Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,854	C→G	100%	intergenic (‑186/+107)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,854	0	C→G	100.0%	79.3	27	intergenic (‑186/+107)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (9/18):  ref base (0/0):  total (9/18)

ATAGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGC  >  NC_014034/2747760‑2747950                                                                                                |                                                                                                  aTAGGAACCCCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCgaga                                                                                              <  2:2092251/101‑1 (MQ=25) aTAGGAAACGCGCCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCgaga                                                                                              <  2:3085245/101‑1 (MQ=37)  tAGGAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGaa                                                                                             <  1:4643178/101‑1 (MQ=37)  tAGGAACCCCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCCGCGCCCGGCCCGGCGGGCGAGaa                                                                                             <  1:4064584/101‑1 (MQ=25)   aGGAACCGCGCCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAg                                                                                            <  2:4846053/101‑1 (MQ=37)   aGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAg                                                                                            >  2:3724733/1‑101 (MQ=255)    gaaaaCCCGCCCCGCTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgc                                                                                           <  1:3725011/99‑1 (MQ=25)       accgcgCCCCGGTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcc                                                                                        <  2:3423423/99‑1 (MQ=21)       accgcgCCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcc                                                                                        <  1:3750972/99‑1 (MQ=25)       accgcgCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcc                                                                                        <  1:3859787/99‑1 (MQ=25)       aaCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcc                                                                                        <  1:4016287/101‑1 (MQ=25)        ccgcgACCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgccc                                                                                       <  2:561100/100‑1 (MQ=25)         cgcgCCCCGGTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcccg                                                                                      <  2:3978245/101‑1 (MQ=21)         cgcgACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcccg                                                                                      >  1:4888539/1‑101 (MQ=255)          ccgACCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcccgc                                                                                     <  1:4443637/100‑1 (MQ=25)               ccgttttcccccGCGCGCCGCGCGCTGTTGCGGCATGACCGGTCCAGCCCGCACCACAGAGGGCAGCGCCGGGCCCGGCGGGCGAGAAGCGCCCgcc.gggc                                                                               <  2:2278308/93‑3 (MQ=12)                cggctccccccGCCCGCAGCGCCGGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                              <  2:4979845/94‑4 (MQ=21)                 ccgTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                             <  2:251635/100‑5 (MQ=25)                 ccccccccccGCCCGCAGCCCCCGTTTGCGGCATGCCCGCTTCAGCCCGCCCCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                             <  1:1793972/95‑5 (MQ=18)                    ttcccccGCCCGCAGCGCCCGGTTGCGGCATGCCCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccgggc                                                                          <  2:3005922/98‑8 (MQ=21)                    ttcccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccgggc                                                                          <  1:4834653/98‑8 (MQ=21)                                         gTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCCGCCCGAGGGCCTTTGCGc                                                      >  2:880316/1‑101 (MQ=18)                                                                            ggCGGCGCCCGCCCCGGCGGGCGAGATGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGcc                   <  1:3227835/101‑1 (MQ=17)                                                                                      ggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg         >  2:2821873/1‑101 (MQ=18)                                                                                      ggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg         >  2:1751191/1‑101 (MQ=18)                                                                                        cccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCATAGGACCTCTGTGACGCATGGTCAAGGCGATGGcc       >  2:4209726/5‑101 (MQ=18)                                                                                            gcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGGGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTgg   >  2:4059751/1‑101 (MQ=21)                                                                                            gcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCTCAGGACCTCTGTGACGCCTGGCTAAGGCGATGGCCCTgg   >  2:328431/1‑101 (MQ=18)                                                                                             cgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGc  >  2:2560535/1‑101 (MQ=21)                                                                                             cgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGc  >  2:1207904/1‑101 (MQ=21)                                                                                                |                                                                                                  ATAGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGC  >  NC_014034/2747760‑2747950 Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG