Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,101 A→C 14.6% intergenic (+147/+29) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,1010A→C14.6% 87.3 ‑0.4 ‑0.2239intergenic (+147/+29)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (22/13):  ref base (135/69):  total (157/82)
Fisher's exact test strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.92e-01

Only 100 of 267 total aligned reads displayed.

CACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTGGCGCAGCGGAT  >  NC_014034/3004001‑3004201
                                                                                                     |                                                                                                     
cACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                                                                                       >  2:3394055/1‑101 (MQ=255)
cACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                                                                                       <  2:2412091/101‑1 (MQ=255)
  ccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.c                                                                                                    <  1:4106316/101‑1 (MQ=255)
  ccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.c                                                                                                    <  2:3122348/101‑1 (MQ=255)
  ccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.c                                                                                                    <  1:1649886/101‑1 (MQ=255)
     cccTGGATATTTGGCCCAAGGTTAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGTAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.cccc                                                                                                 <  2:4190271/101‑1 (MQ=17)
     cccGGGATATTTGGAGTAAGGGGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccc                                                                                                 <  2:1571650/101‑1 (MQ=255)
      ccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccc                                                                                                <  1:2530452/101‑1 (MQ=17)
       cGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.cccccc                                                                                               <  1:3309940/101‑1 (MQ=17)
       cGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.cccccc                                                                                               <  2:2832019/101‑1 (MQ=17)
        gggATATTTGGGCCAAGGGGAAAGCAG.ATCCCGTTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccct                                                                                              <  1:1948026/101‑1 (MQ=255)
        gggATATTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccccc                                                                                              >  1:2173829/1‑100 (MQ=18)
        gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccct                                                                                              >  1:2268875/1‑101 (MQ=255)
        gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccct                                                                                              >  1:1258644/1‑101 (MQ=255)
         ggATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccccct                                                                                             >  1:2142267/1‑99 (MQ=18)
         ggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccctg                                                                                             >  1:3947349/1‑101 (MQ=255)
          gATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccccctc                                                                                            <  2:2167930/101‑4 (MQ=17)
          gATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccccctc                                                                                            >  1:492849/1‑98 (MQ=18)
          gATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccccctc                                                                                            >  1:4136885/1‑98 (MQ=18)
          gATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccctgc                                                                                            >  2:3828208/1‑101 (MQ=255)
           atatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccccctct                                                                                           >  2:2965004/1‑97 (MQ=18)
           atatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccctgcc                                                                                           >  2:2797563/1‑101 (MQ=255)
            tatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccctgccc                                                                                          >  2:3655992/1‑101 (MQ=255)
             atTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGATATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccccctcttc                                                                                         <  1:5028211/101‑7 (MQ=17)
             atTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCg.ccccccctcttc                                                                                         <  2:3338605/101‑7 (MQ=17)
             atTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccctgcccc                                                                                         <  2:2229948/101‑1 (MQ=255)
               ttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccctgcccccc                                                                                       >  1:1808213/1‑101 (MQ=255)
                tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAg.cccccctgcccccct                                                                                      >  2:1035704/1‑101 (MQ=255)
                  gACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTgg                                                                                    <  1:424257/101‑1 (MQ=255)
                   aCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTggg                                                                                   >  1:4045842/1‑101 (MQ=255)
                   aCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTggg                                                                                   <  2:106933/101‑1 (MQ=255)
                   aCCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTggg                                                                                   >  1:245442/1‑101 (MQ=255)
                    ccAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTgggg                                                                                  <  2:1629725/101‑1 (MQ=255)
                      aaGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGcc                                                                                <  2:2347252/101‑1 (MQ=255)
                       aGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCg                                                                               <  2:2729242/101‑1 (MQ=255)
                         gTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAg                                                                             >  2:2155355/1‑101 (MQ=255)
                         gTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAg                                                                             >  1:684806/1‑101 (MQ=255)
                           gAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAgcg                                                                           >  1:3194271/1‑101 (MQ=255)
                           gAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAgcg                                                                           >  2:1352043/1‑101 (MQ=255)
                            aaaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAgcgc                                                                          <  1:4612391/101‑1 (MQ=255)
                              aGCAG.ATCCCGGTTTGCCTTTCGCCTTCGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTc                                                                        <  2:5045843/101‑1 (MQ=255)
                              aGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTc                                                                        <  2:3490992/101‑1 (MQ=255)
                              aGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTc                                                                        >  2:1428570/1‑101 (MQ=255)
                               gCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCa                                                                       <  1:2445449/101‑1 (MQ=255)
                                cAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                                                                      >  2:1917209/1‑101 (MQ=255)
                                  g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc                                                                    >  2:2282945/1‑101 (MQ=255)
                                      ccgggTTTGCCTTTCACCTGGGCGAAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtg                                                                 <  2:2535146/98‑1 (MQ=255)
                                      cccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtg                                                                 >  1:173725/1‑101 (MQ=255)
                                      cccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtg                                                                 >  2:648491/1‑101 (MQ=255)
                                      cccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtg                                                                 <  1:3777667/101‑1 (MQ=255)
                                        cGGTTTGCCTTTCGCCTGGGGGCAAATGTCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGGGCTCAGGCGTGtt                                                               <  1:2911944/101‑1 (MQ=25)
                                         ggTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGttt                                                              >  1:1538321/1‑101 (MQ=255)
                                          gTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg                                                             <  2:546797/101‑1 (MQ=255)
                                           tttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTgg                                                            >  2:4986052/1‑101 (MQ=255)
                                            ttGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGc                                                           <  1:3112570/101‑1 (MQ=255)
                                             tGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGTCCGAGCGCTCATGCGTGTTTGGCg                                                          <  2:680179/101‑1 (MQ=255)
                                              gCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGa                                                         >  2:786867/1‑101 (MQ=255)
                                                cTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGc                                                       >  2:4534529/1‑101 (MQ=255)
                                                cTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGGGTTTGGCGAGc                                                       >  1:1357396/1‑101 (MQ=255)
                                                  ttCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCg                                                     >  1:2121982/1‑101 (MQ=255)
                                                      ccTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGctct                                                 >  1:1989616/1‑101 (MQ=255)
                                                       cTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCtt                                                <  2:4697266/101‑1 (MQ=255)
                                                          ggCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGa                                             >  2:1546401/1‑101 (MQ=255)
                                                          ggCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCCCTCAGGCGTGGTTGGCGAGCCGCTCTTCGa                                             >  1:465013/1‑101 (MQ=255)
                                                              cAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCAc                                         >  1:738824/1‑101 (MQ=255)
                                                               aaaTATCCGGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACg                                        <  1:3024852/101‑1 (MQ=255)
                                                               aaaTATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACg                                        >  2:4789000/1‑101 (MQ=255)
                                                               aaaTATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACg                                        >  1:713902/1‑101 (MQ=255)
                                                                aaTATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGt                                       >  1:1310550/1‑101 (MQ=255)
                                                                  tatCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCg                                     >  1:3452690/1‑101 (MQ=255)
                                                                  tatCCCGGGGGGAATCCCGCGGGGAAGGGGGGCAGAG.CAACCCTGCCCCCCTGGGGCCCAGCGCTCAGGCGCGTTTGGCGAGCCGCTCTTCGAGCACGTCg                                     >  1:2468932/1‑101 (MQ=255)
                                                                   atCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGa                                    >  1:4254292/1‑101 (MQ=255)
                                                                   atCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAA.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGa                                    >  1:2764113/1‑101 (MQ=255)
                                                                     gcgggggggGGTCCCGGGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAg                                  <  1:906734/98‑1 (MQ=255)
                                                                       cGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAggg                                >  1:1121842/1‑101 (MQ=255)
                                                                        ggggggAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGc                               >  2:1671275/1‑101 (MQ=255)
                                                                        ggggggAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGc                               >  1:2959710/1‑101 (MQ=255)
                                                                          ggggAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCAc                             <  1:3617306/101‑1 (MQ=255)
                                                                              ggTCCGGCGGGGACGGAGGTCAGGG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTGGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCg                         <  2:381983/100‑1 (MQ=255)
                                                                              aGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCg                         >  1:1062906/1‑101 (MQ=255)
                                                                               ggccgggggggGCGGGGGGCCACGCCCCCCCGGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCg                         <  2:1258523/94‑1 (MQ=255)
                                                                                 cccGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCgggg                      >  1:4449458/1‑101 (MQ=255)
                                                                                  ccGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgt                     >  2:4834549/1‑101 (MQ=255)
                                                                                      ggggACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtcgtc                 >  1:2397780/1‑101 (MQ=255)
                                                                                        ggACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCt               >  2:4075673/1‑101 (MQ=255)
                                                                                        ggACGGGGGGCAGAC.CCCCCCTGCCCCCCGGGGGCCGAGCGCTCAGGCGTGTTGGGGGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCt               <  1:4381470/101‑1 (MQ=255)
                                                                                        gaaCGGGGGCCCGCA.CCCCCGGGCCCCCCTGGGGCCGGGCGCTGAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCt               <  2:370863/99‑1 (MQ=255)
                                                                                          gcGGGGGGCAGAG.CCCCCCTGCCCCCCGGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTg             <  1:2992892/100‑1 (MQ=255)
                                                                                          agggggggCAGAG.CCCCCCTGCCCCCCGGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTg             <  1:3394318/99‑1 (MQ=255)
                                                                                           cGGGGGGCAGAG.CCCCCCTGCCCCCCGGGGGCCGGGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTgg            <  2:2616961/101‑1 (MQ=255)
                                                                                            ggggggCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTGgc           >  2:3729546/1‑101 (MQ=255)
                                                                                             gggggCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTGgcg          >  2:1702228/1‑101 (MQ=255)
                                                                                                 gCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTGGCGCAGc      >  1:2033217/1‑101 (MQ=255)
                                                                                                  cAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCTAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTGGCGCAGCg     >  2:3224143/1‑101 (MQ=255)
                                                                                                  cAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTGGCGCAGCg     >  2:4346844/1‑101 (MQ=255)
                                                                                                  cAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTGGCGCAGCg     >  1:229975/1‑101 (MQ=255)
                                                                                                   agag.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTGGCGCAGCgg    >  1:378089/1‑101 (MQ=255)
                                                                                                   agag.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTGGCGCAGCgg    >  1:594941/1‑101 (MQ=255)
                                                                                                   agag.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTGGCGCAGCgg    >  2:893950/1‑101 (MQ=255)
                                                                                                    gag.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTGGCGCAGCGGa   >  2:3135657/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
CACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAG.CCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTCCTTGGCGCAGCGGAT  >  NC_014034/3004001‑3004201

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG