BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,539,498	T→G	100%	D261E (GAT→GAG)	prpD →	2‑methylcitrate dehydratase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,539,498	0	T→G	100.0%	1066.6	286	D261E (GAT→GAG)	prpD	2‑methylcitrate dehydratase
Reads supporting (aligned to +/- strand): new base (136/150): ref base (0/0): total (136/150)

Only 100 of 320 total aligned reads displayed.


ATCGAGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGATGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAGG  >  NC_014034/3539400‑3539595


aTCGAGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGgc                                                                                                 <  2:2446521/101‑1 (MQ=255)


  cGAGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGgcgc                                                                                               >  2:4106012/1‑101 (MQ=255)


   gAGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGcgcg                                                                                              >  2:734020/1‑101 (MQ=255)


     ggCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGtt                                                                                            <  2:397160/101‑1 (MQ=255)


      gCGGTGAACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTa                                                                                           <  2:5049423/101‑1 (MQ=255)


       cGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTac                                                                                          <  1:4800489/101‑1 (MQ=255)


       cGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTac                                                                                          >  2:112862/1‑101 (MQ=255)


       cGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTac                                                                                          <  2:4461631/101‑1 (MQ=255)


         gTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTacac                                                                                        <  2:3698141/101‑1 (MQ=255)


          tGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACAcc                                                                                       >  1:2484855/1‑101 (MQ=255)


          tGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACAcc                                                                                       <  1:4636439/101‑1 (MQ=255)


           ggACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGTCGCGTTACACCg                                                                                      >  1:1175216/1‑101 (MQ=255)


           ggACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCg                                                                                      >  1:3901548/1‑101 (MQ=255)


           ggACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCg                                                                                      >  1:4110958/1‑101 (MQ=255)


           ggACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCg                                                                                      <  2:307473/101‑1 (MQ=255)


            gACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGt                                                                                     >  1:3940018/1‑101 (MQ=255)


            gACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGt                                                                                     >  1:1397661/1‑101 (MQ=255)


              ccGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTcc                                                                                   <  1:1585780/101‑1 (MQ=255)


              ccGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTcc                                                                                   <  1:2105817/101‑1 (MQ=255)


              ccGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTcc                                                                                   >  2:4891621/1‑101 (MQ=255)


                   cccTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTg                                                                              <  2:1341501/101‑1 (MQ=255)


                     cTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGcc                                                                            >  2:693094/1‑101 (MQ=255)


                      tGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGccc                                                                           <  1:101698/101‑1 (MQ=255)


                       gcgcGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCg                                                                          >  2:1785496/1‑101 (MQ=255)


                       gcgcGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCg                                                                          <  1:1998913/101‑1 (MQ=255)


                        cgcgGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCgg                                                                         <  1:99905/101‑1 (MQ=255)


                          cggcgAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGcc                                                                       <  1:3391703/101‑1 (MQ=255)


                            gcgAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCAt                                                                     >  1:1510978/1‑101 (MQ=255)


                             cgAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATg                                                                    >  2:4821928/1‑101 (MQ=255)


                              tcgccccccaccAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATgg                                                                   >  2:1489801/11‑101 (MQ=255)


                              gAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATgg                                                                   <  1:4548052/101‑1 (MQ=255)


                              gAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATgg                                                                   <  1:2561927/101‑1 (MQ=255)


                               aaGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGc                                                                  <  1:822481/101‑1 (MQ=255)


                                    gcCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGcc                                                             >  1:2256030/1‑101 (MQ=255)


                                       cggatccccTCCGGGGAGGGGGAACACGGTTTCATCGCCTGGAGGCTCTCGGGCCCAGAGGCGCTTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGaa                                                          <  2:1604993/96‑1 (MQ=255)


                                        ccAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGaaa                                                         >  2:4530205/1‑101 (MQ=255)


                                         cAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGCGCCGAAAc                                                        >  1:3886901/1‑101 (MQ=255)


                                         cAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAAc                                                        >  1:237001/1‑101 (MQ=255)


                                         cAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAAc                                                        <  2:4624420/101‑1 (MQ=255)


                                         cAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAAc                                                        <  1:4835031/101‑1 (MQ=255)


                                          aGCCCCGCCTGGGAGGGTGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAAcg                                                       >  1:4912000/1‑101 (MQ=255)


                                            ccccGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAAcgcg                                                     >  2:2977212/1‑101 (MQ=255)


                                               cGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCa                                                  <  2:3615591/101‑1 (MQ=255)


                                                gCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCAt                                                 >  2:1865525/1‑101 (MQ=255)


                                                 ccTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATc                                                <  1:4282545/101‑1 (MQ=255)


                                                   tGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCt                                              <  1:3573563/101‑1 (MQ=255)


                                                    gggAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCtt                                             <  1:1323732/101‑1 (MQ=255)


                                                    gggAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCtt                                             >  1:4092034/1‑101 (MQ=255)


                                                    gggAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCtt                                             <  2:2298563/101‑1 (MQ=255)


                                                    gggAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCtt                                             <  2:2389047/101‑1 (MQ=255)


                                                     ggAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTg                                            <  2:3625746/101‑1 (MQ=255)


                                                     ggAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTg                                            <  1:264986/101‑1 (MQ=255)


                                                       aGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTCCCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGac                                          <  2:3901256/101‑1 (MQ=255)


                                                       aGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGac                                          <  1:445751/101‑1 (MQ=255)


                                                        gggggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGaca                                         <  2:2156342/101‑1 (MQ=255)


                                                        gggggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGaca                                         <  1:4058383/101‑1 (MQ=255)


                                                        gggggAAGACGGTTTCACCGCCTGGATGCTCTCGGGCCCAGAGACGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGaca                                         <  2:2832933/101‑1 (MQ=255)


                                                        gggagAAGTCGGTTTCATCGCCTGGATGCTCTCGGGCCTGGAGGCGCGTTACACCGCCCCCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGaca                                         <  1:1604053/101‑1 (MQ=255)


                                                         ggggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGacac                                        <  2:597484/101‑1 (MQ=255)


                                                          gggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACAcc                                       >  2:3160024/1‑101 (MQ=255)


                                                            gAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCta                                     >  2:2705899/1‑101 (MQ=255)


                                                             aaGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCtat                                    <  2:2581656/101‑1 (MQ=255)


                                                             aaGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCtat                                    >  1:400912/1‑101 (MQ=255)


                                                             aaGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCtat                                    <  1:4418810/101‑1 (MQ=255)


                                                              aGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCtata                                   <  1:3757947/101‑1 (MQ=255)


                                                               ggcGGTTTCAGCCCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATAc                                  <  2:5015464/99‑1 (MQ=255)


                                                               gACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATAc                                  <  1:4651860/101‑1 (MQ=255)


                                                                gcGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATAcc                                 <  2:1212988/100‑1 (MQ=255)


                                                                 cGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCa                                <  1:2944369/101‑1 (MQ=255)


                                                                  ggTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCaa                               <  1:2795491/101‑1 (MQ=255)


                                                                   gTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAg                              >  1:3074167/1‑101 (MQ=255)


                                                                   gTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAg                              >  1:3478236/1‑101 (MQ=255)


                                                                   gTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAg                              >  1:634368/1‑101 (MQ=255)


                                                                      tCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGaa                           <  2:4738170/101‑1 (MQ=255)


                                                                      tCATCGCCTGGATGCTCTCGGCCCGAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGaa                           <  2:4917013/101‑1 (MQ=255)


                                                                       cATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAat                          <  1:168177/101‑1 (MQ=255)


                                                                        aTCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAata                         >  2:141550/1‑101 (MQ=255)


                                                                         tCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAatat                        <  1:112878/101‑1 (MQ=255)


                                                                          cGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATAtt                       >  1:5028671/1‑101 (MQ=255)


                                                                            ccTGGATGCTCTCGGGCCCAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTcg                     <  1:1785629/101‑1 (MQ=255)


                                                                             cTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTcgg                    >  2:1681261/1‑101 (MQ=255)


                                                                              tGGATGCTCTCGGGCCCAGAGGCGCTTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTcggc                   <  2:2040103/101‑1 (MQ=255)


                                                                              tGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTcggc                   >  1:4958845/1‑101 (MQ=255)


                                                                                gATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTcggcgg                 >  1:419021/1‑101 (MQ=255)


                                                                                 aTGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGa                >  1:3242203/1‑101 (MQ=255)


                                                                                    ctctCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAata             >  2:4256247/1‑101 (MQ=255)


                                                                                     tctcGGGCCCAGAGGCGCGTTCCCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAatat            <  2:4418445/101‑1 (MQ=255)


                                                                                      ctcGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATc           >  1:3021954/1‑101 (MQ=255)


                                                                                      ctcGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATc           >  2:4676686/1‑101 (MQ=255)


                                                                                        cGGGCCCAGAGGCTCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCaa         <  2:3827047/101‑1 (MQ=255)


                                                                                        cGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCaa         <  1:3160275/101‑1 (MQ=255)


                                                                                        cGGGCCCAGAGGCGCGCCCCCCCGCCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCaa         <  2:2242735/101‑1 (MQ=255)


                                                                                        cGGGCCCAGAGGCCCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCaa         <  1:2768051/101‑1 (MQ=255)


                                                                                         gggCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCaaa        >  2:763180/1‑101 (MQ=255)


                                                                                          ggCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGCATATTCGGCGGAATATCAAAg       <  1:3721272/101‑1 (MQ=255)


                                                                                            cccAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGcc     >  1:2312398/1‑101 (MQ=255)


                                                                                             ccAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCa    >  1:69360/1‑101 (MQ=255)


                                                                                             ccAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCa    >  2:651906/1‑101 (MQ=255)


                                                                                               aGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAgg  <  1:3676491/101‑1 (MQ=255)


                                                                                               aGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAgg  <  1:4985867/101‑1 (MQ=255)


ATCGAGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGATGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAGG  >  NC_014034/3539400‑3539595

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG