Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,068,163 A→T 2.9% intergenic (‑146/+146) glcD ← / ← RCAP_rcc02872 glycolate dehydrogenase, subunit GlcD/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,068,1630A→T 2.9% 4.1 ‑0.6 ‑0.2140intergenic (‑146/+146)glcD/RCAP_rcc02872glycolate dehydrogenase, subunit GlcD/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (97/38):  total (101/39)
Fisher's exact test strand distribution p-value = 5.75e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.11e-01

Only 100 of 197 total aligned reads displayed.

ATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGG  >  NC_014034/3068063‑3068262
                                                                                                        |                                                                                                       
aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCa                                                                                                         <  1:1429739/101‑1 (MQ=255)
  tCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAgc                                                                                                       <  2:4160680/101‑1 (MQ=255)
  tCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAgc                                                                                                       >  2:3665169/1‑101 (MQ=255)
   cgcgCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAgcg                                                                                                      >  1:438136/1‑101 (MQ=255)
   cgcgCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAgcg                                                                                                      >  2:791169/1‑101 (MQ=255)
    gcgcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAgcgc                                                                                                     >  2:1942028/1‑101 (MQ=255)
     cgcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGcgcc                                                                                                    >  1:3129191/1‑101 (MQ=255)
       cAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGcgcccg                                                                                                  >  1:111968/1‑101 (MQ=255)
          ccGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCgcccgccc                                                                                               >  1:4057091/1‑101 (MQ=255)
           cGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGGGGTCATGCGGAACAGTGGCCTCGGGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGcccgcccc                                                                                              <  2:845270/101‑1 (MQ=255)
           cGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGcccgcccc                                                                                              >  2:3799549/1‑101 (MQ=255)
           cGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGcccgcccc                                                                                              >  2:3826837/1‑101 (MQ=255)
           cGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGcccgcccc                                                                                              <  2:4569466/101‑1 (MQ=255)
            gAAACGATGGGCAGGCTGACAGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACAGCCGAGGG.CCCAAA.GGCC.CT.CGGCCACGGcaccccccg                                                                                             >  2:1660352/1‑101 (MQ=21)
             aaaCGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGcccgccccgc                                                                                            >  1:1807698/1‑101 (MQ=255)
              aaCGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGCGGCCTCGTGACCGCCGAGGG.CCCAAA.GTCC.CT.TGGCCAGCTCcccccccccc                                                                                           >  1:802132/1‑101 (MQ=21)
               aCGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCcgccccgccc                                                                                          <  1:2912370/101‑1 (MQ=255)
               aCGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCcgccccgccc                                                                                          >  2:4089727/1‑101 (MQ=255)
               aCGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCcgccccgccc                                                                                          <  1:599410/101‑1 (MQ=255)
                 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCAt                                                                                        <  2:2979522/101‑1 (MQ=255)
                 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCAt                                                                                        <  2:3509030/101‑1 (MQ=255)
                 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCAt                                                                                        <  2:3379692/101‑1 (MQ=255)
                  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATg                                                                                       >  2:1822147/1‑101 (MQ=255)
                   tGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCCTgg                                                                                      >  2:3717739/1‑101 (MQ=255)
                    gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGGGGAACAGTGGCCTCGTGCCCGCCGAGGG.CCCAAA.GGGC.CT.CGGCCGTCGCCCGCCCCGCCCAAggc                                                                                     >  1:2837124/1‑101 (MQ=18)
                    gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATggc                                                                                     >  2:2377115/1‑101 (MQ=255)
                      gCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATggcgg                                                                                   <  2:1533148/101‑1 (MQ=255)
                       cAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATggcggg                                                                                  <  2:3281764/101‑1 (MQ=255)
                           cTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCCCCGCGGGCG.Ct                                                                             >  1:974491/1‑101 (MQ=255)
                             gACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCCTGGCGGGGG.CCtc                                                                           >  1:2111807/1‑101 (MQ=255)
                             gACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGGG.CCtc                                                                           >  2:3781759/1‑101 (MQ=255)
                             gACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTtc                                                                           >  2:2015182/1‑101 (MQ=255)
                             gACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTtc                                                                           >  1:4813241/1‑101 (MQ=255)
                                cGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.cg                                                                       >  2:3777837/1‑101 (MQ=255)
                                cGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CCtcccc                                                                        >  2:697190/1‑98 (MQ=255)
                                   ggCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGGG.GCTCT.ccccc                                                                    >  1:1730512/1‑101 (MQ=18)
                                    gCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CCTCT.cgcccc                                                                   >  1:2396462/1‑100 (MQ=255)
                                       aTCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCcccccg                                                                >  2:969385/1‑101 (MQ=255)
                                       aTCGCCGAGGCCATGCGGACCAGGGGCCTCGTGCCCGCCGAGGG.CCAAAA.GCCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCccgccg                                                                <  1:2577653/101‑1 (MQ=17)
                                          gCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCgggg                                                             >  2:3321377/1‑101 (MQ=255)
                                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGgggcg                                                           <  1:4484842/101‑1 (MQ=255)
                                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCCCCCgggcg                                                           >  2:3983163/1‑101 (MQ=255)
                                             gAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCCgggcgg                                                          >  2:2880350/1‑101 (MQ=255)
                                              aGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGgggcggg                                                         >  1:292373/1‑101 (MQ=255)
                                              aGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CCCCCGCCGgggcggg                                                         >  1:3359564/1‑101 (MQ=255)
                                               ggTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCGGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGgggcgggc                                                        <  1:2702887/101‑1 (MQ=255)
                                                 tCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGgcgc                                                      <  2:650604/101‑1 (MQ=255)
                                                  caggCGGAACAGTGCCCTCGTGCCCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGcc                                                     <  1:3890401/98‑1 (MQ=255)
                                                  cATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGcc                                                     <  1:2541991/101‑1 (MQ=255)
                                                   aTGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCg                                                    <  1:3596719/101‑1 (MQ=255)
                                                     gCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCCCCGGGGGGGGCGCCGGc                                                  >  2:141664/1‑101 (MQ=255)
                                                      cgaaaCAGTGGTCCCATGACCCCCGAGTG.CCCCAA.GGCA.CT.CGGGCAGCGCCCGCCCCGCCCATGGCGGGNG.CTTCTCCCCCCCCCGgggcggggggggcc                                                  >  1:614929/4‑94 (MQ=18)
                                                         accAGTGGCCTGGTGCCCGCGGGGGG.CCAAAG.GCCC.CG.CGGCCGGCGCCCGCCCCGCCCAGGGCGGGGG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCctc.t                                             <  2:3267444/99‑1 (MQ=11)
                                                          acgggggCCTCGGGCCCGCGGAGGC.CCCAAG.GCCC.CT.CGGCCGGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCTc.tg                                            <  1:2173345/96‑1 (MQ=11)
                                                            gggggCCCCGTGCCCGCCGAGGC.CCCAAG.GGCC.CT.CGGCCAGCCCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCTc.tgct                                          <  2:248228/98‑1 (MQ=14)
                                                            aGTGGCCTGGGGACCGCCG.GGG.CCCAAACGGCC.GC.CGGCCTGCCCCCGCCCCGCCCATGGCGGGCG.CTTGT.CGCCCGCCGGGGCGGGCGCCGGCCTc.tgct                                          <  1:3226826/101‑1 (MQ=11)
                                                            aGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGGGCCGGCCTc.tgcc                                          >  2:3676811/1‑100 (MQ=255)
                                                              tGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCCC.tgctgc                                        >  2:4821916/1‑101 (MQ=255)
                                                              tGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCCCCGGGGCCGGGGCCCGCCCC.tgctgc                                        >  1:4914630/1‑101 (MQ=14)
                                                               ggCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCCC.CGCTgcg                                       >  1:3897088/1‑101 (MQ=255)
                                                                gCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCCCCGGGGCGGGGGCCGGCCCC.TGCTgcgc                                      >  1:1779717/1‑101 (MQ=255)
                                                                 ccTCGTGACCGCGGAGGG.CCCAAG.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGcc                                     <  2:3087477/101‑1 (MQ=255)
                                                                   tCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCCGGGCGGGGGCCCGCCCC.CGCTGCCCCT.g                                  >  1:2226333/1‑101 (MQ=14)
                                                                    cctGACCGCCGGGGGCCCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGGG.CTTTTGCGCCGCCCGGGGCGGGGGCCGGGCCC.TCCGGCGCCt                                    >  2:2168344/3‑101 (MQ=2)
                                                                    cGTGCCCGCCGGGGG.CCCAAA.GCCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.gg                                 <  2:105684/101‑1 (MQ=255)
                                                                      gccccgccgAGGC.CCCAAG.GCCC.CT.CGGCCAGCCCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.gggg                               <  1:4090030/98‑1 (MQ=14)
                                                                         ccccgggggg.CCCAAG.GCCC.CT.GGCCCGGCGCCCGCCCCGCCGATGGGGGGCG.CTTCT.CCCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGaaa                            <  1:3859189/94‑1 (MQ=17)
                                                                         cccccgAGGC.CCCAAA.GGCC.CC.CGCCCGCCCCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGGGGGCGCCGGCCTC.TGCTGCGCCT.GGGGaaa                            <  2:4350814/98‑1 (MQ=17)
                                                                          cgccgGGGC.CCAAAA.GCCC.CC.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCC.CGCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGAAAc                           <  1:178247/101‑1 (MQ=14)
                                                                          cgccgAGGG.CCAAAA.GCCC.CT.CGGCCGGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCGGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGAAAc                           <  2:438071/101‑1 (MQ=17)
                                                                          ccccgGGGC.CCCAAG.GCCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGAAAc                           <  1:4865169/99‑1 (MQ=14)
                                                                          ccccgGGGC.CCCAAG.GCCC.CT.CGCCCCGCCCCCCCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGAAAc                           <  1:1265759/99‑1 (MQ=14)
                                                                            gccgggg.CCCAAACGCCC.CT.CGGCCGGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGAAACg                          <  1:2067600/97‑1 (MQ=17)
                                                                            ccgAGGG.CCCAAA.GGCC.CT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCCTTCG.CGCCCCCCGGGCTGGGCGCAGGGCTC.TGCCACACGT.GGGGacggg                          >  1:3375970/1‑97 (MQ=14)
                                                                             cgAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGGGCCGGCCTC.TGCTGCGCCC.GGGGCAACGGt                        >  1:1113180/1‑101 (MQ=255)
                                                                               aGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCCC.TGCTGCGCCT.CGGGGAACGGtgt                      >  2:3298983/1‑99 (MQ=255)
                                                                                ggg.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGGGCCGGCCCC.TGCTGCGCCT.GGGGAAACGGGCtt                     >  1:2445098/1‑101 (MQ=255)
                                                                                ggg.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCCGGGGGGGGGCCGGCCCC.CCCTGCGCCC.GGGGGAACCGGCtt                     >  2:3543371/1‑101 (MQ=14)
                                                                                  g.CCCAAG.GCCC.CC.CGGCGGGCCCCCCCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGAAACGGTCTTgg                   <  2:448316/101‑1 (MQ=17)
                                                                                  g.CCCAAA.GGCCGCC.CGGCCAGCGCCCGCCCCGCCCCTGGCGGGCG.CTTCT.CGCCCGCCGGGCCGGGCGCCGGCCTC.TGTTGTGCCTCGCGGAAACGGTCtt                     <  2:4725796/101‑1 (MQ=11)
                                                                                  g.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGAAACGGGCTTgg                   >  1:4234290/1‑101 (MQ=255)
                                                                                   ggccAAA.CGCC.CTCCGCCCAGCGCCCGCCCCGCCCGTGGGGGGCG.CTTCT.CGCCCGCGGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGAAACGGTCTTg                    <  2:2099481/99‑1 (MQ=17)
                                                                                    cccAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGGGCCGGCCTC.TGCCGCGCCC.CGGGAAACCGGCTTGGc                  >  2:1433028/1‑101 (MQ=14)
                                                                                     gctgcagccc.cG.CGGCCGGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCTGGCGCCGGCCTC.TGGTGTGCTT.GGGGATGCGGTCGTgct                  <  1:2623582/93‑3 (MQ=17)
                                                                                      caaagggCC.CT.CGGCCGGCCCCCGCCCCGCCCATGGGGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGAAACGGTCTTGGCa                 <  2:3625302/96‑1 (MQ=18)
                                                                                      cAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGGGCCGGCCTC.TGCTGCGCCT.GGGGGAACGGTCTTGGCAc                >  1:3180409/1‑101 (MQ=255)
                                                                                       aaa.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGGGCCGGCCTC.CGCTGCGCCC.GGGGGAACGGGCTTGGCAcc               >  1:2559885/1‑101 (MQ=17)
                                                                                        aagggCC.CG.CGGCAAGCGCCCCCCCCGCCCAGGGCGGGCG.CTTCT.CGCCCGCGGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGAAACGGTCTTGGCAcc               <  1:2938053/98‑1 (MQ=18)
                                                                                        aagggCC.CC.CGGCCGGCCCCCCCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGAAACGGTCTTGGCAcc               <  2:2893305/98‑1 (MQ=18)
                                                                                        aaggccc.cT.CGGCAGGCGCCCGCCCCGCCCAGGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGAAACGGTCTTGGCAcc               <  2:4896822/96‑1 (MQ=18)
                                                                                        aa.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGGGGGGGCCGGCCCCTTGCCG.GCCT.GGGGGAACGGTCCTGGCAccc              >  2:2411259/1‑100 (MQ=14)
                                                                                         a.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGGAACGGGCTTGGCACCGc             >  2:1249362/1‑101 (MQ=255)
                                                                                           ggCC.CC.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGGGGGCGCCGGCCTC.TGCTGCGCCT.GGGGAAACGGTCTTGGCACCGcc            <  2:2960883/101‑1 (MQ=255)
                                                                                            gCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGGAACGGTCTTGGCACCGccc           >  1:3770067/1‑101 (MQ=255)
                                                                                            gCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCCGCCCC.CGCGGCGCCC.GGGGGAACGGGCTTGGCCCCGccc           >  1:46698/1‑101 (MQ=17)
                                                                                              ccccggggCCGGCCCCCGCCCCGCCCATGGGGGGCG.CTTCC.CGCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGAAACGGTCTTGGCACCGccc           <  1:1942165/95‑1 (MQ=18)
                                                                                              c.cT.CGCCCGGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCgg         <  2:111952/101‑1 (MQ=255)
                                                                                                   gggCCAGCCCCCGCCCCGCCCATGGCGGGCT.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCt      <  2:46513/100‑1 (MQ=255)
                                                                                                   cGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGAAACGGGCTTGGCACCGCCCGGGcc      >  2:2434724/1‑100 (MQ=255)
                                                                                                     gCCCGCGCCCGCCCCCCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTcc    <  1:2282411/101‑1 (MQ=255)
                                                                                                        |                                                                                                       
ATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGG.CCCAAA.GGCC.CT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCG.CTTCT.CGCCCGCCGGGGCGGGCGCCGGCCTC.TGCTGCGCCT.GGGGAAACGGTCTTGGCACCGCCCGGGCTCCGG  >  NC_014034/3068063‑3068262

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG