Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,342,566	C→G	100%	R154P (CGG→CCG)	RCAP_rcc03145 ←	AsnC/Lrp family transcriptional regulator

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,342,566	0	C→G	100.0%	48.0	13	R154P (CGG→CCG)	RCAP_rcc03145	AsnC/Lrp family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (0/12):  ref base (0/0):  total (0/13)

GTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACACCCAGCACCGGGGCAGCCGCGGCCGCGGGTTGCGGCAGGACAGCT  >  NC_014034/3342468‑3342593                                                                                                   |                            gTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggc                           <  1:328846/101‑1 (MQ=255) gTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggc                           <  1:3960110/101‑1 (MQ=255)  tctcGGGGCTGATCTCGTTCAGCGGGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcg                          <  2:2354019/101‑1 (MQ=255)   ctcGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcgg                         <  2:883257/101‑1 (MQ=255)     cGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggcc                       <  2:3909750/101‑1 (MQ=255)     cGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggcc                       <  2:3141075/101‑1 (MQ=255)     cGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCgcggcggcc                       <  1:4857882/101‑1 (MQ=255)            gATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGtt                <  2:2627586/101‑1 (MQ=255)            gATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGtt                <  1:1954557/101‑1 (MQ=255)            gATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGtt                <  1:1300514/101‑1 (MQ=255)             aTCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTg               <  2:1034780/101‑1 (MQ=255)             aTCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTg               <  2:3100613/101‑1 (MQ=255)              tctcGTTCAGCGGGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGc              <  2:1340640/101‑1 (MQ=255)              tctcGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGTCACCAGCGCGGGCTTGCGGACGACGCCCGGCACCGGGGCCGCTGCGGCCGCGGGtttc              <  2:3931497/101‑3 (MQ=255)              tctcGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGc              <  2:1085781/101‑1 (MQ=255)              tctcGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGc              <  2:1445337/101‑1 (MQ=255)                 cGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGc           <  1:2691206/101‑1 (MQ=255)                      aGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcac      <  1:2658778/101‑6 (MQ=255)                       gCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcacc     <  2:1242887/101‑7 (MQ=255)                          aGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACGCCCGGCACCGGGGCCGCGGCGGCCGCGGGTTGCGGcggcaccggc  <  2:1865585/101‑10 (MQ=255)                                                                                                   |                            GTCTCGGGGCTGATCTCGTTCAGCGAGGGTTGCGGCGGCAGCGGCTGGCCCGCGGGCACCAGCGCGGGCTTGCGCACGACACCCAGCACCGGGGCAGCCGCGGCCGCGGGTTGCGGCAGGACAGCT  >  NC_014034/3342468‑3342593 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 38 ≤  ATCG