Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,891,981 T→G 100% S460A (TCG→GCG)  thiP → thiamine ABC transporter permease

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,891,9810T→G100.0% 760.0 202S460A (TCG→GCG) thiPthiamine ABC transporter permease
Reads supporting (aligned to +/- strand):  new base (110/92):  ref base (0/0):  total (110/92)

Only 100 of 210 total aligned reads displayed.

CCGGCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCTCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGCCTTT  >  NC_014034/2891884‑2892080
                                                                                                 |                                                                                                   
  ggCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTg                                                                                                >  1:1485618/1‑101 (MQ=255)
    cTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGtt                                                                                              <  2:146871/101‑1 (MQ=255)
     tGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGttt                                                                                             <  1:3351315/101‑1 (MQ=255)
     tGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGttt                                                                                             >  2:1155841/1‑101 (MQ=255)
      gCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGtttt                                                                                            >  1:2926637/1‑101 (MQ=255)
      gCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGtttt                                                                                            <  1:770003/101‑1 (MQ=255)
       cGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCCGTTTTc                                                                                           >  2:916960/1‑101 (MQ=255)
         gTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCg                                                                                         >  2:3175013/1‑101 (MQ=255)
         gTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCg                                                                                         <  1:2469186/101‑1 (MQ=255)
          ttCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGa                                                                                        >  1:3510593/1‑101 (MQ=255)
           aaatCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGAt                                                                                       >  2:3898170/4‑101 (MQ=255)
            ccTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATg                                                                                      >  2:3487986/1‑101 (MQ=255)
            ccTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATg                                                                                      >  2:2859033/1‑101 (MQ=255)
               cACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGcc                                                                                   <  2:1223231/101‑1 (MQ=255)
                aCCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGGGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCg                                                                                  >  1:4276773/1‑101 (MQ=255)
                aCCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCg                                                                                  >  2:272038/1‑101 (MQ=255)
                aCCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCg                                                                                  >  1:4827385/1‑101 (MQ=255)
                  ccTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCa                                                                                <  2:428744/101‑1 (MQ=255)
                  ccTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCa                                                                                <  2:3740630/101‑1 (MQ=255)
                  ccTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCa                                                                                >  2:1674960/1‑101 (MQ=255)
                   cTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAg                                                                               <  1:1902266/101‑1 (MQ=255)
                   cTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAg                                                                               <  2:5054259/101‑1 (MQ=255)
                   cTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAg                                                                               <  1:3568089/101‑1 (MQ=255)
                     gCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGcc                                                                             <  1:3975687/101‑1 (MQ=255)
                     gCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGcc                                                                             >  1:3732319/1‑101 (MQ=255)
                     gCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGcc                                                                             >  2:1657863/1‑101 (MQ=255)
                       cccGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGGTGGCCGCAGCCAg                                                                           >  1:930289/1‑101 (MQ=255)
                         cGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGAc                                                                         <  1:621846/101‑1 (MQ=255)
                            cTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCt                                                                      >  1:269516/1‑101 (MQ=255)
                            cTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCt                                                                      <  1:3045756/101‑1 (MQ=255)
                             tGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTg                                                                     >  1:857951/1‑101 (MQ=255)
                             tGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTg                                                                     <  2:4827058/101‑1 (MQ=255)
                              ggCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCAGCAGCCAGACCCTGc                                                                    >  1:457787/1‑101 (MQ=255)
                                    gCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTg                                                              >  2:3082276/1‑101 (MQ=255)
                                    gCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTg                                                              <  2:4654622/101‑1 (MQ=255)
                                     ccGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGc                                                             <  1:4901446/101‑1 (MQ=255)
                                      cGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGcc                                                            >  1:3191370/1‑101 (MQ=255)
                                       gACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCg                                                           >  2:3011841/1‑101 (MQ=255)
                                       gACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCg                                                           >  1:1214800/1‑101 (MQ=255)
                                        aCGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCTTCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGt                                                          >  1:2131951/1‑101 (MQ=255)
                                        aCGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGt                                                          >  1:4877614/1‑101 (MQ=255)
                                        aCGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGt                                                          >  1:23383/1‑101 (MQ=255)
                                         cGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTc                                                         <  2:457722/101‑1 (MQ=255)
                                         cGGGCTTTGCCCCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGTGCCATCGCGCTGTTTTCCGATGGCCGCAGCCCGACCCTGCCGCTTGCCggc                                                         >  1:2967705/1‑99 (MQ=255)
                                           ggCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTa                                                       <  1:447862/101‑1 (MQ=255)
                                             cTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATc                                                     <  2:3460165/101‑1 (MQ=255)
                                             cTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATc                                                     <  2:1102668/101‑1 (MQ=255)
                                              tttGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCa                                                    <  1:2260660/101‑1 (MQ=255)
                                               ttGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAg                                                   >  1:122403/1‑101 (MQ=255)
                                                   cgcgGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGa                                               <  2:139407/101‑1 (MQ=255)
                                                    gcgGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGAt                                              >  1:2915356/1‑101 (MQ=255)
                                                      ggggATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATgg                                            <  2:633362/101‑1 (MQ=255)
                                                      ggggATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATgg                                            >  1:1555339/1‑101 (MQ=255)
                                                        ggATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGc                                          >  2:4423162/1‑101 (MQ=255)
                                                         gATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCa                                         >  2:378447/1‑101 (MQ=255)
                                                          aTTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAg                                        <  1:1104378/101‑1 (MQ=255)
                                                            tGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCt                                      <  2:2043330/101‑1 (MQ=255)
                                                             gCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTa                                     >  1:3818447/1‑101 (MQ=255)
                                                             gCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTa                                     <  2:4485008/101‑1 (MQ=255)
                                                              ccGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTAt                                    <  1:3634739/101‑1 (MQ=255)
                                                               cgcgGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATc                                   <  2:258060/101‑1 (MQ=255)
                                                               cgcgGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATc                                   >  1:4347918/1‑101 (MQ=255)
                                                                gcgGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCg                                  <  2:4103180/101‑1 (MQ=255)
                                                                 cgGCTTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGc                                 <  2:4188512/101‑1 (MQ=255)
                                                                 cgGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGc                                 <  2:4614910/101‑1 (MQ=255)
                                                                  ggCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCa                                >  1:4987344/1‑101 (MQ=255)
                                                                    ccTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATg                              <  1:3072390/101‑1 (MQ=255)
                                                                     cTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATgg                             <  1:2767796/101‑1 (MQ=255)
                                                                       tttCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGAt                           >  1:517085/1‑101 (MQ=255)
                                                                       tttCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGAt                           >  2:233402/1‑101 (MQ=255)
                                                                        ttCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATc                          <  2:4554585/101‑1 (MQ=255)
                                                                        ttCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATc                          <  1:1797277/101‑1 (MQ=255)
                                                                           gATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAgg                       <  2:2647136/101‑1 (MQ=255)
                                                                           gATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAgg                       >  2:2554306/1‑101 (MQ=255)
                                                                            aTGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGc                      <  1:4131846/101‑1 (MQ=255)
                                                                            aTGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGc                      >  1:1030929/1‑101 (MQ=255)
                                                                            aTGGGCGACCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGc                      >  1:2118604/1‑101 (MQ=255)
                                                                              gggCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCcg                    <  2:3722549/101‑1 (MQ=255)
                                                                               ggCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCcgc                   <  1:2859246/101‑1 (MQ=255)
                                                                                 cGATCTGGGCGCCATCGCGCTGTTTTCCGATGTCCGCAGCCAGACCCTGCCGCTTCCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCgg                 <  1:4704721/101‑1 (MQ=255)
                                                                                 cGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCgg                 >  1:245884/1‑101 (MQ=255)
                                                                                  gATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCggg                >  1:2776143/1‑101 (MQ=255)
                                                                                  gATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCggg                >  2:3270483/1‑101 (MQ=255)
                                                                                   aTCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGgc               <  2:3360251/101‑1 (MQ=255)
                                                                                    tCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGgcg              >  2:4044881/1‑101 (MQ=255)
                                                                                     cTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGgcgc             >  1:1983514/1‑101 (MQ=255)
                                                                                     cTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGgcgc             >  1:3882698/1‑101 (MQ=255)
                                                                                      tGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGcgcg            >  1:1290514/1‑101 (MQ=255)
                                                                                      tGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGcgcg            >  2:3850884/1‑101 (MQ=255)
                                                                                      tGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGcgcg            >  2:3579498/1‑101 (MQ=255)
                                                                                      tGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGcgcg            >  1:1832030/1‑101 (MQ=255)
                                                                                      tGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCAAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGCCAGCTATCGCATGGATCAGGCCGCGGGcgcg            >  1:3715450/1‑101 (MQ=255)
                                                                                       gggCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCgg           >  2:672025/1‑101 (MQ=255)
                                                                                       gggCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCgg           >  2:298009/1‑101 (MQ=255)
                                                                                         gcgcCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCt         <  2:269482/101‑1 (MQ=255)
                                                                                           gcCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTg       <  1:4085687/101‑1 (MQ=255)
                                                                                           gcCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTg       <  2:1305507/101‑1 (MQ=255)
                                                                                             cATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGcc     >  1:465979/1‑101 (MQ=255)
                                                                                             cATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGcc     <  2:2269925/101‑1 (MQ=255)
                                                                                              aTCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGCCt    <  1:4599762/101‑1 (MQ=255)
                                                                                                 |                                                                                                   
CCGGCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCTCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGCCTTT  >  NC_014034/2891884‑2892080

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG