Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 66,387 T→G 100% S518S (TCT→TCG flhA → flagellar biosynthesis protein FlhA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_01403466,3870T→G100.0% 801.9 217S518S (TCT→TCGflhAflagellar biosynthesis protein FlhA
Reads supporting (aligned to +/- strand):  new base (118/99):  ref base (0/0):  total (118/99)

Only 100 of 226 total aligned reads displayed.

GTGATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGACGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCTAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGATCC  >  NC_014034/66289‑66484
                                                                                                   |                                                                                                 
gTGATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGaa                                                                                                 >  2:4685015/1‑101 (MQ=255)
 tGATCCGGCGTAACTTCGGCCGTCTGATGACGCGCCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAc                                                                                                <  1:4333585/101‑1 (MQ=255)
 tGATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAc                                                                                                <  2:54694/101‑1 (MQ=255)
 tGATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAc                                                                                                >  1:790747/1‑101 (MQ=255)
 tGATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAc                                                                                                <  2:1760282/101‑1 (MQ=255)
  gATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAcc                                                                                               >  1:2624236/1‑101 (MQ=255)
   aTCCGGCGCACCTTGGCCCGTCTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAccg                                                                                              <  2:1474924/101‑1 (MQ=255)
    tCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAccgc                                                                                             <  1:389426/101‑1 (MQ=255)
     ccGTCGCAGCTTCGGCCGTCTGAGGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCTATCCCGCCCGCGCCGAGTCGAAccgcc                                                                                            <  1:1738910/101‑1 (MQ=255)
     ccGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAccgcc                                                                                            >  1:1400046/1‑101 (MQ=255)
       ggCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACcgccgc                                                                                          >  2:2652453/1‑101 (MQ=255)
         cgcAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCt                                                                                        <  2:292270/101‑1 (MQ=255)
         cgcAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCt                                                                                        >  2:1187623/1‑101 (MQ=255)
          gcAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGCCGAACCGCCGCCTg                                                                                       >  2:3327669/1‑101 (MQ=255)
           cAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGc                                                                                      >  1:4044000/1‑101 (MQ=255)
              cTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTg                                                                                   <  1:2964034/101‑1 (MQ=255)
                tCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGAc                                                                                 <  1:1935180/101‑1 (MQ=255)
                   gCCGTCTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGCACCGCCGCCTGCTTGGCGAg                                                                              <  2:170407/101‑1 (MQ=255)
                   gCCGTCTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAg                                                                              <  2:3980671/101‑1 (MQ=255)
                   gCCGTCTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAg                                                                              <  1:1035712/101‑1 (MQ=255)
                   gCCGTCTGATGACGCGGCGGGCTCTGCGCCGCG.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCAGCCCGCCCCGAGTCGAACCGCCGCCTGCTTGACGAg                                                                              <  2:222291/101‑1 (MQ=255)
                    ccGTCTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGc                                                                             <  1:1233568/101‑1 (MQ=255)
                      gTCTGATGACGCTGCGGGCGCTGCGCCGCC.GGCTCGAGGAAGGGGGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGGACCGCCGCCGGCTTGACGAGCTg                                                                           >  1:2277157/1‑101 (MQ=255)
                        cTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGAt                                                                         >  1:3305971/1‑101 (MQ=255)
                        cTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGAt                                                                         <  2:3864531/101‑1 (MQ=255)
                         tgatgaCGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATc                                                                        >  1:574140/1‑101 (MQ=255)
                         tgatgaCGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATc                                                                        <  1:4512616/101‑1 (MQ=255)
                          gatgaCGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATcc                                                                       >  2:2116240/1‑101 (MQ=255)
                           atgaCGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATccc                                                                      <  1:1904963/101‑1 (MQ=255)
                           atgaCGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATccc                                                                      >  1:3643127/1‑101 (MQ=255)
                            tgccGCTGCGGGCGTTG.GCCGCCTTGCTCGCGGAAGTGTTCAATCTTTCCAACCCCGCCCGCGGCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATcccc                                                                     <  2:4508581/98‑1 (MQ=255)
                            tgaCGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATcccc                                                                     <  1:955779/101‑1 (MQ=255)
                            tgaCGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATcccc                                                                     <  1:1460872/101‑1 (MQ=255)
                             gccGCTGCGGGCGCTGCGCCGCC.TGCCGGAGGAAGTGTGCAACCTCTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCg                                                                    <  1:4242238/99‑1 (MQ=255)
                             gaCGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCg                                                                    >  2:2021131/1‑101 (MQ=255)
                             gaCGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCg                                                                    <  2:3105696/101‑1 (MQ=255)
                             gaCGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCg                                                                    <  1:4691071/101‑1 (MQ=255)
                             gaCGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCg                                                                    <  1:3535435/101‑1 (MQ=255)
                               cGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGAc                                                                  >  2:2744384/1‑101 (MQ=255)
                               cGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGCGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGAc                                                                  >  2:431896/1‑101 (MQ=255)
                                gcggCGGGCGCTGCGCCGCC.GGCTCGAGGAAGTGTGCAACCTTTCCGCTCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACa                                                                 <  2:734773/98‑1 (MQ=255)
                                 cTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACaa                                                                >  2:2728855/1‑101 (MQ=255)
                                 cTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACaa                                                                <  1:3725507/101‑1 (MQ=255)
                                 cTGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGGGTACAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGAcca                                                                >  1:4146103/1‑99 (MQ=255)
                                  tGCGGGCGCTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGCCGAGCTGATCCCCGACAAg                                                               >  1:156229/1‑101 (MQ=255)
                                         gcTGCGCCGCC.TGCTGGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCgg                                                        <  2:3704108/101‑1 (MQ=255)
                                         gcTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCgg                                                        >  2:4350018/1‑101 (MQ=255)
                                          cTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGt                                                       >  1:337999/1‑101 (MQ=255)
                                          cTGCGCCGCC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGt                                                       >  2:2791767/1‑101 (MQ=255)
                                             cgccgcC.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGa                                                    <  2:2600473/101‑1 (MQ=255)
                                               ccgcc.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATc                                                  >  2:257815/1‑101 (MQ=255)
                                                cccc.TGCTCGAGGAAGTGTGCACCCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATct                                                 <  1:3947509/99‑1 (MQ=255)
                                                   c.TGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgct                                              <  2:1105591/101‑1 (MQ=255)
                                                     tGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgctg                                             <  1:1196799/101‑1 (MQ=255)
                                                      gCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgctgc                                            >  1:2772601/1‑101 (MQ=255)
                                                       cTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgctgct                                           <  1:4909658/101‑1 (MQ=255)
                                                        tCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgctgctg                                          <  2:790645/101‑1 (MQ=255)
                                                         cGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTgg                                         >  2:141894/1‑101 (MQ=255)
                                                         cGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTgg                                         >  1:94134/1‑101 (MQ=255)
                                                         cGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTgg                                         >  1:2405191/1‑101 (MQ=255)
                                                            ggAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCgg                                      >  2:4178904/1‑101 (MQ=255)
                                                            ggAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCgg                                      >  2:5012222/1‑101 (MQ=255)
                                                              aagtgtGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtg                                    >  2:1675172/3‑101 (MQ=255)
                                                              aagtgtGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtg                                    <  2:3262665/99‑1 (MQ=255)
                                                              aagtgtGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtg                                    >  2:2383904/3‑101 (MQ=255)
                                                               agtgtGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtgc                                   >  2:4694449/2‑101 (MQ=255)
                                                                gtgtGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtgct                                  <  1:2049502/101‑1 (MQ=255)
                                                                 tgtgCAACCTTCCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtgctg                                 <  1:3660205/101‑1 (MQ=255)
                                                                  gtgCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACCAGGTTCCGGTCGATCTGCTGCTGGCGGtgctgc                                >  1:4303882/1‑101 (MQ=255)
                                                                   tgCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCg                               <  1:2021617/101‑1 (MQ=255)
                                                                    gCAACCTTTCCGGTCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGt                              >  1:2746210/1‑101 (MQ=255)
                                                                    gCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGt                              >  1:221931/1‑101 (MQ=255)
                                                                     cAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTc                             >  2:3076358/1‑101 (MQ=255)
                                                                       aCCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctg                           >  2:2139764/1‑101 (MQ=255)
                                                                       aCCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctg                           >  1:2110161/1‑101 (MQ=255)
                                                                        ccTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgg                          >  1:3182025/1‑100 (MQ=255)
                                                                          tttCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctg                        >  2:3015272/1‑101 (MQ=255)
                                                                          tttCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctg                        >  2:4881303/1‑101 (MQ=255)
                                                                          tttCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctg                        <  2:3518602/101‑1 (MQ=255)
                                                                           ttCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctgc                       >  1:2457245/1‑101 (MQ=255)
                                                                           ttCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctgc                       >  1:3780338/1‑101 (MQ=255)
                                                                            tCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctgct                      >  1:753439/1‑101 (MQ=255)
                                                                            tCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGGGCTGCGTctgctgct                      >  2:3440526/1‑101 (MQ=255)
                                                                            tCCGACCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctgct                      <  2:2309899/101‑1 (MQ=255)
                                                                              cgctCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTgg                    <  1:3816823/98‑1 (MQ=255)
                                                                              cGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTgg                    >  2:2870277/1‑101 (MQ=255)
                                                                               gATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGga                   >  2:4524682/1‑101 (MQ=255)
                                                                               gATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGga                   <  2:63755/101‑1 (MQ=255)
                                                                               gATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGCCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGga                   >  1:5039759/1‑101 (MQ=255)
                                                                                 tCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGgaag                 <  1:608682/101‑1 (MQ=255)
                                                                                 cattgcccgcGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGgaag                 >  2:4421384/5‑101 (MQ=255)
                                                                                  cccgcccgCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGgaaga                <  2:715721/101‑1 (MQ=255)
                                                                                     gcccgcGCCGAGTCGACCCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGGGCTGCGTCTGCTGCTGGAAGAACg             <  1:1816721/101‑1 (MQ=255)
                                                                                       ccgcGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACggg           >  2:1366145/1‑101 (MQ=255)
                                                                                           gcCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTc       <  2:3305709/101‑1 (MQ=255)
                                                                                            ccGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCg      <  1:1142046/101‑1 (MQ=255)
                                                                                            ccGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCg      <  2:337946/101‑1 (MQ=255)
                                                                                            ccGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCg      >  1:2902277/1‑101 (MQ=255)
                                                                                                gTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGATcc  >  2:4469726/1‑101 (MQ=255)
                                                                                                gTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGATcc  <  2:3788452/101‑1 (MQ=255)
                                                                                                   |                                                                                                 
GTGATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCC.TGCTCGAGGACGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCTAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGATCC  >  NC_014034/66289‑66484

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG