Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,950,754 T→G 3.3% intergenic (+97/‑63) RCAP_rcc02761 → / → RCAP_rcc02762 glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,950,7540T→G 3.3% 17.6 ‑1.1 ‑0.2305intergenic (+97/‑63)RCAP_rcc02761/RCAP_rcc02762glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand):  new base (7/3):  ref base (156/139):  total (163/142)
Fisher's exact test strand distribution p-value = 3.47e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.63e-01

Only 100 of 334 total aligned reads displayed.

CTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGG  >  NC_014034/2950654‑2950854
                                                                                                       |                                                                                                     
cTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.ttt                                                                                                       >  2:128358/1‑101 (MQ=255)
 tGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTg                                                                                                      >  1:4999302/1‑101 (MQ=255)
   aagcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGcc                                                                                                    <  1:1151524/101‑1 (MQ=255)
      caagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAA.TTTGCCGTc                                                                                                 >  2:3461095/1‑101 (MQ=255)
        agcCGGGGGCTCTGCCCCCGGACCCCCGGGGTATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTATCCCCAAATATCCC.GGGGGCAGA.TTTGCCGTCAg                                                                                               >  1:4340486/1‑101 (MQ=25)
        agcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAg                                                                                               <  2:4635137/101‑1 (MQ=255)
          ccGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGc                                                                                             <  2:2298986/101‑1 (MQ=255)
           cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.a                                                                                           <  1:4407770/101‑1 (MQ=255)
           cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.a                                                                                           <  1:1444175/101‑1 (MQ=255)
           cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAA.TTTGCCGTCAGGC.a                                                                                           >  2:3521124/1‑101 (MQ=255)
            gggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.aa                                                                                          >  1:2306517/1‑101 (MQ=255)
             ggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.aaa                                                                                         >  1:791269/1‑101 (MQ=255)
             ggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.aaa                                                                                         <  1:104303/101‑1 (MQ=255)
             ggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.aaa                                                                                         <  2:630936/101‑1 (MQ=255)
                gCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAagcg                                                                                      >  2:3276200/1‑99 (MQ=255)
                 ctctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAgg                                                                                     <  2:2819560/101‑1 (MQ=255)
                   gtGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAA.CTCGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAgggg                                                                                   <  1:1260861/100‑1 (MQ=255)
                   ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAgggg                                                                                   >  1:4202894/1‑101 (MQ=255)
                    tGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAggggg                                                                                  <  1:3425636/101‑1 (MQ=255)
                     ttcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAA.CCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAA.TTGGCCG.AAGGC.CAAGAggggggc                                                                                >  2:1893938/9‑99 (MQ=25)
                       cgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAA.CCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAA.TTGGCCG.AAGGC.CAAGAggggggcag                                                                              >  1:4647411/7‑97 (MQ=25)
                        tcgctccccccGGGATATTTGCGGCAAGATGAAG.TGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAA.TTGGCC.AAAGGC.CAAGAGGGGGCAGcg                                                                             >  2:2550520/7‑100 (MQ=2)
                         ttccgatctcGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTT.ATCTTGCCTCAGATATCCC.GGGGGTGAATTTTGGCG.CAGCCAAAAGAGGGGGCAGc                                                                              <  2:3081419/92‑1 (MQ=21)
                         ccGGACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCt                                                                             >  2:1846072/1‑101 (MQ=255)
                           ggACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTcc                                                                           >  1:1514369/1‑101 (MQ=255)
                            gACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTccc                                                                          >  2:3032138/1‑101 (MQ=255)
                            cccccccGGGATATTTGCGGCAAGATGAAG.GGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAA.TTGGCC.AAAGGC.CAAGAGGGGGCAGCGcccc                                                                         <  2:4480383/99‑1 (MQ=0)
                             ccccccGGGATATTTATGGCAAGATGAAA.CACGCCTCCTTCATCTTGCCATGAATATTCC.GGGGGTGAC.TTGCCCGGCAGGG.CCAGAGGGCGcgnggggcc                                                                         >  1:1925338/2‑93 (MQ=21)
                              cccccGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTccccc                                                                        <  2:4186523/101‑1 (MQ=255)
                              cccccGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTccccc                                                                        <  2:1651424/101‑1 (MQ=255)
                               ccccGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCt                                                                       <  2:4375291/101‑1 (MQ=255)
                               ccccGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCt                                                                       <  2:1774606/101‑1 (MQ=255)
                                  cGGGATATTTGGGGCAAGGTAAAAGCATTGCCCTTT.ATCTTGCCTCAGATATCCC.GGGGGTGAATTTTGGCG.CAGCCAAAAGAGGGGGCAGcagatcggaa                                                                     >  1:3081657/1‑92 (MQ=21)
                                  cGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGcc                                                                    >  2:2488397/1‑101 (MQ=255)
                                   gggATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGccc                                                                   >  2:2166833/1‑101 (MQ=255)
                                    ggATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGcccc                                                                  >  2:3705574/1‑101 (MQ=255)
                                     gATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCg                                                                 <  1:4034401/101‑1 (MQ=255)
                                     gATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCg                                                                 <  1:510810/101‑1 (MQ=255)
                                        atTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCt                                                              >  1:353690/1‑101 (MQ=255)
                                         tttGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTg                                                             <  1:413145/101‑1 (MQ=255)
                                          ttGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTga                                                            >  1:4260655/1‑101 (MQ=255)
                                            gAGGCAAGATGAAA.CCCGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagg                                                          <  2:4711811/101‑1 (MQ=255)
                                             aGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggg                                                         >  1:3656143/1‑101 (MQ=255)
                                             aGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggg                                                         <  1:1713012/101‑1 (MQ=255)
                                               gCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCgagatcggaag                                                       >  2:4053570/1‑91 (MQ=255)
                                               gCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagggag                                                       >  1:3027214/1‑101 (MQ=255)
                                                cAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagggagg                                                      >  1:1977678/1‑101 (MQ=255)
                                                cAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagggagg                                                      <  1:2048791/101‑1 (MQ=255)
                                                  aGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCg                                                    >  2:3633830/1‑101 (MQ=255)
                                                   gATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCgg                                                   >  1:582926/1‑101 (MQ=255)
                                                   gATGAAA.CACGCCCCTTTAATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCgg                                                   >  2:4239675/1‑101 (MQ=255)
                                                     tGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa                                                 >  2:3804543/1‑101 (MQ=255)
                                                       aaa.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACt                                               <  1:667267/101‑1 (MQ=255)
                                                         a.cacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTgg                                             <  2:4768018/101‑1 (MQ=255)
                                                           cacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGa                                            >  1:941723/1‑101 (MQ=255)
                                                           cacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGa                                            <  1:316308/101‑1 (MQ=255)
                                                           cacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGa                                            >  1:4740256/1‑101 (MQ=255)
                                                             cGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaaa                                          >  1:2510591/1‑101 (MQ=255)
                                                              gCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGATGGAGGCGGAACTGGAAAc                                         >  1:4526904/1‑101 (MQ=255)
                                                                cccTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCt                                       <  1:3276452/101‑1 (MQ=255)
                                                                 ccTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCtt                                      <  2:238200/101‑1 (MQ=255)
                                                                 ccTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCtt                                      >  2:89712/1‑101 (MQ=255)
                                                                 ccTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCtt                                      >  2:1055890/1‑101 (MQ=255)
                                                                   tttCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGa                                    <  1:189152/101‑1 (MQ=255)
                                                                   tttCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGa                                    <  1:802106/101‑1 (MQ=255)
                                                                     tCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAg                                  >  1:574923/1‑101 (MQ=255)
                                                                      cATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGa                                 >  2:3583859/1‑101 (MQ=255)
                                                                      cATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGa                                 >  1:2968022/1‑101 (MQ=255)
                                                                      cATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGa                                 >  2:3184527/1‑101 (MQ=255)
                                                                      cATCTTGCCCCAAATATCCC.GGGGGGGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGCAGa                                 >  1:2456924/1‑101 (MQ=255)
                                                                          ttGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCg                             >  2:4941542/1‑101 (MQ=255)
                                                                             ccccAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCt                          <  1:3898042/101‑1 (MQ=255)
                                                                              cccAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTg                         <  1:1782707/101‑1 (MQ=255)
                                                                                cAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGAt                       >  1:2821660/1‑101 (MQ=255)
                                                                                 aaaTATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                      <  1:37619/101‑1 (MQ=255)
                                                                                 aaaTATCCC.GGGGGTGAA.TTTGCC.TCCGGC.AAAGGGGGGGGCGCCCCCCCTGCCCCGGCCGGGGGGGGGGGGACCGGAAACCCTGAAGGCCCCCCCGATcc                     >  1:4819043/1‑100 (MQ=255)
                                                                                  aaTATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg                     >  2:2453297/1‑101 (MQ=255)
                                                                                  aaTATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCCGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg                     <  1:2250968/101‑1 (MQ=255)
                                                                                   atgtCCC.GGGTGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGa                    <  1:4774660/98‑1 (MQ=255)
                                                                                   atatCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGa                    >  1:2168663/1‑101 (MQ=255)
                                                                                   atatCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGa                    <  1:3577038/101‑1 (MQ=255)
                                                                                   atatCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGa                    >  2:1968682/1‑101 (MQ=255)
                                                                                    agtgggggggggTGTT.TTGGCCGAAAGGA.AAGGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGa                    <  1:4590811/94‑1 (MQ=255)
                                                                                     atCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAag                  >  1:1141106/1‑101 (MQ=255)
                                                                                       ccc.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAagag                <  2:1514239/101‑1 (MQ=255)
                                                                                        cc.GGGGGTGAT.TTTGCTGTAAGGG.AAGGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAgg               <  2:544161/101‑1 (MQ=255)
                                                                                        cc.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAgg               >  2:3370641/1‑101 (MQ=255)
                                                                                           gggtgTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc             >  1:4801744/1‑101 (MQ=255)
                                                                                           gggggTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc             >  2:5004739/1‑101 (MQ=255)
                                                                                           gggggTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc             >  2:2613178/1‑101 (MQ=255)
                                                                                           gggggTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc             >  2:1260014/1‑101 (MQ=255)
                                                                                            ggggTTAA.TTTGCCGCCAGGA.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGccc            <  2:511392/101‑1 (MQ=255)
                                                                                            ggggTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGccc            <  1:2671444/101‑1 (MQ=255)
                                                                                             gggTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcg           <  2:40697/101‑1 (MQ=255)
                                                                                                 gAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcgcgcg       >  1:3785915/1‑101 (MQ=255)
                                                                                                   tcactGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGa      >  2:2949339/5‑101 (MQ=255)
                                                                                                   a.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGAGac     <  2:4555230/101‑2 (MQ=255)
                                                                                                     tttGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc    <  2:791167/101‑1 (MQ=255)
                                                                                                       tGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCgg  <  2:2881868/101‑1 (MQ=255)
                                                                                                       tGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCgg  >  2:3891611/1‑101 (MQ=255)
                                                                                                       |                                                                                                     
CTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAA.CACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAA.TTTGCCGTCAGGC.AAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGG  >  NC_014034/2950654‑2950854

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG