Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,053 A→C 13.7% intergenic (+99/+77) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0530A→C13.7% 101.0 ‑0.3 ‑1.9282intergenic (+99/+77)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (23/16):  ref base (132/109):  total (156/126)
Fisher's exact test strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.32e-02

Only 100 of 305 total aligned reads displayed.

GAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTC  >  NC_014034/3003953‑3004153
                                                                                                      |                                                                                                    
gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCa                                                                                                      >  1:2230103/1‑101 (MQ=255)
gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCa                                                                                                      >  2:3112332/1‑101 (MQ=255)
 aGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCGCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCAc                                                                                                     <  1:4556203/101‑1 (MQ=255)
 aGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCAc                                                                                                     <  1:788213/101‑1 (MQ=255)
  gggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCAcc                                                                                                    <  2:2352751/101‑1 (MQ=255)
    gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCtt                                                                                                  <  1:4511320/101‑1 (MQ=255)
    gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCtt                                                                                                  <  1:2295134/101‑1 (MQ=255)
     cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTg                                                                                                 <  1:264199/101‑1 (MQ=255)
       aGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGgc                                                                                               >  1:4629299/1‑101 (MQ=255)
        ggCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGgcg                                                                                              <  2:3732028/101‑1 (MQ=255)
           ggggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCaa                                                                                           <  1:363600/101‑1 (MQ=255)
            gggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCaaa                                                                                          >  2:810863/1‑101 (MQ=255)
            gggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCaaa                                                                                          <  1:1503321/101‑1 (MQ=255)
             ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                                         <  2:3794527/101‑1 (MQ=255)
              gggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTCTGCCTTTCACCTTGGCGCAAata                                                                                        <  1:4063349/101‑1 (MQ=255)
               ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                                       <  2:4732737/101‑1 (MQ=255)
                gcgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATc                                                                                      <  2:3589046/101‑1 (MQ=255)
                 cgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATcc                                                                                     <  1:4646721/101‑1 (MQ=255)
                 cgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATcc                                                                                     <  2:2379454/101‑1 (MQ=255)
                  gcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCACGGTGAAAGCAG.A.TCCCCGTTTGCCTTTCACCTTGGCGCAAATATccc                                                                                    >  2:2150991/1‑101 (MQ=255)
                   cTGCCCCCCTCTTGGCCTGACGGCCAATTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCg                                                                                   <  1:2482718/101‑1 (MQ=255)
                   cTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCg                                                                                   <  2:452483/101‑1 (MQ=255)
                            tctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTc                                                                          <  1:2359152/101‑1 (MQ=255)
                             ctTGGCCTGCCGGCCAATTCCCCCCCCGGGATAGTTGGACCAAGGTGAAAGCAG.C.TCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCCGGGGGAGTcc                                                                         <  1:4367089/101‑1 (MQ=25)
                             ctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTcc                                                                         <  2:2958657/101‑1 (MQ=255)
                              ttGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTccc                                                                        <  2:3930688/101‑1 (MQ=255)
                               tGGCCTGACGGCCAATTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                                       <  1:1672037/101‑1 (MQ=255)
                               tGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                                       >  2:156278/1‑101 (MQ=255)
                                 gCCTGACGGCCAATTCACCCCCCGGGATATTTTGACCAAGGGGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCCAATATCCCGGGGGGGGTCCcccg                                                                     >  1:1032845/1‑101 (MQ=25)
                                  ccTGACGGCCAATTCACCCCCCGGGTCATTTGGACCAAGGCGAAAGCAG.A.TCCCGGTTTGCCATTCACCTTCGCGCAAATAACCCGGGGGGGGCCCCGCgg                                                                    >  1:2733077/1‑101 (MQ=255)
                                  ccTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgg                                                                    >  1:4203133/1‑101 (MQ=255)
                                   cTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTTGCGCAAATATCCCGGGGGGAGTCCCGCggg                                                                   >  2:203495/1‑101 (MQ=255)
                                      aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAc                                                                >  2:71604/1‑101 (MQ=255)
                                      aCGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAc                                                                <  1:1376598/101‑1 (MQ=255)
                                       cGGCCAATTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATCTCCCGGGGGGAGTCCCGCGGGGACg                                                               <  1:289254/101‑1 (MQ=255)
                                        ggCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgg                                                              <  2:3511420/101‑1 (MQ=255)
                                         gCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggg                                                             >  1:2123343/1‑101 (MQ=255)
                                         gCCAATTCACCCACCGGGCTATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggg                                                             >  2:4098059/1‑101 (MQ=255)
                                          ccAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggg                                                            >  1:3217725/1‑101 (MQ=255)
                                           cAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggg                                                           >  2:3024614/1‑101 (MQ=255)
                                           cAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggg                                                           >  2:1771986/1‑101 (MQ=255)
                                             aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                         <  1:3382853/101‑1 (MQ=255)
                                              ttCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                                        >  2:1685256/1‑101 (MQ=255)
                                              ttCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                                        >  1:2936243/1‑101 (MQ=255)
                                                cACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                                      >  2:3394055/1‑101 (MQ=255)
                                                cACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                                      <  2:2412091/101‑1 (MQ=255)
                                                  ccccccGGGATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgc                                                    >  1:2083646/1‑101 (MQ=18)
                                                   ccccgGGGATATTTGGCCCAAGGGGAAAGCCG.C.GCCCCGCGTCCATTCCCCCTGGGCGGAAAGATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                                   <  1:3412249/101‑1 (MQ=17)
                                                    ccccGGGATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccc                                                  >  2:4675800/1‑101 (MQ=18)
                                                     cccTGGATATTTGGCCCAAGGTTAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGTAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccc                                                 <  2:4190271/101‑1 (MQ=17)
                                                       cGGGATATTTGGCCCAAGGTGAAAGCCGCG.CCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc                                               >  1:4015797/1‑101 (MQ=18)
                                                       cGGGATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc                                               <  1:3309940/101‑1 (MQ=17)
                                                       cGGGATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc                                               <  2:2832019/101‑1 (MQ=17)
                                                        gggATATTTGGGCCAAGGGGAAAGCAG.A.TCCCGTTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                              <  1:1948026/101‑1 (MQ=255)
                                                        gggATATTTGGCCCAAGGTGAAAGCCGCG.CCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccc                                              >  1:2173829/1‑100 (MQ=18)
                                                        gggATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGCGCAGAgcccccct                                              <  2:443392/101‑1 (MQ=255)
                                                         ggATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                             <  1:4107497/101‑3 (MQ=17)
                                                         ggATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctg                                             >  1:2404218/1‑101 (MQ=255)
                                                         ggATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctg                                             <  2:2632357/101‑1 (MQ=255)
                                                          gATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctc                                            >  1:492849/1‑98 (MQ=18)
                                                          gATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctc                                            >  2:3204991/1‑98 (MQ=18)
                                                          gATATTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctc                                            >  2:1171050/1‑98 (MQ=18)
                                                           atatTTGGCCCAAGGTGAAAGCCGCG.CCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                           >  1:5046354/1‑97 (MQ=18)
                                                           atatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                           >  1:3002992/1‑97 (MQ=18)
                                                           atatTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                           <  2:2228324/101‑5 (MQ=17)
                                                            tatTTGGCCCAAGGTGAAAGCCGCG.CCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                                          >  1:4303113/1‑96 (MQ=18)
                                                            tatTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                                          >  1:959254/1‑101 (MQ=255)
                                                             atTTGGCCCAAGGTGAAAGCCGCG.CCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                                         >  1:4767140/1‑95 (MQ=18)
                                                             atTTGGCCCAAGGTGAAAGCCG.C.GCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                                         <  2:3338605/101‑7 (MQ=17)
                                                             atTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                                         >  1:3194230/1‑101 (MQ=255)
                                                               ttGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                                       >  1:1808213/1‑101 (MQ=255)
                                                                tGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                                      >  1:704175/1‑101 (MQ=255)
                                                                 ggACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                                     >  2:1409343/1‑101 (MQ=255)
                                                                  gACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgg                                    <  1:614998/101‑1 (MQ=255)
                                                                  gACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgg                                    >  1:1251351/1‑101 (MQ=255)
                                                                   aCCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                                   >  1:245442/1‑101 (MQ=255)
                                                                   aCCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                                   >  1:1645031/1‑101 (MQ=255)
                                                                      aaGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                                >  2:2978307/1‑101 (MQ=255)
                                                                       aGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                               >  2:2881731/1‑101 (MQ=255)
                                                                       aGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                               >  2:1045559/1‑101 (MQ=255)
                                                                           gAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcg                           >  1:1285392/1‑101 (MQ=255)
                                                                           gAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcg                           >  2:163888/1‑101 (MQ=255)
                                                                            aaaGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgc                          <  2:1589702/101‑1 (MQ=255)
                                                                            aaaGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgc                          >  2:4848597/1‑101 (MQ=255)
                                                                              aGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTc                        >  2:2164143/1‑101 (MQ=255)
                                                                               gCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCa                       <  2:3708487/101‑1 (MQ=255)
                                                                                cAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                      >  1:4175298/1‑101 (MQ=255)
                                                                                      tCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgt                  <  1:3725010/101‑1 (MQ=255)
                                                                                       ccgggTTTGCCTTTCACCTGGGCGAAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtg                 <  2:2535146/98‑1 (MQ=255)
                                                                                       cccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtg                 >  2:648491/1‑101 (MQ=255)
                                                                                       cccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtg                 >  1:173725/1‑101 (MQ=255)
                                                                                       cccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtg                 <  1:3777667/101‑1 (MQ=255)
                                                                                         cGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt               >  2:232745/1‑101 (MQ=255)
                                                                                          ggTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGttt              >  1:1538321/1‑101 (MQ=255)
                                                                                           gTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg             >  2:1312060/1‑101 (MQ=255)
                                                                                           gTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg             <  2:546797/101‑1 (MQ=255)
                                                                                            tttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg            >  2:4986052/1‑101 (MQ=255)
                                                                                               gCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGa         >  2:786867/1‑101 (MQ=255)
                                                                                                ccTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAg        <  2:4791675/101‑1 (MQ=255)
                                                                                                 cTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCCAGCGCTCAGGCGTGTTTGGCGCGc       >  2:2739693/1‑101 (MQ=255)
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GAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.A.TCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTC  >  NC_014034/3003953‑3004153

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG