Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 297,560 A→G 100% K12E (AAG→GAG)  RCAP_rcc00260 → alanine racemase domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034297,5600A→G100.0% 888.4 235K12E (AAG→GAG) RCAP_rcc00260alanine racemase domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (146/89):  ref base (0/0):  total (146/89)

Only 100 of 242 total aligned reads displayed.

GGGCCTTGGCCCGCTTCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGAAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCTCGATCA  >  NC_014034/297461‑297657
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gggCCTTGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAg                                                                                                 >  1:2523377/1‑101 (MQ=255)
  gCCTTGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGc                                                                                               <  1:4366192/101‑1 (MQ=255)
  gCCTTGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGc                                                                                               <  1:1650539/101‑1 (MQ=255)
    cTTGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGCTTGCAGGAGATTCAGACCCGGATTGCGGAGGCGa                                                                                             >  2:2505189/1‑101 (MQ=255)
    cTTGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGa                                                                                             >  1:3141556/1‑101 (MQ=255)
     ttGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGAc                                                                                            <  1:3198796/101‑1 (MQ=255)
      tGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGAcc                                                                                           <  2:3884659/101‑1 (MQ=255)
       ggCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGGGAccc                                                                                          >  2:3225043/1‑101 (MQ=255)
       ggCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGAccc                                                                                          >  1:1971090/1‑101 (MQ=255)
       ggCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGAccc                                                                                          <  2:1166477/101‑1 (MQ=255)
              ctcttttctttTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccg                                                                                    >  2:1059735/2‑101 (MQ=255)
               tcttttctttTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgc                                                                                   >  2:3533943/1‑101 (MQ=255)
               tcttttctttTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgc                                                                                   <  2:1098500/101‑1 (MQ=255)
                  tttcttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcggg                                                                                >  1:4041649/1‑101 (MQ=255)
                  tttcttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcggg                                                                                >  2:2771992/1‑101 (MQ=255)
                   ttcttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggc                                                                               >  2:3891739/1‑101 (MQ=255)
                    tcttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggcc                                                                              >  1:3066219/1‑101 (MQ=255)
                    tcttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggcc                                                                              >  1:461228/1‑101 (MQ=255)
                     cttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggccg                                                                             >  1:3024851/1‑101 (MQ=255)
                     cttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggccg                                                                             >  1:1478089/1‑101 (MQ=255)
                     cttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggccg                                                                             >  1:3227504/1‑101 (MQ=255)
                       tttttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGcgggccggg                                                                           >  1:4666828/1‑101 (MQ=255)
                       tttttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGcgggccggg                                                                           >  2:4174436/1‑101 (MQ=255)
                        ttttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGcgggccgggc                                                                          >  1:2375532/1‑101 (MQ=255)
                         tttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCgggccgggcc                                                                         >  1:817166/1‑101 (MQ=255)
                          ttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGggccgggccg                                                                        <  2:2546917/101‑1 (MQ=255)
                          ttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGggccgggccg                                                                        >  1:1034116/1‑101 (MQ=255)
                           tGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGa                                                                       >  1:3938266/1‑101 (MQ=255)
                           tGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGa                                                                       >  2:1622986/1‑101 (MQ=255)
                            gCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAg                                                                      >  1:3089875/1‑101 (MQ=255)
                               aCCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAgggg                                                                   >  2:3652185/1‑101 (MQ=255)
                               aCCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAgggg                                                                   >  1:4899173/1‑101 (MQ=255)
                               aCCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAgggg                                                                   >  2:1231741/1‑101 (MQ=255)
                                ccTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAggggg                                                                  >  2:3373409/1‑101 (MQ=255)
                                ccTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAggggg                                                                  >  2:2150208/1‑101 (MQ=255)
                                   tgtgCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCgg                                                               >  1:1350320/1‑101 (MQ=255)
                                   tgtgCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCgg                                                               >  1:2554683/1‑101 (MQ=255)
                                    gtgCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCggg                                                              >  1:2481185/1‑100 (MQ=255)
                                    gtgCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGt                                                              <  2:2630024/101‑1 (MQ=255)
                                    gtgCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGt                                                              <  1:870546/101‑1 (MQ=255)
                                      gCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGc                                                            >  1:2686962/1‑101 (MQ=255)
                                      gCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGc                                                            <  1:4922917/101‑1 (MQ=255)
                                         cGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGc                                                         >  2:232783/1‑101 (MQ=255)
                                          gATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCt                                                        >  2:189221/1‑101 (MQ=255)
                                           aTTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGGGGGGGCGGTGCGGCTg                                                       >  1:1864260/1‑101 (MQ=255)
                                            ttGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGa                                                      <  2:4379507/101‑1 (MQ=255)
                                             tGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGAt                                                     >  1:3486367/1‑101 (MQ=255)
                                              gcgcCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATc                                                    <  2:2871592/101‑1 (MQ=255)
                                                 ccgcAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGcc                                                 <  2:4660863/101‑1 (MQ=255)
                                                  cgcAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCg                                                >  2:931214/1‑101 (MQ=255)
                                                  cgcAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCg                                                <  1:4985340/101‑1 (MQ=255)
                                                   gcAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCgt                                               >  1:3760958/1‑101 (MQ=255)
                                                   gcAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCgt                                               >  1:2770508/1‑101 (MQ=255)
                                                    cAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCgtg                                              >  2:3475976/1‑101 (MQ=255)
                                                     aaaGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCgtgt                                             >  1:1693516/1‑101 (MQ=255)
                                                      aaGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGTGGGCGGGGCGGCTGATCGCCGTGTc                                            >  1:4227390/1‑101 (MQ=255)
                                                      aaGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTc                                            <  2:3764444/101‑1 (MQ=255)
                                                       aGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGGGGCTGATCGCCGTGTCg                                           >  2:2403743/1‑101 (MQ=255)
                                                         gagaTCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGaa                                         >  2:3698283/1‑101 (MQ=255)
                                                            aTCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAggg                                      >  2:1721067/1‑100 (MQ=255)
                                                             tCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTg                                     <  2:1517752/101‑1 (MQ=255)
                                                              cggcATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGc                                    <  2:143991/98‑1 (MQ=255)
                                                              cGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGc                                    <  2:4877311/101‑1 (MQ=255)
                                                               gCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCa                                   >  2:4023963/1‑101 (MQ=255)
                                                               gCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCa                                   >  2:2111795/1‑101 (MQ=255)
                                                                ccATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAg                                  <  2:1477959/101‑1 (MQ=255)
                                                                 cATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAgc                                 >  1:4139405/1‑101 (MQ=255)
                                                                   tGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAgccg                               <  2:2342446/101‑1 (MQ=255)
                                                                    gggATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAgccgc                              >  1:1617721/1‑101 (MQ=255)
                                                                    gggATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAgccgc                              <  1:1507039/101‑1 (MQ=255)
                                                                      gATTGCAGGAGATTCAGACCCGGATTGCGGGGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGccc                            >  1:4493449/1‑101 (MQ=255)
                                                                      gATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGccc                            <  2:3107651/101‑1 (MQ=255)
                                                                      gATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGccc                            <  1:4434055/101‑1 (MQ=255)
                                                                      gATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGccc                            <  2:577276/101‑1 (MQ=255)
                                                                       aTTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCg                           <  1:3534213/101‑1 (MQ=255)
                                                                       aTTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCg                           >  2:2022726/1‑101 (MQ=255)
                                                                       aTTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCg                           <  2:4397109/101‑1 (MQ=255)
                                                                        ttGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGa                          >  2:3591331/1‑101 (MQ=255)
                                                                             ggAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCggg                     >  1:3855307/1‑101 (MQ=255)
                                                                              gagaTTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCgggg                    >  2:4968213/1‑100 (MQ=255)
                                                                              gagaTTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGGGCAGCCGCCCGAGCGGGt                    >  2:782008/1‑101 (MQ=255)
                                                                               agaTTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTg                   <  2:4898846/101‑1 (MQ=255)
                                                                               agaTTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTg                   >  2:4819494/1‑101 (MQ=255)
                                                                                gaTTCAGACCCGGATTGCGGGGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGc                  <  1:3633784/101‑1 (MQ=255)
                                                                                gaTTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGc                  >  2:5050672/1‑101 (MQ=255)
                                                                                   tCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGGGCTgg               >  1:944064/1‑101 (MQ=255)
                                                                                    cAGACCCGGATTGCGGAGGCGCCCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGc              <  1:4117313/101‑1 (MQ=255)
                                                                                    cAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGc              <  2:432656/101‑1 (MQ=255)
                                                                                    cAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGc              <  1:2344853/101‑1 (MQ=255)
                                                                                     aGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCg             >  1:4784901/1‑101 (MQ=255)
                                                                                     aGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCg             >  2:3438470/1‑101 (MQ=255)
                                                                                       aCCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGt           <  2:1923601/101‑1 (MQ=255)
                                                                                          cGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCt        <  2:461163/101‑1 (MQ=255)
                                                                                           ggATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCTc       <  2:3937974/101‑1 (MQ=255)
                                                                                           ggATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCTc       >  2:4589144/1‑101 (MQ=255)
                                                                                            gATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCTCg      <  1:3970351/101‑1 (MQ=255)
                                                                                             aTTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGGGCTCGa     >  1:609082/1‑101 (MQ=255)
                                                                                             aTTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGGGCTGGCGGTGCTCGa     >  1:3372109/1‑101 (MQ=255)
                                                                                               tGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGCGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGcccacgc   >  2:3387058/1‑95 (MQ=255)
                                                                                                gCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCTCGATCa  <  1:1043485/101‑1 (MQ=255)
                                                                                                   |                                                                                                 
GGGCCTTGGCCCGCTTCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGAAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCTCGATCA  >  NC_014034/297461‑297657

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG