCGGCATTCAGGggggg                 >  2:3061342/1‑101 (MQ=255)
                                                                                       cccccgcccgGCTT.GCCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGggcggc                <  1:1239899/98‑1 (MQ=255)
                                                                                        cACCGACCGGCTT.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGggcggcc               >  1:2579948/1‑100 (MQ=255)
                                                                                         cccgccgggCTT.CCCCGCGCCAGCCCGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTg              <  2:1169385/94‑1 (MQ=255)
                                                                                            cgccggctggggCCCCGCCACCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGtt            <  2:3942727/91‑1 (MQ=255)
                                                                                             cccgGCGT.GCCCCCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGc          <  1:2336942/100‑1 (MQ=255)
                                                                                              cgggCTG.GCCCGCCCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCt         <  2:363425/99‑1 (MQ=255)
                                                                                              ccgGCTT.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCt         >  1:1482967/1‑101 (MQ=255)
                                                                                                 gCTT.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCTGAt      >  2:3905735/1‑101 (MQ=255)
                                                                                                  cTT.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCTGAtc     >  2:1353763/1‑101 (MQ=255)
                                                                                                  cTT.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCTGAtc     >  1:2055126/1‑101 (MQ=255)
                                                                                                   tt.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCTGAtcg    >  1:2826323/1‑101 (MQ=255)
                                                                                                    t.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCTGGtcgt   >  2:1529329/1‑101 (MQ=255)
                                                                                                      ggCCGCCCCCGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCTGAtcgtc  <  2:2001704/101‑1 (MQ=255)
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GCTCGATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACC.GGATCACCGACCGGCTT.GGCCGCGCCAGCCAGC.GCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCTGATCGTC  >  NC_014034/2740081‑2740281

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG