Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 398,935 C→T 100% E381K (GAA→AAA)  RCAP_rcc00372 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034398,9350C→T100.0% 989.3 253E381K (GAA→AAA) RCAP_rcc00372hypothetical protein
Reads supporting (aligned to +/- strand):  new base (129/124):  ref base (0/0):  total (129/124)

Only 100 of 258 total aligned reads displayed.

TGTGGAACCGGTCCTGTCCCC.GCGGTTGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTCCGGCGCGGTCGAATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGATCAACACGGTATCTTGCCAGAAC  >  NC_014034/398837‑399032
                                                                                                   |                                                                                                 
 gtgGAACCGGTCCTGTCCCC.GCGGTTGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCGCGCCGTTTTCgg                                                                                                >  1:2014360/1‑101 (MQ=255)
     aaCCGGTCCTGTCCCC.GCGGTTGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGcgcg                                                                                            >  1:3079746/1‑101 (MQ=255)
      aCCGGTCCTGTCCCC.GCGGTTGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCgg                                                                                           >  2:1864466/1‑101 (MQ=255)
       ccGGTCC.GTCCCCGGCGGTTGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGt                                                                                          <  2:2286114/101‑1 (MQ=25)
        cGGTCCTGTCCCC.GCGGTTGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTc                                                                                         <  1:2271635/101‑1 (MQ=255)
        cGGTCCTGTCCCC.GCGGTTGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTc                                                                                         <  2:728771/101‑1 (MQ=255)
        cGGTCCGGCCCCC.GCGGTTGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTCGTCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTc                                                                                         <  2:4368541/101‑1 (MQ=37)
         ggTCCTGTCCCC.GCGGTTGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCg                                                                                        >  1:309122/1‑101 (MQ=255)
         ggTCCTGTCCCC.GCGGTTGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCg                                                                                        >  2:3998710/1‑101 (MQ=255)
           tctggccgcc.GCGTTTCCCCCGGCGGGGGGGAAAAAGCGTCGCTTTTCCTCACCCGCCCGAACCGAGGTCTGAGCCCCCCGCCGTTTTCGGCGCGGTCgga                                                                                      <  2:3033945/93‑3 (MQ=255)
            ccTGTCCCC.GCGGTTGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGAt                                                                                     <  2:1342650/101‑1 (MQ=255)
              tgccccc.GCGGTTGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGc                                                                                   <  1:272483/98‑1 (MQ=255)
              tGTCCCG.GCGGTTGCCCGGGGGGGGGGAAAAAAGCGGTGCTTTTCTTGATCGGCCCGAACCGAGGTTTCAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGc                                                                                   <  2:1891998/101‑1 (MQ=255)
              tGTCCCC.GCGGTTGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGc                                                                                   >  1:4055261/1‑101 (MQ=255)
              tGTCCCC.GCGGTTGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCCCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGc                                                                                   <  1:3831146/101‑1 (MQ=255)
                tCCCC.GCGGTTGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCgg                                                                                 <  1:3229490/101‑1 (MQ=255)
                 cccc.GCGGTTGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGc                                                                                >  2:647050/1‑101 (MQ=255)
                 cccc.GCGGTTGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGc                                                                                >  2:2395987/1‑101 (MQ=255)
                   cc.GCGGTTGCCCCGGCGGGCGGGAAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCtt                                                                              <  2:4782022/101‑1 (MQ=255)
                    c.gcgTTTGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTg                                                                             <  2:1041981/101‑1 (MQ=255)
                      ggggTTGCCCGGGCGGGCGGGGAAAGGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGa                                                                            <  2:3240221/99‑1 (MQ=25)
                        ggTTGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTCATg                                                                          >  2:1243879/1‑101 (MQ=255)
                          gtGCCCCGGCGGTGGGGAAAACGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTa                                                                        <  2:4865494/100‑1 (MQ=37)
                           tGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTAt                                                                       <  1:4189861/101‑1 (MQ=255)
                           tGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGaatgat                                                                       >  2:4719681/1‑96 (MQ=255)
                           tGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTAt                                                                       >  1:112329/1‑101 (MQ=255)
                           tGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTAt                                                                       >  2:3511319/1‑101 (MQ=255)
                            gCCCGGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTAtt                                                                      <  2:348833/101‑1 (MQ=255)
                             ccccGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTc                                                                     <  1:1643348/101‑1 (MQ=255)
                              ccgggcgggcgGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTcg                                                                    <  1:1178628/98‑1 (MQ=37)
                              cccGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGGAtttg                                                                    >  1:2858513/1‑99 (MQ=255)
                                   ggggcgggAAAAAGCGTTGCTTTTCCTGACCCGCCCGAACCGAGGTCTCAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCAc                                                               <  2:912182/100‑1 (MQ=255)
                                   cgggcgggGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTTgcgccc                                                               >  1:4931053/1‑99 (MQ=255)
                                   cgggcgggGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCAc                                                               >  1:4588381/1‑101 (MQ=255)
                                     ggcgggGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCAcgg                                                             <  2:630483/101‑1 (MQ=255)
                                     ggcgggGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCAcgg                                                             >  1:1762688/1‑101 (MQ=255)
                                       cgggGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGGAcggcg                                                           >  2:4921148/1‑101 (MQ=255)
                                       cgggGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCAcggcg                                                           >  2:2606379/1‑101 (MQ=255)
                                         ggaaaaaGCGGTGCTTTTCCTATTCCGCCAAAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACggcggc                                                         <  2:2024981/98‑1 (MQ=255)
                                            gaacgCGGTGCTTTCCCTGGCCCGCCGGAACCGAGGGCTCCGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCaaa                                                      <  1:1329465/97‑1 (MQ=255)
                                            aaaaGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCaaa                                                      <  1:712478/101‑1 (MQ=255)
                                            aaaaGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCaaa                                                      >  1:3545600/1‑101 (MQ=255)
                                            aaaaGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCaaa                                                      >  2:1441005/1‑101 (MQ=255)
                                            aaaaGCGGTGCTTTTCCTGACCCGCCCAAACCGAGGTCTCAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCaaa                                                      <  2:3443193/101‑1 (MQ=255)
                                             aaaGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAg                                                     >  2:5053198/1‑101 (MQ=255)
                                             aaaGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGGATTCGCGCACCGCGGCCAAg                                                     >  1:3895455/1‑101 (MQ=38)
                                              aaGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTAAGACCCCCGCCGTTTTCGGCGCGGGCGGGTGGGGCTTGATGAATTATCGGACCGCGGCgaacg                                                    >  1:3497221/1‑99 (MQ=19)
                                                  ggTTCTTTTCCTGACCCGCCCGAACCGAGTTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCa                                                <  2:3214674/101‑1 (MQ=255)
                                                  ggTGTTTTTCCTGTTCCACCCGAACCGAGGTCCCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCa                                                <  2:2408045/101‑1 (MQ=255)
                                                   gTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCAt                                               <  1:1996298/101‑1 (MQ=255)
                                                   gTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCAt                                               <  1:1482145/101‑1 (MQ=255)
                                                   gTGCTTTTCCGGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAGAGGCCCAt                                               >  1:4362274/1‑101 (MQ=255)
                                                    tGCTTTTCCTGATCCGCCCGAACCGGGGTCTCAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCAtt                                              <  2:3263427/101‑1 (MQ=255)
                                                    tGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCAtt                                              <  2:2107386/101‑1 (MQ=255)
                                                    tGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCAtt                                              >  1:579691/1‑101 (MQ=255)
                                                     gCTTTTCCTGATCCGCCCGAACCGAGGGCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCCCGGTGGCAGAGGGCCACTg                                             >  2:4806617/1‑101 (MQ=255)
                                                      cTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGc                                            >  1:1381529/1‑101 (MQ=255)
                                                       ttttCCTGACCCGCCCGAACCGAGGTCTCAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCa                                           <  2:1700616/101‑1 (MQ=255)
                                                       tttcCCTGGTCCGCCCGACCCGAGCTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCa                                           <  2:2303256/101‑1 (MQ=255)
                                                        tttCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGGAAAGGCCCCTTGCAc                                          >  2:4109291/1‑101 (MQ=255)
                                                        tttCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCAc                                          >  1:649264/1‑101 (MQ=255)
                                                         ttCCTGACCCGCCCGACCCGAGGTCTCAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACg                                         <  2:3545330/101‑1 (MQ=255)
                                                          tCCTGACCCGCCCGAACCGAGGTCTCAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACgg                                        <  1:4523910/101‑1 (MQ=255)
                                                           ccTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAAGCCCATTTCCCGGt                                       >  2:1280472/1‑101 (MQ=255)
                                                              gacccGCCCGACCCGAGGTCTCAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAg                                    <  1:1811560/98‑1 (MQ=255)
                                                              gATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAg                                    >  2:2488811/1‑101 (MQ=255)
                                                              gATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGGCAg                                    >  2:1597196/1‑101 (MQ=255)
                                                               acccGCCCGACCCGAGGTCTCAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAgc                                   <  2:4015177/99‑1 (MQ=255)
                                                                    cccGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCCTTGCACGGTCAgcccgcc                              >  2:2664631/1‑101 (MQ=255)
                                                                    cccGAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAgcccccc                              >  1:1507854/1‑101 (MQ=255)
                                                                     ccGAACCGAGGTCTCAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAgcccgccc                             <  1:1597328/101‑1 (MQ=255)
                                                                       gAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAg                           >  1:4712777/1‑101 (MQ=255)
                                                                       gAACCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAg                           >  2:1472427/1‑101 (MQ=255)
                                                                        aaCCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCCgg                          >  2:309357/1‑101 (MQ=255)
                                                                        aaCCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAgg                          >  1:4447684/1‑101 (MQ=255)
                                                                         aCCGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCCGGa                         >  1:2602041/1‑101 (MQ=255)
                                                                          ccGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCCCGGTCAGCCCGCCCACGAt                        >  1:4847169/1‑101 (MQ=255)
                                                                          ccGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAgggt                        >  1:1256949/1‑99 (MQ=255)
                                                                          ccGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGAt                        >  2:2966510/1‑101 (MQ=255)
                                                                          ccGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGAt                        >  1:4143648/1‑101 (MQ=255)
                                                                          ccGAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGAt                        >  2:1729150/1‑101 (MQ=255)
                                                                          ccGAGGTCCCAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGAt                        <  1:4990159/101‑1 (MQ=255)
                                                                            gAGGTCTCAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGATCa                      <  1:1001311/101‑1 (MQ=255)
                                                                            gAGGTCTCAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGATCa                      <  2:1912770/101‑1 (MQ=255)
                                                                            gAGGTCTCAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGATCa                      <  1:3999012/101‑1 (MQ=255)
                                                                               gTCTCAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGATCAaca                   <  2:773604/101‑1 (MQ=255)
                                                                                tctcAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGATCAacac                  >  1:4949130/1‑101 (MQ=255)
                                                                                tctcAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGATCAacac                  <  2:309069/101‑1 (MQ=255)
                                                                                  tcAGACCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGATCAACACgg                >  1:2900736/1‑101 (MQ=255)
                                                                                   cAGCCCCCCGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGATCAACACGGt               <  1:2396151/101‑1 (MQ=255)
                                                                                       cccccGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGATCAACACGGTATCt           <  1:4762971/101‑1 (MQ=255)
                                                                                        ccccGCCGTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGATCAGCACGGTATCtt          <  2:3602121/101‑1 (MQ=255)
                                                                                          ccgccgTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGATCAACACGGTATCTTGc        <  2:1158759/101‑1 (MQ=255)
                                                                                          ccgccgTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGATCAACACGGTATCTTGc        >  1:53261/1‑101 (MQ=255)
                                                                                           cgccgTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGATCAACACGGTATCTTGcc       <  2:4447365/101‑1 (MQ=255)
                                                                                           cgccgTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGATCAACACGGTATCTTGcc       <  1:1896274/101‑1 (MQ=255)
                                                                                            gccgTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGATCAACACGGTATCTTGCCa      >  1:2129749/1‑101 (MQ=255)
                                                                                             ccgTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCCCGGTCAGCCCACCCAGGATCAACACGGTATCTTGCCAg     >  1:1975551/1‑101 (MQ=255)
                                                                                             ccgTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGATCAACACGGTATCTTGCCAg     <  1:3937873/101‑1 (MQ=255)
                                                                                              cgTTTTCGGCGCGGTCGGATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGATCAACACGGTATCTTGCCAGa    <  2:4058385/101‑1 (MQ=255)
                                                                                                   |                                                                                                 
TGTGGAACCGGTCCTGTCCCC.GCGGTTGCCCCGGCGGGCGGGGAAAAGCGGTGCTTTTCCTGATCCGCCCGAACCGAGGTCTCAGACCCCCGCCGTTTCCGGCGCGGTCGAATGCGGCTTGATGTATTCGCGCACGGCGGCAAAGGCCCATTGCACGGTCAGCCCGCCCAGGATCAACACGGTATCTTGCCAGAAC  >  NC_014034/398837‑399032

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG